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- EMDB-6778: Large subunit of Toxoplasma gondii ribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-6778
TitleLarge subunit of Toxoplasma gondii ribosome
Map data
SampleLarge subunit of Toxoplasma gondii ribosome:
(nucleic-acidNucleic acid) x 3 / (Ribosomal protein ...) x 41
Biological speciesToxoplasma gondii (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.17 Å
AuthorsLi Z / Guo Q / Zheng L / Ji Y / Xie Y / Lai D / Lun Z / Suo X / Gao N
CitationJournal: Cell Res. / Year: 2017
Title: Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii.
Authors: Zhifei Li / Qiang Guo / Lvqin Zheng / Yongsheng Ji / Yi-Ting Xie / De-Hua Lai / Zhao-Rong Lun / Xun Suo / Ning Gao /
Abstract: As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High- ...As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. With cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, Trichomonas vaginalis and Toxoplasma gondii, at resolution of 3.2-3.4 Å. Although the ribosomal proteins from both pathogens are typical members of eukaryotic families, with a co-evolution pattern between certain species-specific insertions/extensions and neighboring ribosomal RNA (rRNA) expansion segments, the sizes of their rRNAs are sharply different. Very interestingly, rRNAs of T. vaginalis are in size comparable to prokaryotic counterparts, with nearly all the eukaryote-specific rRNA expansion segments missing. These structures facilitate the dissection of evolution path for ribosomal proteins and RNAs, and may aid in design of novel translation inhibitors.
Validation ReportPDB-ID: 5xxb

SummaryFull reportAbout validation report
History
DepositionJul 3, 2017-
Header (metadata) releaseAug 30, 2017-
Map releaseAug 30, 2017-
UpdateOct 18, 2017-
Current statusOct 18, 2017Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: : PDB-5xxb
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_6778.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.32 Å/pix.
x 320 pix.
= 422.4 Å
1.32 Å/pix.
x 320 pix.
= 422.4 Å
1.32 Å/pix.
x 320 pix.
= 422.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.32 Å
Density
Contour LevelBy AUTHOR: 0.04 / Movie #1: 0.04
Minimum - Maximum-0.21132639 - 0.35448036
Average (Standard dev.)0.0006059298 (±0.009347305)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 422.40002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.321.321.32
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z422.400422.400422.400
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.2110.3540.001

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Supplemental data

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Segmentation: #1

Fileemd_6778_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire Large subunit of Toxoplasma gondii ribosome

EntireName: Large subunit of Toxoplasma gondii ribosome / Number of components: 45

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Component #1: protein, Large subunit of Toxoplasma gondii ribosome

ProteinName: Large subunit of Toxoplasma gondii ribosome / Recombinant expression: No
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #2: nucleic-acid, 25S RNA

nucleic acidName: 25S RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: AUCAGACCUG AAAUCAGGCG AGGUUACCCG CUGAACUUAA GCAUAUUAUU AAGCGGAGGA GAAGAAAAUA ACUAUGAUUC CCUUAGUAAC GGCGAGUGAA CCGGGAUCAG CUCAAAGUGG AAAUCAACUG CUCUUUCCGA GCUGUUGACU UGUAGCCUCG AGAGGCGUUA ...Sequence:
AUCAGACCUG AAAUCAGGCG AGGUUACCCG CUGAACUUAA GCAUAUUAUU AAGCGGAGGA GAAGAAAAUA ACUAUGAUUC CCUUAGUAAC GGCGAGUGAA CCGGGAUCAG CUCAAAGUGG AAAUCAACUG CUCUUUCCGA GCUGUUGACU UGUAGCCUCG AGAGGCGUUA CCAGUGGAAG CGCAGGGUUA AGUUUCUUGG GAAAGAACAU CAUAGAGGGU GAGAAUCCCG UCCUUCCCCU GCGUUUUUUG UUCCACGUAC GGUACGCUUU UAUAGAGUCG CGUUCAUCGG GAUUUGAGCG CAAAAUGUGU GGUAGGUUUC ACAUAAAGCU AAGUACUGGC GCGAGACCGA UAGCAAACAA GUACCGUGAG GGAAAGAUGA AAAGUUCUUU GGAAAGAGAG UUAAAAGUGC CUGAAAUUGC UGAAAAGGAA GCGAUUGGGA CCUUUUUGAU UCCAAGAAGU GCCGCCGGUU UGCCAUCUAU CCCUUUGCCU UCUCUCUCUC ACCAGGUUUA GGCUUUGGUU UCUUGAUGUA UUUCCGGUUC AUCUUUUUGG AGAGGUCAGC AUCAGCUACU CGGAAUUGAU UUCGUUCAAA GAAGGUAGGU UCCUUGUGGA CCGUUAUAUC UUUGUUCUUU CCUUUUCCUU GUGGCUGAGG AGUGUUCCUG UUUCCGAGCU CCACUUUCGA GUACUCGGUU UCUGUGAUGC UGGCUUAAUC GGUUCCAACC GACCCGUCUU GAAACACGGA CCAAGGAGUC UAACAUAUGU GCGAGUAUGC GGGUUUUACU CCUGUAUGCG CAAUGAAAGU GAGAGUAGGG AGAUUUUGCU UUGCCAUUCU UCGCACCUAC GACCGACCAC GAUCUCUGAG AGAGGUUUGA GUUGGAGCAU AUCUGUUAGG ACCCGAAAGA UGGUGAACUA UGCCUGAGUA GAAUGAAGUC AGGGGAAACC CUGAUGGAGG UUCGUAGCGA UACUGACGUG CAAAUCGUUC GUCAAACUUG GGUAUAGGGG CGAAAGACUA AUCGAACCAU CUAGUAGCUG GUUUCUUCCG AAGUUUCUCU CAGGAUAGCU GGAGUUUUGU UAGUUUUAUC AGGUAAAGCG AAUGAUUAGA GGCAUCGGGG GCGCGUCGCC CUCGACCUAU UCUCAAACUU UAAAUGGGUA AGAUCCAGUG GUUACUUCAU UGAACUGCUG GGAUUAAUAU GAACUCCAAG UGGGCCAUUU UUGGUAAGCA GAACUGGCGA UGAGGGAUGC UCCUAACGCU UGGUUAAGGU GCCAAAAUGC UCGCUCAUCA GAUACCAUAA AAGGUGUUGG UUCAUUUUGA CAGCAGGACG GUGGUCAUGG AAGUCGAAAU CCGCUAAGGA GUGUGUAACA ACUCACCUGC CGAAUGAACC AGCCCUGAAA AUGGAUGGCG CUGAAGCGGG CUACCGAUAC CAGGCCAUUG CUACUAAUUA UCACUCGACC UUUUGUAGCA AUGAGUAGGA AGACGUGGGG AUUGUGACGA AGCGUUCGGC GUAAGCCUGC GUGGAACAGU CUCUAGUGCA GAUCUUGGUG GUAGUAGCAA AUAUUCAAAU GAGAACUUUG AAGACUGAAG UGGAGAAGGG UUCCAUGUGA ACAGCAAUUG GACAUGGGUU AGCCGGUCCU AAGCGACAGC UUAACUGCGU AUACGUUAGG UUUCUUUACC UCGUUUCGCG AAAGGGAAUC GGGUUAAUAU UCCCGAGCCA GGACGUGGAU ACUGUACGGU AACGUAAGUG AACUCCUCGA CGCAGGCAGG UGCUCCGGGA AGCGUUGUCU UUGCUUUUUA ACAGCCUUUU CUUUUAUACC CUGGAAUCAG UUUAGCUGGA GAUAGGGUUA GCUGGCUGGU AGAGCACCUU GGUUUCCAAG GUGUCCGGUG CGCUCCUGAC UGCCCUUGAA AAGAGGAGGG AAAGUUGUUA UUUACACCCC UGGCCGUACC AAUAACCGCA UCAGGUCUCC AAGGUUAGUA GCCUCUGGUC GAUAGAAUAA GGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGUAACUUCG GGAAAAGGAU UGGCUCUGAG GGCUGGGUAC AUUGCGCCCC AGUGGCCUGU AUUUGACGGA GCUUGGGACU GCUUCAUGCU UCGCGGCGUG UUGCGGACCC GAGCACUGUC UGUAUUGCGC UCUGGGGAAA AGGUCCAUCU CCCUUUCCGG GGUCUUGGGC UGUGCGUGUA CGGUGAACAG CUAACUCAGA ACUGGAGCGG ACAAGGGGAA UCCGACUGUU UAAUUAAAAC AAAGCAUUGC GAUGGCCAUA ACUGGUGUUG ACGCAAUGUG AUUUCUGCCC AGUGCUCUGA AUGUCAAUGU GAUGAAAUUC AAUCAAGCGC GGGUAAACGG CGGGAGUAAC UAUGACUCUC UUAAGGUAGC CAAAUGCCUC GUCAUCUAAU UAGUGACGCG CAUGAAUGGA UUAACGAGAU UCCCACUGUC CCUAUCUACU AUCUAGCGAA ACCACAGCCA AGGGAACGGG CUUGGCAGAA UCAGCGGGGA AAGAAGACCC UGUUGAGCUU GACUCUAGUC CGACUUUGUG AAAUGACUUA GGAGGUGUAG CAUAAGUGGG AGCUUCGGCG CUCGUGAAAU ACCACUACUC UUAAUGUCGU UUUACUUAUU CCGUUAAGUU GAGACUCAUG CUCUGCAUGU GUUUUGGUUU UAAGGUGGCU UUUGCCGCUG AUCUACGCGG AAGACACAGU CAGGUUGGGA GUUUGGCUGG GGCGGCACAU CUGUGAAACA AUAGCGCAGG UGUCCUAAGG CAAGCUCAAU GAGAACAGAA AUCUCAUGUU GACCAAAAGG GGAAAAGCUU GCUUGAUUUU GAUUUUCAGU ACGAAUACAA ACUGUGAAAG CAUGGCCUAU CGAUCCUUUA GGUCUGAGAA ACUUUCAGCU AGAGGUGUCA GAAAAGUUAC CACAGGGAUA ACUGGCUUGU GGCAGCCAAG CGUUCAUAGC GACGUUGCUU UUUGAUCCUU CGAUGUCGGC UCUUCCUAUC AUUGAGACGC AGAAGUCUCA AAGUGUCGGA UUGUUCACCC GCCAAUAGGG AACGUGAGCU GGGUUUAGAC CGUCGUGAGA CAGGUUAGUU UUACCCUACU GAUGACUUGA UACUGCGACA GUAAUCCAAC UUAGUACGAG AGGAACAGUU GGGUCGGAUA CUUGGUAAAU GCGGCUAGCU GAAAAGCUAG UGCCGCGACG CUACCAUCCG UUGGAUUAUG ACUGAACGCC UCUAAGUCAG AAUCCAUGCU GGAUAUGCAG UGUUAUUCAC CGCCUCCUUU GUUUCAUGCA AUGAUACAGG CCUCUGGCCU GCUUCUCAUC AAAGCCCUCC GCUUCAUGCU UUUGGGCGAG AAUCUCAAUU UCUAACAUUG GAGGUGAUAC AUCCAUUGCA GACGACUUUG UUGCAACCGG GUAUUGUAAG UAUGAGAGUA GGCUUGUCUA CGAUCUACUA AGAUUUUUCC UGCGUUGUUC GAUGUGG
MassTheoretical: 1.120153125 MDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #3: nucleic-acid, 5S RNA

nucleic acidName: 5S RNA5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GCUGUCGGUC AUACUGCGUC GAAUACACCG GAUCCCUUCA GACCUCCGAA GUUAAGCGGC GCAAGGCCCG GUUAGUACUU GGGUGGGGGA CUCCCAGGGA AUACCCUCGG GUGCUGACAG CUUU
MassTheoretical: 39.921641 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #4: nucleic-acid, 5.8S RNA

nucleic acidName: 5.8S RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AAAUUUUCAG CAAUGGAUGU CUUGGCUCGC GCAACGAUGA AGGACGCAGC GAACUGCGAA ACGCAAUGUG AAUUGCAGAA UUCAGUGAAU CAUCAGAUUU CUGAACGCAA AUGGCACCUU GGGGAUAUUC UCCUUGGUAC GUCUGUUUCA GUGUCUUU
MassTheoretical: 50.78798 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #5: protein, Ribosomal protein uL2

ProteinName: Ribosomal protein uL2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.314898 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #6: protein, Ribosomal protein uL3

ProteinName: Ribosomal protein uL3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 44.150738 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #7: protein, Ribosomal protein uL4

ProteinName: Ribosomal protein uL4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 45.837258 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #8: protein, Ribosomal protein uL18

ProteinName: Ribosomal protein uL18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.408742 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #9: protein, Ribosomal protein eL6

ProteinName: Ribosomal protein eL6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.563535 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #10: protein, Ribosomal protein uL30

ProteinName: Ribosomal protein uL30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 30.187777 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #11: protein, Ribosomal protein eL8

ProteinName: Ribosomal protein eL8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 31.095934 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #12: protein, Ribosomal protein uL6

ProteinName: Ribosomal protein uL6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.459178 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #13: protein, Ribosomal protein uL16

ProteinName: Ribosomal protein uL16 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.846014 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #14: protein, Ribosomal protein uL5

ProteinName: Ribosomal protein uL5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.240361 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #15: protein, Ribosomal protein eL13

ProteinName: Ribosomal protein eL13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 39.757371 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #16: protein, Ribosomal protein eL14

ProteinName: Ribosomal protein eL14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.492432 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #17: protein, Ribosomal protein eL15

ProteinName: Ribosomal protein eL15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.387846 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #18: protein, Ribosomal protein uL13

ProteinName: Ribosomal protein uL13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 31.757688 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #19: protein, Ribosomal protein uL22

ProteinName: Ribosomal protein uL22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.319188 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #20: protein, Ribosomal protein eL18

ProteinName: Ribosomal protein eL18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.223158 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #21: protein, Ribosomal protein eL19

ProteinName: Ribosomal protein eL19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.221574 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #22: protein, Ribosomal protein eL20

ProteinName: Ribosomal protein eL20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.372252 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #23: protein, Ribosomal protein eL21

ProteinName: Ribosomal protein eL21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.932977 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #24: protein, Ribosomal protein eL22

ProteinName: Ribosomal protein eL22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.103659 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #25: protein, Ribosomal protein uL14

ProteinName: Ribosomal protein uL14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.842553 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #26: protein, Ribosomal protein eL24

ProteinName: Ribosomal protein eL24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.644857 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #27: protein, Ribosomal protein uL23

ProteinName: Ribosomal protein uL23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.600809 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #28: protein, Ribosomal protein uL24

ProteinName: Ribosomal protein uL24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.353228 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #29: protein, Ribosomal protein eL27

ProteinName: Ribosomal protein eL27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.841355 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #30: protein, Ribosomal protein uL15

ProteinName: Ribosomal protein uL15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.384355 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #31: protein, Ribosomal protein eL29

ProteinName: Ribosomal protein eL29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.387679 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #32: protein, Ribosomal protein eL30

ProteinName: Ribosomal protein eL30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.682658 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #33: protein, Ribosomal protein eL31

ProteinName: Ribosomal protein eL31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.389729 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #34: protein, Ribosomal protein eL32

ProteinName: Ribosomal protein eL32 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.652334 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #35: protein, Ribosomal protein eL33

ProteinName: Ribosomal protein eL33 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.873091 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #36: protein, Ribosomal protein eL34

ProteinName: Ribosomal protein eL34 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.427309 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #37: protein, Ribosomal protein uL29

ProteinName: Ribosomal protein uL29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.231108 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #38: protein, Ribosomal protein eL36

ProteinName: Ribosomal protein eL36 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.437687 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #39: protein, Ribosomal protein eL37

ProteinName: Ribosomal protein eL37 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.25621 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #40: protein, Ribosomal protein eL38

ProteinName: Ribosomal protein eL38 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.606386 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #41: protein, Ribosomal protein eL39

ProteinName: Ribosomal protein eL39 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.172519 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #42: protein, Ribosomal protein eL40

ProteinName: Ribosomal protein eL40 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.714291 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #43: protein, Ribosomal protein eL42

ProteinName: Ribosomal protein eL42 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.098689 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #44: protein, Ribosomal protein eL43

ProteinName: Ribosomal protein eL43 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.426384 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Component #45: protein, Ribosomal protein eL28

ProteinName: Ribosomal protein eL28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.787717 kDa
SourceSpecies: Toxoplasma gondii (eukaryote)

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Experimental details

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Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

ImagingMicroscope: FEI TITAN
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 2 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 108162
3D reconstructionResolution: 3.17 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution estimation)

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Atomic model buiding

Output model

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