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- EMDB-6778: Large subunit of Toxoplasma gondii ribosome -

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Basic information

Entry
Database: EMDB / ID: 6778
TitleLarge subunit of Toxoplasma gondii ribosome
SampleLarge subunit of Toxoplasma gondii ribosome
SourceToxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /
Map data
Methodsingle particle reconstruction, at 3.17 Å resolution
AuthorsLi Z / Guo Q
CitationCell Res., 2017

Cell Res., 2017 StrPapers
Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii.
Zhifei Li / Qiang Guo / Lvqin Zheng / Yongsheng Ji / Yi-Ting Xie / De-Hua Lai / Zhao-Rong Lun / Xun Suo / Ning Gao

Validation ReportPDB-ID: 5xxb

SummaryFull reportAbout validation report
DateDeposition: Jul 3, 2017 / Header (metadata) release: Aug 30, 2017 / Map release: Aug 30, 2017 / Last update: Aug 30, 2017

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.04
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by height
  • Surface level: 0.04
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view with fitted model
  • Atomic models: : PDB-5xxb
  • Surface level: 0.04
  • Imaged by UCSF CHIMERA
  • Download
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Supplemental images

Downloads & links

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Map

Fileemd_6778.map.gz (map file in CCP4 format, 131073 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
320 pix
1.32 Å/pix.
= 422.4 Å
320 pix
1.32 Å/pix.
= 422.4 Å
320 pix
1.32 Å/pix.
= 422.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 1.32 Å
Density
Contour Level:0.04 (by author), 0.04 (movie #1):
Minimum - Maximum-0.21132639 - 0.35448036
Average (Standard dev.)0.0006059298 (0.009347305)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions320320320
Origin000
Limit319319319
Spacing320320320
CellA=B=C: 422.40002 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.321.321.32
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z422.400422.400422.400
α/β/γ90.00090.00090.000
start NX/NY/NZ-190-190-190
NX/NY/NZ380380380
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.2110.3540.001

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Supplemental data

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Mask #1

Fileemd_6778_msk_1.map ( map file in CCP4 format, 131073 KB )
Projections & Slices
AxesZYX
Projections
Slices (1/2)
Density Histograms
Data typeImage stored as Reals
Space group number1

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Mask #1~

Fileemd_6778_msk_1.map
Projections & Slices
AxesZYX
Projections
Slices (1/2)
Density Histograms

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Sample components

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Entire Large subunit of Toxoplasma gondii ribosome

EntireName: Large subunit of Toxoplasma gondii ribosome / Number of components: 45

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Component #1: protein, Large subunit of Toxoplasma gondii ribosome

ProteinName: Large subunit of Toxoplasma gondii ribosome / Recombinant expression: No
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #2: nucleic-acid, 25S RNA

Nucleic-acidName: 25S RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AUCAGACCUG AAAUCAGGCG AGGUUACCCG CUGAACUUAA GCAUAUUAUU AAGCGGAGGA GAAGAAAAUA ACUAUGAUUC CCUUAGUAAC GGCGAGUGAA CCGGGAUCAG CUCAAAGUGG AAAUCAACUG CUCUUUCCGA GCUGUUGACU UGUAGCCUCG AGAGGCGUUA CCAGUGGAAG CGCAGGGUUA AGUUUCUUGG GAAAGAACAU CAUAGAGGGU GAGAAUCCCG UCCUUCCCCU GCGUUUUUUG UUCCACGUAC GGUACGCUUU UAUAGAGUCG CGUUCAUCGG GAUUUGAGCG CAAAAUGUGU GGUAGGUUUC ACAUAAAGCU AAGUACUGGC GCGAGACCGA UAGCAAACAA GUACCGUGAG GGAAAGAUGA AAAGUUCUUU GGAAAGAGAG UUAAAAGUGC CUGAAAUUGC UGAAAAGGAA GCGAUUGGGA CCUUUUUGAU UCCAAGAAGU GCCGCCGGUU UGCCAUCUAU CCCUUUGCCU UCUCUCUCUC ACCAGGUUUA GGCUUUGGUU UCUUGAUGUA UUUCCGGUUC AUCUUUUUGG AGAGGUCAGC AUCAGCUACU CGGAAUUGAU UUCGUUCAAA GAAGGUAGGU UCCUUGUGGA CCGUUAUAUC UUUGUUCUUU CCUUUUCCUU GUGGCUGAGG AGUGUUCCUG UUUCCGAGCU CCACUUUCGA GUACUCGGUU UCUGUGAUGC UGGCUUAAUC GGUUCCAACC GACCCGUCUU GAAACACGGA CCAAGGAGUC UAACAUAUGU GCGAGUAUGC GGGUUUUACU CCUGUAUGCG CAAUGAAAGU GAGAGUAGGG AGAUUUUGCU UUGCCAUUCU UCGCACCUAC GACCGACCAC GAUCUCUGAG AGAGGUUUGA GUUGGAGCAU AUCUGUUAGG ACCCGAAAGA UGGUGAACUA UGCCUGAGUA GAAUGAAGUC AGGGGAAACC CUGAUGGAGG UUCGUAGCGA UACUGACGUG CAAAUCGUUC GUCAAACUUG GGUAUAGGGG CGAAAGACUA AUCGAACCAU CUAGUAGCUG GUUUCUUCCG AAGUUUCUCU CAGGAUAGCU GGAGUUUUGU UAGUUUUAUC AGGUAAAGCG AAUGAUUAGA GGCAUCGGGG GCGCGUCGCC CUCGACCUAU UCUCAAACUU UAAAUGGGUA AGAUCCAGUG GUUACUUCAU UGAACUGCUG GGAUUAAUAU GAACUCCAAG UGGGCCAUUU UUGGUAAGCA GAACUGGCGA UGAGGGAUGC UCCUAACGCU UGGUUAAGGU GCCAAAAUGC UCGCUCAUCA GAUACCAUAA AAGGUGUUGG UUCAUUUUGA CAGCAGGACG GUGGUCAUGG AAGUCGAAAU CCGCUAAGGA GUGUGUAACA ACUCACCUGC CGAAUGAACC AGCCCUGAAA AUGGAUGGCG CUGAAGCGGG CUACCGAUAC CAGGCCAUUG CUACUAAUUA UCACUCGACC UUUUGUAGCA AUGAGUAGGA AGACGUGGGG AUUGUGACGA AGCGUUCGGC GUAAGCCUGC GUGGAACAGU CUCUAGUGCA GAUCUUGGUG GUAGUAGCAA AUAUUCAAAU GAGAACUUUG AAGACUGAAG UGGAGAAGGG UUCCAUGUGA ACAGCAAUUG GACAUGGGUU AGCCGGUCCU AAGCGACAGC UUAACUGCGU AUACGUUAGG UUUCUUUACC UCGUUUCGCG AAAGGGAAUC GGGUUAAUAU UCCCGAGCCA GGACGUGGAU ACUGUACGGU AACGUAAGUG AACUCCUCGA CGCAGGCAGG UGCUCCGGGA AGCGUUGUCU UUGCUUUUUA ACAGCCUUUU CUUUUAUACC CUGGAAUCAG UUUAGCUGGA GAUAGGGUUA GCUGGCUGGU AGAGCACCUU GGUUUCCAAG GUGUCCGGUG CGCUCCUGAC UGCCCUUGAA AAGAGGAGGG AAAGUUGUUA UUUACACCCC UGGCCGUACC AAUAACCGCA UCAGGUCUCC AAGGUUAGUA GCCUCUGGUC GAUAGAAUAA GGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGUAACUUCG GGAAAAGGAU UGGCUCUGAG GGCUGGGUAC AUUGCGCCCC AGUGGCCUGU AUUUGACGGA GCUUGGGACU GCUUCAUGCU UCGCGGCGUG UUGCGGACCC GAGCACUGUC UGUAUUGCGC UCUGGGGAAA AGGUCCAUCU CCCUUUCCGG GGUCUUGGGC UGUGCGUGUA CGGUGAACAG CUAACUCAGA ACUGGAGCGG ACAAGGGGAA UCCGACUGUU UAAUUAAAAC AAAGCAUUGC GAUGGCCAUA ACUGGUGUUG ACGCAAUGUG AUUUCUGCCC AGUGCUCUGA AUGUCAAUGU GAUGAAAUUC AAUCAAGCGC GGGUAAACGG CGGGAGUAAC UAUGACUCUC UUAAGGUAGC CAAAUGCCUC GUCAUCUAAU UAGUGACGCG CAUGAAUGGA UUAACGAGAU UCCCACUGUC CCUAUCUACU AUCUAGCGAA ACCACAGCCA AGGGAACGGG CUUGGCAGAA UCAGCGGGGA AAGAAGACCC UGUUGAGCUU GACUCUAGUC CGACUUUGUG AAAUGACUUA GGAGGUGUAG CAUAAGUGGG AGCUUCGGCG CUCGUGAAAU ACCACUACUC UUAAUGUCGU UUUACUUAUU CCGUUAAGUU GAGACUCAUG CUCUGCAUGU GUUUUGGUUU UAAGGUGGCU UUUGCCGCUG AUCUACGCGG AAGACACAGU CAGGUUGGGA GUUUGGCUGG GGCGGCACAU CUGUGAAACA AUAGCGCAGG UGUCCUAAGG CAAGCUCAAU GAGAACAGAA AUCUCAUGUU GACCAAAAGG GGAAAAGCUU GCUUGAUUUU GAUUUUCAGU ACGAAUACAA ACUGUGAAAG CAUGGCCUAU CGAUCCUUUA GGUCUGAGAA ACUUUCAGCU AGAGGUGUCA GAAAAGUUAC CACAGGGAUA ACUGGCUUGU GGCAGCCAAG CGUUCAUAGC GACGUUGCUU UUUGAUCCUU CGAUGUCGGC UCUUCCUAUC AUUGAGACGC AGAAGUCUCA AAGUGUCGGA UUGUUCACCC GCCAAUAGGG AACGUGAGCU GGGUUUAGAC CGUCGUGAGA CAGGUUAGUU UUACCCUACU GAUGACUUGA UACUGCGACA GUAAUCCAAC UUAGUACGAG AGGAACAGUU GGGUCGGAUA CUUGGUAAAU GCGGCUAGCU GAAAAGCUAG UGCCGCGACG CUACCAUCCG UUGGAUUAUG ACUGAACGCC UCUAAGUCAG AAUCCAUGCU GGAUAUGCAG UGUUAUUCAC CGCCUCCUUU GUUUCAUGCA AUGAUACAGG CCUCUGGCCU GCUUCUCAUC AAAGCCCUCC GCUUCAUGCU UUUGGGCGAG AAUCUCAAUU UCUAACAUUG GAGGUGAUAC AUCCAUUGCA GACGACUUUG UUGCAACCGG GUAUUGUAAG UAUGAGAGUA GGCUUGUCUA CGAUCUACUA AGAUUUUUCC UGCGUUGUUC GAUGUGG
MassTheoretical: 1.120153125 MDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #3: nucleic-acid, 5S RNA

Nucleic-acidName: 5S RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GCUGUCGGUC AUACUGCGUC GAAUACACCG GAUCCCUUCA GACCUCCGAA GUUAAGCGGC GCAAGGCCCG GUUAGUACUU GGGUGGGGGA CUCCCAGGGA AUACCCUCGG GUGCUGACAG CUUU
MassTheoretical: 39.921641 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #4: nucleic-acid, 5.8S RNA

Nucleic-acidName: 5.8S RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AAAUUUUCAG CAAUGGAUGU CUUGGCUCGC GCAACGAUGA AGGACGCAGC GAACUGCGAA ACGCAAUGUG AAUUGCAGAA UUCAGUGAAU CAUCAGAUUU CUGAACGCAA AUGGCACCUU GGGGAUAUUC UCCUUGGUAC GUCUGUUUCA GUGUCUUU
MassTheoretical: 50.78798 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #5: protein, Ribosomal protein uL2

ProteinName: Ribosomal protein uL2 / Recombinant expression: No
MassTheoretical: 28.314898 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #6: protein, Ribosomal protein uL3

ProteinName: Ribosomal protein uL3 / Recombinant expression: No
MassTheoretical: 44.150738 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #7: protein, Ribosomal protein uL4

ProteinName: Ribosomal protein uL4 / Recombinant expression: No
MassTheoretical: 45.837258 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #8: protein, Ribosomal protein uL18

ProteinName: Ribosomal protein uL18 / Recombinant expression: No
MassTheoretical: 35.408742 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #9: protein, Ribosomal protein eL6

ProteinName: Ribosomal protein eL6 / Recombinant expression: No
MassTheoretical: 21.563535 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #10: protein, Ribosomal protein uL30

ProteinName: Ribosomal protein uL30 / Recombinant expression: No
MassTheoretical: 30.187777 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #11: protein, Ribosomal protein eL8

ProteinName: Ribosomal protein eL8 / Recombinant expression: No
MassTheoretical: 31.095934 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #12: protein, Ribosomal protein uL6

ProteinName: Ribosomal protein uL6 / Recombinant expression: No
MassTheoretical: 21.459178 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #13: protein, Ribosomal protein uL16

ProteinName: Ribosomal protein uL16 / Recombinant expression: No
MassTheoretical: 24.846014 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #14: protein, Ribosomal protein uL5

ProteinName: Ribosomal protein uL5 / Recombinant expression: No
MassTheoretical: 20.240361 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #15: protein, Ribosomal protein eL13

ProteinName: Ribosomal protein eL13 / Recombinant expression: No
MassTheoretical: 39.757371 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #16: protein, Ribosomal protein eL14

ProteinName: Ribosomal protein eL14 / Recombinant expression: No
MassTheoretical: 15.492432 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #17: protein, Ribosomal protein eL15

ProteinName: Ribosomal protein eL15 / Recombinant expression: No
MassTheoretical: 24.387846 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #18: protein, Ribosomal protein uL13

ProteinName: Ribosomal protein uL13 / Recombinant expression: No
MassTheoretical: 31.757688 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #19: protein, Ribosomal protein uL22

ProteinName: Ribosomal protein uL22 / Recombinant expression: No
MassTheoretical: 22.319188 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #20: protein, Ribosomal protein eL18

ProteinName: Ribosomal protein eL18 / Recombinant expression: No
MassTheoretical: 21.223158 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #21: protein, Ribosomal protein eL19

ProteinName: Ribosomal protein eL19 / Recombinant expression: No
MassTheoretical: 22.221574 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #22: protein, Ribosomal protein eL20

ProteinName: Ribosomal protein eL20 / Recombinant expression: No
MassTheoretical: 21.372252 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #23: protein, Ribosomal protein eL21

ProteinName: Ribosomal protein eL21 / Recombinant expression: No
MassTheoretical: 17.932977 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #24: protein, Ribosomal protein eL22

ProteinName: Ribosomal protein eL22 / Recombinant expression: No
MassTheoretical: 15.103659 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #25: protein, Ribosomal protein uL14

ProteinName: Ribosomal protein uL14 / Recombinant expression: No
MassTheoretical: 14.842553 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #26: protein, Ribosomal protein eL24

ProteinName: Ribosomal protein eL24 / Recombinant expression: No
MassTheoretical: 17.644857 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #27: protein, Ribosomal protein uL23

ProteinName: Ribosomal protein uL23 / Recombinant expression: No
MassTheoretical: 18.600809 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #28: protein, Ribosomal protein uL24

ProteinName: Ribosomal protein uL24 / Recombinant expression: No
MassTheoretical: 16.353228 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #29: protein, Ribosomal protein eL27

ProteinName: Ribosomal protein eL27 / Recombinant expression: No
MassTheoretical: 16.841355 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #30: protein, Ribosomal protein uL15

ProteinName: Ribosomal protein uL15 / Recombinant expression: No
MassTheoretical: 16.384355 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #31: protein, Ribosomal protein eL29

ProteinName: Ribosomal protein eL29 / Recombinant expression: No
MassTheoretical: 6.387679 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #32: protein, Ribosomal protein eL30

ProteinName: Ribosomal protein eL30 / Recombinant expression: No
MassTheoretical: 11.682658 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #33: protein, Ribosomal protein eL31

ProteinName: Ribosomal protein eL31 / Recombinant expression: No
MassTheoretical: 14.389729 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #34: protein, Ribosomal protein eL32

ProteinName: Ribosomal protein eL32 / Recombinant expression: No
MassTheoretical: 15.652334 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #35: protein, Ribosomal protein eL33

ProteinName: Ribosomal protein eL33 / Recombinant expression: No
MassTheoretical: 12.873091 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #36: protein, Ribosomal protein eL34

ProteinName: Ribosomal protein eL34 / Recombinant expression: No
MassTheoretical: 15.427309 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #37: protein, Ribosomal protein uL29

ProteinName: Ribosomal protein uL29 / Recombinant expression: No
MassTheoretical: 14.231108 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #38: protein, Ribosomal protein eL36

ProteinName: Ribosomal protein eL36 / Recombinant expression: No
MassTheoretical: 11.437687 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #39: protein, Ribosomal protein eL37

ProteinName: Ribosomal protein eL37 / Recombinant expression: No
MassTheoretical: 11.25621 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #40: protein, Ribosomal protein eL38

ProteinName: Ribosomal protein eL38 / Recombinant expression: No
MassTheoretical: 9.606386 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #41: protein, Ribosomal protein eL39

ProteinName: Ribosomal protein eL39 / Recombinant expression: No
MassTheoretical: 6.172519 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #42: protein, Ribosomal protein eL40

ProteinName: Ribosomal protein eL40 / Recombinant expression: No
MassTheoretical: 14.714291 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #43: protein, Ribosomal protein eL42

ProteinName: Ribosomal protein eL42 / Recombinant expression: No
MassTheoretical: 12.098689 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #44: protein, Ribosomal protein eL43

ProteinName: Ribosomal protein eL43 / Recombinant expression: No
MassTheoretical: 10.426384 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Component #45: protein, Ribosomal protein eL28

ProteinName: Ribosomal protein eL28 / Recombinant expression: No
MassTheoretical: 14.787717 kDa
SourceSpecies: Toxoplasma gondii / eukaryote / トキソプラズマ, トクソプラズマ・ゴンディイ /

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Experimental details

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Sample preparation

Specimen stateparticle
Sample solutionpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

ImagingMicroscope: FEI TITAN
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 2 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 108162
3D reconstructionResolution: 3.17 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution assessment)

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Atomic model buiding

Output model

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  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.

Related info.: Yorodumi (legacy version) / EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Yorodumi Papers / Jmol/JSmol / Changes in new EM Navigator and Yorodumi

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