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- PDB-5umd: Structure of the Plasmodium falciparum 80S ribosome bound to the ... -

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Entry
Database: PDB / ID: 5umd
TitleStructure of the Plasmodium falciparum 80S ribosome bound to the antimalarial drug mefloquine
Components
  • (60S ribosomal protein ...) x 39
  • (Ribosomal protein ...) x 2
  • 28S ribosomal RNA
  • 5.8S ribosomal RNA
  • 5S ribosomal RNA
  • Ubiquitin-60S ribosomal protein L40
KeywordsRIBOSOME / Ribosome / protein synthesis / antimalarial
Function / homologyRibosomal protein L34Ae superfamily / Ubiquitin family / Ribosomal protein L40e superfamily / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L27e superfamily / Ribosomal protein L35A superfamily / Ribosomal protein L30/YlxQ / 60S ribosomal protein L19 / Ribosomal protein L14 / Ribosomal protein L7/L30 ...Ribosomal protein L34Ae superfamily / Ubiquitin family / Ribosomal protein L40e superfamily / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L27e superfamily / Ribosomal protein L35A superfamily / Ribosomal protein L30/YlxQ / 60S ribosomal protein L19 / Ribosomal protein L14 / Ribosomal protein L7/L30 / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L22p/L17e / Ribosomal protein L14p/L23e / Ribosomal protein L16p/L10e / Ribosomal protein L7A/L8 superfamily / Ribosomal protein L23 / Ribosomal protein L5 / Ribosomal protein L3 / Ribosomal protein L30p/L7e / Ribosomal protein L6 / KOW motif / Ribosomal protein L13 / Ribosomal protein L4/L1 family / ribosomal L5P family C-terminus / Ribosomal L15 / Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A / Ribosomal protein L22e superfamily / Ribosomal protein L38e superfamily / Ribosomal L39 protein / Ribosomal protein L19, eukaryotic / Ribosomal protein L39e domain superfamily / Ribosomal protein L15e core domain superfamily / Ribosomal protein L5 eukaryotic/L18 archaeal, C-terminal / 60S ribosomal protein L4, C-terminal domain / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L28e/Mak16 / 50S ribosomal protein L30e-like / Ubiquitin-like domain superfamily / Ribosomal protein L15 / Ribosomal protein L5, C-terminal / Ribosomal protein L5, N-terminal / Ribosomal protein L7, eukaryotic/archaeal / Ribosomal protein L36e domain superfamily / Ribosomal protein L19/L19e superfamily / Ribosomal protein L29/L35 superfamily / Ribosomal L18e/L15P superfamily / Ribosomal protein L32e superfamily / Ribosomal protein L22/L17 superfamily / Ribosomal protein L6, alpha-beta domain superfamily / Ribosomal protein L14 superfamily / Ribosomal protein L13 superfamily / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L21 superfamily / Ribosomal L29 protein / Ribosomal protein L44 / UCH proteinases / Ribosomal protein L13e signature. / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L23 signature. / Ribosomal protein L39e signature. / Ubiquitin domain signature. / Ribosomal protein L2 signature. / Ribosomal protein L3 signature. / Ribosomal protein L15 signature. / Ribosomal protein L32e signature. / Ribosomal protein L30e signature 1. / Ribosomal protein L30e signature 2. / Ribosomal protein L37e signature. / Ribosomal protein L18e signature. / Ribosomal proteins L26 eukaryotic, L24P archaeal / Ribosomal protein L31e signature. / Ribosomal protein L34e signature. / Ribosomal protein L21e signature. / Ribosomal protein L44e signature. / Ribosomal protein L36e signature. / Ribosomal protein L15e signature. / Ribosomal protein L10e signature. / Ubiquitin domain profile. / Translesion Synthesis by POLH / L13a-mediated translational silencing of Ceruloplasmin expression / rt:r-pfa-1799339: / Ribosomal protein 60S L18 and 50S L18e / 60S ribosomal protein L4 C-terminal domain / Ribosomal L40e family / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L21e / Ribosomal protein L36e / Ribosomal protein L6e / Ribosomal protein L31e / Ribosomal protein L34e / Ribosomal protein L24e / Ribosomal protein L35Ae / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / Ribosomal protein L19e / Ribosomal protein L13e / Ribosomal protein L32 / Ribosomal L22e protein family / Ribosomal L18 C-terminal region / Ribosomal L27e protein family
Function and homology information
Specimen sourcePlasmodium falciparum 3D7 (eukaryote)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / 3.2 Å resolution
AuthorsWong, W. / Bai, X.-C. / Brown, A. / Scheres, S. / Baum, J.
CitationJournal: Nat Microbiol / Year: 2017
Title: Mefloquine targets the Plasmodium falciparum 80S ribosome to inhibit protein synthesis.
Authors: Wilson Wong / Xiao-Chen Bai / Brad E Sleebs / Tony Triglia / Alan Brown / Jennifer K Thompson / Katherine E Jackson / Eric Hanssen / Danushka S Marapana / Israel S Fernandez / Stuart A Ralph / Alan F Cowman / Sjors H W Scheres / Jake Baum
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Jan 27, 2017 / Release: Mar 1, 2017
RevisionDateData content typeGroupCategoryItemProviderType
1.0Mar 1, 2017Structure modelrepositoryInitial release
1.1Mar 22, 2017Structure modelDatabase references
1.2Aug 30, 2017Structure modelAuthor supporting evidence / Data collectionem_software / pdbx_audit_support_em_software.name / _pdbx_audit_support.funding_organization
1.3Oct 17, 2018Structure modelData collection / Refinement descriptionrefine

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Structure visualization

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Structure viewerMolecule:
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Assembly

Deposited unit
A: 28S ribosomal RNA
B: 5S ribosomal RNA
C: 5.8S ribosomal RNA
D: 60S ribosomal protein L2
E: 60S ribosomal protein L3
F: 60S ribosomal protein L4
G: 60S ribosomal protein L11a, putative
H: 60S ribosomal protein L6, putative
I: 60S ribosomal protein L6-2, putative
J: 60S ribosomal protein L7-3, putative
K: 60S ribosomal protein L13, putative
L: 60S ribosomal protein L13
M: 60S ribosomal protein L23, putative
N: 60S ribosomal protein L14, putative
O: 60S ribosomal protein L27a, putative
P: Ribosomal protein L15
Q: 60S ribosomal protein L10, putative
R: 60S ribosomal protein L5, putative
S: 60S ribosomal protein L18-2, putative
T: 60S ribosomal protein L19
U: 60S ribosomal protein L18a
V: 60S ribosomal protein L21
W: 60S ribosomal protein L17, putative
X: 60S ribosomal protein L22, putative
Y: 60S ribosomal protein L23
Z: 60S ribosomal protein L26, putative
0: 60S ribosomal protein L24, putative
1: 60S ribosomal protein L27
2: 60S ribosomal protein L28
3: 60S ribosomal protein L35, putative
4: 60S ribosomal protein L29, putative
5: 60S ribosomal protein L7, putative
6: 60S ribosomal protein L30e, putative
7: 60S ribosomal protein L31
8: 60S ribosomal protein L32
9: 60S ribosomal protein L35Ae, putative
a: 60S ribosomal protein L34
b: 60S ribosomal protein L36
c: Ribosomal protein L37
d: 60S ribosomal protein L38
e: 60S ribosomal protein L39
f: Ubiquitin-60S ribosomal protein L40
g: 60S ribosomal protein L41
h: 60S ribosomal protein L37a
i: 60S ribosomal protein L44
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,136,616216
Polyers2,131,54645
Non-polymers5,070171
Water0
1


  • idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area (Å2)406610
ΔGint (kcal/M)-4819
Surface area (Å2)581500
MethodPISA

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Components

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RNA chain , 3 types, 3 molecules ABC

#1: RNA chain 28S ribosomal RNA /


Mass: 1216212.125 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: GenBank: 1013064538
#2: RNA chain 5S ribosomal RNA /


Mass: 38410.859 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: GenBank: 1016052399
#3: RNA chain 5.8S ribosomal RNA /


Mass: 51206.461 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: GenBank: 1013064403

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60S ribosomal protein ... , 39 types, 39 molecules DEFGHIJKLMNOQRSTUVWXYZ01234567...

#4: Protein/peptide 60S ribosomal protein L2 /


Mass: 28064.998 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8I3T9
#5: Protein/peptide 60S ribosomal protein L3 / / 60S ribosomal protein L3 / putative


Mass: 44318.180 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IJC6
#6: Protein/peptide 60S ribosomal protein L4 /


Mass: 46311.688 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8I431
#7: Protein/peptide 60S ribosomal protein L11a, putative / Ribosome


Mass: 20268.666 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IBQ6
#8: Protein/peptide 60S ribosomal protein L6, putative / Ribosome


Mass: 21638.562 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IE85
#9: Protein/peptide 60S ribosomal protein L6-2, putative / Ribosome


Mass: 25587.623 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IDV1
#10: Protein/peptide 60S ribosomal protein L7-3, putative / Ribosome


Mass: 32760.455 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8ILL2
#11: Protein/peptide 60S ribosomal protein L13, putative / Ribosome


Mass: 23805.344 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IJZ7
#12: Protein/peptide 60S ribosomal protein L13 /


Mass: 25454.143 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IAX6
#13: Protein/peptide 60S ribosomal protein L23, putative / Ribosome


Mass: 15015.713 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IE09
#14: Protein/peptide 60S ribosomal protein L14, putative / Ribosome


Mass: 19340.373 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8ILE8
#15: Protein/peptide 60S ribosomal protein L27a, putative / Ribosome


Mass: 16765.834 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: C6KT23
#17: Protein/peptide 60S ribosomal protein L10, putative / Ribosome


Mass: 25265.480 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8ILV2
#18: Protein/peptide 60S ribosomal protein L5, putative / Ribosome


Mass: 34067.352 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8ILL3
#19: Protein/peptide 60S ribosomal protein L18-2, putative / Ribosome


Mass: 21508.783 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: C0H5G3
#20: Protein/peptide 60S ribosomal protein L19 /


Mass: 21641.096 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: C6KSY6
#21: Protein/peptide 60S ribosomal protein L18a /


Mass: 21793.611 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IDS6
#22: Protein/peptide 60S ribosomal protein L21 / / 60S ribosomal protein L21e / putative


Mass: 18840.244 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8ILK3
#23: Protein/peptide 60S ribosomal protein L17, putative / Ribosome


Mass: 23469.926 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IDI5
#24: Protein/peptide 60S ribosomal protein L22, putative / Ribosome


Mass: 16422.930 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IB51
#25: Protein/peptide 60S ribosomal protein L23 /


Mass: 22152.748 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IE82
#26: Protein/peptide 60S ribosomal protein L26, putative / Ribosome


Mass: 14855.709 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: O77364
#27: Protein/peptide 60S ribosomal protein L24, putative / Ribosome


Mass: 19295.930 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IEM3
#28: Protein/peptide 60S ribosomal protein L27 / / 60S ribosomal protein L27 / putative


Mass: 16785.180 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IKM5
#29: Protein/peptide 60S ribosomal protein L28 / / 60S ribosomal protein L28 / putative


Mass: 14437.823 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IHU0
#30: Protein/peptide 60S ribosomal protein L35, putative / Ribosome


Mass: 14787.824 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IIB4
#31: Protein/peptide 60S ribosomal protein L29, putative / Ribosome / Ribosomal protein L29 / putative


Mass: 8041.588 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: C6S3J6
#32: Protein/peptide 60S ribosomal protein L7, putative / Ribosome


Mass: 30593.184 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: O97250
#33: Protein/peptide 60S ribosomal protein L30e, putative / Ribosome


Mass: 11794.760 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IJK8
#34: Protein/peptide 60S ribosomal protein L31 /


Mass: 14099.854 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8I463
#35: Protein/peptide 60S ribosomal protein L32 /


Mass: 15565.751 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8I3B0
#36: Protein/peptide 60S ribosomal protein L35Ae, putative / Ribosome / 60S ribosomal protein L35ae / putative


Mass: 16306.425 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IHT9
#37: Protein/peptide 60S ribosomal protein L34 /


Mass: 17406.967 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8IBY4
#38: Protein/peptide 60S ribosomal protein L36 /


Mass: 12825.440 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8I713
#40: Protein/peptide 60S ribosomal protein L38 / / 60S ribosomal protein L38e / putative


Mass: 10341.729 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8II62
#41: Protein/peptide 60S ribosomal protein L39 /


Mass: 6540.912 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: C0H4H3
#43: Protein/peptide 60S ribosomal protein L41 / / 60S ribosomal protein L41 / putative


Mass: 5108.310 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: C6S3G4
#44: Protein/peptide 60S ribosomal protein L37a /


Mass: 10826.083 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: O96184
#45: Protein/peptide 60S ribosomal protein L44 /


Mass: 12194.902 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: O97231

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Ribosomal protein ... , 2 types, 2 molecules Pc

#16: Protein/peptide Ribosomal protein L15 /


Mass: 24197.660 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: C0H4A6
#39: Protein/peptide Ribosomal protein L37 /


Mass: 10571.592 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: C0H4L5

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Protein/peptide , 1 types, 1 molecules f

#42: Protein/peptide Ubiquitin-60S ribosomal protein L40


Mass: 14645.169 Da / Num. of mol.: 1 / Source: (natural) Plasmodium falciparum 3D7 (eukaryote) / References: UniProt: Q8ID50

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Non-polymers , 3 types, 171 molecules

#46: Chemical ChemComp-YMZ / Mefloquine


Mass: 378.312 Da / Num. of mol.: 2 / Formula: C17H16F6N2O / Mefloquine
#47: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 164 / Formula: Mg / Magnesium
#48: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 5 / Formula: Zn / Zinc

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / Reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Plasmodium falciparum 80S ribosome bound to mefloquine
Type: RIBOSOME
Entity ID: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45
Source: NATURAL
Molecular weightValue: 4.2 MDa / Experimental value: NO
Source (natural)Cellular location: cytoplasm / Organism: Plasmodium falciparum 3D7 (eukaryote)
Buffer solutionDetails: 20 mM Hepes pH 7.4, 40 mM KAc, 10 mM NH4Ac, 10 mM Mg(Ac)2 and 5 mM 2-mecaptoethanol
pH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: Blot 2.5s before plunging / Grid material: COPPER / Grid type: Quantifoil R2/2
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 90 kelvins

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Electron microscopy imaging

Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI POLARA 300
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 78000 / Calibrated magnification: 104748 / Calibrated defocus min: 800 nm / Calibrated defocus max: 3800 nm / Cs: 2 mm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: GATAN LIQUID NITROGEN / Temperature (max): 90 kelvins / Temperature (min): 80 kelvins
Image recordingElectron dose: 20 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k) / Number of grids imaged: 1
Image scansMovie frames/image: 17

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Processing

SoftwareName: REFMAC / Version: 5.8.0068 / Classification: refinement
EM software
IDNameVersionCategory
2RELION1.3image acquisition
4CTFFIND3CTF correction
7UCSF Chimeramodel fitting
9RELION1.3initial Euler assignment
10RELION1.3final Euler assignment
11RELION1.3classification
12RELION1.33D reconstruction
13REFMAC5.8model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C1
3D reconstructionResolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 43184 / Symmetry type: POINT
Atomic model buildingOverall b value: 129.3 / Ref protocol: RIGID BODY FIT / Ref space: RECIPROCAL
RefineCorrelation coeff Fo to Fc: 0.952 / Overall SU B: 29.102 / Overall SU ML: 0.428 / Overall ESU R: 0.567
Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES
Solvent computationSolvent ion probe radii: 0.8 Å / Solvent shrinkage radii: 0.8 Å / Solvent vdw probe radii: 1.2 Å / Solvent model details: MASK
Displacement parametersB iso mean: 129.307 Å2 / Aniso B11: -0.25 Å2 / Aniso B12: 0.09 Å2 / Aniso B13: 0.15 Å2 / Aniso B22: 0.17 Å2 / Aniso B23: -0.02 Å2 / Aniso B33: 0.08 Å2
Least-squares processR factor R work: 0.27921 / R factor obs: 0.27921 / Highest resolution: 3.2 Å / Lowest resolution: 257.5 Å / Number reflection obs: 806977 / Percent reflection obs: 1
Number of atoms included #1Total: 124503
Refine LS restraints
Refine IDTypeDev idealDev ideal targetNumber
ELECTRON MICROSCOPYr_bond_refined_d0.0050.014136485
ELECTRON MICROSCOPYr_bond_other_d
ELECTRON MICROSCOPYr_angle_refined_deg0.8751.526196987
ELECTRON MICROSCOPYr_angle_other_deg
ELECTRON MICROSCOPYr_dihedral_angle_1_deg24.1856.9827685
ELECTRON MICROSCOPYr_dihedral_angle_2_deg33.18621.8972119
ELECTRON MICROSCOPYr_dihedral_angle_3_deg17.84215.00010690
ELECTRON MICROSCOPYr_dihedral_angle_4_deg11.95715.000538
ELECTRON MICROSCOPYr_chiral_restr0.3390.21322289
ELECTRON MICROSCOPYr_gen_planes_refined0.0050.02072478
ELECTRON MICROSCOPYr_gen_planes_other
ELECTRON MICROSCOPYr_nbd_refined
ELECTRON MICROSCOPYr_nbd_other
ELECTRON MICROSCOPYr_nbtor_refined
ELECTRON MICROSCOPYr_nbtor_other
ELECTRON MICROSCOPYr_xyhbond_nbd_refined
ELECTRON MICROSCOPYr_xyhbond_nbd_other
ELECTRON MICROSCOPYr_metal_ion_refined
ELECTRON MICROSCOPYr_metal_ion_other
ELECTRON MICROSCOPYr_symmetry_vdw_refined
ELECTRON MICROSCOPYr_symmetry_vdw_other
ELECTRON MICROSCOPYr_symmetry_hbond_refined
ELECTRON MICROSCOPYr_symmetry_hbond_other
ELECTRON MICROSCOPYr_symmetry_metal_ion_refined
ELECTRON MICROSCOPYr_symmetry_metal_ion_other
ELECTRON MICROSCOPYr_mcbond_it1.34413.26824887
ELECTRON MICROSCOPYr_mcbond_other
ELECTRON MICROSCOPYr_mcangle_it2.49619.89131002
ELECTRON MICROSCOPYr_mcangle_other
ELECTRON MICROSCOPYr_scbond_it1.19012.811111598
ELECTRON MICROSCOPYr_scbond_other
ELECTRON MICROSCOPYr_scangle_it
ELECTRON MICROSCOPYr_scangle_other
ELECTRON MICROSCOPYr_long_range_B_refined7.980518007
ELECTRON MICROSCOPYr_long_range_B_other
ELECTRON MICROSCOPYr_rigid_bond_restr
ELECTRON MICROSCOPYr_sphericity_free
ELECTRON MICROSCOPYr_sphericity_bonded
Refine LS shellHighest resolution: 3.2 Å / R factor R work: 0.576 / Lowest resolution: 3.283 Å / Number reflection R free: 0 / Number reflection R work: 59775 / Total number of bins used: 20 / Percent reflection obs: 1

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