7FAB

CRYSTAL STRUCTURE OF HUMAN IMMUNOGLOBULIN FRAGMENT FAB NEW REFINED AT 2.0 ANGSTROMS RESOLUTION

Replaces:  3FABReplaces:  1FABReplaces:  2FAB

Summary for 7FAB

• Entry DOI: 10.2210/pdb7fab/pdb
• Descriptor: IGG1-LAMBDA NEW FAB (LIGHT CHAIN), IGG1-LAMBDA NEW FAB (HEAVY CHAIN) (3 entities in total)
• Functional Keywords: immunoglobulin, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 45060.44

Authors

Saul, F.,Poljak, R.J. (deposition date: 1991-11-20, release date: 1994-01-31, Last modification date: 2024-10-16)

Primary citation

Saul, F.A.,Poljak, R.J.
Crystal structure of human immunoglobulin fragment Fab New refined at 2.0 A resolution.
Proteins, 14:363-371, 1992

PubMed Abstract: The three-dimensional structure of the human immunoglobulin fragment Fab New (IgG1, lambda) has been refined to a crystallographic R-factor of 16.9% to 2 A resolution. Rms deviations of the final model from ideal geometry are 0.014 A for bond distances and 3.03 degrees for bond angles. Refinement was based on a new X-ray data set including 28,301 reflections with F > 2.5 sigma(F) from 6.0 to 2.0 A resolution. The starting model for the refinement procedure reported here is from the Brookhaven Protein Data Bank entry 3FAB (rev. 1981). Differences between the initial and final models include modified polypeptide-chain folding in the third complementarity-determining region (CDR3) and the third framework region (FR3) of VH and in some exposed loops of CL and CH1. Amino acid sequence changes were determined at a number of positions by inspection of difference electron density maps. The incorporation of amino acid sequence changes results in an improved VH framework model for the "humanization" of monoclonal antibodies.

PubMed: 1438175
DOI: 10.1002/prot.340140305

Experimental method

X-RAY DIFFRACTION (2 Å)