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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-6788 | |||||||||
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Title | Small subunit of Trichomonas vaginalis ribosome | |||||||||
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![]() | Trichomonas vaginalis ribosome / rRNA / rprotein / RIBOSOME | |||||||||
Function / homology | ![]() positive regulation of translational fidelity / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / protein kinase C binding / mRNA 5'-UTR binding / rRNA processing ...positive regulation of translational fidelity / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / protein kinase C binding / mRNA 5'-UTR binding / rRNA processing / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / ribosome / structural constituent of ribosome / translation / positive regulation of protein phosphorylation / mRNA binding / nucleolus / enzyme binding / RNA binding / zinc ion binding / nucleus / metal ion binding / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.35 Å | |||||||||
![]() | Li Z / Guo Q | |||||||||
![]() | ![]() Title: Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii. Authors: Zhifei Li / Qiang Guo / Lvqin Zheng / Yongsheng Ji / Yi-Ting Xie / De-Hua Lai / Zhao-Rong Lun / Xun Suo / Ning Gao / ![]() Abstract: As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High- ...As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. With cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, Trichomonas vaginalis and Toxoplasma gondii, at resolution of 3.2-3.4 Å. Although the ribosomal proteins from both pathogens are typical members of eukaryotic families, with a co-evolution pattern between certain species-specific insertions/extensions and neighboring ribosomal RNA (rRNA) expansion segments, the sizes of their rRNAs are sharply different. Very interestingly, rRNAs of T. vaginalis are in size comparable to prokaryotic counterparts, with nearly all the eukaryote-specific rRNA expansion segments missing. These structures facilitate the dissection of evolution path for ribosomal proteins and RNAs, and may aid in design of novel translation inhibitors. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 9.3 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 42.7 KB 42.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 11.3 KB | Display | ![]() |
Images | ![]() | 80.4 KB | ||
Masks | ![]() | 125 MB | ![]() | |
Filedesc metadata | ![]() | 9.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5xyiMC ![]() 6778C ![]() 6780C ![]() 6784C ![]() 5xxbC ![]() 5xxuC ![]() 5xy3C M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.32 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Small subunit of Trichomonas vaginalis ribosome
+Supramolecule #1: Small subunit of Trichomonas vaginalis ribosome
+Macromolecule #1: 18S
+Macromolecule #2: 40S ribosomal protein SA
+Macromolecule #3: Ribosomal protein S3Ae, putative
+Macromolecule #4: Uncharacterized protein
+Macromolecule #5: Ribosomal protein S3, putative
+Macromolecule #6: 40S ribosomal protein S4
+Macromolecule #7: 40s ribosomal protein S5-B, putative
+Macromolecule #8: 40S ribosomal protein S6
+Macromolecule #9: 40S ribosomal protein S7, putative
+Macromolecule #10: 40S ribosomal protein S8
+Macromolecule #11: Uncharacterized protein
+Macromolecule #12: Plectin/S10 domain containing protein
+Macromolecule #13: Uncharacterized protein
+Macromolecule #14: Ribosomal protein L7Ae, putative
+Macromolecule #15: 40S ribosomal protein S13, putative
+Macromolecule #16: Ribosomal protein S14
+Macromolecule #17: Ribosomal protein S19, putative
+Macromolecule #18: 40S ribosomal protein S16, putative
+Macromolecule #19: 40S ribosomal protein S17-B, putative
+Macromolecule #20: Ribosomal protein S13p/S18e, putative
+Macromolecule #21: Ribosomal protein S19e, putative
+Macromolecule #22: Ribosomal protein S10p/S20e, putative
+Macromolecule #23: 40S ribosomal protein S21
+Macromolecule #24: Ribosomal protein S15a
+Macromolecule #25: 40S ribosomal protein S23, putative
+Macromolecule #26: Ribosomal protein S24e, putative
+Macromolecule #27: Uncharacterized protein
+Macromolecule #28: 40S ribosomal protein S26
+Macromolecule #29: 40S ribosomal protein S27
+Macromolecule #30: Uncharacterized protein
+Macromolecule #31: 40S ribosomal protein S29, putative
+Macromolecule #32: 40S ribosomal protein S30
+Macromolecule #33: Guanine nucleotide-binding protein beta subunit, putative
+Macromolecule #34: eL41
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 2.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |