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- EMDB-8576: Structure of the Plasmodium falciparum 80S ribosome bound to the ... -

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Basic information

Entry
Database: EMDB / ID: 8576
TitleStructure of the Plasmodium falciparum 80S ribosome bound to the antimalarial drug mefloquine
SamplePlasmodium falciparum 80S ribosome bound to mefloquine
SourcePlasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum
Map data
Methodsingle particle reconstruction, at 3.2 A resolution
AuthorsWong W / Bai X-C
CitationNat Microbiol, 2017, 2, 17031-17031

Nat Microbiol, 2017, 2, 17031-17031 StrPapers
Mefloquine targets the Plasmodium falciparum 80S ribosome to inhibit protein synthesis.
Wilson Wong / Xiao-Chen Bai / Brad E Sleebs / Tony Triglia / Alan Brown / Jennifer K Thompson / Katherine E Jackson / Eric Hanssen / Danushka S Marapana / Israel S Fernandez / Stuart A Ralph / Alan F Cowman / Sjors H W Scheres / Jake Baum

DateDeposition: Jan 27, 2017 / Header (metadata) release: Mar 1, 2017 / Map release: Mar 1, 2017 / Last update: Jan 27, 2017

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.12
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by height
  • Surface level: 0.12
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view with fitted model
  • Atomic models: : PDB-5umd
  • Surface level: 0.12
  • Imaged by UCSF CHIMERA
  • Download
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Supplemental images

Downloads & links

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Map

Fileemd_8576.map.gz (map file in CCP4 format, 364501 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
450 pix
1.03 A/pix
= 463.5 A
450 pix
1.03 A/pix
= 463.5 A
450 pix
1.03 A/pix
= 463.5 A

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 1.03 A
Density
Contour Level:0.12 (by author), 0.12 (movie #1):
Minimum - Maximum-0.5065006 - 0.8825569
Average (Standard dev.)0.0001856431 (0.033729594)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions450450450
Origin000
Limit449449449
Spacing450450450
CellA=B=C: 603 A
Alpha=beta=gamma: 90 deg.

CCP4 map header:

modeImage stored as Reals
A/pix X/Y/Z1.031.031.03
M x/y/z450450450
origin x/y/z0.0000.0000.000
length x/y/z463.500463.500463.500
alpha/beta/gamma90.00090.00090.000
start NX/NY/NZ
NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS450450450
D min/max/mean-0.5070.8830.000

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Supplemental data

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Sample components

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Entire Plasmodium falciparum 80S ribosome bound to mefloquine

EntireName: Plasmodium falciparum 80S ribosome bound to mefloquine
Number of components: 49
MassTheoretical: 4.2 MDa

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Component #1: protein, Plasmodium falciparum 80S ribosome bound to mefloquine

ProteinName: Plasmodium falciparum 80S ribosome bound to mefloquine
Recombinant expression: No
MassTheoretical: 4.2 MDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum
Source (natural)Location in cell: cytoplasm

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Component #2: nucleic-acid, 28S ribosomal RNA

Nucleic-acidName: 28S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GUUUGAUUUG AUGUUAGGAG GGCAAAUCCG CCGAAUUUAA GCAUAUAAUU AAGCGGAAGA AAAGAAAAUA ACUAUGAUUC CUUUAGUAAC GGCGAGUGAA CAAGGACCAG CUCAAACGGU UAAUCCUUCG UUUGGUGCAA ACCUUUCGAC GGAAUUGUCG UUGAAUUAUU CCAUGAAUGC CUUUAAUUUG AGCAAAGUGA GCAGGAAUGC UUCCCCAUAG AGGGUGAAAG GCCCAUAGUU CUUUUUAAUU UAUUCAUUGG AAUUUUUCAU GUCGAGUCGU GUUCUUUGAG AUUGGAGCAC UAAUACGUGU GAUACAUUUC ACAUAAAGCU AAAUAUAUGU AGGAGACCGA UAGCAAACAA GUACCGUGAG GGAAAGAUGA AAUAGUACUC AGGAAUGAGC AAUUAAAUAG UACCUGAAAU CGUUAAGAUG GAACGGAUUA AGAGAGAAAA CAAGUAAAGA GGGGAAUUUU UAAUUUUUUU UGUUAUAAUU CUCUUCUUUA UUAAAAGAAA CAUCAGUGAU UAAUUUAAUU UCAAUAAAGC AAUCCCCUGA AAUUCAAAAU UUCUUUUAAU UUUGUUUUCA CUUUCUCCCC GCACUAAUGU GGGGAAAACU GGCUUUAUUU CUUCAAUUAU UUUUUUUGCU GAGGAAUUUU AAAAUUAUUU GAAUUUUUCU UCUUAUAAUU UAAGUUGUUU CUAUACAGUA CUUUCUUAAC CCACUCGUCU UGAAACACGG ACCAAGGAGU CUAGCAAAUG UGCAAGUGUA UAUGAUUCUU UAAACAUUUC UCUUUUUAAU AUACGCAUAA UUAAUGUAAU AUGUUUUCUU CAUUGUAGAU UUGUGGUGUU UAAUUUUUAU UAAAUCCCCA CUUUGCAUAC AAUACCGGUA AGCAAUUAUG CUUUAUUGAG UACGAGCAUA UUUGGUAGGA CCCGAGAGGC UUUGAACUAA GCGUGAUGAG AUUGAAGUCA GACGAAAGUC UGAUGGAGGA UCGAGUUGAU ACUGACGUGC AAAUCGUUCA UUUCAAUCAC GUUUAGGGGC GAAAGACUAA UCGAAAAGCC UAUUAGCUGG UUAUUUUCGA AAGAUCUCUC AGGAUCGCUG GAGUUGAUUA AUAUAAUUUU ACCAGGUAGA GAUAAUGAUU AGAGGAAUCA GGGAAAUCGU UUUCCUUGAC CUAUUCUCAA ACUCCCAAUA GGUAAAAAGG GCGAAAUAAC UCAAUUUGUA UUUCACUCAU AUUAAAUAAA AUAACUCCAA GUGGGCCAUU UUUGGUAAGC AGAACUGGCG AUGAGGGAUG CUCCUAACGC UUGGAUAAGG UGCCUAAAUA UUCGCUCAUG AGAUCCCAUA AAAGGUGUUG GUUCAUAAUG ACAGUAGGAC GGUGGUCAUG GAAGUCGAAA UCCGCUAAGG AGUGUGUAAC AACUCACCUA CCGAAUGAAC UAGCCCUGAA AAUGGAUGGC GCUAAAGCGG AUUACCGAUA CCAAGCCAUA AAAAGAACAG AAAAAAUUUA UUUUUUUUUU AGAAUCUUUU UAUGAGUAGA AAAUCGUGGG GUUUGUGUUG AAGCGAAAUA CGUGAGUUUU CGUGGAACAU CUCCCUAGUG CAGAUCUUGG UGGAAGUAGC AACUAUUCAA AUGAGAACUU UGAAGACUGA AGUGGAGAAG GGUUUCUUGU CAACUGUGAU UGAACAAGAG UUAGCCGCUC CUAAGGGAUA GCUGAAAAGU GUUUAAAAGG GGGUUCCUUC CCCGUCUCAA AAGGGAAACA GGUUGAUAUU CCUGUGCCAA UAGUAUUAUG AGUUUCUUAG AUGGUAACAU AUAUAUAAAU GAACUCCUUU ACAUAGGCUU UACACUCGGG GUGCGUUUUC UUUGCACUUU ACCUUUAUAA CAAACCUUGG AAUCAAUUUA CUUGGAGAAG AGGUUCGUUG AACUCAAUUC AAAAAAUUUC UUGAACGGGG UUUUCGGAUU CAGUUCAUUU UAUUUUUUGU UUUGUAGCAA UAGUAAUUCG UUUUUAUGAA UUAUCCGAAG UGGUAAGGAC UAUCCUUGAA AAAAGGAGGG AACGGCUUUG UAACUUGGUU UCUUCUGAUU CCAUUUUGCU UUGCUUAUAC UCUUAAUACU UGUAUGAGCG UACCAACAAC CGCAUCAGGU CUCCAAGGUU AACAGCCUCU GGUUAAAUAG AAAAAAGUAG GUAAGGGAAG UCGGCAAAAU AGAUCCGUAA CUUCGGGAAA AGGAUUGGCU CUGAGGACAU UAGAAAAGAG AAGAAAAAAA GAGGGUCGAA AAUAAAAUUG CAGUCUUUAU UUGCUUUUCU UUCGAUUUGC UUGUAAUCUG CUUUUUCUUU UUCUUCUUUC UUUUUUUCUC CCCCUCUUUU CGCCUUCACU UUAUUGUAAU UUUAUUACUU UAAUUUGAUA CCUAUAAUGU UAACUCAGAA CUGAAACGGA CAAGGGGAAU CCGACUGUUU AAUUAAAACA UAGCAUUGUG AAAAGCCACA ACUGGUAUUA ACACAAUGUG AUUUCUGCCC AGUGCUUUGA AUGUCAACUU GAUGAAAUUC AAUCAAGCGC AGGUAAACGG CGGGAGUAAC UAUGACUCUC UUAAGGUAGC CAAAUGCCUC GUCAUCUAAU UAGUGACGCG CAUGAAUGGA UUAACGAGAU UCCCACUGUC CCUACUUGCU AUCUAGCGAA ACCACAGCCA AGGGAACGGG CUUGGCAAAA UCAGCGGGGA AAGAAGACCC UGUUGAGCUU UACUCUAGUC UGGCUUUGUG AAACGACUUA AGAGGUGUAG CAUAAGUGGG AGUAGAAACU GAAAUAUGUU UUUACGACAG UGAAAUACCA CUACUUUUAA AGUUGUUUUA CUUAUCCAUU UAUAGGGAAA UAUAUUAUGC UUUAUCCUUC GGGAAGGCAU UCUGAUAUUA UUUGAAUUUU UAAGAAACUU GUUUCUUAUC UUUCUCCCAA UUUCUAUGGA GACAUAGCCA GGUGGGGAGU UUGACUGGGG CGGUACAUCU GUUAAAUAUU AACGCAGAUG UCCAAAGACA AGCUCAAAGA GAACAGAAAU CUCUUGUAGA CUAAAAGGGG AAAAGCUUGU UUGAUUUCUA UUUUCAGAAC AAGUAGAAAA CGUGAAAGCG UGGCCUAUCG AUCCUUUAUA UUUGCAAAAU GACGUAAAUU ACUUACUACU GUGCAUAUAG AGGUGUCUGA AAAGUUACCA CAGGGAUAAC UGGCUUGUGG CUGCCAAGCG CUCCUAGCGA CGUAGCUUUU UGAUCCUUCG AUGUCGGCUC UUCCUAUCAU UGGGACGCAG AAGUCUCAAA GUGUCGGAUU GUUCACCCGC UAAUAGGGAA CGUGAGCUGG GUUUAGACCG UCGUGAGACA GGUUAGUUUU ACCCUACUGA UGAAUUUUAU UAUAUUUUUU AUAUAUACAU AUAGUAUUGU GACAGUAAUC CAACUUGGUA CGAGAGGAUU AGUUGGUUCA GACAAUUGGU ACAGCAAUUG GUUGACAAAC CAGUGUUGCG AAGCUAAGUC UGUUGGAUAA UGGCUGAACG CCUCUUAAGC CAGAAACCAU GCUGAUUAAA CAAUACUAUU UCGUUCUUUU UUGUUUCCUU UUUUACUGCU AAUGUAAAAG AGAAAUUAUA UAUGUACCUU UUUUUGGUAC AUAAAUAAUC CUAUCACCUU UUUCGUGAGC UGUGAGCUGU GAGCUAGGCA AUAGUUCUUU AACAACAAAA AAGAAUUGCA CAAACUGUAG ACGACUUUUU UGCCUCAGGG UGCUGUAAAC AUGAAAGUAA ACUUUGUUUU ACGAUCUGUU GAGGCUUAUC CCUAGUGGUA AAGUGUUU
MassTheoretical: 1.216212125 MDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #3: nucleic-acid, 5S ribosomal RNA

Nucleic-acidName: 5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UGACUCGUUC AUACUACAGU GGAUACACCA GAUCCCAUCA GAACUCUGAA GUUAAGCACU GUAAGGCUUG GCUAGUACUG AGGUGGGAGA CCGCUCGGGA ACACCAGGUG AUGAGUCAG
MassTheoretical: 38.410859 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #4: nucleic-acid, 5.8S ribosomal RNA

Nucleic-acidName: 5.8S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UAGCACAGUC UUAACGAUGG AUGUCUUGGU UCCUACAGCG AUGAAGGCCG CAGCAAAAUG CGAUACGCAA UGAAAAUUGC AGUGACUGUG AAUCAUCAGA AUGCUGAAUG UAAACUACAC CAAUUCCCUU CGGGGAAUAG UGGUACUCCU ACAGAAAAU
MassTheoretical: 51.206461 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #5: protein, 60S ribosomal protein L2

ProteinName: 60S ribosomal protein L2 / Recombinant expression: No
MassTheoretical: 28.064998 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #6: protein, 60S ribosomal protein L3

ProteinName: 60S ribosomal protein L3 / Recombinant expression: No
MassTheoretical: 44.31818 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #7: protein, 60S ribosomal protein L4

ProteinName: 60S ribosomal protein L4 / Recombinant expression: No
MassTheoretical: 46.311688 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #8: protein, 60S ribosomal protein L11a, putative

ProteinName: 60S ribosomal protein L11a, putative / Recombinant expression: No
MassTheoretical: 20.268666 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #9: protein, 60S ribosomal protein L6, putative

ProteinName: 60S ribosomal protein L6, putative / Recombinant expression: No
MassTheoretical: 21.638562 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #10: protein, 60S ribosomal protein L6-2, putative

ProteinName: 60S ribosomal protein L6-2, putative / Recombinant expression: No
MassTheoretical: 25.587623 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #11: protein, 60S ribosomal protein L7-3, putative

ProteinName: 60S ribosomal protein L7-3, putative / Recombinant expression: No
MassTheoretical: 32.760455 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #12: protein, 60S ribosomal protein L13, putative

ProteinName: 60S ribosomal protein L13, putative / Recombinant expression: No
MassTheoretical: 23.805344 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #13: protein, 60S ribosomal protein L13

ProteinName: 60S ribosomal protein L13 / Recombinant expression: No
MassTheoretical: 25.454143 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #14: protein, 60S ribosomal protein L23, putative

ProteinName: 60S ribosomal protein L23, putative / Recombinant expression: No
MassTheoretical: 15.015713 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #15: protein, 60S ribosomal protein L14, putative

ProteinName: 60S ribosomal protein L14, putative / Recombinant expression: No
MassTheoretical: 19.340373 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #16: protein, 60S ribosomal protein L27a, putative

ProteinName: 60S ribosomal protein L27a, putative / Recombinant expression: No
MassTheoretical: 16.765834 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #17: protein, Ribosomal protein L15

ProteinName: Ribosomal protein L15 / Recombinant expression: No
MassTheoretical: 24.19766 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #18: protein, 60S ribosomal protein L10, putative

ProteinName: 60S ribosomal protein L10, putative / Recombinant expression: No
MassTheoretical: 25.26548 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #19: protein, 60S ribosomal protein L5, putative

ProteinName: 60S ribosomal protein L5, putative / Recombinant expression: No
MassTheoretical: 34.067352 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #20: protein, 60S ribosomal protein L18-2, putative

ProteinName: 60S ribosomal protein L18-2, putative / Recombinant expression: No
MassTheoretical: 21.508783 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #21: protein, 60S ribosomal protein L19

ProteinName: 60S ribosomal protein L19 / Recombinant expression: No
MassTheoretical: 21.641096 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #22: protein, 60S ribosomal protein L18a

ProteinName: 60S ribosomal protein L18a / Recombinant expression: No
MassTheoretical: 21.793611 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #23: protein, 60S ribosomal protein L21

ProteinName: 60S ribosomal protein L21 / Recombinant expression: No
MassTheoretical: 18.840244 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #24: protein, 60S ribosomal protein L17, putative

ProteinName: 60S ribosomal protein L17, putative / Recombinant expression: No
MassTheoretical: 23.469926 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #25: protein, 60S ribosomal protein L22, putative

ProteinName: 60S ribosomal protein L22, putative / Recombinant expression: No
MassTheoretical: 16.42293 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #26: protein, 60S ribosomal protein L23

ProteinName: 60S ribosomal protein L23 / Recombinant expression: No
MassTheoretical: 22.152748 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #27: protein, 60S ribosomal protein L26, putative

ProteinName: 60S ribosomal protein L26, putative / Recombinant expression: No
MassTheoretical: 14.855709 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #28: protein, 60S ribosomal protein L24, putative

ProteinName: 60S ribosomal protein L24, putative / Recombinant expression: No
MassTheoretical: 19.29593 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #29: protein, 60S ribosomal protein L27

ProteinName: 60S ribosomal protein L27 / Recombinant expression: No
MassTheoretical: 16.78518 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #30: protein, 60S ribosomal protein L28

ProteinName: 60S ribosomal protein L28 / Recombinant expression: No
MassTheoretical: 14.437823 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #31: protein, 60S ribosomal protein L35, putative

ProteinName: 60S ribosomal protein L35, putative / Recombinant expression: No
MassTheoretical: 14.787824 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #32: protein, 60S ribosomal protein L29, putative

ProteinName: 60S ribosomal protein L29, putative / Recombinant expression: No
MassTheoretical: 8.041588 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #33: protein, 60S ribosomal protein L7, putative

ProteinName: 60S ribosomal protein L7, putative / Recombinant expression: No
MassTheoretical: 30.593184 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #34: protein, 60S ribosomal protein L30e, putative

ProteinName: 60S ribosomal protein L30e, putative / Recombinant expression: No
MassTheoretical: 11.79476 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #35: protein, 60S ribosomal protein L31

ProteinName: 60S ribosomal protein L31 / Recombinant expression: No
MassTheoretical: 14.099854 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #36: protein, 60S ribosomal protein L32

ProteinName: 60S ribosomal protein L32 / Recombinant expression: No
MassTheoretical: 15.565751 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #37: protein, 60S ribosomal protein L35Ae, putative

ProteinName: 60S ribosomal protein L35Ae, putative / Recombinant expression: No
MassTheoretical: 16.306425 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #38: protein, 60S ribosomal protein L34

ProteinName: 60S ribosomal protein L34 / Recombinant expression: No
MassTheoretical: 17.406967 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #39: protein, 60S ribosomal protein L36

ProteinName: 60S ribosomal protein L36 / Recombinant expression: No
MassTheoretical: 12.82544 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #40: protein, Ribosomal protein L37

ProteinName: Ribosomal protein L37 / Recombinant expression: No
MassTheoretical: 10.571592 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #41: protein, 60S ribosomal protein L38

ProteinName: 60S ribosomal protein L38 / Recombinant expression: No
MassTheoretical: 10.341729 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #42: protein, 60S ribosomal protein L39

ProteinName: 60S ribosomal protein L39 / Recombinant expression: No
MassTheoretical: 6.540912 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #43: protein, Ubiquitin-60S ribosomal protein L40

ProteinName: Ubiquitin-60S ribosomal protein L40 / Recombinant expression: No
MassTheoretical: 14.645169 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #44: protein, 60S ribosomal protein L41

ProteinName: 60S ribosomal protein L41 / Recombinant expression: No
MassTheoretical: 5.10831 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #45: protein, 60S ribosomal protein L37a

ProteinName: 60S ribosomal protein L37a / Recombinant expression: No
MassTheoretical: 10.826083 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #46: protein, 60S ribosomal protein L44

ProteinName: 60S ribosomal protein L44 / Recombinant expression: No
MassTheoretical: 12.194902 kDa
SourceSpecies: Plasmodium falciparum 3d7 / eukaryote / image: Plasmodium falciparum

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Component #47: ligand, Mefloquine

LigandName: Mefloquine / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 0.378312 kDa

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Component #48: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 164 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

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Component #49: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 5 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

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Experimental details

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Sample preparation

Specimen stateparticle
Sample solutionBuffer solution: 20 mM Hepes pH 7.4, 40 mM KAc, 10 mM NH4Ac, 10 mM Mg(Ac)2 and 5 mM 2-mecaptoethanol
pH: 7.4
Support filmBlot 2.5s before plunging
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 90 K / Humidity: 100 %

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Electron microscopy imaging

ImagingMicroscope: FEI POLARA 300
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 20 e/A2 / Illumination mode: FLOOD BEAM
LensMagnification: 78000 X (nominal), 104748 X (calibrated) / Cs: 2 mm / Imaging mode: BRIGHT FIELD
Specimen HolderModel: GATAN LIQUID NITROGEN / Temperature: K ( 80 - 90 K)
CameraDetector: FEI FALCON II (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 43184
3D reconstructionSoftware: Relion / Resolution: 3.2 A / Resolution method: FSC 0.143 CUT-OFF

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Atomic model buiding

Output model

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