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Yorodumi- EMDB-6442: CryoEM structure of endogenously assembled Tetrahymena telomerase... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-6442 | |||||||||
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Title | CryoEM structure of endogenously assembled Tetrahymena telomerase holoenzyme at 9.4 Angstrom resolution | |||||||||
Map data | Tetrahymena telomerase holoenzyme, map refined using a spherical mask | |||||||||
Sample |
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Keywords | telomerase / telomere / Tetrahymena | |||||||||
Biological species | Tetrahymena thermophila (eukaryote) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 9.4 Å | |||||||||
Authors | Jiang J / Chan H / Cash DD / Miracco EJ / Loo RRO / Upton HE / Cascio D / Johnson RO / Collins K / Loo JA ...Jiang J / Chan H / Cash DD / Miracco EJ / Loo RRO / Upton HE / Cascio D / Johnson RO / Collins K / Loo JA / Zhou ZH / Feigon J | |||||||||
Citation | Journal: Science / Year: 2015 Title: Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions. Authors: Jiansen Jiang / Henry Chan / Darian D Cash / Edward J Miracco / Rachel R Ogorzalek Loo / Heather E Upton / Duilio Cascio / Reid O'Brien Johnson / Kathleen Collins / Joseph A Loo / Z Hong Zhou / Juli Feigon / Abstract: Telomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-ends, using an integral telomerase RNA (TER) and telomerase reverse transcriptase (TERT). We report the ...Telomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-ends, using an integral telomerase RNA (TER) and telomerase reverse transcriptase (TERT). We report the cryo-electron microscopy structure of Tetrahymena telomerase at ~9 angstrom resolution. In addition to seven known holoenzyme proteins, we identify two additional proteins that form a complex (TEB) with single-stranded telomere DNA-binding protein Teb1, paralogous to heterotrimeric replication protein A (RPA). The p75-p45-p19 subcomplex is identified as another RPA-related complex, CST (CTC1-STN1-TEN1). This study reveals the paths of TER in the TERT-TER-p65 catalytic core and single-stranded DNA exit; extensive subunit interactions of the TERT essential N-terminal domain, p50, and TEB; and other subunit identities and structures, including p19 and p45C crystal structures. Our findings provide structural and mechanistic insights into telomerase holoenzyme function. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_6442.map.gz | 7.3 MB | EMDB map data format | |
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Header (meta data) | emd-6442-v30.xml emd-6442.xml | 15.6 KB 15.6 KB | Display Display | EMDB header |
Images | 400_6442.gif 80_6442.gif | 55 KB 4.3 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-6442 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-6442 | HTTPS FTP |
-Validation report
Summary document | emd_6442_validation.pdf.gz | 79.2 KB | Display | EMDB validaton report |
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Full document | emd_6442_full_validation.pdf.gz | 78.3 KB | Display | |
Data in XML | emd_6442_validation.xml.gz | 494 B | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-6442 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-6442 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_6442.map.gz / Format: CCP4 / Size: 7.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Tetrahymena telomerase holoenzyme, map refined using a spherical mask | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.72 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Telomerase holoenzyme from Tetrahymena thermophila
Entire | Name: Telomerase holoenzyme from Tetrahymena thermophila |
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Components |
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-Supramolecule #1000: Telomerase holoenzyme from Tetrahymena thermophila
Supramolecule | Name: Telomerase holoenzyme from Tetrahymena thermophila / type: sample / ID: 1000 / Oligomeric state: heterodecamer / Number unique components: 1 |
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Molecular weight | Theoretical: 560 KDa |
-Macromolecule #1: Telomerase holoenzyme
Macromolecule | Name: Telomerase holoenzyme / type: protein_or_peptide / ID: 1 / Name.synonym: Telomerase / Number of copies: 1 / Oligomeric state: heterodecamer / Recombinant expression: No / Database: NCBI |
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Source (natural) | Organism: Tetrahymena thermophila (eukaryote) / synonym: Tetrahymena / Organelle: nucleus |
Molecular weight | Theoretical: 560 KDa |