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- EMDB-20077: Subunit joining exposes nascent pre-40S rRNA for processing and q... -

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Basic information

Entry
Database: EMDB / ID: EMD-20077
TitleSubunit joining exposes nascent pre-40S rRNA for processing and quality control
Map data
Sample
  • Complex: 60S subunit of 80S-like ribosome
    • Protein or peptide: x 45 types
    • RNA: x 3 types
Keywords80S-like complex / 60S / RIBOSOME
Function / homology
Function and homology information


pre-mRNA 5'-splice site binding / response to cycloheximide / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / Formation of a pool of free 40S subunits / preribosome, large subunit precursor ...pre-mRNA 5'-splice site binding / response to cycloheximide / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / Formation of a pool of free 40S subunits / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / translational elongation / ribosomal large subunit export from nucleus / 90S preribosome / regulation of translational fidelity / protein-RNA complex assembly / translational termination / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / translational initiation / macroautophagy / maintenance of translational fidelity / modification-dependent protein catabolic process / protein tag activity / rRNA processing / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / translation / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / : / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L1 ...60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / : / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L1 / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L44e signature. / : / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / : / Ribosomal protein L19, eukaryotic / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L30e signature 1. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein 60S L18 and 50S L18e / Ribosomal Protein L6, KOW domain / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L30e signature 2. / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / : / Ribosomal protein L6e / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L30/YlxQ / Ribosomal protein L7A/L8 / 60S ribosomal protein L6E / 60S ribosomal protein L35 / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L18e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal protein L31e, conserved site / Ribosomal L30 N-terminal domain / Ribosomal protein L31e signature. / Ribosomal protein L37ae / Ribosomal L37ae protein family / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily
Similarity search - Domain/homology
Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A ...Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL1A / Large ribosomal subunit protein uL2A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein uL11A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL8A / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL13A / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL6A / Large ribosomal subunit protein eL21A / Large ribosomal subunit protein eL13A / Large ribosomal subunit protein uL5B
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsRai J / Parker MD
Funding support United States, 3 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI)55108536 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM117093 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM086451 United States
CitationJournal: RNA / Year: 2021
Title: An open interface in the pre-80S ribosome coordinated by ribosome assembly factors Tsr1 and Dim1 enables temporal regulation of Fap7.
Authors: Jay Rai / Melissa D Parker / Haina Huang / Stefan Choy / Homa Ghalei / Matthew C Johnson / Katrin Karbstein / M Elizabeth Stroupe /
Abstract: During their maturation, nascent 40S subunits enter a translation-like quality control cycle, where they are joined by mature 60S subunits to form 80S-like ribosomes. While these assembly ...During their maturation, nascent 40S subunits enter a translation-like quality control cycle, where they are joined by mature 60S subunits to form 80S-like ribosomes. While these assembly intermediates are essential for maturation and quality control, how they form, and how their structure promotes quality control, remains unknown. To address these questions, we determined the structure of an 80S-like ribosome assembly intermediate to an overall resolution of 3.4 Å. The structure, validated by biochemical data, resolves a large body of previously paradoxical data and illustrates how assembly and translation factors cooperate to promote the formation of an interface that lacks many mature subunit contacts but is stabilized by the universally conserved methyltransferase Dim1. We also show how Tsr1 enables this interface by blocking the canonical binding of eIF5B to 40S subunits, while maintaining its binding to 60S. The structure also shows how this interface leads to unfolding of the platform, which allows for temporal regulation of the ATPase Fap7, thus linking 40S maturation to quality control during ribosome assembly.
History
DepositionApr 9, 2019-
Header (metadata) releaseMar 4, 2020-
Map releaseSep 30, 2020-
UpdateMar 20, 2024-
Current statusMar 20, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6oig
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_20077.png

Downloads & links

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Map

FileDownload / File: emd_20077.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.24 Å/pix.
x 384 pix.
= 476.16 Å		1.24 Å/pix.
x 384 pix.
= 476.16 Å		1.24 Å/pix.
x 384 pix.
= 476.16 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.24 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum-0.07800926 - 0.2322448
Average (Standard dev.)0.0012472362 (±0.01044156)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 476.16 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.241.241.24
M x/y/z384384384
origin x/y/z0.0000.0000.000
length x/y/z476.160476.160476.160
α/β/γ90.00090.00090.000
start NX/NY/NZ727265
NX/NY/NZ157157169
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS384384384
D min/max/mean-0.0780.2320.001

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Supplemental data

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Sample components

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Entire : 60S subunit of 80S-like ribosome

EntireName: 60S subunit of 80S-like ribosome
Components
  • Complex: 60S subunit of 80S-like ribosome
    • Protein or peptide: 60S ribosomal protein L1-A
    • Protein or peptide: 60S ribosomal protein L2-A
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4-A
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6-A
    • Protein or peptide: 60S ribosomal protein L7-A
    • Protein or peptide: 60S ribosomal protein L8-A
    • Protein or peptide: 60S ribosomal protein L9-A
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: 60S ribosomal protein L11-B
    • Protein or peptide: 60S ribosomal protein L12-A
    • Protein or peptide: 60S ribosomal protein L13-A
    • Protein or peptide: 60S ribosomal protein L14-A
    • Protein or peptide: 60S ribosomal protein L15-A
    • Protein or peptide: 60S ribosomal protein L16-A
    • Protein or peptide: 60S ribosomal protein L17-A
    • Protein or peptide: 60S ribosomal protein L18-A
    • Protein or peptide: 60S ribosomal protein L19-A
    • Protein or peptide: 60S ribosomal protein L20-A
    • Protein or peptide: 60S ribosomal protein L21-A
    • Protein or peptide: 60S ribosomal protein L22-A
    • Protein or peptide: 60S ribosomal protein L23-A
    • Protein or peptide: 60S ribosomal protein L24-A
    • Protein or peptide: 60S ribosomal protein L25
    • Protein or peptide: 60S ribosomal protein L26-A
    • Protein or peptide: 60S ribosomal protein L27-A
    • Protein or peptide: 60S ribosomal protein L28
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal protein L31-A
    • Protein or peptide: 60S ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L33-A
    • Protein or peptide: 60S ribosomal protein L34-A
    • Protein or peptide: 60S ribosomal protein L35-A
    • Protein or peptide: 60S ribosomal protein L36-A
    • Protein or peptide: 60S ribosomal protein L37-A
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: 60S ribosomal protein L39
    • Protein or peptide: Ubiquitin-60S ribosomal protein L40
    • Protein or peptide: 60S ribosomal protein L42-A
    • Protein or peptide: 60S ribosomal protein L43-A
    • Protein or peptide: 60S acidic ribosomal protein P0,60S acidic ribosomal protein P0,AO, L1OE
    • RNA: 25S ribosomal RNA
    • RNA: 5.8S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • Protein or peptide: P1
    • Protein or peptide: P2

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Supramolecule #1: 60S subunit of 80S-like ribosome

SupramoleculeName: 60S subunit of 80S-like ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#48
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: 60S ribosomal protein L1-A

MacromoleculeName: 60S ribosomal protein L1-A / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 22.974033 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: VKELLKYSNE TKKRNFLETV ELQVGLKNYD PQRDKRFSGS LKLPNCPRPN MSICIFGDAF DVDRAKSCGV DAMSVDDLKK LNKNKKLIK KLSKKYNAFI ASEVLIKQVP RLLGPQLSKA GKFPTPVSHN DDLYGKVTDV RSTIKFQLKK VLCLAVAVGN V EMEEDVLV ...String:
VKELLKYSNE TKKRNFLETV ELQVGLKNYD PQRDKRFSGS LKLPNCPRPN MSICIFGDAF DVDRAKSCGV DAMSVDDLKK LNKNKKLIK KLSKKYNAFI ASEVLIKQVP RLLGPQLSKA GKFPTPVSHN DDLYGKVTDV RSTIKFQLKK VLCLAVAVGN V EMEEDVLV NQILMSVNFF VSLLKKNWQN VGSLVVKSSM GPAFRL

UniProtKB: Large ribosomal subunit protein uL1A

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Macromolecule #2: 60S ribosomal protein L2-A

MacromoleculeName: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 27.217289 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: GRVIRNQRKG AGSIFTSHTR LRQGAAKLRT LDYAERHGYI RGIVKQIVHD SGRGAPLAKV VFRDPYKYRL REEIFIANEG VHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS D ARGVIGVI ...String:
GRVIRNQRKG AGSIFTSHTR LRQGAAKLRT LDYAERHGYI RGIVKQIVHD SGRGAPLAKV VFRDPYKYRL REEIFIANEG VHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS D ARGVIGVI AGGGRVDKPL LKAGRAFHKY RLKRNSWPKT RGVAMNPVDH PHGGGNHQHI GKASTISRGA VSGQKAGLIA AR RTGLLRG SQKTQ

UniProtKB: Large ribosomal subunit protein uL2A

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Macromolecule #3: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 43.719602 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: SHRKYEAPRH GHLGFLPRKR AASIRARVKA FPKDDRSKPV ALTSFLGYKA GMTTIVRDLD RPGSKFHKRE VVEAVTVVDT PPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV L VHTQIRKT ...String:
SHRKYEAPRH GHLGFLPRKR AASIRARVKA FPKDDRSKPV ALTSFLGYKA GMTTIVRDLD RPGSKFHKRE VVEAVTVVDT PPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV L VHTQIRKT PLAQKKAHLA EIQLNGGSIS EKVDWAREHF EKTVAVDSVF EQNEMIDAIA VTKGHGFEGV THRWGTKKLP RK THRGLRK VACIGAWHPA HVMWSVARAG QRGYHSRTSI NHKIYRVGKG DDEANGATSF DRTKKTITPM GGFVHYGEIK NDF IMVKGC IPGNRKRIVT LRKSLYTNTS RKALEEVSLK WIDTASKFGK GRFQTPAEKH AFMGTLKKDL

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #4: 60S ribosomal protein L4-A

MacromoleculeName: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 39.027926 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: SRPQVTVHSL TGEATANALP LPAVFSAPIR PDIVHTVFTS VNKNKRQAYA VSEKAGHQTS AESWGTGRAV ARIPRVGGGG TGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ K TKEAVAAL ...String:
SRPQVTVHSL TGEATANALP LPAVFSAPIR PDIVHTVFTS VNKNKRQAYA VSEKAGHQTS AESWGTGRAV ARIPRVGGGG TGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ K TKEAVAAL KAVGAHSDLL KVLKSKKLRA GKGKYRNRRW TQRRGPLVVY AEDNGIVKAL RNVPGVETAN VASLNLLQLA PG AHLGRFV IWTEAAFTKL DQVWGSETVA SSKVGYTLPS HIISTSDVTR IINSSEIQSA IRPAGQATQK RTHVLKKNPL KNK QVLLRL NPYAKVFAAE KLGSKKAEKT GTKPAAVFTE TLKHD

UniProtKB: Large ribosomal subunit protein uL4A

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Macromolecule #5: 60S ribosomal protein L5

MacromoleculeName: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 33.633633 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: AFQKDAKSSA YSSRFQTPFR RRREGKTDYY QRKRLVTQHK AKYNTPKYRL VVRFTNKDII CQIISSTITG DVVLAAAYSH ELPRYGITH GLTNWAAAYA TGLLIARRTL QKLGLDETYK GVEEVEGEYE LTEAVEDGPR PFKVFLDIGL QRTTTGARVF G ALKGASDG ...String:
AFQKDAKSSA YSSRFQTPFR RRREGKTDYY QRKRLVTQHK AKYNTPKYRL VVRFTNKDII CQIISSTITG DVVLAAAYSH ELPRYGITH GLTNWAAAYA TGLLIARRTL QKLGLDETYK GVEEVEGEYE LTEAVEDGPR PFKVFLDIGL QRTTTGARVF G ALKGASDG GLYVPHSENR FPGWDFETEE IDPELLRSYI FGGHVSQYME ELADDDEERF SELFKGYLAD DIDADSLEDI YT SAHEAIR ADPAFKPTEK KFTKEQYAAE SKKYRQTKLS KEERAARVAA KIAALAGQQ

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #6: 60S ribosomal protein L6-A

MacromoleculeName: 60S ribosomal protein L6-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 19.869369 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
SAQKAPKWYP SEDVAALKKT RKAARPQKLR ASLVPGTVLI LLAGRFRGKR VVYLKHLEDN TLLISGPFKV NGVPLRRVNA RYVIATSTK VSVEGVNVEK FNVEYFAKEK LTKKEKKEAN LFPEQQNKEI KAERVEDQKV VDKALIAEIK KTPLLKQYLS A SFSLKNGD KPHMLKF

UniProtKB: Large ribosomal subunit protein eL6A

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Macromolecule #7: 60S ribosomal protein L7-A

MacromoleculeName: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 25.240377 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: AEQVAAERAA RKAANKEKRA IILERNAAYQ KEYETAERNI IQAKRDAKAA GSYYVEAQHK LVFVVRIKGI NKIPPKPRKV LQLLRLTRI NSGTFVKVTK ATLELLKLIE PYVAYGYPSY STIRQLVYKR GFGKINKQRV PLSDNAIIEA NLGKYGILSI D DLIHEIIT ...String:
AEQVAAERAA RKAANKEKRA IILERNAAYQ KEYETAERNI IQAKRDAKAA GSYYVEAQHK LVFVVRIKGI NKIPPKPRKV LQLLRLTRI NSGTFVKVTK ATLELLKLIE PYVAYGYPSY STIRQLVYKR GFGKINKQRV PLSDNAIIEA NLGKYGILSI D DLIHEIIT VGPHFKQANN FLWPFKLSNP SGGWGVPRKF KHFIQGGSFG NREEFINKLV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

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Macromolecule #8: 60S ribosomal protein L8-A

MacromoleculeName: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 25.814998 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: NPLTHSTPKN FGIGQAVQPK RNLSRYVKWP EYVRVQRQKK ILSIRLKVPP TIAQFQYTLD RNTAAETFKL FNKYRPETAA EKKERLTKE AAAVAEGKSK QDASPKPYAV KYGLNHVVAL IENKKAKLVL IANDVDPIEL VVFLPALCKK MGVPYAIVKG K ARLGTLVN ...String:
NPLTHSTPKN FGIGQAVQPK RNLSRYVKWP EYVRVQRQKK ILSIRLKVPP TIAQFQYTLD RNTAAETFKL FNKYRPETAA EKKERLTKE AAAVAEGKSK QDASPKPYAV KYGLNHVVAL IENKKAKLVL IANDVDPIEL VVFLPALCKK MGVPYAIVKG K ARLGTLVN QKTSAVAALT EVRAEDEAAL AKLVSTIDAN FADKYDEVKK HWGGGILGNK AQAKMDKRAK NSDSA

UniProtKB: Large ribosomal subunit protein eL8A

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Macromolecule #9: 60S ribosomal protein L9-A

MacromoleculeName: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 21.605061 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

UniProtKB: Large ribosomal subunit protein uL6A

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Macromolecule #10: 60S ribosomal protein L10

MacromoleculeName: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 25.279271 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: ARRPARCYRY QKNKPYPKSR YNRAVPDSKI RIYDLGKKKA TVDEFPLCVH LVSNELEQLS SEALEAARIC ANKYMTTVSG RDAFHLRVR VHPFHVLRIN KMLSCAGADR LQQGMRGAWG KPHGLAARVD IGQIIFSVRT KDSNKDVVVE GLRRARYKFP G QQKIILSK ...String:
ARRPARCYRY QKNKPYPKSR YNRAVPDSKI RIYDLGKKKA TVDEFPLCVH LVSNELEQLS SEALEAARIC ANKYMTTVSG RDAFHLRVR VHPFHVLRIN KMLSCAGADR LQQGMRGAWG KPHGLAARVD IGQIIFSVRT KDSNKDVVVE GLRRARYKFP G QQKIILSK KWGFTNLDRP EYLKKREAGE VKDDGAFVKF LSKKGSLENN IREFPEYFAA QA

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #11: 60S ribosomal protein L11-B

MacromoleculeName: 60S ribosomal protein L11-B / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 19.266082 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
QNPMRDLKIE KLVLNISVGE SGDRLTRASK VLEQLSGQTP VQSKARYTVR TFGIRRNEKI AVHVTVRGPK AEEILERGLK VKEYQLRDR NFSATGNFGF GIDEHIDLGI KYDPSIGIFG MDFYVVMNRP GARVTRRKRC KGTVGNSHKT TKEDTVSWFK Q KYDADVLD K

UniProtKB: Large ribosomal subunit protein uL5B

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Macromolecule #12: 60S ribosomal protein L12-A

MacromoleculeName: 60S ribosomal protein L12-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 16.679318 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
EVKYLYLRAV GGEVGASAAL APKIGPLGLS PKKVGEDIAK ATKEFKGIKV TVQLKIQNRQ AAASVVPSAS SLVITALKEP PRDRKKDKN VKHSGNIQLD EIIEIARQMR DKSFGRTLAS VTKEILGTAQ SVGCRVDFKN PHDIIEGINA GEIEIP

UniProtKB: Large ribosomal subunit protein uL11A

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Macromolecule #13: 60S ribosomal protein L13-A

MacromoleculeName: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 21.885234 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRNARAT RAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEVK AAGLTAAYA RTIGIAVDHR RQNRNQEIFD ANVQRLKEYQ SKIIVFPRNG KAPEAEQVLS AAATFPIAQP ATDVEARAVQ D NGESAFRT ...String:
AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRNARAT RAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEVK AAGLTAAYA RTIGIAVDHR RQNRNQEIFD ANVQRLKEYQ SKIIVFPRNG KAPEAEQVLS AAATFPIAQP ATDVEARAVQ D NGESAFRT LRLARSEKKF RGIREKRARE KAEAE

UniProtKB: Large ribosomal subunit protein eL13A

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Macromolecule #14: 60S ribosomal protein L14-A

MacromoleculeName: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 14.976794 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
TDSIVKASNW RLVEVGRVVL IKKGQSAGKL AAIVEIIDQK KVLIDGPKAG VPRQAINLGQ VVLTPLTFAL PRGARTATVS KKWAAAAVC EKWAASSWAK KIAQRERRAA LTDFERFQVM VLRKQKRYTV KKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

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Macromolecule #15: 60S ribosomal protein L15-A

MacromoleculeName: 60S ribosomal protein L15-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.351162 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRAAR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVPKGATY GKPTNQGVN ELKYQRSLRA TAEERVGRRA ANLRVLNSYW VNQDSTYKYF EVILVDPQHK AIRRDARYNW ICDPVHKHRE A RGLTATGK ...String:
GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRAAR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVPKGATY GKPTNQGVN ELKYQRSLRA TAEERVGRRA ANLRVLNSYW VNQDSTYKYF EVILVDPQHK AIRRDARYNW ICDPVHKHRE A RGLTATGK KSRGINKGHK FNNTKAGRRK TWKRQNTLSL WRYRK

UniProtKB: Large ribosomal subunit protein eL15A

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Macromolecule #16: 60S ribosomal protein L16-A

MacromoleculeName: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 22.028951 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: VEPVVVIDGK GHLVGRLASV VAKQLLNGQK IVVVRAEELN ISGEFFRNKL KYHDFLRKAT AFNKTRGPFH FRAPSRIFYK ALRGMVSHK TARGKAALER LKVFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED VVAKLEAKRK V SSAEYYAK ...String:
VEPVVVIDGK GHLVGRLASV VAKQLLNGQK IVVVRAEELN ISGEFFRNKL KYHDFLRKAT AFNKTRGPFH FRAPSRIFYK ALRGMVSHK TARGKAALER LKVFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED VVAKLEAKRK V SSAEYYAK KRAFTKKVAS ANATAAESDV AKQLAALGY

UniProtKB: Large ribosomal subunit protein uL13A

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Macromolecule #17: 60S ribosomal protein L17-A

MacromoleculeName: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 20.458322 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
ARYGATSTNP AKSASARGSY LRVSFKNTRE TAQAINGWEL TKAQKYLEQV LDHQRAIPFR RFNSSIGRTA QGKEFGVTKA RWPAKSVKF VQGLLQNAAA NAEAKGLDAT KLYVSHIQVN QAPKQRRRTY RAHGRINKYE SSPSHIELVV TEKEEAVAKA A EKKVVRLT SRQRGRIAAQ KRIAA

UniProtKB: Large ribosomal subunit protein uL22A

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Macromolecule #18: 60S ribosomal protein L18-A

MacromoleculeName: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 20.478059 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
GIDHTSKQHK RSGHRTAPKS DNVYLKLLVK LYTFLARRTD APFNKVVLKA LFLSKINRPP VSVSRIARAL KQEGAANKTV VVVGTVTDD ARIFEFPKTT VAALRFTAGA RAKIVKAGGE CITLDQLAVR APKGQNTLIL RGPRNSREAV RHFGMGPHKG K APRILSTG RKFERARGRR RSKGFKV

UniProtKB: Large ribosomal subunit protein eL18A

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Macromolecule #19: 60S ribosomal protein L19-A

MacromoleculeName: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 21.631121 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
ANLRTQKRLA ASVVGVGKRK VWLDPNETSE IAQANSRNAI RKLVKNGTIV KKAVTVHSKS RTRAHAQSKR EGRHSGYGKR KGTREARLP SQVVWIRRLR VLRRLLAKYR DAGKIDKHLY HVLYKESKGN AFKHKRALVE HIIQAKADAQ REKALNEEAE A RRLKNRAA RDRRAQRVAE KRDALLKEDA

UniProtKB: Large ribosomal subunit protein eL19A

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Macromolecule #20: 60S ribosomal protein L20-A

MacromoleculeName: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 20.478852 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
MAHFKEYQVI GRRLPTESVP EPKLFRMRIF ASNEVIAKSR YWYFLQKLHK VKKASGEIVS INQINEAHPT KVKNFGVWVR YDSRSGTHN MYKEIRDVSR VAAVETLYQD MAARHRARFR SIHILKVAEI EKTADVKRQY VKQFLTKDLK FPLPHRVQKS T KTFSYKRP STFY

UniProtKB: Large ribosomal subunit protein eL20A

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Macromolecule #21: 60S ribosomal protein L21-A

MacromoleculeName: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 18.148066 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
GKSHGYRSRT RYMFQRDFRK HGAVHLSTYL KVYKVGDIVD IKANGSIQKG MPHKFYQGKT GVVYNVTKSS VGVIINKMVG NRYLEKRLN LRVEHIKHSK CRQEFLERVK ANAAKRAEAK AQGVAVQLKR QPAQPRESRI VSTEGNVPQT LAPVPYETFI

UniProtKB: Large ribosomal subunit protein eL21A

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Macromolecule #22: 60S ribosomal protein L22-A

MacromoleculeName: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 11.263863 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
QKIAKTFTVD VSSPTENGVF DPASYAKYLI DHIKVEGAVG NLGNAVTVTE DGTVVTVVST AKFSGKYLKY LTKKYLKKNQ LRDWIRFVS TKTNEYRLAF Y

UniProtKB: Large ribosomal subunit protein eL22A

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Macromolecule #23: 60S ribosomal protein L23-A

MacromoleculeName: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 14.362755 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
SGNGAQGTKF RISLGLPVGA IMNCADNSGA RNLYIIAVKG SGSRLNRLPA ASLGDMVMAT VKKGKPELRK KVMPAIVVRQ AKSWRRRDG VFLYFEDNAG VIANPKGEMK GSAITGPVGK ECADLWPRVA SNSGVVV

UniProtKB: Large ribosomal subunit protein uL14A

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Macromolecule #24: 60S ribosomal protein L24-A

MacromoleculeName: 60S ribosomal protein L24-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 15.506229 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITEEVAKK RSRKTVKAQR PITGASLDL IKERRSLKPE VRKANREEKL KANKEKKKAE KAARKAEKAK SAGTQS

UniProtKB: Large ribosomal subunit protein eL24A

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Macromolecule #25: 60S ribosomal protein L25

MacromoleculeName: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 13.771176 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
KALKVRTSAT FRLPKTLKLA RAPKYASKAV PHYNRLDSYK VIEQPITSET AMKKVEDGNI LVFQVSMKAN KYQIKKAVKE LYEVDVLKV NTLVRPNGTK KAYVRLTADY DALDIANRIG YI

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #26: 60S ribosomal protein L26-A

MacromoleculeName: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 14.134588 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
AKQSLDVSSD RRKARKAYFT APSSQRRVLL SAPLSKELRA QYGIKALPIR RDDEVLVVRG SKKGQEGKIS SVYRLKFAVQ VDKVTKEKV NGASVPINLH PSKLVITKLH LDKDRKALIQ RKGGKLE

UniProtKB: Large ribosomal subunit protein uL24A

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Macromolecule #27: 60S ribosomal protein L27-A

MacromoleculeName: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 15.437166 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
AKFLKAGKVA VVVRGRYAGK KVVIVKPHDE GSKSHPFGHA LVAGIERYPL KVTKKHGAKK VAKRTKIKPF IKVVNYNHLL PTRYTLDVE AFKSVVSTET FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF

UniProtKB: Large ribosomal subunit protein eL27A

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Macromolecule #28: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 16.630471 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
PSRFTKTRKH RGHVSAGKGR IGKHRKHPGG RGMAGGQHHH RINMDKYHPG YFGKVGMRYF HKQQAHFWKP VLNLDKLWTL IPEDKRDQY LKSASKETAP VIDTLAAGYG KILGKGRIPN VPVIVKARFV SKLAEEKIRA AGGVVELIA

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #29: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 6.560688 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
AKSKNHTAHN QTRKAHRNGI KKPKTYKYPS LKGVDPKFRR NHKHALHGTA KALAAAKK

UniProtKB: Large ribosomal subunit protein eL29

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Macromolecule #30: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 10.558344 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
SINQKLALVI KSGKYTLGYK STVKSLRQGK SKLIIIAANT PVLRKSELEY YAMLSKTKVY YFQGGNNELG TAVGKLFRVG VVSILEAGD SDILTTLA

UniProtKB: Large ribosomal subunit protein eL30

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Macromolecule #31: 60S ribosomal protein L31-A

MacromoleculeName: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 12.649754 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
LKDVVTREYT INLHKRLHGV SFKKRAPRAV KEIKKFAKLH MGTDDVRLAP ELNQAIWKRG VKGVEYRLRL RISRKRNEEE DAKNPLFSY VEPVLVASAK GLQTVVVEED

UniProtKB: Large ribosomal subunit protein eL31A

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Macromolecule #32: 60S ribosomal protein L32

MacromoleculeName: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 14.478054 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
ASLPHPKIVK KHTKKFKRHH SDRYHRVAEN WRKQKGIDSV VRRRFRGNIS QPKIGYGSNK KTKFLSPSGH KTFLVANVKD LETLTMHTK TYAAEIAHNI SAKNRVVILA RAKALGIKVT NPKGRLAL

UniProtKB: Large ribosomal subunit protein eL32

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Macromolecule #33: 60S ribosomal protein L33-A

MacromoleculeName: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 12.045935 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
AESHRLYVKG KHLSYQRSKR VNNPNVSLIK IEGVATPQDA QFYLGKRIAY VYRASKEVRG SKIRVMWGKV TRTHGNSGVV RATFRNNLP AKTFGASVRI FLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

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Macromolecule #34: 60S ribosomal protein L34-A

MacromoleculeName: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 12.608831 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
AQRVTFRRRN PYNTRSNKIK VVKTPGGILR AQHVKKLATR PKCGDCGSAL QGISTLRPRQ YATVSKTHKT VSRAYGGSRC ANCVKERII RAFLIEEQKI VKKVVKEQTE AAK

UniProtKB: Large ribosomal subunit protein eL34A

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Macromolecule #35: 60S ribosomal protein L35-A

MacromoleculeName: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 13.811444 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
AGVKAYELRT KSKEQLASQL VDLKKELAEL KVQKLSRPSL PKIKTVRKSI ACVLTVINEQ QREAVRQLYK GKKYQPKDLR AKKTRALRR ALTKFEASQV TEKQRKKQIA FPQRKYAIKA

UniProtKB: Large ribosomal subunit protein uL29A

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Macromolecule #36: 60S ribosomal protein L36-A

MacromoleculeName: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 11.020063 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
TVKTGIAIGL NKGKKVTSMT PAPKISYKKG AASNRTKFVR SLVREIAGLS PYERRLIDLI RNSGEKRARK VAKKRLGSFT RAKAKVEEM NNIIAASRRH

UniProtKB: Large ribosomal subunit protein eL36A

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Macromolecule #37: 60S ribosomal protein L37-A

MacromoleculeName: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 9.746199 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
GKGTPSFGKR HNKSHTLCNR CGRRSFHVQK KTCSSCGYPA AKTRSYNWGA KAKRRHTTGT GRMRYLKHVS RRFKNGFQTG SASKASA

UniProtKB: Large ribosomal subunit protein eL37A

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Macromolecule #38: 60S ribosomal protein L38

MacromoleculeName: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 8.714363 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
AREITDIKQF LELTRRADVK TATVKINKKL NKAGKPFRQT KFKVRGSSSL YTLVINDAGK AKKLIQSLPP TLKVNRL

UniProtKB: Large ribosomal subunit protein eL38

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Macromolecule #39: 60S ribosomal protein L39

MacromoleculeName: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 6.227444 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
AAQKSFRIKQ KMAKAKKQNR PLPQWIRLRT NNTIRYNAKR RNWRRTKMNI

UniProtKB: Large ribosomal subunit protein eL39

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Macromolecule #40: Ubiquitin-60S ribosomal protein L40

MacromoleculeName: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 6.032321 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
IIEPSLKALA SKYNCDKSVC RKCYARLPPR ATNCRKRKCG HTNQLRPKKK LK

UniProtKB: Ubiquitin-ribosomal protein eL40A fusion protein

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Macromolecule #41: 60S ribosomal protein L42-A

MacromoleculeName: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 12.115462 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
VNVPKTRKTY CKGKTCRKHT QHKVTQYKAG KASLFAQGKR RYDRKQSGFG GQTKPVFHKK AKTTKKVVLR LECVKCKTRA QLTLKRCKH FELGGEKKQK GQALQF

UniProtKB: Large ribosomal subunit protein eL42A

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Macromolecule #42: 60S ribosomal protein L43-A

MacromoleculeName: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 9.981756 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
AKRTKKVGIT GKYGVRYGSS LRRQVKKLEI QQHARYDCSF CGKKTVKRGA AGIWTCSCCK KTVAGGAYTV STAAAATVRS TIRRLREMV EA

UniProtKB: Large ribosomal subunit protein eL43A

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Macromolecule #43: 60S acidic ribosomal protein P0,60S acidic ribosomal protein P0,A...

MacromoleculeName: 60S acidic ribosomal protein P0,60S acidic ribosomal protein P0,AO, L1OE
type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 23.585346 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: GIREKKAEYF AKLREYLEEY KSLFVVGVDN VSSQQMHEVR KELRGRAVVL MGKNTMVRRA IRGFLSDLPD FEKLLPFVKG NVGFVFTNE PLTEIKNVIV SNRVAAPARA GAVAPEDIWV RAVNTGMEPG KTSFFQALGV PTKIARGTIE IVSDVKVVDA G NKVGQSEA ...String:
GIREKKAEYF AKLREYLEEY KSLFVVGVDN VSSQQMHEVR KELRGRAVVL MGKNTMVRRA IRGFLSDLPD FEKLLPFVKG NVGFVFTNE PLTEIKNVIV SNRVAAPARA GAVAPEDIWV RAVNTGMEPG KTSFFQALGV PTKIARGTIE IVSDVKVVDA G NKVGQSEA SLLNLLNISP FTFGLTVVQV YDNGQVFP(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

UniProtKB: Large ribosomal subunit protein uL10

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Macromolecule #47: P1

MacromoleculeName: P1 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 4.017944 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #48: P2

MacromoleculeName: P2 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 3.932839 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #44: 25S ribosomal RNA

MacromoleculeName: 25S ribosomal RNA / type: rna / ID: 44 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 1.096842375 MDa
SequenceString: UUGACCUCAA AUCAGGUAGG AGUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACG GCGAGUGAAG CGGCAAAAGC UCAAAUUUGA AAUCUGGUAC CUUCGGUGCC CGAGUUGUAA UUUGGAGAGG G CAACUUUG ...String:
UUGACCUCAA AUCAGGUAGG AGUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACG GCGAGUGAAG CGGCAAAAGC UCAAAUUUGA AAUCUGGUAC CUUCGGUGCC CGAGUUGUAA UUUGGAGAGG G CAACUUUG GGGCCGUUCC UUGUCUAUGU UCCUUGGAAC AGGACGUCAU AGAGGGUGAG AAUCCCGUGU GGCGAGGAGU GC GGUUCUU UGUAAAGUGC CUUCGAAGAG UCGAGUUGUU UGGGAAUGCA GCUCUAAGUG GGUGGUAAAU UCCAUCUAAA GCU AAAUAU UGGCGAGAGA CCGAUAGCGA ACAAGUACAG UGAUGGAAAG AUGAAAAGAA CUUUGAAAAG AGAGUGAAAA AGUA CGUGA AAUUGUUGAA AGGGAAGGGC AUUUGAUCAG ACAUGGUGUU UUGUGCCCUC UGCUCCUUGU GGGUAGGGGA AUCUC GCAU UUCACUGGGC CAGCAUCAGU UUUGGUGGCA GGAUAAAUCC AUAGGAAUGU AGCUUGCCUC GGUAAGUAUU AUAGCC UGU GGGAAUACUG CCAGCUGGGA CUGAGGACUG CGACGUAAGU CAAGGAUGCU GGCAUAAUGG UUAUAUGCCG CCCGUCU UG AAACACGGAC CAAGGAGUCU AACGUCUAUG CGAGUGUUUG GGUGUAAAAC CCAUACGCGU AAUGAAAGUG AACGUAGG U UGGGGCCUCG CAAGAGGUGC ACAAUCGACC GAUCCUGAUG UCUUCGGAUG GAUUUGAGUA AGAGCAUAGC UGUUGGGAC CCGAAAGAUG GUGAACUAUG CCUGAAUAGG GUGAAGCCAG AGGAAACUCU GGUGGAGGCU CGUAGCGGUU CUGACGUGCA AAUCGAUCG UCGAAUUUGG GUAUAGGGGC GAAAGACUAA UCGAACCAUC UAGUAGCUGG UUCCUGCCGA AGUUUCCCUC A GGAUAGCA GAAGCUCGUA UCAGUUUUAU GAGGUAAAGC GAAUGAUUAG AGGUUCCGGG GUCGAAAUGA CCUUGACCUA UU CUCAAAC UUUAAAUAUG UAAGAAGUCC UUGUUACUUA AUUGAACGUG GACAUUUGAA UGAAGAGCUU UUAGUGGGCC AUU UUUGGU AAGCAGAACU GGCGAUGCGG GAUGAACCGA ACGUAGAGUU AAGGUGCCGG AAUACACGCU CAUCAGACAC CACA AAAGG UGUUAGUUCA UCUAGACAGC CGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAGGAGUGU GUAACAACUC ACCGG CCGA AUGAACUAGC CCUGAAAAUG GAUGGCGCUC AAGCGUGUUA CCUAUACUCU ACCGUCAGGG UUGAUAUGAU GCCCUG ACG AGUAGGCAGG CGUGGAGGUC AGUGACGAAG CCUAGACCGU AAGGUCGGGU CGAACGGCCU CUAGUGCAGA UCUUGGU GG UAGUAGCAAA UAUUCAAAUG AGAACUUUGA AGACUGAAGU GGGGAAAGGU UCCACGUCAA CAGCAGUUGG ACGUGGGU U AGUCGAUCCU AAGAGAUGGG GAAGCUCCGU UUCAAAGGCC UGAUUUUAUG CAGGCCACCA UCGAAAGGGA AUCCGGUUA AGAUUCCGGA ACCUGGAUAU GGAUUCUUCA CGGUAACGUA ACUGAAUGUG GAGACGUCGG CGCGAGCCCU GGGAGGAGUU AUCUUUUCU UCUUAACAGC UUAUCACCCC GGAAUUGGUU UAUCCGGAGA UGGGGUCUUA UGGCUGGAAG AGGCCAGCAC C UUUGCUGG CUCCGGUGCG CUUGUGACGG CCCGUGAAAA UCCACAGGAA GGAAUAGUUU UCAUGCCAGG UCGUACUGAU AA CCGCAGC AGGUCUCCAA GGUGAACAGC CUCUAGUUGA UAGAAUAAUG UAGAUAAGGG AAGUCGGCAA AAUAGAUCCG UAA CUUCGG GAUAAGGAUU GGCUCUAAGG GUCGGGUAGU GAGGGCCUUG GUCAGACGCA GCGGGCGUGC UUGUGGACUG CUUG GUGGG GCUUGCUCUG CUAGGCGGAC UACUUGCGUG CCUUGUUGUA GACGGCCUUG GUAGGUCUCU UGUAGACCGU CGCUU GCUA CAAUUAACGA UCAACUUAGA ACUGGUACGG ACAAGGGGAA UCUGACUGUC UAAUUAAAAC AUAGCAUUGC GAUGGU CAG AAAGUGAUGU UGACGCAAUG UGAUUUCUGC CCAGUGCUCU GAAUGUCAAA GUGAAGAAAU UCAACCAAGC GCGGGUA AA CGGCGGGAGU AACUAUGACU CUCUUAAGGU AGCCAAAUGC CUCGUCAUCU AAUUAGUGAC GCGCAUGAAU GGAUUAAC G AGAUUCCCAC UGUCCCUAUC UACUAUCUAG CGAAACCACA GCCAAGGGAA CGGGCUUGGC AGAAUCAGCG GGGAAAGAA GACCCUGUUG AGCUUGACUC UAGUUUGACA UUGUGAAGAG ACAUAGAGGG UGUAGAAUAA GUGGGAGCUU CGGCGCCAGU GAAAUACCA CUACCUUUAU AGUUUCUUUA CUUAUUCAAU GAAGCGGAGC UGGAAUUCAU UUUCCACGUU CUAGCAUUCA A GGUCCCAU UCGGGGCUGA UCCGGGUUGA AGACAUUGUC AGGUGGGGAG UUUGGCUGGG GCGGCACAUC UGUUAAACGA UA ACGCAGA UGUCCUAAGG GGGGCUCAUG GAGAACAGAA AUCUCCAGUA GAACAAAAGG GUAAAAGCCC CCUUGAUUUU GAU UUUCAG UGUGAAUACA AACCAUGAAA GUGUGGCCUA UCGAUCCUUU AGUCCCUCGG AAUUUGAGGC UAGAGGUGCC AGAA AAGUU ACCACAGGGA UAACUGGCUU GUGGCAGUCA AGCGUUCAUA GCGACAUUGC UUUUUGAUUC UUCGAUGUCG GCUCU UCCU AUCAUACCGA AGCAGAAUUC GGUAAGCGUU GGAUUGUUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGU CGU GAGACAGGUU AGUUUUACCC UACUGAUGAA UGUUACCGCA AUAGUAAUUG AACUUAGUAC GAGAGGAACA GUUCAUU CG GAUAAUUGGU UUUUGCGGCU GUCUGAUCAG GCAUUGCCGC GAAGCUACCA UCCGCUGGAU UAUGGCUGAA CGCCUCUA A GUCAGAAUCC AUGCUAGAAC GCGGUGAUUU CUUUGCUCCA CACAAUAUAG AUGGAUACGA AUAAGGCGUC CUUGUGGCG UCGCUGAACC AUAGCAGGCU AGCAACGGUG CACUUGGCGG AAAGGCCUUG GGUGCUUGCU GGCGAAUUGC AAUGUCAUUU UGCGUGGGG AUAAAUCAUU UGUAUACGAC UUAGAUGUAC AACGGGGUAU UGUAAGCAGU AGAGUAGCCU UGUUGUUACG A UCUGCUGA GAUUAAGCCU UUGUUGUCUG AUUUGU

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Macromolecule #45: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 45 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 50.682922 KDa
SequenceString:
AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: MG214542.1

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Macromolecule #46: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 46 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 38.951105 KDa
SequenceString:
GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

GENBANK: GENBANK: CP008434.1

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 6.8
GridDetails: unspecified
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: DIRECT ELECTRON DE-64 (8k x 8k) / Detector mode: COUNTING / Average electron dose: 25.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: EMDB MAP
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 83558
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: MAXIMUM LIKELIHOOD

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