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- EMDB-10760: Mammalian 48S late-stage initiation complex with beta-globin mRNA -

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Basic information

Entry
Database: EMDB / ID: EMD-10760
TitleMammalian 48S late-stage initiation complex with beta-globin mRNA
Map data
Sample
  • Complex: LS48S IC with beta-globin mRNA
    • Complex: LS48S IC
      • RNA: x 2 types
      • Protein or peptide: x 38 types
    • Complex: beta-globin mRNA
      • RNA: x 1 types
  • Ligand: x 3 types
Function / homology
Function and homology information


ribosomal subunit / regulation of translational fidelity / translation initiation factor activity / DNA-(apurinic or apyrimidinic site) lyase / small-subunit processome / ribosomal small subunit biogenesis / cytoplasmic stress granule / cytosolic small ribosomal subunit / cell differentiation / ribosome ...ribosomal subunit / regulation of translational fidelity / translation initiation factor activity / DNA-(apurinic or apyrimidinic site) lyase / small-subunit processome / ribosomal small subunit biogenesis / cytoplasmic stress granule / cytosolic small ribosomal subunit / cell differentiation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / synapse / nucleolus / ATP hydrolysis activity / RNA binding / ATP binding / cytoplasm
Similarity search - Function
RLI, domain 1 / RLI1 / RNase L inhibitor RLI-like, possible metal-binding domain / Possible Fer4-like domain in RNase L inhibitor, RLI / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / IF2a, S1-like domain / Translation initiation factor 2, alpha subunit ...RLI, domain 1 / RLI1 / RNase L inhibitor RLI-like, possible metal-binding domain / Possible Fer4-like domain in RNase L inhibitor, RLI / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / IF2a, S1-like domain / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / Eukaryotic translation initiation factor 2 alpha subunit / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / 4Fe-4S binding domain / Ribosomal protein S26e signature. / S1 domain profile. / Ribosomal protein S21e signature. / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein S19e signature. / 40S Ribosomal protein S10 / Ribosomal protein S6/S6e/A/B/2, conserved site / Plectin/S10, N-terminal / Ribosomal protein S3Ae / Ribosomal S3Ae family / Plectin/S10 domain / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal S3Ae family / Ribosomal protein S17e signature. / Ribosomal protein S7e signature. / Ribosomal protein S6e / Ribosomal protein S3Ae signature. / Ribosomal protein S27e signature. / Ribosomal protein S4e signature. / Ribosomal protein S8e signature. / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S24e / Ribosomal protein S24e / Ribosomal protein S6e signature. / Ribosomal protein S28e signature. / Ribosomal protein S1-like RNA-binding domain / S1 RNA binding domain / S1 domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / Ribosomal protein S2 signature 2. / Ubiquitin domain signature. / Ribosomal protein L30 signature. / Ribosomal protein S3 signature. / Ribosomal protein S10 signature. / Ribosomal protein S14 signature. / Ribosomal protein S7, conserved site / Ribosomal protein S2 signature 1. / Type-2 KH domain profile. / Ribosomal protein S19 signature. / Ribosomal protein S7 signature. / Ribosomal protein S17 signature. / Ribosomal protein S13 signature. / Ribosomal protein S5/S7 / Ribosomal protein S7 domain / Ribosomal protein S7 domain superfamily / Ribosomal protein S13 family profile. / Ribosomal protein S8 signature. / Ribosomal protein S4 signature. / Ribosomal protein S15 signature. / Ribosomal protein S7p/S5e / S4 RNA-binding domain profile. / Ribosomal protein S11 signature. / Ubiquitin domain profile. / Ribosomal protein S9 signature. / Ribosomal protein S12 signature. / Ribosomal protein L23/L15e core domain superfamily / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / Winged helix-like DNA-binding domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Nucleic acid-binding, OB-fold / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Small ribosomal subunit protein eS32 / 40S ribosomal protein S6 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein eS12 / ATP binding cassette subfamily E member 1 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein uS15 ...Small ribosomal subunit protein eS32 / 40S ribosomal protein S6 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein eS12 / ATP binding cassette subfamily E member 1 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS7 / Eukaryotic translation initiation factor 4C / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS11 / Eukaryotic translation initiation factor 2 subunit 1 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein eS4 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Plectin/eS10 N-terminal domain-containing protein / Small ribosomal subunit protein uS17 / 40S ribosomal protein S24 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS14 / Ribosomal protein S5
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit) / Homo sapiens (human) / Rabbit (rabbit)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsBochler A / Simonetti A / Guca E / Hashem Y
Funding supportEuropean Union, France, 2 items
OrganizationGrant numberCountry
European Research Council (ERC)759120European Union
French National Research AgencyANR-14-ACHN-0024 France
CitationJournal: Cell Rep / Year: 2020
Title: Structural Insights into the Mammalian Late-Stage Initiation Complexes.
Authors: Angelita Simonetti / Ewelina Guca / Anthony Bochler / Lauriane Kuhn / Yaser Hashem /
Abstract: In higher eukaryotes, the mRNA sequence in the direct vicinity of the start codon, called the Kozak sequence (CRCCaugG, where R is a purine), is known to influence the rate of the initiation process. ...In higher eukaryotes, the mRNA sequence in the direct vicinity of the start codon, called the Kozak sequence (CRCCaugG, where R is a purine), is known to influence the rate of the initiation process. However, the molecular basis underlying its role remains poorly understood. Here, we present the cryoelectron microscopy (cryo-EM) structures of mammalian late-stage 48S initiation complexes (LS48S ICs) in the presence of two different native mRNA sequences, β-globin and histone 4, at overall resolution of 3 and 3.5 Å, respectively. Our high-resolution structures unravel key interactions from the mRNA to eukaryotic initiation factors (eIFs): 1A, 2, 3, 18S rRNA, and several 40S ribosomal proteins. In addition, we are able to study the structural role of ABCE1 in the formation of native 48S ICs. Our results reveal a comprehensive map of ribosome/eIF-mRNA and ribosome/eIF-tRNA interactions and suggest the impact of mRNA sequence on the structure of the LS48S IC.
History
DepositionMar 12, 2020-
Header (metadata) releaseApr 8, 2020-
Map releaseApr 8, 2020-
UpdateApr 22, 2020-
Current statusApr 22, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6yal
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_10760.png

Downloads & links

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Map

FileDownload / File: emd_10760.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.1 Å
Density
Contour LevelBy AUTHOR: 0.02 / Movie #1: 0.02
Minimum - Maximum-0.07795042 - 0.1473876
Average (Standard dev.)0.00010984154 (±0.0057947584)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 422.40002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.11.11.1
M x/y/z384384384
origin x/y/z0.0000.0000.000
length x/y/z422.400422.400422.400
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS384384384
D min/max/mean-0.0780.1470.000

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Supplemental data

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Sample components

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Entire : LS48S IC with beta-globin mRNA

EntireName: LS48S IC with beta-globin mRNA
Components
  • Complex: LS48S IC with beta-globin mRNA
    • Complex: LS48S IC
      • RNA: initiator methionylated tRNA
      • Protein or peptide: 60s ribosomal protein ul41
      • Protein or peptide: 40S ribosomal protein uS2
      • Protein or peptide: 40S ribosomal protein eS1
      • Protein or peptide: 40S ribosomal protein uS5
      • Protein or peptide: 40S Ribosomal protein uS3
      • Protein or peptide: 40S ribosomal protein eS4
      • Protein or peptide: 40S Ribosomal protein uS7
      • Protein or peptide: 40S ribosomal protein eS6
      • Protein or peptide: 40S ribosomal protein eS7
      • Protein or peptide: 40S ribosomal protein eS8
      • Protein or peptide: 40S ribosomal protein uS4
      • Protein or peptide: 40S ribosomal protein eS10
      • Protein or peptide: 40S ribosomal protein uS17
      • Protein or peptide: 40S ribosomal protein eS12
      • Protein or peptide: 40S ribosomal protein uS15
      • Protein or peptide: 40S ribosomal protein uS11
      • Protein or peptide: 40S ribosomal protein uS9
      • Protein or peptide: 40S ribosomal protein eS17
      • Protein or peptide: 40S ribosomal protein eS19
      • Protein or peptide: 40S ribosomal protein uS10
      • Protein or peptide: 40S ribosomal protein eS21
      • Protein or peptide: 40S ribosomal protein uS8
      • Protein or peptide: 40S ribosomal protein uS12
      • Protein or peptide: 40S ribosomal protein eS24
      • Protein or peptide: 40S ribosomal protein eS26
      • Protein or peptide: 40S ribosomal protein eS27
      • Protein or peptide: 40S ribosomal protein eS28
      • Protein or peptide: 40S ribosomal protein uS14
      • Protein or peptide: 40S ribosomal protein eS31
      • Protein or peptide: ribosomal protein RACK1
      • Protein or peptide: 40S ribosomal protein eS25
      • Protein or peptide: 40S ribosomal protein eS30
      • RNA: 18S ribosomal RNA
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit alpha
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit gamma
      • Protein or peptide: eukaryotic translation initiation factor 1A
      • Protein or peptide: ATP-binding cassette sub-family E member 1 (ABCE1)
      • Protein or peptide: 40S ribosomal protein uS13
      • Protein or peptide: 40S ribosomal protein uS19
    • Complex: beta-globin mRNA
      • RNA: beta-globin mRNA
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: MAGNESIUM ION
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

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Supramolecule #1: LS48S IC with beta-globin mRNA

SupramoleculeName: LS48S IC with beta-globin mRNA / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#41

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Supramolecule #2: LS48S IC

SupramoleculeName: LS48S IC / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#40
Source (natural)Organism: Oryctolagus cuniculus (rabbit)

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Supramolecule #3: beta-globin mRNA

SupramoleculeName: beta-globin mRNA / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #41
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: synthetic construct (others)

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Macromolecule #1: initiator methionylated tRNA

MacromoleculeName: initiator methionylated tRNA / type: rna / ID: 1
Details: A at position 37 is modified (threonyl-carbamoyl-adenosine (t6A))
Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.376625 KDa
SequenceString:
AGCAGAGUGG CGCAGCGGAA GCGUGCUGGG CCCAU(T6A)ACCC AGAGGUCGAU GGAUCGAAAC CAUCCUCUGC UACCA

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Macromolecule #34: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 34 / Details: U at position 1244 is modified / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 601.015688 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC ...String:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC UAAUACAUGC CGACGGCGCU GACUCCCUUU GUGUGGGAUG CGUGCAUUUA UCAGAUCAAA ACCAACCCGG UC AGCCUCC CCGCCGGCCG GGGGGGUGGG GCGGCGGCUU UGGUGACUCU AGAUAACCUC GGGCCGAUCG CAGCCCUCCG UGG CGGCGA CGACCCAUUC GAACGUCUGC CCUAUCAACU UUCGAUGGUA GUCGCCGUGC CUACCAUGGU GACCACGGGU GACG GGGAA UCAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAACGGCUA CCACAUCCAA GGAAGGCAGC AGGCGCGCAA AUUAC CCAC UCCCGACCCG GGGAGGUAGU GACGAAAAAU AACAAUACAG GACUCUUUCG AGGCCCUGUA AUUGGAAUGA GUCCAC UUU AAAUCCUUUA ACGAGGAUCC AUUGGAGGGC AAGUCUGGUC GCAGCAGCCG CGGUAAUUCC AGCUCCAAUA GCGUAUA UU AAAGUUGCUG CAGUUAAAAA GCUCGUAGUU GGAUCUUGUG GAGGGUGCGU AGCGGGCGGU CCGCCGCGAG GCGAGCCA C CGCCCGUCCC CGCCCCUUGC CUCUCGGCGC CCCCUCGAUG CUCUUAGCUG AGUGUCCCGC GGGGCCCGAA GCGUUUACU UUGAAAAAAU UAGAGUGUUC AAAGCAGGCC CGAGCCGCCU GGAUACCGCA GCUAGGAAUA AUGGAAUAGG ACCGCGGUUC UAUUUUGUU GGUUUUCGGA ACUGAGGCCA UGAUUAAGAG GGACGGCCGG GGGCAUUCGU AUUGCGCCGC UAGAGGUGAA A UUCUUGGA CCGGCGCAAG ACGGACCAGA GCGAAAGCAU UUGCCAAGAA UGUUUUCAUU AAUCAAGAAC GAAAGUCGGA GG UUCGAAG ACGAUCAGAU ACCGUCGUAG UUCCGACCAU AAACGAUGCC GACUGGCGAU GCGGCGGCGU UAUUCCCAUG ACC CGCCGG GCAGCUUCCG GGAAACCAAA GUCUUUGGGU UCCGGGGGGA GUAUGGUUGC AAAGCUGAAA CUUAAAGGAA UUGA CGGAA GGGCACCACC AGGAGUGGAG CCUGCGGCUU AAUUUGAC(C4J)C AACACGGGAA ACCUCACCCG GCCCGGACAC G GACAGGAU UGACAGAUUG AUAGCUCUUU CUCGAUUCUG UGGGUGGUGG UGCAUGGCCG UUCUUAGUUG GUGGAGCGAU UU GUCUGGU UAAUUCCGAU AACGAACGAG ACUCUGGCAU GCUAACUAGU UACGCGACCC CCGAGCGGUC GGCGUCCCCC AAC UUCUUA GAGGGACAAG UGGCGUUCAG CCACCCGAGA UUGAGCAAUA ACAGGUCUGU GAUGCCCUUA GAUGUCGGGG CUGC ACGCG CGCUACACUG ACUGGCUCAG CGUGUGCCUA CCCUACGCCG GCAGGCGCGG GUAACCCGUU GAACCCCAUU CGUGA UGGG GAUCGGGGAU UGCAAUUAUU CCCCAUGAAC GAGGAAUUCC CAGUAAGUGC GGGUCAUAAG CUUGCGUUGA UUAAGU CCC UGCCCUUUGU ACACACCGCC CGUCGCUACU ACCGAUUGGA UGGUUUAGUG AGGCCCUCGG AUCGGCCCGC CGGGGUC GG CCCACGGCCC UGGCGGAGCG CUGAGAAGAC GGUCGAACUU GACUAUCUAG AGGAAGUAAA AGUCGUAACA AGGUUUCC G UAGGUGAACC UGCGGAAGGA UCAUUA

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Macromolecule #41: beta-globin mRNA

MacromoleculeName: beta-globin mRNA / type: rna / ID: 41 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.475132 KDa
SequenceString:
ACCUCAAACA GACACCAUGG UGCACCUGAC UCCUGAGGAG AA

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Macromolecule #2: 60s ribosomal protein ul41

MacromoleculeName: 60s ribosomal protein ul41 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 3.473451 KDa
SequenceString:
MRAKWRKKRM RRLKRKRRKM RQRSK

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Macromolecule #3: 40S ribosomal protein uS2

MacromoleculeName: 40S ribosomal protein uS2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.491988 KDa
SequenceString: MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA ...String:
MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE E

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Macromolecule #4: 40S ribosomal protein eS1

MacromoleculeName: 40S ribosomal protein eS1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 29.928967 KDa
SequenceString: MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLHLFC VGFTKKCNNQ IRKTSYAQHQ Q VRQIRKKM ...String:
MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLHLFC VGFTKKCNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

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Macromolecule #5: 40S ribosomal protein uS5

MacromoleculeName: 40S ribosomal protein uS5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.814285 KDa
SequenceString: GKAGDKEWLP VTKLGRLVKD MKIKSLEEIY LFSLPIKESE IIDFFLGAAL KDKVLKIMPV QKQTQAGQRT RFKAFVAIGD YNGHVGLGM KCSKEVATAI RGAIILAKLS IVPVRRGYWG NKIGKPHTVR CKVTGRCGSV LVRLIPAPRG TGIISAPVPK K LLLMAGID ...String:
GKAGDKEWLP VTKLGRLVKD MKIKSLEEIY LFSLPIKESE IIDFFLGAAL KDKVLKIMPV QKQTQAGQRT RFKAFVAIGD YNGHVGLGM KCSKEVATAI RGAIILAKLS IVPVRRGYWG NKIGKPHTVR CKVTGRCGSV LVRLIPAPRG TGIISAPVPK K LLLMAGID DCYTSAGGCT ATLGNFAKAT FDAISKTYSY LTPDLWKETV FTKSPYQEFT DHLVKTHT

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Macromolecule #6: 40S Ribosomal protein uS3

MacromoleculeName: 40S Ribosomal protein uS3 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 26.715344 KDa
SequenceString: MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV ...String:
MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPSGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG GKPEPPAMPQ PV PTA

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Macromolecule #7: 40S ribosomal protein eS4

MacromoleculeName: 40S ribosomal protein eS4 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 29.65892 KDa
SequenceString: MARGPKKHLK RVAAPKHWML DKLTSVFAPR PSTGPHKLRE CLPLIIFLRN KLKYALTGDE VKKICMQRFI KIDGKVRADI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKMNDT I QIDLETGK ...String:
MARGPKKHLK RVAAPKHWML DKLTSVFAPR PSTGPHKLRE CLPLIIFLRN KLKYALTGDE VKKICMQRFI KIDGKVRADI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKMNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

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Macromolecule #8: 40S Ribosomal protein uS7

MacromoleculeName: 40S Ribosomal protein uS7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.525941 KDa
SequenceString:
TPDIKLFGKW STDDVQINDI SLQDYIAVKE KYAKYLPHSA GRYAAKRFRK AQCPIVERLT NSMMMHGRNN GKKLMTVRIV KHAFEIIHL LTGENPLQVL VNAIINSGPR EDSTRIGRAG TVRRQAVDVS PLRRVNQAIW LLCTGAREAA FRNIKTIAEC L ADELINAA KGSSNSYAIK KKDELERVAK SNR

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Macromolecule #9: 40S ribosomal protein eS6

MacromoleculeName: 40S ribosomal protein eS6 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 27.471535 KDa
SequenceString: MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK ...String:
MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSL

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Macromolecule #10: 40S ribosomal protein eS7

MacromoleculeName: 40S ribosomal protein eS7 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.890545 KDa
SequenceString: SSSAKIVKPN GEKPDEFESG ISQALLELEM NSDLKAQLRE LNITAAKEIE VGGGRKAIII FVPVPQLKSF QKIQVRLVRE LEKKFSGKH VVFIAQRRIL PKPTRKSRTK NKQKRPRSRT LTAVHDAILE DLVFPSEIVG KRIRVKLDGS RLIKVHLDKA Q QNNVEHKV ...String:
SSSAKIVKPN GEKPDEFESG ISQALLELEM NSDLKAQLRE LNITAAKEIE VGGGRKAIII FVPVPQLKSF QKIQVRLVRE LEKKFSGKH VVFIAQRRIL PKPTRKSRTK NKQKRPRSRT LTAVHDAILE DLVFPSEIVG KRIRVKLDGS RLIKVHLDKA Q QNNVEHKV ETFSGVYKKL TGKDVNFEFP EFQL

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Macromolecule #11: 40S ribosomal protein eS8

MacromoleculeName: 40S ribosomal protein eS8 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.902871 KDa
SequenceString: GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVGVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG ...String:
GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVGVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG KLLACIASRP GQCGRADGYV LEGKELEFYL RKIKARKG

+
Macromolecule #12: 40S ribosomal protein uS4

MacromoleculeName: 40S ribosomal protein uS4 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.266166 KDa
SequenceString:
WVCRKTYVTP RRPFEKSRLD QELKLIGEYG LRNKREVWRV KFTLAKIRKA ARELLTLDEK DPRRLFEGNA LLRRLVRIGV LDEGKMKLD YILGLKIEDF LERRLQTQVF KLGLAKSIHH ARVLIRQRHI RVRKQVVNIP SFIVRLDSQK HIDFSLRSPY G GGRPGRVK RKNAKKGQGG AGAG

+
Macromolecule #13: 40S ribosomal protein eS10

MacromoleculeName: 40S ribosomal protein eS10 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 11.773953 KDa
SequenceString:
MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSR

+
Macromolecule #14: 40S ribosomal protein uS17

MacromoleculeName: 40S ribosomal protein uS17 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.468826 KDa
SequenceString:
MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

+
Macromolecule #15: 40S ribosomal protein eS12

MacromoleculeName: 40S ribosomal protein eS12 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.538987 KDa
SequenceString:
MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

+
Macromolecule #16: 40S ribosomal protein uS15

MacromoleculeName: 40S ribosomal protein uS15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.128191 KDa
SequenceString:
GRMHAPGKGL SQSALPYRRS VPTWLKLTSD DVKEQIYKLA KKGLTPSQIG VILRDSHGVA QVRFVTGNKI LRILKSKGLA PDLPEDLYH LIKKAVAVRK HLERNRKDKD AKFRLILIES RIHRLARYYK TKRVLPPNWK YESSTASALV A

+
Macromolecule #17: 40S ribosomal protein uS11

MacromoleculeName: 40S ribosomal protein uS11 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.302772 KDa
SequenceString:
MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

+
Macromolecule #18: 40S ribosomal protein uS9

MacromoleculeName: 40S ribosomal protein uS9 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.975753 KDa
SequenceString:
PLQSVQVFGR KKTATAVAHC KRGNGLIKVN GRPLEMIEPR TLQYKLLEPV LLLGKERFAG VDIRVRVKGG GHVAQIYAIR QSISKALVA YYQKYVDEAS KKEIKDILIQ YDRTLLVADP RRCESKKFGG PGARARYQKS YR

+
Macromolecule #19: 40S ribosomal protein eS17

MacromoleculeName: 40S ribosomal protein eS17 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.552119 KDa
SequenceString:
MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

+
Macromolecule #20: 40S ribosomal protein eS19

MacromoleculeName: 40S ribosomal protein eS19 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.812286 KDa
SequenceString:
VTVKDVNQQE FVRALAAFLK KSGKLKVPEW VDTVKLAKHK ELAPYDENWF YTRAASTARH LYLRGGAGVG SMTKIYGGRQ RNGVMPSHF SRGSKSVARR VLQALEGLKM VEKDQDWGRK LTPQGQRDLD RIAGQVAAAK KK

+
Macromolecule #21: 40S ribosomal protein uS10

MacromoleculeName: 40S ribosomal protein uS10 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.398763 KDa
SequenceString:
MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

+
Macromolecule #22: 40S ribosomal protein eS21

MacromoleculeName: 40S ribosomal protein eS21 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 8.896102 KDa
SequenceString:
MQSNAGEFVD LYVPRKCSAS NRIIGAKDHA SIPMNVAEVD KVTGRFNGQS KTYAICGAIR RMGESDDSIL RLAKSHGIVS QN

+
Macromolecule #23: 40S ribosomal protein uS8

MacromoleculeName: 40S ribosomal protein uS8 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.865555 KDa
SequenceString:
MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

+
Macromolecule #24: 40S ribosomal protein uS12

MacromoleculeName: 40S ribosomal protein uS12 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.844666 KDa
SequenceString:
MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

+
Macromolecule #25: 40S ribosomal protein eS24

MacromoleculeName: 40S ribosomal protein eS24 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.434267 KDa
SequenceString:
MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI QEKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KPKE

+
Macromolecule #26: 40S ribosomal protein eS26

MacromoleculeName: 40S ribosomal protein eS26 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.961455 KDa
SequenceString:
MTKKRRNNGR AKKGRGHVQP IRCTNCACCV PKDKAIKKFV IRNIVEAAAV RDISEVSVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPP PPPKPM

+
Macromolecule #27: 40S ribosomal protein eS27

MacromoleculeName: 40S ribosomal protein eS27 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 9.480186 KDa
SequenceString:
MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

+
Macromolecule #28: 40S ribosomal protein eS28

MacromoleculeName: 40S ribosomal protein eS28 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 7.855052 KDa
SequenceString:
MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

+
Macromolecule #29: 40S ribosomal protein uS14

MacromoleculeName: 40S ribosomal protein uS14 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 6.690821 KDa
SequenceString:
MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

+
Macromolecule #30: 40S ribosomal protein eS31

MacromoleculeName: 40S ribosomal protein eS31 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 8.358903 KDa
SequenceString:
KKSYTTPKKN KHKRKKVKLA VLKYYKVDEN GKISRLRREC PSDECGAGVF MASHFDRHYC GKCCLTYCFN K

+
Macromolecule #31: ribosomal protein RACK1

MacromoleculeName: ribosomal protein RACK1 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 34.669113 KDa
SequenceString: TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG ...String:
TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG WDKLVKVWNL ANCKLKTNHI GHTGYLNTVT VSPDGSLCAS GGKDGQAMLW DLNEGKHLYT LDGGDIINAL CF SPNRYWL CAATGPSIKI WDLEGKIIVD ELKQEVISTS SKAEPPQCTS LAWSADGQTL FAGYTDNLVR VWQVTI

+
Macromolecule #32: 40S ribosomal protein eS25

MacromoleculeName: 40S ribosomal protein eS25 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.645028 KDa
SequenceString:
PPKDDKKKKD AGKSAKKDKD PVNKSGGKAK KKKWSKGKVR DKLNNLVLFD KATYDKLCKE VPNYKLITPA VVSERLKIRG SLARAALQE LLSKGLIKLV SKHRAQVIYT RNTKGGDAPA AGEDA

+
Macromolecule #33: 40S ribosomal protein eS30

MacromoleculeName: 40S ribosomal protein eS30 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.498884 KDa
SequenceString:
MQLFVRAQEL HTLEVTGRET VAQIKAHVAS LEGIAPEDQV VLLAGTPLED EATLGQCGVE ALSTLEVAGR MLGGKVHGSL ARVGKVRGQ TLKVAKQEKK KKRTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

+
Macromolecule #35: Eukaryotic translation initiation factor 2 subunit alpha

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit alpha
type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 32.792867 KDa
SequenceString: MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRR VSPEEAIKCE DKFTKSKTVY SILRHVAEVL EYTKDEQLES LFQRTAWVFD DKYKRPGYGA YDAFKHAVSD P SILDSLDL ...String:
MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRR VSPEEAIKCE DKFTKSKTVY SILRHVAEVL EYTKDEQLES LFQRTAWVFD DKYKRPGYGA YDAFKHAVSD P SILDSLDL NEDEREVLIN NINRRLTPQA VKIRADIEVA CYGYEGIDAV KEALRAGLNC STETMPIKIN LIAPPRYVMT TT TLERTEG LSVLNQAMAV IKEKIEEKRG VFNVQMEQVW KTPRRKT

+
Macromolecule #36: Eukaryotic translation initiation factor 2 subunit gamma

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit gamma
type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 45.862441 KDa
SequenceString: QATINIGTIG HVAHGKSTVV KAISGVHTVR FKNELERNIT IKLGYANAKI YKLDDPSCPR PECYRSCGSS TPDEFPTDIP GTKGNFKLV RHVSFVDCPG HDILMATMLN GAAVMDAALL LIAGNESCPQ PQTSEHLAAI EIMKLKHILI LQNKIDLVKE S QAKEQYEQ ...String:
QATINIGTIG HVAHGKSTVV KAISGVHTVR FKNELERNIT IKLGYANAKI YKLDDPSCPR PECYRSCGSS TPDEFPTDIP GTKGNFKLV RHVSFVDCPG HDILMATMLN GAAVMDAALL LIAGNESCPQ PQTSEHLAAI EIMKLKHILI LQNKIDLVKE S QAKEQYEQ ILAFVQGTVA EGAPIIPISA QLKYNIEVVC EYIVKKIPVP PRDFTSEPRL IVIRSFDVNK PGCEVDDLKG GV AGGSILK GVLKVGQEIE VRPGIVSKDS EGKLMCKPIF SKIVSLFAEH NDLQYAAPGG LIGVGTKIDP TLCRADRMVG QVL GAVGAL PEIFTELEIS YFLLRRLLGV RTEGDKKAAK VQKLSKNEVL MVNIGSLSTG GRVSAVKADL GKIVLTNPVC TEVG EKIAL SRRVEKHWRL IGWGQIR

+
Macromolecule #37: eukaryotic translation initiation factor 1A

MacromoleculeName: eukaryotic translation initiation factor 1A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.788764 KDa
SequenceString:
KGKGGKNRRR GKNENESEKR ELVFKEDGQE YAQVIKMLGN GRLEAMCFDG VKRLCHIRGK LRKKVWINTS DIILVGLRDY QDNKADVIL KYNADEARSL KAYGELPEHA KI

+
Macromolecule #38: ATP-binding cassette sub-family E member 1 (ABCE1)

MacromoleculeName: ATP-binding cassette sub-family E member 1 (ABCE1) / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 66.986812 KDa
SequenceString: KLTRIAIVNH DKCKPKKCRQ ECKKSCPVVR MGKLCIEVTP QSKIAWISET LCIGCGICIK KCPFGALSIV NLPSNLEKET THRYCANAF KLHRLPIPRP GEVLGLVGTN GIGKSTALKI LAGKQKPNLG KYDDPPDWQE ILTYFRGSEL QNYFTKILED D LKAIIKPQ ...String:
KLTRIAIVNH DKCKPKKCRQ ECKKSCPVVR MGKLCIEVTP QSKIAWISET LCIGCGICIK KCPFGALSIV NLPSNLEKET THRYCANAF KLHRLPIPRP GEVLGLVGTN GIGKSTALKI LAGKQKPNLG KYDDPPDWQE ILTYFRGSEL QNYFTKILED D LKAIIKPQ YVDQIPKAAK GTVGSILDRK DETKTQAIVC QQLDLTHLKE RNVEDLSGGE LQRFACAVVC IQKADIFMFD EP SSYLDVK QRLKAAITIR SLINPDRYII VVEHDLSVLD YLSDFICCLY GVPSAYGVVT MPFSVREGIN IFLDGYVPTE NLR FRDASL VFKVAETANE EEVKKMCMYK YPGMKKKMGE FELAIVAGEF TDSEIMVMLG ENGTGKTTFI RMLAGRLKPD EGGE VPVLN VSYKPQKISP KSTGSVRQLL HEKIRDAYTH PQFVTDVMKP LQIENIIDQE VQTLSGGELQ RVALALCLGK PADVY LIDE PSAYLDSEQR LMAARVVKRF ILHAKKTAFV VEHDFIMATY LADRVIVFDG IPSKNTVANS PQTLLAGMNK FLSQLE ITF RRDPNNYRPR INKLNSIKDV EQKKSGNYFF LD

+
Macromolecule #39: 40S ribosomal protein uS13

MacromoleculeName: 40S ribosomal protein uS13 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.759777 KDa
SequenceString:
MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

+
Macromolecule #40: 40S ribosomal protein uS19

MacromoleculeName: 40S ribosomal protein uS19 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.049182 KDa
SequenceString:
MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLSRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

+
Macromolecule #42: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 42 / Number of copies: 2 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

+
Macromolecule #43: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 43 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #44: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 44 / Number of copies: 2 / Formula: GNP
Molecular weightTheoretical: 522.196 Da
Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM / 5'-Guanylyl imidodiphosphate

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE / Chamber humidity: 100 %

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 26.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5k0y

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 252000

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Atomic model buiding 1

RefinementProtocol: FLEXIBLE FIT
Output model

PDB-6yal:
Mammalian 48S late-stage initiation complex with beta-globin mRNA

+
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