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- PDB-4d10: Crystal structure of the COP9 signalosome -

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Basic information

Entry
Database: PDB / ID: 4d10
TitleCrystal structure of the COP9 signalosome
Components(COP9 SIGNALOSOME COMPLEX SUBUNIT ...) x 8
KeywordsSIGNALING PROTEIN
Function / homology
Function and homology information


regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / COP9 signalosome assembly / trophectodermal cell proliferation / macrophage migration inhibitory factor binding / regulation of IRE1-mediated unfolded protein response / exosomal secretion / deNEDDylase activity / GTPase inhibitor activity / eukaryotic translation initiation factor 3 complex / regulation of protein neddylation ...regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / COP9 signalosome assembly / trophectodermal cell proliferation / macrophage migration inhibitory factor binding / regulation of IRE1-mediated unfolded protein response / exosomal secretion / deNEDDylase activity / GTPase inhibitor activity / eukaryotic translation initiation factor 3 complex / regulation of protein neddylation / protein deneddylation / COP9 signalosome / metal-dependent deubiquitinase activity / activation of NF-kappaB-inducing kinase activity / protein neddylation / Hydrolases; Acting on peptide bonds (peptidases) / RHOBTB1 GTPase cycle / inner cell mass cell proliferation / protein deubiquitination / skeletal muscle cell differentiation / regulation of JNK cascade / response to light stimulus / JNK cascade / translation initiation factor activity / post-translational protein modification / DNA Damage Recognition in GG-NER / neuron differentiation / Formation of TC-NER Pre-Incision Complex / transcription corepressor activity / metallopeptidase activity / positive regulation of DNA-binding transcription factor activity / synaptic vesicle / Cargo recognition for clathrin-mediated endocytosis / cell junction / Neddylation / ubiquitin-dependent protein catabolic process / in utero embryonic development / transcription by RNA polymerase II / transcription coactivator activity / regulation of cell cycle / nuclear speck / translation / negative regulation of cell population proliferation / protein phosphorylation / chromatin / negative regulation of apoptotic process / perinuclear region of cytoplasm / negative regulation of transcription by RNA polymerase II / enzyme binding / signal transduction / positive regulation of transcription by RNA polymerase II / proteolysis / extracellular exosome / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
COP9 signalosome, subunit CSN8 / COP9 signalosome complex subunit 7, helix I / : / : / COP9 signalosome complex subunit 7a helix I domain / COP9 signalosome complex subunit 1, C-terminal helix / COP9 signalosome complex subunit 3-like, C-terminal helix / COP9 signalosome subunit 6 / Cop9 signalosome subunit 5 C-terminal domain / COP9 signalosome complex subunit 4, helix turn helix domain ...COP9 signalosome, subunit CSN8 / COP9 signalosome complex subunit 7, helix I / : / : / COP9 signalosome complex subunit 7a helix I domain / COP9 signalosome complex subunit 1, C-terminal helix / COP9 signalosome complex subunit 3-like, C-terminal helix / COP9 signalosome subunit 6 / Cop9 signalosome subunit 5 C-terminal domain / COP9 signalosome complex subunit 4, helix turn helix domain / Cop9 signalosome subunit 5 C-terminal domain / CSN4/RPN5/eIF3a helix turn helix domain / Eukaryotic translation initiation factor 3 subunit M eIF3m/COP9 signalosome complex subunit 7 COPS7 / Cytidine Deaminase, domain 2 / Cytidine Deaminase; domain 2 / 26S proteasome regulatory subunit Rpn7, N-terminal / 26S proteasome regulatory subunit Rpn7/COP9 signalosome complex subunit 1 / 26S proteasome subunit RPN7 / 26S Proteasome non-ATPase regulatory subunit 12/COP9 signalosome complex subunit 4 / PCI/PINT associated module / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / JAB/MPN domain / JAB1/MPN/MOV34 metalloenzyme domain / MPN domain / MPN domain profile. / Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain / Tetratricopeptide-like helical domain superfamily / Arc Repressor Mutant, subunit A / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
COP9 signalosome complex subunit 2 / COP9 signalosome complex subunit 1 / COP9 signalosome complex subunit 6 / COP9 signalosome complex subunit 5 / COP9 signalosome complex subunit 8 / COP9 signalosome complex subunit 4 / COP9 signalosome complex subunit 7a / COP9 signalosome complex subunit 3
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MIRAS / Resolution: 3.8 Å
AuthorsBunker, R.D. / Lingaraju, G.M. / Thoma, N.H.
CitationJournal: Nature / Year: 2014
Title: Crystal Structure of the Human Cop9 Signalosome
Authors: Lingaraju, G.M. / Bunker, R.D. / Cavadini, S. / Hess, D. / Hassiepen, U. / Renatus, M. / Fischer, E.S. / Thoma, N.H.
History
DepositionApr 30, 2014Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jul 23, 2014Provider: repository / Type: Initial release
Revision 1.1Jul 30, 2014Group: Database references
Revision 1.2Aug 20, 2014Group: Database references
Revision 1.3Mar 6, 2019Group: Data collection / Experimental preparation / Other
Category: exptl_crystal_grow / pdbx_database_proc / pdbx_database_status
Item: _exptl_crystal_grow.method / _pdbx_database_status.recvd_author_approval
Revision 1.4Mar 27, 2019Group: Data collection / Source and taxonomy / Category: entity_src_gen / struct_biol
Item: _entity_src_gen.pdbx_host_org_cell_line / _entity_src_gen.pdbx_host_org_strain

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: COP9 SIGNALOSOME COMPLEX SUBUNIT 1
B: COP9 SIGNALOSOME COMPLEX SUBUNIT 2
C: COP9 SIGNALOSOME COMPLEX SUBUNIT 3
D: COP9 SIGNALOSOME COMPLEX SUBUNIT 4
E: COP9 SIGNALOSOME COMPLEX SUBUNIT 5
F: COP9 SIGNALOSOME COMPLEX SUBUNIT 6
G: COP9 SIGNALOSOME COMPLEX SUBUNIT 7A
H: COP9 SIGNALOSOME COMPLEX SUBUNIT 8
I: COP9 SIGNALOSOME COMPLEX SUBUNIT 1
J: COP9 SIGNALOSOME COMPLEX SUBUNIT 2
K: COP9 SIGNALOSOME COMPLEX SUBUNIT 3
L: COP9 SIGNALOSOME COMPLEX SUBUNIT 4
M: COP9 SIGNALOSOME COMPLEX SUBUNIT 5
N: COP9 SIGNALOSOME COMPLEX SUBUNIT 6
O: COP9 SIGNALOSOME COMPLEX SUBUNIT 7A
P: COP9 SIGNALOSOME COMPLEX SUBUNIT 8
hetero molecules


Theoretical massNumber of molelcules
Total (without water)646,20318
Polymers646,07216
Non-polymers1312
Water0
1
I: COP9 SIGNALOSOME COMPLEX SUBUNIT 1
J: COP9 SIGNALOSOME COMPLEX SUBUNIT 2
K: COP9 SIGNALOSOME COMPLEX SUBUNIT 3
L: COP9 SIGNALOSOME COMPLEX SUBUNIT 4
M: COP9 SIGNALOSOME COMPLEX SUBUNIT 5
N: COP9 SIGNALOSOME COMPLEX SUBUNIT 6
O: COP9 SIGNALOSOME COMPLEX SUBUNIT 7A
P: COP9 SIGNALOSOME COMPLEX SUBUNIT 8
hetero molecules


Theoretical massNumber of molelcules
Total (without water)323,1029
Polymers323,0368
Non-polymers651
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area37150 Å2
ΔGint-248.8 kcal/mol
Surface area114490 Å2
MethodPISA
2
A: COP9 SIGNALOSOME COMPLEX SUBUNIT 1
B: COP9 SIGNALOSOME COMPLEX SUBUNIT 2
C: COP9 SIGNALOSOME COMPLEX SUBUNIT 3
D: COP9 SIGNALOSOME COMPLEX SUBUNIT 4
E: COP9 SIGNALOSOME COMPLEX SUBUNIT 5
F: COP9 SIGNALOSOME COMPLEX SUBUNIT 6
G: COP9 SIGNALOSOME COMPLEX SUBUNIT 7A
H: COP9 SIGNALOSOME COMPLEX SUBUNIT 8
hetero molecules


Theoretical massNumber of molelcules
Total (without water)323,1029
Polymers323,0368
Non-polymers651
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area37050 Å2
ΔGint-252 kcal/mol
Surface area122820 Å2
MethodPISA
Unit cell
Length a, b, c (Å)151.615, 151.615, 343.069
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number144
Space group name H-MP31
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11I
21A
12J
22B
13K
23C
14L
24D
15M
25E
16N
26F
17O
27G
18P
28H

NCS domain segments:
Dom-IDComponent-IDEns-IDRefine codeAuth asym-IDAuth seq-ID
1010I77 - 505
2010A77 - 505
1020J30 - 443
2020B30 - 443
1030K3 - 403
2030C3 - 403
1040L182 - 405
2040D182 - 405
1050M24 - 333
2050E24 - 333
1060N29 - 316
2060F29 - 316
1070O8 - 215
2070G8 - 215
1080P11 - 209
2080H11 - 209

NCS ensembles :
ID
1
2
3
4
5
6
7
8

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Components

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COP9 SIGNALOSOME COMPLEX SUBUNIT ... , 8 types, 16 molecules AIBJCKDLEMFNGOHP

#1: Protein COP9 SIGNALOSOME COMPLEX SUBUNIT 1 / / SGN1 / SIGNALOSOME SUBUNIT 1 / G PROTEIN PATHWAY SUPPRESSOR 1 / GPS-1 / JAB1-CONTAINING SIGNALOSOME ...SGN1 / SIGNALOSOME SUBUNIT 1 / G PROTEIN PATHWAY SUPPRESSOR 1 / GPS-1 / JAB1-CONTAINING SIGNALOSOME SUBUNIT 1 / PROTEIN MFH


Mass: 54222.805 Da / Num. of mol.: 2 / Fragment: RESIDUES 52-527
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: Q13098
#2: Protein COP9 SIGNALOSOME COMPLEX SUBUNIT 2 / / SGN2 / SIGNALOSOME SUBUNIT 2 / ALIEN HOMOLOG / JAB1-CONTAINING SIGNALOSOME SUBUNIT 2 / THYROID ...SGN2 / SIGNALOSOME SUBUNIT 2 / ALIEN HOMOLOG / JAB1-CONTAINING SIGNALOSOME SUBUNIT 2 / THYROID RECEPTOR-INTERACTING PROTEIN 15 / TR- INTERACTING PROTEIN 15 / TRIP-15


Mass: 51992.922 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: P61201
#3: Protein COP9 SIGNALOSOME COMPLEX SUBUNIT 3 / / SGN3 / SIGNALOSOME SUBUNIT 3 / JAB1-CONTAINING SIGNALOSOME SUBUNIT 3


Mass: 47924.008 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: Q9UNS2
#4: Protein COP9 SIGNALOSOME COMPLEX SUBUNIT 4 / / SGN4 / SIGNALOSOME SUBUNIT 4 / JAB1-CONTAINING SIGNALOSOME SUBUNIT 4


Mass: 46651.039 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: Q9BT78
#5: Protein COP9 SIGNALOSOME COMPLEX SUBUNIT 5 / / SGN5 / SIGNALOSOME SUBUNIT 5 / JUN ACTIVATION DOMAIN-BINDING PROTEIN 1


Mass: 37621.742 Da / Num. of mol.: 2 / Fragment: RESIDUES 1-423
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper)
References: UniProt: Q92905, Hydrolases; Acting on peptide bonds (peptidases)
#6: Protein COP9 SIGNALOSOME COMPLEX SUBUNIT 6 / / SGN6 / SIGNALOSOME SUBUNIT 6 / JAB1-CONTAINING SIGNALOSOME SUBUNIT 6 / MOV34 HOMOLOG / VPR- ...SGN6 / SIGNALOSOME SUBUNIT 6 / JAB1-CONTAINING SIGNALOSOME SUBUNIT 6 / MOV34 HOMOLOG / VPR-INTERACTING PROTEIN / HVIP


Mass: 36531.746 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: Q7L5N1
#7: Protein COP9 SIGNALOSOME COMPLEX SUBUNIT 7A / / SGN7A / SIGNALOSOME SUBUNIT 7A / DERMAL PAPILLA-DERIVED PROTEIN 10 / JAB1-CONTAINING SIGNALOSOME SUBUNIT 7A


Mass: 24649.197 Da / Num. of mol.: 2 / Fragment: RESIDUES 1-218
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: Q9UBW8
#8: Protein COP9 SIGNALOSOME COMPLEX SUBUNIT 8 / / SGN8 / SIGNALOSOME SUBUNIT 8 / COP9 HOMOLOG / HCOP9 / JAB1-CONT AINING SIGNALOSOME SUBUNIT 8 / SGN8 ...SGN8 / SIGNALOSOME SUBUNIT 8 / COP9 HOMOLOG / HCOP9 / JAB1-CONT AINING SIGNALOSOME SUBUNIT 8 / SGN8 / SIGNALOSOME SUBUNIT 8 / COP9 HO MOLOG / JAB1-CONTAINING SIGNALOSOME SUBUNIT 8


Mass: 23442.697 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): High Five / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: Q99627

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Non-polymers , 1 types, 2 molecules

#9: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn

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Details

Sequence detailsCHAINS A AND I MAPS TO UNIPROT Q13098 ISOFORM 4

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.49 Å3/Da / Density % sol: 65 % / Description: NONE
Crystal growMethod: vapor diffusion / pH: 5.4
Details: CRYSTALS GROWN BY VAPOR DIFFUSION BY MIXING 9.3 MG/ML PROTEIN IN 50 MM HEPES PH 7.4, 200 MM NACL, 2 MM EQUALLY WITH 12% PEG 6000, 100 MM TRISODIUM CITRATE PH 5.4, 0.1 M LI2SO4, 10 MM UREA.

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 1
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 18, 2011 / Details: DYNAMICALLY BENDABLE MIRROR
RadiationMonochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
Reflection twin
Crystal-IDIDOperatorDomain-IDFraction
11H, K, L10.636
11K, H, -L20.364
ReflectionResolution: 3.8→52 Å / Num. obs: 86875 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 5.8 % / Rmerge(I) obs: 0.12 / Net I/σ(I): 9.3
Reflection shellResolution: 3.8→3.81 Å / Redundancy: 5.8 % / Mean I/σ(I) obs: 1 / % possible all: 100

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Processing

Software
NameVersionClassification
REFMAC5.8.0071refinement
XDSdata reduction
XDSAIMLESSdata scaling
SHARPphasing
RefinementMethod to determine structure: MIRAS
Starting model: NONE

Resolution: 3.8→50.87 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.941 / SU B: 45.893 / SU ML: 0.322 / Cross valid method: THROUGHOUT / ESU R Free: 0.128 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.2275 1656 1.9 %RANDOM
Rwork0.1994 ---
obs0.19993 85161 99.94 %-
Solvent computationIon probe radii: 1 Å / Shrinkage radii: 1 Å / VDW probe radii: 1.1 Å / Solvent model: MASK
Displacement parametersBiso mean: 189.557 Å2
Refinement stepCycle: LAST / Resolution: 3.8→50.87 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms39974 0 2 0 39976
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0080.01940705
X-RAY DIFFRACTIONr_bond_other_d0.0020.0239458
X-RAY DIFFRACTIONr_angle_refined_deg1.0951.96454972
X-RAY DIFFRACTIONr_angle_other_deg0.826390737
X-RAY DIFFRACTIONr_dihedral_angle_1_deg7.71654967
X-RAY DIFFRACTIONr_dihedral_angle_2_deg42.7624.7331927
X-RAY DIFFRACTIONr_dihedral_angle_3_deg16.297157505
X-RAY DIFFRACTIONr_dihedral_angle_4_deg13.9715228
X-RAY DIFFRACTIONr_chiral_restr0.0590.26207
X-RAY DIFFRACTIONr_gen_planes_refined0.0050.0245822
X-RAY DIFFRACTIONr_gen_planes_other0.0020.029282
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it1.54415.25219958
X-RAY DIFFRACTIONr_mcbond_other1.54315.25219957
X-RAY DIFFRACTIONr_mcangle_it2.75422.87224895
X-RAY DIFFRACTIONr_mcangle_other2.75422.87224896
X-RAY DIFFRACTIONr_scbond_it1.13515.31820747
X-RAY DIFFRACTIONr_scbond_other1.13515.31820747
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other2.08922.94230077
X-RAY DIFFRACTIONr_long_range_B_refined7.593161331
X-RAY DIFFRACTIONr_long_range_B_other7.593161331
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05

Ens-IDDom-IDAuth asym-IDNumberRms dev position (Å)
11I251670.04
12A251670.04
21J251510.04
22B251510.04
31K238740.04
32C238740.04
41L129310.09
42D129310.09
51M173230.05
52E173230.05
61N155190.05
62F155190.05
71O123220.05
72G123220.05
81P100900.04
82H100900.04
LS refinement shellResolution: 3.8→3.898 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.278 121 -
Rwork0.29 6253 -
obs--99.72 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.25410.302-0.03460.4516-0.04960.060.1486-0.0965-0.06510.2225-0.0849-0.03670.01430.1589-0.06360.6667-0.105-0.11720.5066-0.12590.119930.846853.520854.306
20.3732-0.4603-0.00650.7450.02940.0632-0.108-0.0137-0.0273-0.066-0.01210.2186-0.08210.03210.12010.41410.0745-0.31480.3957-0.28340.6183-7.358698.802925.6237
32.6849-0.85660.39380.2861-0.1370.1106-0.06640.16540.16170.03010.0268-0.09140.0141-0.0490.03960.1144-0.01970.0160.5972-0.1320.5508-40.082170.600815.327
40.35270.24320.1750.8240.48270.49380.24210.0374-0.01220.2321-0.031-0.10050.02440.1996-0.2110.5642-0.29370.04080.564-0.21450.16453.399287.633149.7854
51.62040.52120.04870.5362-0.17240.1665-0.07830.1564-0.25280.00120.1805-0.04340.1092-0.0393-0.10220.7073-0.2286-0.0130.5583-0.04530.1381-17.81424.941922.389
60.9452-0.0266-0.37510.85740.33210.31450.1842-0.2018-0.07090.2513-0.1808-0.00560.07270.101-0.00340.8976-0.32210.01680.4092-0.06090.02452.597933.84967.2814
71.0745-0.6378-0.28762.2061-0.39090.27450.06740.1457-0.21270.0935-0.1483-0.05790.05780.00910.08080.58240.1082-0.12360.341-0.28160.332730.124214.595927.6211
82.78851.51872.02371.42161.13722.49260.08880.0088-0.1281-0.0398-0.0528-0.21780.29270.3198-0.0360.2340.0703-0.09920.5925-0.15730.266759.563568.290825.3496
90.0229-0.05390.00050.7042-0.12850.24750.08430.00280.0044-0.10950.00410.1549-0.02830.3294-0.08840.50940.02640.02490.595-0.16790.14944.245242.1244-39.6411
100.34630.39650.1011.15790.05880.0592-0.09490.0922-0.13330.10140.22350.22630.02730.0707-0.12860.5558-0.06380.08470.3535-0.18780.43585.6307-5.1091-12.2322
112.52230.6948-0.32220.2103-0.06560.18510.035-0.0136-0.097-0.0453-0.0098-0.022-0.072-0.1297-0.02520.25420.0235-0.16440.6772-0.13190.3096-27.424823.0842-1.8652
120.5957-0.008-0.47160.67510.0960.60380.171-0.03970.043-0.0764-0.0248-0.19580.14220.2183-0.14610.47860.395-0.13790.6936-0.2680.168167.33358.2848-35.8154
130.3295-0.0636-0.04560.126-0.1831.0273-0.077-0.183-0.18570.0230.24650.0253-0.5148-0.1395-0.16950.71740.03520.0430.5503-0.02960.263210.297272.0627-12.643
140.3440.27810.3530.86680.27410.480.04240.06530.0055-0.1885-0.07950.0456-0.23420.07050.03710.81110.0417-0.08590.402-0.01710.011416.005161.7447-52.7057
151.39820.4759-0.11290.4997-0.27910.1910.2292-0.31510.11720.1798-0.3119-0.0372-0.11730.12150.08270.631-0.22720.02190.5175-0.22950.224441.92479.8666-11.4149
160.62740.1981-0.65281.3165-0.37041.00070.2172-0.0134-0.00580.2106-0.2679-0.095-0.31530.46730.05070.14-0.0432-0.1020.9597-0.21710.253272.506926.7756-10.5396
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A464 - 505
2X-RAY DIFFRACTION1B412 - 443
3X-RAY DIFFRACTION1C346 - 403
4X-RAY DIFFRACTION1D367 - 406
5X-RAY DIFFRACTION1E250 - 333
6X-RAY DIFFRACTION1F208 - 316
7X-RAY DIFFRACTION1G166 - 216
8X-RAY DIFFRACTION1H194 - 208
9X-RAY DIFFRACTION2A77 - 463
10X-RAY DIFFRACTION3B30 - 411
11X-RAY DIFFRACTION4C3 - 345
12X-RAY DIFFRACTION5D1 - 366
13X-RAY DIFFRACTION6E24 - 249
14X-RAY DIFFRACTION6F29 - 207
15X-RAY DIFFRACTION7G8 - 165
16X-RAY DIFFRACTION8H12 - 167
17X-RAY DIFFRACTION9I464 - 505
18X-RAY DIFFRACTION9J412 - 443
19X-RAY DIFFRACTION9K346 - 403
20X-RAY DIFFRACTION9L367 - 406
21X-RAY DIFFRACTION9M250 - 333
22X-RAY DIFFRACTION9N208 - 316
23X-RAY DIFFRACTION9O166 - 216
24X-RAY DIFFRACTION9P194 - 208
25X-RAY DIFFRACTION10I77 - 463
26X-RAY DIFFRACTION11J30 - 411
27X-RAY DIFFRACTION12K3 - 345
28X-RAY DIFFRACTION13L78 - 366
29X-RAY DIFFRACTION14M24 - 249
30X-RAY DIFFRACTION14N29 - 207
31X-RAY DIFFRACTION15O8 - 165
32X-RAY DIFFRACTION16P12 - 167

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