ジャーナル: Cell Res / 年: 2017 タイトル: Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii. 著者: Zhifei Li / Qiang Guo / Lvqin Zheng / Yongsheng Ji / Yi-Ting Xie / De-Hua Lai / Zhao-Rong Lun / Xun Suo / Ning Gao / 要旨: As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High- ...As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. With cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, Trichomonas vaginalis and Toxoplasma gondii, at resolution of 3.2-3.4 Å. Although the ribosomal proteins from both pathogens are typical members of eukaryotic families, with a co-evolution pattern between certain species-specific insertions/extensions and neighboring ribosomal RNA (rRNA) expansion segments, the sizes of their rRNAs are sharply different. Very interestingly, rRNAs of T. vaginalis are in size comparable to prokaryotic counterparts, with nearly all the eukaryote-specific rRNA expansion segments missing. These structures facilitate the dissection of evolution path for ribosomal proteins and RNAs, and may aid in design of novel translation inhibitors.
#89 - 2007年5月 アコニターゼと鉄調節タンパク質1 (Aconitase and Iron Regulatory Protein 1) 類似性 (1)
#241 - 2020年1月 20年の分子を振り返って (Twenty Years of Molecules) 類似性 (1)
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集合体
登録構造単位
2: 18S RNA A: Ribosomal protein uS2 B: Ribosomal protein eS1 C: Ribosomal protein uS5 D: Ribosomal protein uS3 E: Ribosomal protein eS4 F: Ribosomal protein uS7 G: Ribosomal protein eS6 H: Ribosomal protein eS7 I: Ribosomal protein eS8 J: Ribosomal protein uS4 K: Ribosomal protein eS10 L: Ribosomal protein uS17 M: Ribosomal protein eS12 N: Ribosomal protein uS15 O: Ribosomal protein uS11 P: Ribosomal protein uS19 Q: Ribosomal protein uS9 R: Ribosomal protein eS17 S: Ribosomal protein uS13 T: Ribosomal protein eS19 U: Ribosomal protein uS10 V: Ribosomal protein eS21 W: Ribosomal protein uS8 X: Ribosomal protein uS12 Y: Ribosomal protein eS24 Z: Ribosomal protein eS25 a: Ribosomal protein eS26 b: Ribosomal protein eS27 c: Ribosomal protein eS28 d: Ribosomal protein uS14 e: Ribosomal protein eS30 f: Ribosomal protein eS31 m: Ribosomal protein eL41
Sequences used in this study were derived from Toxoplasma gondii RH strain, which were not ...Sequences used in this study were derived from Toxoplasma gondii RH strain, which were not available at UNIPROT at the time of data processing.
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実験情報
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実験
実験
手法: 電子顕微鏡法
EM実験
試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法
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試料調製
構成要素
名称: Small subunit of Toxoplasma gondii ribosome / タイプ: RIBOSOME / Entity ID: all / 由来: NATURAL