Ribosomal protein L14 superfamily / Ribosomal protein L40e superfamily / Shwachman-Bodian-Diamond syndrome (SBDS) protein / Ribosomal L40e family / Elongation factor G C-terminus / Ribosomal protein L10 / Ribosomal protein L6 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L3 / Ribosomal protein L16p/L10e ...Ribosomal protein L14 superfamily / Ribosomal protein L40e superfamily / Shwachman-Bodian-Diamond syndrome (SBDS) protein / Ribosomal L40e family / Elongation factor G C-terminus / Ribosomal protein L10 / Ribosomal protein L6 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L3 / Ribosomal protein L16p/L10e / Ubiquitin family / Ribosomal protein L14p/L23e / Elongation factor Tu GTP binding domain / Ribosome maturation protein Sdo1/SBDS-like / Ribosomal protein L24e/L24 superfamily / Ribosome maturation protein Sdo1/SBDS, central domain superfamily / eIF-6 family / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L6, alpha-beta domain superfamily / Ribosome maturation protein SBDS, N-terminal domain superfamily / Ribosomal protein L11, C-terminal domain superfamily / EF-G domain III/V-like / 60S acidic ribosomal protein P0 / Ubiquitin-like domain superfamily / P-loop containing nucleoside triphosphate hydrolase / Ribosomal protein L24e, conserved site / Ribosomal protein L11, N-terminal / Ribosomal protein L11, C-terminal / Ribosomal protein S5 domain 2-type fold / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L24e / Ribosomal protein L11, N-terminal domain / Ubiquitin conserved site / SRP-dependent cotranslational protein targeting to membrane / Formation of TC-NER Pre-Incision Complex / Formation of Incision Complex in GG-NER / DNA Damage Recognition in GG-NER / Metalloprotease DUBs / Ub-specific processing proteases / Josephin domain DUBs / UCH proteinases / Negative regulation of MAPK pathway / Termination of translesion DNA synthesis / Translesion synthesis by POLK / Hedgehog 'on' state / Hedgehog ligand biogenesis / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Autodegradation of the E3 ubiquitin ligase COP1 / APC-Cdc20 mediated degradation of Nek2A / SBDS protein C-terminal domain / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 / Autodegradation of Cdh1 by Cdh1:APC/C / APC/C:Cdc20 mediated degradation of Cyclin B / L13a-mediated translational silencing of Ceruloplasmin expression / Downregulation of ERBB2:ERBB3 signaling / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ubiquitin domain profile. / Uncharacterized protein family UPF0023 signature. / Ribosomal protein L10e signature. / Ribosomal protein L24e signature. / Ribosomal protein L6 signature 2. / Ribosomal protein L3 signature. / Ubiquitin domain signature. / Ubiquitin / Ribosomal protein L3, conserved site / Gap-filling DNA repair synthesis and ligation in TC-NER / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Cyclin D associated events in G1 / Ribosome maturation protein SBDS, N-terminal / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / Formation of a pool of free 40S subunits / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / GTP hydrolysis and joining of the 60S ribosomal subunit / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / E3 ubiquitin ligases ubiquitinate target proteins / Ribosomal protein L14P / Regulation of PTEN localization / Regulation of PTEN stability and activity / ER Quality Control Compartment (ERQC) / Peroxisomal protein import / Endosomal Sorting Complex Required For Transport (ESCRT) / Iron uptake and transport / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Antigen processing: Ubiquitination & Proteasome degradation / Orc1 removal from chromatin / Dual incision in TC-NER / Ribosomal protein L3 / Ribosomal protein L40e / Ribosomal protein L10P / Ribosomal protein L10e / Ubiquitin domain / Ribosomal protein L24e-related / Zinc-binding ribosomal protein / Ribosomal protein S5 domain 2-type fold, subgroup / Small GTP-binding protein domain / Translation initiation factor IF6
Function and homology information
Source
Dictyostelium discoideum (Slime Mold) / Homo sapiens (human)
Method
single particle reconstruction / cryo EM / 4.1 Å resolution
Buffer solution: 50 mM HEPES-KOH, 100 mM K(CH3COO), 10 mM Mg(CH3COO)2, 6 mM beta-mercaptoethanol pH: 7.5
Support film
quantifoil R2/2 glow discharged
Vitrification
Instrument: FEI VITROBOT MARK III / Cryogen name: ETHANE / Temperature: 120 K / Humidity: 100 % / Method: 6.5s blot
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Electron microscopy imaging #1
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
Imaging
Microscope: FEI TITAN KRIOS / Date: Sep 3, 2013
Electron gun
Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 30 e/Å2 / Illumination mode: FLOOD BEAM
Lens
Magnification: 59000 X (nominal), 105263 X (calibrated) Astigmatism: Objective lens astigmatism was corrected at 59,000 times magnification Cs: 2.2 mm / Imaging mode: BRIGHT FIELD / Defocus: 2200 - 2800 nm
Specimen Holder
Model: FEI TITAN KRIOS AUTOGRID HOLDER
Camera
Detector: FEI FALCON II (4k x 4k)
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Electron microscopy imaging #2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
Imaging
Microscope: FEI TITAN KRIOS / Date: Sep 6, 2013
Electron gun
Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 30 e/Å2 / Illumination mode: FLOOD BEAM
Lens
Magnification: 59000 X (nominal), 105263 X (calibrated) Astigmatism: Objective lens astigmatism was corrected at 59,000 times magnification Cs: 2.2 mm / Imaging mode: BRIGHT FIELD / Defocus: 2200 - 2800 nm
Specimen Holder
Model: FEI TITAN KRIOS AUTOGRID HOLDER
Camera
Detector: FEI FALCON II (4k x 4k)
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Image acquisition
Image acquisition
Number of digital images: 3844 Details: Every image is the average of 16 frames recorded by the direct electron detector
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Image processing
Processing
Method: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 11970
3D reconstruction
Software: RELION / CTF correction: Each particle / Resolution: 4.1 Å / Resolution method: FSC 0.143, gold-standard
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