- PDB-6oig: Subunit joining exposes nascent pre-40S rRNA for processing and q... -
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Basic information
Entry
Database: PDB / ID: 6oig
Title
Subunit joining exposes nascent pre-40S rRNA for processing and quality control
Components
(60S ribosomal protein ...) x 41
25S ribosomal RNA
5.8S ribosomal RNA
5S ribosomal RNA
60S acidic ribosomal protein P0,60S acidic ribosomal protein P0,AO, L1OE
P1
P2
Ubiquitin-60S ribosomal protein L40
Keywords
RIBOSOME / 80S-like complex / 60S
Function / homology
Function and homology information
pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome ...pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / protein-RNA complex assembly / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / ribosomal large subunit export from nucleus / regulation of translational fidelity / translational termination / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / maintenance of translational fidelity / macroautophagy / modification-dependent protein catabolic process / protein tag activity / ribosomal large subunit assembly / rRNA processing / large ribosomal subunit rRNA binding / ribosome biogenesis / cytoplasmic translation / 5S rRNA binding / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / translation / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / RNA binding / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function
60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Ribosomal protein L29e / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal protein L27e, conserved site / Ribosomal L29e protein family ...60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Ribosomal protein L29e / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal protein L27e, conserved site / Ribosomal L29e protein family / : / Ribosomal protein L24e, conserved site / Ribosomal protein L34e, conserved site / Ribosomal protein L1, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L23/L25, N-terminal / 60S ribosomal protein L35 / Ribosomal protein L1 / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal protein L23, N-terminal domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal L27e protein family / Ribosomal Protein L6, KOW domain / Ribosomal protein L30/YlxQ / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L34e / Ribosomal protein L31e, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L37ae / Ribosomal protein L14e domain / 60S ribosomal protein L6E / Ribosomal protein L19, eukaryotic / Ribosomal protein L35A / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal L40e family / Ribosomal protein L36e / Ribosomal protein L35A superfamily / Ribosomal protein L44e signature. / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L7A/L8 / Ribosomal protein L27e signature. / Ribosomal protein L10e signature. / Ribosomal protein L32e, conserved site / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L7, eukaryotic / Ribosomal protein L6e / Ribosomal protein L14 / Ribosomal protein L30, N-terminal / Ribosomal protein L6, conserved site-2 / Ribosomal protein L14, KOW motif / Ribosomal L30 N-terminal domain / Ribosomal protein L19/L19e conserved site / Ribosomal protein L14 / Ribosomal L37ae protein family / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L35Ae / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L39e, conserved site / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L19e signature. / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L34e signature. / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal protein L6e signature. / Ribosomal_L31e / Ribosomal protein L15e, conserved site / Ribosomal protein L30e signature 1. / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L30e signature 2. / 60S ribosomal protein L19 / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L37e, conserved site / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type Similarity search - Domain/homology
: / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 ...: / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL1A / Large ribosomal subunit protein uL2A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein uL11A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL8A / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL13A / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL6A / Large ribosomal subunit protein eL21A / Large ribosomal subunit protein eL13A / Large ribosomal subunit protein uL5B Similarity search - Component
Biological species
Saccharomyces cerevisiae (brewer's yeast)
Method
ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.8 Å
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01-GM117093
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01-GM086451
United States
Citation
Journal: RNA / Year: 2021 Title: An open interface in the pre-80S ribosome coordinated by ribosome assembly factors Tsr1 and Dim1 enables temporal regulation of Fap7. Authors: Jay Rai / Melissa D Parker / Haina Huang / Stefan Choy / Homa Ghalei / Matthew C Johnson / Katrin Karbstein / M Elizabeth Stroupe / Abstract: During their maturation, nascent 40S subunits enter a translation-like quality control cycle, where they are joined by mature 60S subunits to form 80S-like ribosomes. While these assembly ...During their maturation, nascent 40S subunits enter a translation-like quality control cycle, where they are joined by mature 60S subunits to form 80S-like ribosomes. While these assembly intermediates are essential for maturation and quality control, how they form, and how their structure promotes quality control, remains unknown. To address these questions, we determined the structure of an 80S-like ribosome assembly intermediate to an overall resolution of 3.4 Å. The structure, validated by biochemical data, resolves a large body of previously paradoxical data and illustrates how assembly and translation factors cooperate to promote the formation of an interface that lacks many mature subunit contacts but is stabilized by the universally conserved methyltransferase Dim1. We also show how Tsr1 enables this interface by blocking the canonical binding of eIF5B to 40S subunits, while maintaining its binding to 60S. The structure also shows how this interface leads to unfolding of the platform, which allows for temporal regulation of the ATPase Fap7, thus linking 40S maturation to quality control during ribosome assembly.
60S ribosomal protein ... , 41 types, 41 molecules zABCDEFGHIJsLMNOPQRSTUVWXYZabc...
#1: Protein
60SribosomalproteinL1-A / Ribosome / L10a / Large ribosomal subunit protein uL1-A
Mass: 22974.033 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL1A, SSM1, SSM1A, YPL220W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX43
#2: Protein
60SribosomalproteinL2-A / Ribosome / L5 / Large ribosomal subunit protein uL2-A / RP8 / YL6
Mass: 27217.289 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL2A, RPL5B, YFR031C-A, YFR031BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX45
#3: Protein
60SribosomalproteinL3 / / Large ribosomal subunit protein uL3 / Maintenance of killer protein 8 / RP1 / Trichodermin ...Large ribosomal subunit protein uL3 / Maintenance of killer protein 8 / RP1 / Trichodermin resistance protein / YL1
Mass: 43719.602 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL3, MAK8, TCM1, YOR063W, YOR29-14 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P14126
#4: Protein
60SribosomalproteinL4-A / Ribosome / L2 / Large ribosomal subunit protein uL4-A / RP2 / YL2
Mass: 39027.926 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL4A, RPL2, RPL2A, YBR031W, YBR0315 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P10664
#5: Protein
60SribosomalproteinL5 / / L1 / L1a / Large ribosomal subunit protein uL18 / Ribosomal 5S RNA-binding protein / YL3
Mass: 33633.633 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL5, RPL1, RPL1A, YPL131W, LPI14W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P26321
#6: Protein
60SribosomalproteinL6-A / Ribosome / L17 / Large ribosomal subunit protein eL6-A / RP18 / YL16
Mass: 19869.369 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL6A, RPL17A, YL16A, YML073C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q02326
#7: Protein
60SribosomalproteinL7-A / Ribosome / L6 / Large ribosomal subunit protein uL30-A / RP11 / YL8
Mass: 25240.377 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL7A, RPL6A, RPL8A, YL8A, YGL076C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05737
#8: Protein
60SribosomalproteinL8-A / Ribosome / L4 / L4-2 / L7a-1 / Large ribosomal subunit protein eL8-A / Maintenance of killer protein 7 / RP6 / YL5
Mass: 25814.998 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL8A, MAK7, RPL4A, YHL033C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P17076
#9: Protein
60SribosomalproteinL9-A / Ribosome / L8 / Large ribosomal subunit protein uL6-A / RP24 / YL11
Mass: 21605.061 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL9A, RPL8A, RPL9, YGL147C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05738
#10: Protein
60SribosomalproteinL10 / / L9 / Large ribosomal subunit protein uL16 / Ubiquinol-cytochrome C reductase complex subunit VI- ...L9 / Large ribosomal subunit protein uL16 / Ubiquinol-cytochrome C reductase complex subunit VI-requiring protein
Mass: 25279.271 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL10, GRC5, QSR1, YLR075W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P41805
#11: Protein
60SribosomalproteinL11-B / Ribosome / L16 / Large ribosomal subunit protein uL5-B / RP39 / YL22
Mass: 19266.082 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL11B, RP39B, RPL16B, YGR085C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q3E757
#12: Protein
60SribosomalproteinL12-A / Ribosome / L15 / Large ribosomal subunit protein uL11-A / YL23
Mass: 16679.318 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL12A, RPL15B, YEL054C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX53
#13: Protein
60SribosomalproteinL13-A / Ribosome / Large ribosomal subunit protein eL13-A
Mass: 21885.234 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL13A, YDL082W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q12690
#14: Protein
60SribosomalproteinL14-A / Ribosome / Large ribosomal subunit protein eL14-A
Mass: 14976.794 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL14A, YKL006W, YKL153 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P36105
#15: Protein
60SribosomalproteinL15-A / Ribosome / L13 / Large ribosomal subunit protein eL15-A / RP15R / YL10 / YP18
Mass: 24351.162 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL15A, RPL10A, RPL13A, YL10A, YLR029C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05748
#16: Protein
60SribosomalproteinL16-A / Ribosome / L13a / L21 / Large ribosomal subunit protein uL13-A / RP22 / YL15
Mass: 22028.951 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL16A, RPL13, RPL21A, YIL133C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P26784
#17: Protein
60SribosomalproteinL17-A / Ribosome / L20A / Large ribosomal subunit protein uL22-A / YL17
Mass: 20458.322 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL17A, RPL17, RPL20A, YKL180W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05740
#18: Protein
60SribosomalproteinL18-A / Ribosome / Large ribosomal subunit protein eL18-A / RP28
Mass: 20478.059 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL18A, RP28A, YOL120C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX49
#19: Protein
60SribosomalproteinL19-A / Ribosome / L23 / Large ribosomal subunit protein eL19-A / RP15L / RP33 / YL14
Mass: 21631.121 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL19A, RPL23A, YL14A, YBR084C-A, YBR084BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX82
#20: Protein
60SribosomalproteinL20-A / Ribosome / L18a / Large ribosomal subunit protein eL20-A
Mass: 20478.852 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL20A, RPL18A, RPL18A2, YMR242C, YM9408.04C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX23
#21: Protein
60SribosomalproteinL21-A / Ribosome / Large ribosomal subunit protein eL21-A
Mass: 18148.066 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL21A, URP1, YBR191W, YBR1401 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q02753
#22: Protein
60SribosomalproteinL22-A / Ribosome / L1c / Large ribosomal subunit protein eL22-A / RP4 / YL31
Mass: 11263.863 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL22A, YLR061W, L2168 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05749
#23: Protein
60SribosomalproteinL23-A / Ribosome / L17a / Large ribosomal subunit protein uL14-A / YL32
Mass: 14362.755 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL23A, RPL17A, RPL17AA, YBL087C, YBL0713 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX41
#24: Protein
60SribosomalproteinL24-A / Ribosome / L30 / Large ribosomal subunit protein eL24-A / RP29 / YL21
Mass: 15506.229 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL24A, RP29, RPL30A, YGL031C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04449
#25: Protein
60SribosomalproteinL25 / / Large ribosomal subunit protein uL23 / RP16L / YL25 / YP42'
Mass: 13771.176 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL25, YOL127W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04456
#26: Protein
60SribosomalproteinL26-A / Ribosome / L33 / Large ribosomal subunit protein uL24-A / YL33
Mass: 14134.588 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL26A, RPL26, RPL33A, YLR344W, L8300.4 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05743
#27: Protein
60SribosomalproteinL27-A / Ribosome / Large ribosomal subunit protein eL27-A
Mass: 15437.166 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL27A, RPL27, YHR010W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0C2H6
#28: Protein
60SribosomalproteinL28 / / L27a / L29 / Large ribosomal subunit protein uL15 / RP44 / RP62 / YL24
Mass: 16630.471 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL28, CYH2, YGL103W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P02406
#29: Protein
60SribosomalproteinL29 / / Large ribosomal subunit protein eL29 / YL43
Mass: 6560.688 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL29, YL43, YFR032C-A, YFR032BW / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05747
#30: Protein
60SribosomalproteinL30 / / L32 / Large ribosomal subunit protein eL30 / RP73 / YL38
Mass: 10558.344 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL30, RPL32, YGL030W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P14120
#31: Protein
60SribosomalproteinL31-A / Ribosome / L34 / Large ribosomal subunit protein eL31-A / YL28
Mass: 12649.754 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL31A, RPL34, RPL34A, YDL075W, D2478 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0C2H8
#32: Protein
60SribosomalproteinL32 / / Large ribosomal subunit protein eL32
Mass: 14478.054 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL32, YBL092W, YBL0838 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38061
#33: Protein
60SribosomalproteinL33-A / Ribosome / L37 / Large ribosomal subunit protein eL33-A / RP47 / YL37
Mass: 12045.935 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL33A, RPL37A, YPL143W, LPI4W, P2625 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05744
#34: Protein
60SribosomalproteinL34-A / Ribosome / Large ribosomal subunit protein eL34-A
Mass: 12608.831 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL34A, YER056C-A, YER056BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P87262
#35: Protein
60SribosomalproteinL35-A / Ribosome / Large ribosomal subunit protein uL29-A
Mass: 13811.444 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL35A, SOS1, YDL191W, D1249 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX84
#36: Protein
60SribosomalproteinL36-A / Ribosome / L39 / Large ribosomal subunit protein eL36-A / YL39
Mass: 11020.063 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL36A, RPL39A, YMR194W, YM9646.06 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05745
#37: Protein
60SribosomalproteinL37-A / Ribosome / L43 / Large ribosomal subunit protein eL37-A / YL35 / YP55
Mass: 9746.199 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL37A, RPL35A, YLR185W, L9470.6 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P49166
#38: Protein
60SribosomalproteinL38 / / Large ribosomal subunit protein eL38
Mass: 8714.363 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL38, YLR325C, L8543.2 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P49167
#39: Protein/peptide
60SribosomalproteinL39 / / L46 / Large ribosomal subunit protein eL39 / YL40
Mass: 6227.444 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL39, RPL46, SPB2, YJL189W, J0360 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04650
#41: Protein
60SribosomalproteinL42-A / Ribosome / L41 / Large ribosomal subunit protein eL42-A / YL27 / YP44
Mass: 12115.462 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL42A, RPL41A, SCL41A, YNL162W, N1722 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX27
#42: Protein
60SribosomalproteinL43-A / Ribosome / L37a / Large ribosomal subunit protein eL43-A / YL35
Mass: 9981.756 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL43A, YPR043W, YP9499.02 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX25
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Protein , 2 types, 2 molecules mq
#40: Protein
Ubiquitin-60SribosomalproteinL40
Mass: 6032.321 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPL40A, UBI1, YIL148W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CH08
#43: Protein
60SacidicribosomalproteinP0,60SacidicribosomalproteinP0,AO, L1OE / A0 / L10e / Large ribosomal subunit protein uL10
Mass: 23585.346 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RPP0, L10E, RPA0, RPL10E, RPLA0, YLR340W, L8300.8 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05317
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RNA chain , 3 types, 3 molecules 587
#44: RNA chain
25SribosomalRNA /
Mass: 1096842.375 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Production host: Saccharomyces cerevisiae (brewer's yeast)
#45: RNA chain
5.8SribosomalRNA /
Mass: 50682.922 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Production host: Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 1267176496
#46: RNA chain
5SribosomalRNA /
Mass: 38951.105 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Production host: Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 1039024045
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Protein/peptide , 2 types, 2 molecules xy
#47: Protein/peptide
P1
Mass: 4017.944 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Production host: Saccharomyces cerevisiae (brewer's yeast)
#48: Protein/peptide
P2
Mass: 3932.839 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Production host: Saccharomyces cerevisiae (brewer's yeast)
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Experimental details
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Experiment
Experiment
Method: ELECTRON MICROSCOPY
EM experiment
Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction
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Sample preparation
Component
Name: 60S subunit of 80S-like ribosome / Type: RIBOSOME / Entity ID: all / Source: RECOMBINANT
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