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- EMDB-6784: Large subunit of Trichomonas vaginalis ribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-6784
TitleLarge subunit of Trichomonas vaginalis ribosome
Map data
SampleLarge subunit of Trichomonas vaginalis ribosome:
(nucleic-acidNucleic acid) x 3 / (Ribosomal protein ...) x 23 / (Uncharacterized ...) x 3 / (Ribosomal protein, ...) x 2 / (60S ribosomal protein ...) x 11 / Ubiquitin, putative
Function / homology
Function and homology information


maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of LSU-rRNA / assembly of large subunit precursor of preribosome / modification-dependent protein catabolic process / cytoplasmic translation / large ribosomal subunit rRNA binding / protein tag / ribosome biogenesis / ribosomal large subunit assembly ...maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of LSU-rRNA / assembly of large subunit precursor of preribosome / modification-dependent protein catabolic process / cytoplasmic translation / large ribosomal subunit rRNA binding / protein tag / ribosome biogenesis / ribosomal large subunit assembly / cytosolic large ribosomal subunit / 5S rRNA binding / negative regulation of translation / ribosome / rRNA binding / structural constituent of ribosome / protein ubiquitination / translation / mRNA binding / ubiquitin protein ligase binding / RNA binding / metal ion binding / nucleus / cytoplasm
60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L2, domain 3 / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L18e/L15P / Ribosomal protein L14P, conserved site / Ubiquitin domain / Ubiquitin conserved site / Ribosomal protein L7Ae/L8/Nhp2 family / Ribosomal protein L37e, conserved site / Ribosomal protein L21e, conserved site ...60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L2, domain 3 / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L18e/L15P / Ribosomal protein L14P, conserved site / Ubiquitin domain / Ubiquitin conserved site / Ribosomal protein L7Ae/L8/Nhp2 family / Ribosomal protein L37e, conserved site / Ribosomal protein L21e, conserved site / Ribosomal protein L29, conserved site / Ribosomal protein L10e/L16 / Ribosomal protein L30, ferredoxin-like fold domain / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L2, domain 2 / Ribosomal protein L5 domain superfamily / Ribosomal protein L25/L23 / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L30, N-terminal / Ribosomal protein L23/L15e core domain superfamily / Nucleotide-binding alpha-beta plait domain superfamily / Nucleic acid-binding, OB-fold / Zinc-binding ribosomal protein / Ribosomal protein L37ae/L37e / Translation protein, beta-barrel domain superfamily / Translation protein SH3-like domain superfamily / Ribosomal protein L34Ae / Ribosomal protein L13 / Ribosomal protein L2, C-terminal / Ribosomal protein L30e, conserved site / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L19, eukaryotic / Ribosomal protein L21 superfamily / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal protein L13 superfamily / Ribosomal protein L14 superfamily / Ribosomal protein L6, alpha-beta domain superfamily / Ribosomal protein L22/L17 superfamily / Ribosomal protein L32e superfamily / Ribosomal L18e/L15P superfamily / Ribosomal protein L29/L35 superfamily / Ribosomal protein L19/L19e superfamily / Ribosomal protein L7, eukaryotic/archaeal / Ribosomal protein L5, N-terminal / Ribosomal protein L30, central domain, archaeal type / Ribosomal protein L5, C-terminal / Ribosomal protein L15 / Ubiquitin-like domain superfamily / 50S ribosomal protein L30e-like / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / 60S ribosomal protein L4, C-terminal domain / Ribosomal protein L5 eukaryotic/L18 archaeal, C-terminal / Ribosomal protein L15e core domain superfamily / Ribosomal protein L39e domain superfamily / Ribosomal protein L31e domain superfamily / Ribosomal protein L4 domain superfamily / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L26/L24, eukaryotic/archaeal / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L38e superfamily / Ribosomal protein L34Ae superfamily / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal protein L36e / Ribosomal protein L15e / Ribosomal protein L14P / Ribosomal protein L19/L19e / Ribosomal protein L39e / Ribosomal protein L31e / Ribosomal protein L18e / Ribosomal protein L7A/L8 superfamily / Ribosomal protein L22e superfamily / Ribosomal protein L40e superfamily / Ubiquitin-like domain / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L27e superfamily / Ribosomal protein L35A superfamily / Ribosomal protein L30/YlxQ / 60S ribosomal protein L19 / Ribosomal protein L14 / Ribosomal protein L7/L30 / Ribosomal protein L14, KOW motif / Ribosomal Protein L26/L24, KOW domain / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L3 / Ribosomal protein L44e / Ribosomal protein L6 / 60S ribosomal protein L6E / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein L7Ae conserved site / Ribosomal protein L14e domain / Ribosomal protein L38e / Ribosomal protein L37ae / Ribosomal protein L29e / Ribosomal protein L22e / Ribosomal protein L2 / Ribosomal protein L4/L1e / Ribosomal protein L5 / Ribosomal protein L40e / Ribosomal protein L7A/L8
Ribosomal protein L7Ae, putative / Ribosomal protein, putative / Ribosomal protein L29, putative / Ribosomal protein L8, putative / 60S ribosomal protein L30, putative / 60S ribosomal protein L11, putative / Ribosomal protein L15 / 60S ribosomal protein L18a / Ribosomal protein, putative / Ribosomal protein L36e, putative ...Ribosomal protein L7Ae, putative / Ribosomal protein, putative / Ribosomal protein L29, putative / Ribosomal protein L8, putative / 60S ribosomal protein L30, putative / 60S ribosomal protein L11, putative / Ribosomal protein L15 / 60S ribosomal protein L18a / Ribosomal protein, putative / Ribosomal protein L36e, putative / Ribosomal protein L32, putative / Ribosomal protein L21e, putative / Ribosomal protein L30p/L7e, putative / Ribosomal protein L37 / Ribosomal protein L38e, putative / Ribosomal protein L13, putative / Ribosomal protein L34e, putative / 60S ribosomal protein L6, putative / Ribosomal protein L14, putative / Ribosomal protein L22, putative / Ribosomal protein L23, putative / Uncharacterized protein / 60S ribosomal protein L18, putative / 60S ribosomal protein L19-3, putative / 60S ribosomal protein L39, putative / Ribosomal protein L31e, putative / 60S ribosomal protein L44, putative / Uncharacterized protein / Ribosomal protein L6, putative / Ubiquitin, putative / Ribosomal protein L35Ae, putative / Ribosomal_L18e/L15P domain-containing protein / Ribosomal protein L24e, putative / Ribosomal protein L24, putative / 60S ribosomal protein L29 / Ribosomal protein L27e, putative / Ribosomal protein L5 / 60S ribosomal protein L22-1, putative / 60S ribosomal protein L23, putative / Ribosomal protein L13e, putative
Biological speciesTrichomonas vaginalis (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsLi Z / Guo Q / Zheng L / Ji Y / Xie Y / Lai D / Lun Z / Suo X / Gao N
CitationJournal: Cell Res. / Year: 2017
Title: Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii.
Authors: Zhifei Li / Qiang Guo / Lvqin Zheng / Yongsheng Ji / Yi-Ting Xie / De-Hua Lai / Zhao-Rong Lun / Xun Suo / Ning Gao /
Abstract: As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High- ...As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. With cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, Trichomonas vaginalis and Toxoplasma gondii, at resolution of 3.2-3.4 Å. Although the ribosomal proteins from both pathogens are typical members of eukaryotic families, with a co-evolution pattern between certain species-specific insertions/extensions and neighboring ribosomal RNA (rRNA) expansion segments, the sizes of their rRNAs are sharply different. Very interestingly, rRNAs of T. vaginalis are in size comparable to prokaryotic counterparts, with nearly all the eukaryote-specific rRNA expansion segments missing. These structures facilitate the dissection of evolution path for ribosomal proteins and RNAs, and may aid in design of novel translation inhibitors.
Validation ReportPDB-ID: 5xy3

SummaryFull reportAbout validation report
History
DepositionJul 6, 2017-
Header (metadata) releaseAug 30, 2017-
Map releaseAug 30, 2017-
UpdateDec 18, 2019-
Current statusDec 18, 2019Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.044
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.044
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: : PDB-5xy3
  • Surface level: 0.044
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_6784.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.32 Å/pix.
x 320 pix.
= 422.4 Å
1.32 Å/pix.
x 320 pix.
= 422.4 Å
1.32 Å/pix.
x 320 pix.
= 422.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.32 Å
Density
Contour LevelBy AUTHOR: 0.044 / Movie #1: 0.044
Minimum - Maximum-0.23113365 - 0.39924592
Average (Standard dev.)0.0004943846 (±0.01008034)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 422.40002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.321.321.32
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z422.400422.400422.400
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.2310.3990.000

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Supplemental data

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Segmentation: #1

Fileemd_6784_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire Large subunit of Trichomonas vaginalis ribosome

EntireName: Large subunit of Trichomonas vaginalis ribosome / Number of components: 44

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Component #1: protein, Large subunit of Trichomonas vaginalis ribosome

ProteinName: Large subunit of Trichomonas vaginalis ribosome / Recombinant expression: No
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #2: nucleic-acid, 25S ribosomal RNA

nucleic acidName: 25S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: CGUAGUCUGC CAUACGCAGG AAGACCCGCU GAACUGAAGC AUCUCAUUAA GCGGAGGAAA AGAAACUAAC UAGGAUUCCC UUAGUAAAGG CGAUCGAAAA GGGAACAGCC CAACGUCUUA AUCCCGCAAG GGAAAUGUAG UCGGUUCACC UAUUGCACUG GUCUAAGUCA ...Sequence:
CGUAGUCUGC CAUACGCAGG AAGACCCGCU GAACUGAAGC AUCUCAUUAA GCGGAGGAAA AGAAACUAAC UAGGAUUCCC UUAGUAAAGG CGAUCGAAAA GGGAACAGCC CAACGUCUUA AUCCCGCAAG GGAAAUGUAG UCGGUUCACC UAUUGCACUG GUCUAAGUCA GAUGGAAUGC UGCGCGAUUC AGAGUAAAAG CCUCGUAUGA CCAACCGCAA GCUUACGAUG GACCGGGGAG AGUCGGAUAC UUUGAGAGUA GUGUCUUAAG GGGGUGGUAU GUCCCAUCCA AGGCUAAAUA ACGUGAGGAG ACCGAUAGAU UACAAGUAGU GUGAACGAAA GAUGAAAAGA ACUCUAAAAC AGAGGGUGAA AAGUUCCUGA ACUUGCUUAG GGUUAAGGCU AUUUAUAGUA CCCGUCUUGA AACACGGAUU AGGGAGUUUA UCUGUAGUGC GAGUCUUUGC ACGUUAACUG UAAAGGCGUA GAGAAAGCAA CUGGCGUUUG CAUGGCUACU GAGCAUUACU GAUAGGACCC GAAAGGUGGU GAACUUUGCC UGCACAGGUU GAAGUCAGGG GAAACUCUGA UGGAGGACCG UACGGGUUAU UGAUGUGCAA AUCAUUUUUC UGAUGUAGGU AAAGGUGCGA AAGACUCAUC GAACCACCUA AUAGCUGGUU UCCUCCGAAG UUUCCCUCAG GAUAGCUGUA AUGCAUUUAG UUAAGGGGAG UAGAGAUAUG GGUAGAGGUG CAGGGAGCCU UCGGGUUCUC AACUUAUUUC CAAACUUCGA ACCUCCUUGU UAGGCAUUUA CCUAACUGGA AAUGCUUAAA UGAAUUAGCG UUACAAGUGG GCUCUGACCG GUAAGCGGUC AUGGCGAAAU GGGAUGAACC AUACGUCUGA AUAAGGUGCC GAAAUGUACU AUCACUGAUA CCAUAAAGGG AGUCCAUGUA UUUAGACAGC AGGUCGGUGC CCCUAAAAGC AGGUAUCCGC UAAAGAGUGU GUUAAAACUC ACCUGCCGAA UACUAGGGCC CCGAAAAUGA AUGGCGCAUA UAGUACUACC GAUCUCAGAC AUCAGUUCUA UUGAACUGAG AGUAGGGGGA UGUGGACAUU UUUGUGAAGG UGCAAUGUGA GUUGGACUGG AAGAAUGUCU AUUACAGAUC CUAAUUGAAG UAGCGAUUAU CCAGGCUUGU AUCCUGGGGG CUGAUGAGGA GUAGGGUUUC UGGACAACUU AAACAGAUCC AGAGUUAGUC GAUCCUAAUC UCAAUAAUGG GAGAAAGGGA GUACAGUUGA UAUUCUGUAA CUGACAACAG UUCGCGGCAA CGCAUACUAU UUUUCUCCGC CAAUCUUAUA CCGUUGGUAG GGAUCUCUUU CCUACAUGAC AGUACUAAUA CCCAGGGAAA CUGGUCAUCA GGCGAACUGC GGCGUUCUGC UGGCAAAACA UAGCUUCGGU UAUGUCAAUG GCUAUUGGAU UGGUCCAUGA AGCGAAAAAU AGUUUACUGU GUCACUCGUA CCGAUAACCG CAUCAGGUCU UCUAGGUAAG UAGCCUAUGG CCAUUGAGGC AUGAUAGUUC AGGGAAUUCG GCCAAUUAGA GCUGUAACUU CGGGAUAAAG CUUGGCUCUG AUGAAAAUCA GAACCGAUUG UUACGCGGAG AGCCCAACUG UUUAAUAAAA ACGUAGUGUC GCGCUGUGGU AGUAUAGCCA GUGAACGCGA CAUGAAUCCU GCUCAGUGCU GUGUCUGUUA AUAUGGUUCA AGCCAACGAA GCACCGGUAA AUAGCUGGGG UAACUAUGAC UCUUAUCAAA GUAGCGAAAU ACCUCGUCAC UUAAUUAGUG ACCUGCAUGA ACGGAGCUAU GAGGUUCUCA CUGUCCCAAA CUGUCUCUCA GCGAACCAAC UGUAGUGGGA ACGGUCACUA GAUAUCCAGC GGGGAAAGAA GACCCUGUAG AGCUUCACUU UGGUCUAGUC UUGUAUAGUU CCUGCGGGGG UGUAGACUAG GUGGGAGAGC AAUCGAUUCU GAAAAACCAC UACCCUGUUU GGAAUUAUGC UUACCUAUCA CAAGGAAUAG GACUAGAUAG AAAGUUUGGC UGGGGCGGCA CACCUUCGAA AGAGCAGCGA AGGUGUCCUA AGUUGAGCUC AGUGAGGACA GAAAUCUCAC GUUGAGGAUA AGGCCAAAAG CUCAAUUUAC UGUGCCUUUU UGUACUAGGA UCAGUUACGA AAGUAAGGCC UAACGAUCCC UAAAUAGCCU CAGGUGCGUG AGGAUUGGGG UGACAGACAA GUUACCUCCG GGACAACUGG CUUGUGGCUG CUAAGAGAUC AAAUCAACGC AGCUUUUUGA UUCUUCGAUG UCGGCUCUUC CUAUCCUAGU GAUGUACAAG UUGCUAAGGG UCGGAUUGUU CACCCGUAUU AGGGAUCGUG AGCUGGGUUU AGACCGUCGU GAGCCAGGUU UGUUUUAUCC UACUGGAUAU AGCAUCAUGA UAGUAGAUUG AUUUAGUACG AGAGGAACUG UUGAUUAUGG CCUCUGGUUA GCGAUUAUCG GAAACGGUAU GUCGCGCAGC UACGCCGUCU GGGAUAAUGG CUGAAAGCCU CUUAAGCCAG AAGCCCAUCU AGAAAGUGAU GCACGUGAUG UUGGACCGAU ACGAAUAAUC AUACUCAAAA GGUCCGCUUC AUAGGUAACC CAAUGUAGAA GACAUUGUGU GAACGAAGGU ACUGUAAGCA GUGGAGUGGC AUUACGUCCA CGACCUGUUG AGGUUCAGCU UCGCUUCUAG GUUUCCAAAU AUUUU
MassTheoretical: 892.223312 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #3: nucleic-acid, 5S ribosomal RNA

nucleic acidName: 5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GAAGCGGCCA CACCCGGCUG GCAACAUCCA GCUCCACUCC GAACCUGGAA AUUAAGCAGU CGCGGGCCAC AUCAGUACUG GGCUGGGCGA CUUCCCGGGA ACGUGCGGUG CUUGCUUC
MassTheoretical: 37.963594 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #4: nucleic-acid, 5.8S ribosomal RNA

nucleic acidName: 5.8S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AAGUCUCUAA GCAAUGGAUG UCUUGGCUCC UCACACGAUG AAGAACGUGG CAUAAUGUGU UAAGUAACCG GAGUUGCAAA CAUCAUGACA GGUUAAUCUU UGAAUGCAAA UUGCGCUAAA CUCGAUCUCG GUCGAGAAGC AUGGGUGUGA CAGUACUACA UC
MassTheoretical: 52.173945 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #5: protein, Ribosomal protein L8, putative

ProteinName: Ribosomal protein L8, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.949082 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #6: protein, Uncharacterized protein

ProteinName: Uncharacterized protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 46.90427 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #7: protein, Ribosomal protein, putative

ProteinName: Ribosomal protein, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 40.320738 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #8: protein, Ribosomal protein L5

ProteinName: Ribosomal protein L560S ribosomal protein L5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.932766 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #9: protein, 60S ribosomal protein L6, putative

ProteinName: 60S ribosomal protein L6, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.045772 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #10: protein, Ribosomal protein L30p/L7e, putative

ProteinName: Ribosomal protein L30p/L7e, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.842281 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #11: protein, Ribosomal protein L7Ae, putative

ProteinName: Ribosomal protein L7Ae, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.602273 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #12: protein, Ribosomal protein L6, putative

ProteinName: Ribosomal protein L6, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.930506 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #13: protein, Ribosomal protein, putative

ProteinName: Ribosomal protein, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.800781 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #14: protein, 60S ribosomal protein L11, putative

ProteinName: 60S ribosomal protein L11, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.373488 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #15: protein, Ribosomal protein L13e, putative

ProteinName: Ribosomal protein L13e, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.865705 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #16: protein, Ribosomal protein L14, putative

ProteinName: Ribosomal protein L14, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.726112 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #17: protein, Ribosomal protein L15

ProteinName: Ribosomal protein L15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.323465 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #18: protein, Ribosomal protein L13, putative

ProteinName: Ribosomal protein L13, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.569111 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #19: protein, Ribosomal protein L22, putative

ProteinName: Ribosomal protein L22, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.53049 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #20: protein, 60S ribosomal protein L18, putative

ProteinName: 60S ribosomal protein L18, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.122545 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #21: protein, 60S ribosomal protein L19-3, putative

ProteinName: 60S ribosomal protein L19-3, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.056785 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #22: protein, 60S ribosomal protein L18a

ProteinName: 60S ribosomal protein L18a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.853973 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #23: protein, Ribosomal protein L21e, putative

ProteinName: Ribosomal protein L21e, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.910867 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #24: protein, 60S ribosomal protein L22-1, putative

ProteinName: 60S ribosomal protein L22-1, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.237054 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #25: protein, 60S ribosomal protein L23, putative

ProteinName: 60S ribosomal protein L23, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.840554 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #26: protein, Ribosomal protein L24e, putative

ProteinName: Ribosomal protein L24e, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.282475 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #27: protein, Ribosomal protein L23, putative

ProteinName: Ribosomal protein L23, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.582249 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #28: protein, Ribosomal protein L24, putative

ProteinName: Ribosomal protein L24, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.782617 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #29: protein, Ribosomal protein L27e, putative

ProteinName: Ribosomal protein L27e, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.946061 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #30: protein, Uncharacterized protein

ProteinName: Uncharacterized protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.006787 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #31: protein, 60S ribosomal protein L29

ProteinName: 60S ribosomal protein L29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.823724 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #32: protein, 60S ribosomal protein L30, putative

ProteinName: 60S ribosomal protein L30, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.316229 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #33: protein, Ribosomal protein L31e, putative

ProteinName: Ribosomal protein L31e, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.446528 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #34: protein, Ribosomal protein L32, putative

ProteinName: Ribosomal protein L32, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.249669 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #35: protein, Ribosomal protein L35Ae, putative

ProteinName: Ribosomal protein L35Ae, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.668678 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #36: protein, Ribosomal protein L34e, putative

ProteinName: Ribosomal protein L34e, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.346646 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #37: protein, Ribosomal protein L29, putative

ProteinName: Ribosomal protein L29, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.300049 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #38: protein, Ribosomal protein L36e, putative

ProteinName: Ribosomal protein L36e, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.941717 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #39: protein, Ribosomal protein L37

ProteinName: Ribosomal protein L37 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.355945 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #40: protein, Ribosomal protein L38e, putative

ProteinName: Ribosomal protein L38e, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.46896 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #41: protein, 60S ribosomal protein L39, putative

ProteinName: 60S ribosomal protein L39, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.297775 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #42: protein, Ubiquitin, putative

ProteinName: Ubiquitin, putative / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.101515 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #43: protein, 60S ribosomal protein L44, putative

ProteinName: 60S ribosomal protein L44, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.471015 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Component #44: protein, Uncharacterized protein

ProteinName: Uncharacterized protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.051994 kDa
SourceSpecies: Trichomonas vaginalis (eukaryote)

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Experimental details

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Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 2 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 57162
3D reconstructionResolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution estimation)

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Atomic model buiding

Output model

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