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- EMDB-8369: Methicillin Resistant, Linezolid resistant Staphylococcus aureus ... -

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Basic information

Entry
Database: EMDB / ID: EMD-8369
TitleMethicillin Resistant, Linezolid resistant Staphylococcus aureus 70S ribosome (delta S145 uL3)
Map data
Sample
  • Complex: Clinical isolate Linezolid resistant MRSA 70S ribosome
    • RNA: x 4 types
    • Protein or peptide: x 38 types
  • Ligand: x 1 types
Function / homology
Function and homology information


large ribosomal subunit / small ribosomal subunit / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / cytoplasm
Similarity search - Function
Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type ...Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L27, conserved site / Ribosomal protein S6, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6 signature. / Ribosomal protein S11, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / L28p-like / Ribosomal protein L20 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein S15, bacterial-type / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal L32p protein family / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein L24 / Ribosomal protein L32p / Ribosomal protein S12, bacterial-type / Ribosomal protein L34 / Ribosomal protein L34 / Ribosomal protein L13, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / Ribosomal protein S10, conserved site / : / : / Ribosomal protein S10 / Ribosomal protein S10 signature. / Ribosomal protein S17, conserved site / Ribosomal protein L15, conserved site / Ribosomal protein L2, conserved site / Ribosomal protein L2 signature. / Ribosomal protein L10e/L16 / Ribosomal protein L10e/L16 superfamily / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S4/S9, N-terminal / Ribosomal protein S4, conserved site / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L6
Similarity search - Domain/homology
Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL33 ...Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS10 / 50S ribosomal protein L6 / 50S ribosomal protein L27 / : / : / : / : / 50S ribosomal protein L32 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL30
Similarity search - Component
Biological speciesStaphylococcus aureus (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsBelousoff MJ / Lithgow T / Eyal Z / Yonath A / Radjainia M
Funding support Australia, 1 items
OrganizationGrant numberCountry
NHMRC1092262 Australia
CitationJournal: mBio / Year: 2017
Title: Structural Basis for Linezolid Binding Site Rearrangement in the Ribosome.
Authors: Matthew J Belousoff / Zohar Eyal / Mazdak Radjainia / Tofayel Ahmed / Rebecca S Bamert / Donna Matzov / Anat Bashan / Ella Zimmerman / Satabdi Mishra / David Cameron / Hans Elmlund / Anton Y ...Authors: Matthew J Belousoff / Zohar Eyal / Mazdak Radjainia / Tofayel Ahmed / Rebecca S Bamert / Donna Matzov / Anat Bashan / Ella Zimmerman / Satabdi Mishra / David Cameron / Hans Elmlund / Anton Y Peleg / Shashi Bhushan / Trevor Lithgow / Ada Yonath /
Abstract: An unorthodox, surprising mechanism of resistance to the antibiotic linezolid was revealed by cryo-electron microscopy (cryo-EM) in the 70S ribosomes from a clinical isolate of This high-resolution ...An unorthodox, surprising mechanism of resistance to the antibiotic linezolid was revealed by cryo-electron microscopy (cryo-EM) in the 70S ribosomes from a clinical isolate of This high-resolution structural information demonstrated that a single amino acid deletion in ribosomal protein uL3 confers linezolid resistance despite being located 24 Å away from the linezolid binding pocket in the peptidyl-transferase center. The mutation induces a cascade of allosteric structural rearrangements of the rRNA that ultimately results in the alteration of the antibiotic binding site. The growing burden on human health caused by various antibiotic resistance mutations now includes prevalent resistance to last-line antimicrobial drugs such as linezolid and daptomycin. Structure-informed drug modification represents a frontier with respect to designing advanced clinical therapies, but success in this strategy requires rapid, facile means to shed light on the structural basis for drug resistance (D. Brown, Nat Rev Drug Discov 14:821-832, 2015, https://doi.org/10.1038/nrd4675). Here, detailed structural information demonstrates that a common mechanism is at play in linezolid resistance and provides a step toward the redesign of oxazolidinone antibiotics, a strategy that could thwart known mechanisms of linezolid resistance.
History
DepositionSep 6, 2016-
Header (metadata) releaseOct 12, 2016-
Map releaseMay 31, 2017-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5t7v
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_8369.png

Downloads & links

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Map

FileDownload / File: emd_8369.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.1 Å/pix.
x 320 pix.
= 352. Å		1.1 Å/pix.
x 320 pix.
= 352. Å		1.1 Å/pix.
x 320 pix.
= 352. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.1 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.05 / Movie #1: 0.05
Minimum - Maximum-0.46595314 - 0.73986906
Average (Standard dev.)0.002786672 (±0.027086237)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 352.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.11.11.1
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z352.000352.000352.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.4660.7400.003

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Supplemental data

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Sample components

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Entire : Clinical isolate Linezolid resistant MRSA 70S ribosome

EntireName: Clinical isolate Linezolid resistant MRSA 70S ribosome
Components
  • Complex: Clinical isolate Linezolid resistant MRSA 70S ribosome
    • RNA: 16S ribosomal RNA
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S8
    • RNA: 23S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • RNA: E-site tRNA
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Clinical isolate Linezolid resistant MRSA 70S ribosome

SupramoleculeName: Clinical isolate Linezolid resistant MRSA 70S ribosome
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#42
Source (natural)Organism: Staphylococcus aureus (bacteria)

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Macromolecule #1: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 500.520406 KDa
SequenceString: UGGAGAGUUU GAUCCUGGCU CAGGAUGAAC GCUGGCGGCG UGCCUAAUAC AUGCAAGUCG AGCGAACGGA CGAGAAGCUU GCUUCUCUG AUGUUAGCGG CGGACGGGUG AGUAACACGU GGAUAACCUA CCUAUAAGAC UGGGAUAACU UCGGGAAACC G GAGCUAAU ...String:
UGGAGAGUUU GAUCCUGGCU CAGGAUGAAC GCUGGCGGCG UGCCUAAUAC AUGCAAGUCG AGCGAACGGA CGAGAAGCUU GCUUCUCUG AUGUUAGCGG CGGACGGGUG AGUAACACGU GGAUAACCUA CCUAUAAGAC UGGGAUAACU UCGGGAAACC G GAGCUAAU ACCGGAUAAU AUUUUGAACC GCAUGGUUCG AAAGUGAAAG ACGGUCUUGC UGUCACUUAU AGAUGGAUCC GC GCUGCAU UAGCUAGUUG GUAAGGUAAC GGCUUACCAA GGCAACGAUG CAUAGCCGAC CUGAGAGGGU GAUCGGCCAC ACU GGAACU GAGACACGGU CCAGACUCCU ACGGGAGGCA GCAGUAGGGA AUCUUCCGCA AUGGGCGAAA GCCUGACGGA GCAA CGCCG CGUGAGUGAU GAAGGUCUUC GGAUCGUAAA ACUCUGUUAU UAGGGAAGAA CAUAUGUGUA AGUAACUGUG CACAU CUUG ACGGUACCUA AUCAGAAAGC CACGGCUAAC UACGUGCCAG CAGCCGCGGU AAUACGUAGG UGGCAAGCGU UAUCCG GAA UUAUUGGGCG UAAAGCGCGC GUAGGCGGUU UUUUAAGUCU GAUGUGAAAG CCCACGGCUC AACCGUGGAG GGUCAUU GG AAACUGGAAA ACUUGAGUGC AGAAGAGGAA AGUGGAAUUC CAUGUGUAGC GGUGAAAUGC GCAGAGAUAU GGAGGAAC A CCAGUGGCGA AGGCGACUUU CUGGUCUGUA ACUGACGCUG AUGUGCGAAA GCGUGGGGAU CAAACAGGAU UAGAUACCC UGGUAGUCCA CGCCGUAAAC GAUGAGUGCU AAGUGUUAGG GGGUUUCCGC CCCUUAGUGC UGCAGCUAAC GCAUUAAGCA CUCCGCCUG GGGAGUACGA CCGCAAGGUU GAAACUCAAA GGAAUUGACG GGGACCCGCA CAAGCGGUGG AGCAUGUGGU U UAAUUCGA AGCAACGCGA AGAACCUUAC CAAAUCUUGA CAUCCUUUGA CAACUCUAGA GAUAGAGCCU UCCCCUUCGG GG GACAAAG UGACAGGUGG UGCAUGGUUG UCGUCAGCUC GUGUCGUGAG AUGUUGGGUU AAGUCCCGCA ACGAGCGCAA CCC UUAAGC UUAGUUGCCA UCAUUAAGUU GGGCACUCUA AGUUGACUGC CGGUGACAAA CCGGAGGAAG GUGGGGAUGA CGUC AAAUC AUCAUGCCCC UUAUGAUUUG GGCUACACAC GUGCUACAAU GGACAAUACA AAGGGCAGCG AAACCGCGAG GUCAA GCAA AUCCCAUAAA GUUGUUCUCA GUUCGGAUUG UAGUCUGCAA CUCGACUACA UGAAGCUGGA AUCGCUAGUA AUCGUA GAU CAGCAUGCUA CGGUGAAUAC GUUCCCGGGU CUUGUACACA CCGCCCGUCA CACCACGAGA GUUUGUAACA CCCGAAG CC GGUGGAGUAA CCUUUUAGGA GCUAGCCGUC GAAGGUGGGA CAAAUGAUUG GGGUGAAGUC GUAACAAGGU AGCCGUAU C GGAAGGUGCG GCUGGAUCAC CUCCUU

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Macromolecule #14: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 14 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 945.427812 KDa
SequenceString: GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCGAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA ...String:
GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCGAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA GCAUAUCAGA AGGCACACCC GGAGAACUGA AACAUCUUAG UACCCGGAGG AAGAGAAAGA AAAUUCGAUU CC CUUAGUA GCGGCGAGCG AAACGGGAAG AGCCCAAACC AACAAGCUUG CUUGUUGGGG UUGUAGGACA CUCUAUACGG AGU UACAAA GGACGACAUU AGACGAAUCA UCUGGAAAGA UGAAUCAAAG AAGGUAAUAA UCCUGUAGUC GAAAAUGUUG UCUC UCUUG AGUGGAUCCU GAGUACGACG GAGCACGUGA AAUUCCGUCG GAAUCUGGGA GGACCAUCUC CUAAGGCUAA AUACU CUCU AGUGACCGAU AGUGAACCAG UACCGUGAGG GAAAGGUGAA AAGCACCCCG GAAGGGGAGU GAAAUAGAAC CUGAAA CCG UGUGCUUACA AGUAGUCAGA GCCCGUUAAU GGGUGAUGGC GUGCCUUUUG UAGAAUGAAC CGGCGAGUUA CGAUUUG AU GCAAGGUUAA GCAGUAAAUG UGGAGCCGUA GCGAAAGCGA GUCUGAAUAG GGCGUUUAGU AUUUGGUCGU AGACCCGA A ACCAGGUGAU CUACCCUUGG UCAGGUUGAA GUUCAGGUAA CACUGAAUGG AGGACCGAAC CGACUUACGU UGAAAAGUG AGCGGAUGAA CUGAGGGUAG CGGAGAAAUU CCAAUCGAAC CUGGAGAUAG CUGGUUCUCU CCGAAAUAGC UUUAGGGCUA GCCUCAAGU GAUGAUUAUU GGAGGUAGAG CACUGUUUGG ACGAGGGGCC CUCUCGGGUU ACCGAAUUCA GACAAACUCC G AAUGCCAA UUAAUUUAAC UUGGGAGUCA GAACAUGGGU GAUAAGGUCC GUGUUCGAAA GGGAAACAGC CCAGACCACC AG CUAAGGU CCCAAAAUAU AUGUUAAGUG GAAAAGGAUG UGGCGUUGCC CAGACAACUG GGAUGUUGGC UUAGAAGCAG CCA UCAUUU AAAGAGUGCG UAAUAGCUCA CUAGUCGAGU GACACUGCGC CGAAAAUGUA CCGGGGCUAA ACAUAUUACC GAAG CUGUG GAUUGUCCUU UGGACAAUGG UAGGAGAGCG UUCUAAGGGC GUUGAAGCAU GAUCGUAAGG ACAUGUGGAG CGCUU AGAA GUGAGAAUGC CGGUGUGAGU AGCGAAAGAC GGGUGAGAAU CCCGUCCACC GAUUGACUAA GGUUUCCAGA GGAAGG CUC GUCCGCUCUG GGUUAGUCGG GUCCUAAGCU GAGGCCGACA GGCGUAGGCG AUGGAUAACA GGUUGAUAUU CCUGUAC CA CCUAUAAUCG UUUUAAUCGA UGGGGGGACG CAGUAGGAUA GGCGAAGCGU GCGAUUGGAU UGCACGUCUA AGCAGUAA G GCUGAGUAUU AGGCAAAUCC GGUACUCGUU AAGGCUGAGC UGUGAUGGGG AGAAGACAUU GUGUCUUCGA GUCGUUGAU UUCACACUGC CGAGAAAAGC CUCUAGAUAG AAAAUAGGUG CCCGUACCGC AAACCGACAC AGGUAGUCAA GAUGAGAAUU CUAAGGUGA GCGAGCGAAC UCUCGUUAAG GAACUCGGCA AAAUGACCCC GUAACUUCGG GAGAAGGGGU GCUCUUUAGG G UUAACGCC CAGAAGAGCC GCAGUGAAUA GGCCCAAGCG ACUGUUUAUC AAAAACACAG GUCUCUGCUA AACCGUAAGG UG AUGUAUA GGGGCUGACG CCUGCCCAGU GCUGGAAGGU UAAGAGGAGU GGUUAGCUUC UGCGAAGCUA CGAAUCGAAG CCC CAGUAA ACGGCGGCCG UAACUAUAAC GGUCCUAAGG UAGCGAAAUU CCUUGUCGGG UAAGUUCCGA CCCGCACGAA AGGC GUAAC GAUUUGGGCA CUGUCUCAAC GAGAGACUCG GUGAAAUCAU AGUACCUGUG AAGAUGCAGG UUACCCGCGA CAGGA CGGA AAGACCCCGU GGAGCUUUAC UGUAGCCUGA UAUUGAAAUU CGGCACAGCU UGUACAGGAU AGGUAGGAGC CUUUGA AAC GUGAGCGCUA GCUUACGUGG AGGCGCUGGU GGGAUACUAC CCUAGCUGUG UUGGCUUUCU AACCCGCACC ACUUAUC GU GGUGGGAGAC AGUGUCAGGC GGGCAGUUUG ACUGGGGCGG UCGCCUCCUA AAAGGUAACG GAGGCGCUCA AAGGUUCC C UCAGAAUGGU UGGAAAUCAU UCAUAGAGUG UAAAGGCAUA AGGGAGCUUG ACUGCGAGAC CUACAAGUCG AGCAGGGUC GAAAGACGGA CUUAGUGAUC CGGUGGUUCC GCAUGGAAGG GCCAUCGCUC AACGGAUAAA AGCUACCCCG GGGAUAACAG GCUUAUCUC CCCCAAGAGU UCACAUCGAC GGGGAGGUUU GGCACCUCGA UGUCGGCUCA UCGCAUCCUG GGGCUGUAGU C GGUCCCAA GGGUUGGGCU GUUCGCCCAU UAAAGCGGUA CGCGAGCUGG GUUCAGAACG UCGUGAGACA GUUCGGUCCC UA UCCGUCG UGGGCGUAGG AAAUUUGAGA GGAGCUGUCC UUAGUACGAG AGGACCGGGA UGGACAUACC UCUGGUGUAC CAG UUGUCG UGCCAACGGC AUAGCUGGGU AGCUAUGUGU GGACGGGAUA AGUGCUGAAA GCAUCUAAGC AUGAAGCCCC CCUC AAGAU GAGAUUUCCC AACUUCGGUU AUAAGAUCCC UCAAAGAUGA UGAGGUUAAU AGGUUCGAGG UGGAAGCAUG GUGAC AUGU GGAGCUGACG AAUACUAAUC GAUCGAAGAC UUAAU

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Macromolecule #15: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 15 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 36.652777 KDa
SequenceString:
UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGCUC CUUAGCGACG AUGGUAGUCA ACUUACGUU CCGCUAGAGU AGAACGUUGC CAGGC

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Macromolecule #42: E-site tRNA

MacromoleculeName: E-site tRNA / type: rna / ID: 42 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.851152 KDa
SequenceString:
GCCCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUGAAAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCAC

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Macromolecule #2: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.214458 KDa
SequenceString:
YDHRVIDQSA EKIVETAKRS GADVSGPIPL PTEKSVYTII RAVHGYKDSR EQFEQRTHKR LIDIVNPTPK TVDALMGLNL PS

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Macromolecule #3: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.146741 KDa
SequenceString:
NIENGVAHIR STFNNTIVTI TDEFGNALSW SSAGALGFKG SKKSTPFAAQ MASETASKSA MEHGLKTVEV TVKGPGPGRE SAIRALQSA GLEVTAIRDV TPVPHNGCRP PKRRRV

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Macromolecule #4: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.131569 KDa
SequenceString:
PTINQLVRKP RQSKIKKSDS PALNKGFNSK KKKFTDLNSP QARGVCTRVG TMTPKKPNSA LRKYARVRLS NNIEINAYIP GIGHNLQEH SVVLVRGGRV KDLPGVRYHI VRGALDTSGV DGRRQGRSLY GTKKPKN

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Macromolecule #5: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.34594 KDa
SequenceString:
AISQERKNEI IKEYRVHETD TGSPEVQIAV LTAEINAVNE HLRTHKKDHH SRRGLLKMVG RRRHLLNYLR SKDIQRYREL IKSLGIR

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Macromolecule #6: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.455689 KDa
SequenceString:
AVKIRLTRLG SKRNPFYRIV VADARSPRDG RIIEQIGTYN PTSANAPEIK VDEALALKWL NDGAKPTDTV HNILSK

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Macromolecule #7: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.623185 KDa
SequenceString:
RNDRKVYVGK VVSDKMDKTI TVLVETYKTH KLYGKRVKYS KKYKTHDENN SAKLGDIVKI QETRPLSATK RFRLVEIVEE SV

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Macromolecule #8: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.533737 KDa
SequenceString:
THIDYKDTEL LKRFISERGK ILPRRVTGTS AKYQRMLTTA IKRSRHMALL PYVKE

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Macromolecule #9: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.295577 KDa
SequenceString:
KSAIKRVKTT EKAEARNISQ KSAMRTAVKN AKTAVSNNAD NKNELVSLAV KLVDKAAQSN LIHSNKADRI KSQLMT

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Macromolecule #10: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.763027 KDa
SequenceString: ARFRGSNWKK SRRLGISLSG TGKELEKRPY APGQHGPNQR KKLSEYGLQL REKQKLRYLY GMTERQFRNT FDIAGKKFGV HGENFMILL ASRLDAVVYS LGLARTRRQA RQLVNHGHIL VDGKRVDIPS YSVKPGQTIS VREKSQKLNI IVESVEINNF V PEYLNFDA ...String:
ARFRGSNWKK SRRLGISLSG TGKELEKRPY APGQHGPNQR KKLSEYGLQL REKQKLRYLY GMTERQFRNT FDIAGKKFGV HGENFMILL ASRLDAVVYS LGLARTRRQA RQLVNHGHIL VDGKRVDIPS YSVKPGQTIS VREKSQKLNI IVESVEINNF V PEYLNFDA DSLTGTFVRL PERSELPAEI NEQLIVEYYS

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Macromolecule #11: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.245786 KDa
SequenceString:
EFEERVVTIN RVAKVVKGGR RFRFTALVVV GDKNGRVGFG TGKAQEVPEA IKKAVEAAKK DLVVVPRVEG TTPHTITGRY GSGSVFMKP AAPGTGVIAG GPVRAVLELA GITDILSKSL GSNTPINMVR ATIDGLQNLK NAEDVAKLRG KTVEE

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Macromolecule #12: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.86437 KDa
SequenceString:
RTYEVMYIVR PNIEEDAKKA LVERFNGILA TEGAEVLEAK DWGKRRLAYE INDFKDGFYN IVRVKSDNNK ATDEFQRLAK ISDDIIRYM VIR

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Macromolecule #13: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 14.536908 KDa
SequenceString:
TMTDPIADML TRVRNANMVR HEKLELPASN IKKEIAEILK SEGFIKNVEY VEDDKQGVLR LFLKYGQNDE RVITGLKRIS KPGLRVYAK ASEMPKVLNG LGIALVSTSE GVITDKEARK RNVGGEIIAY V

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Macromolecule #16: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.003277 KDa
SequenceString:
NHKLIEAVTK SQLRTDLPSF RPGDTLRVHV RIIEGTRERI QVFEGVVIKR RGGGVSETFT VRKISSGVGV ERTFPLHTPK IEKIEVKRR GKVRRAKLYY LRSLRGKAAR IQEI

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Macromolecule #17: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 29.956789 KDa
SequenceString: AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG ...String:
AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG KYVLIRLRSG EVRMILSTCR ATIGQVGNLQ HELVNVGKAG RSRWKGIRPT VRGSVMNPND HPHGGGEGRA PI GRPSPMS PWGKPTLGKK TRRGKKSSDK LIVRGRKK

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Macromolecule #18: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.459919 KDa
SequenceString:
PRVKGGTVTR ARRKKTIKLA KGYFGSKHTL YKVAKQQVMK SGQYAFRDRR QRKRDFRKLW ITRINAAARQ HEMSYSRLMN GLKKAGIDI NRKMLSEIAI SDEKAFAQLV TKAKDAL

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Macromolecule #19: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.1689 KDa
SequenceString:
MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK I

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Macromolecule #20: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.199245 KDa
SequenceString:
MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GK

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Macromolecule #21: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.181837 KDa
SequenceString:
RDILKRPVIT EKSSEAMAED KYTFDVDTRV NKTQVKMAVE EIFNVKVASV NIMNYKPKKK RMGRYQGYTN KRRKAIVTLK EGSDDLF

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Macromolecule #22: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.115025 KDa
SequenceString:
HIKKGDNVKV IAGKDKGKEG KVIATLPKKD RVVVEGVNIM KKHQKPTQLN PEGGILETEA AIHVSNVQLL DPKTNEPTRV GYKFVDGKK VRIAKKSGEE IK

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Macromolecule #23: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.348097 KDa
SequenceString:
ASLKSIIRQG KQTRSDLKQL RKSGKVPAVV YGYGTKNVSV KVDEVEFIKV IREVGRNGVI ELGVGSKTIK VMVADYQFDP LKNQITHID FLAI

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Macromolecule #24: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.496557 KDa
SequenceString:
SSTKNGRDSE SKRLGAKRAD GQFVTGGSIL YRQRGTKIYP GENVGRGGDD TLFAKIDGVV KFERKGRDKK QVSVYAVA

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Macromolecule #25: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.607769 KDa
SequenceString:
GKQCFVTGRK ASTGNRRSHA LNSTKRRWNA NLQKVRILVD GKPKKVWVSA RALKSGKVT

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Macromolecule #26: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.2053 KDa
SequenceString:
MKAKEIRDLT TSEIEEQIKS SKEELFNLRF QLATGQLEET ARIRTVRKTI ARLKTVARER E

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Macromolecule #27: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.112465 KDa
SequenceString: TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGHFHRAP GSVGMASDAS R VFKGQKMP ...String:
TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGHFHRAP GSVGMASDAS R VFKGQKMP GRMGGNTVTV QNLEVVQVDT ENKVILVKGN VPGPKKGLVE IRTSIK

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Macromolecule #28: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.305308 KDa
SequenceString:
AKLQITLTRS VIGRPETQRK TVEALGLKKT NSSVVVEDNP AIRGQINKVK HLVTVEE

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Macromolecule #29: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.098078 KDa
SequenceString:
AVPKRRTSKT RKNKRRTHFK ISVPGMTECP NCGEYKLSHR VCKNCGSYNG EEV

+
Macromolecule #30: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.6555 KDa
SequenceString:
MRVNVTLACT ECGDRNYITT KNKRNNPERV EMKKFCSREN KQTLHRE

+
Macromolecule #31: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.323446 KDa
SequenceString:
VKRTYQPNKR KHSKVHGFRK RMSTKNGRKV LARRRRKGRK VLSA

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Macromolecule #32: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.461992 KDa
SequenceString:
PKMKTHRGAA KRVKRTASGQ LKRSRAFTSH LFANKSTKQK RQLRKARLVS KSDMKRVKQL LAYK

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Macromolecule #33: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 4.318422 KDa
SequenceString:
MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG

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Macromolecule #34: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.179322 KDa
SequenceString: YDVLKLDGTK SGSIELSDAV FGIEPNNSVL FEAINLQRAS LRQGTHAVKN RSAVSGGGRK PWKQKGTGRA RQGTIRAPQW RGGGIVFGP TPRSYAYKMP KKMRRLALRS ALSFKAQENG LTVVDAFNFE APKTKEFKNV LSTLEQPKKV LVVTENEDVN V ELSARNIP ...String:
YDVLKLDGTK SGSIELSDAV FGIEPNNSVL FEAINLQRAS LRQGTHAVKN RSAVSGGGRK PWKQKGTGRA RQGTIRAPQW RGGGIVFGP TPRSYAYKMP KKMRRLALRS ALSFKAQENG LTVVDAFNFE APKTKEFKNV LSTLEQPKKV LVVTENEDVN V ELSARNIP GVQVTTAQGL NVLDITNADS LVITEAAAKK VEEVLG

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Macromolecule #35: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 19.414076 KDa
SequenceString:
SRVGKKIIDI PSDVTVTFDG NHVTVKGPKG ELSRTLNERM TFKQEENTIE VVRPSESKED RTNHGTTRAL LNNMVQGVSQ GYVKVLELV GVGYRAQMQG KDLILNVGYS HPVEIKAEEN ITFSVEKNTV VKVEGISKEQ VGALASNIRS VRPPEPYKGK G IRYQGEYV RRKEGK

+
Macromolecule #36: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.17118 KDa
SequenceString:
RQTFMANESN IERKWYVIDA EGQTLGRLSS EVASILRGKN KVTYTPHVDT GDYVIVINAS KIEFTGNKET DKVYYRHSNH PGGIKSITA GELRRTNPER LIENSIKGML PSTRLGEKQG KKLFVYGGAE HPHAAQQPEN YELR

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Macromolecule #37: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.044184 KDa
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EV

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Macromolecule #38: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.384533 KDa
SequenceString:
KLHELKPAEG SRKERNRVGR GVATGNGKTS GRGHKGQKAR SGGGVRPGFE GGQLPLFRRL PKRGFTNINR KEYAIVNLDQ LNKFEDGTE VTPALLVESG VVKNEKSGIK ILGNGSLDKK LTVKAHKFSA SAAEAIDAKG GAHEV

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Macromolecule #39: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.486276 KDa
SequenceString:
MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREE

+
Macromolecule #40: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.640575 KDa
SequenceString:
GYRKLGRTSD QRKAMLRDLA TSLIISERIE TTEARAKEVR SVVEKLITLG KKGDLASRRN AAKTLRNVEI LNEDETTQTA LQKLFGEIA ERYTERQGGY TRILKQGPRR GDGAESVIIE LV

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Macromolecule #41: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.792662 KDa
SequenceString:
KIDKNKVRLK RHARVRTNLS GTAEKPRLNV YRSNKHIYAQ IIDDNKGVTL AQASSKDSDI ATTATKVELA TKVGEAIAKK AADKGIKEI VFDRGGYLYH GRVKALAEAA RESGLEF

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Macromolecule #43: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 43 / Number of copies: 17 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.3 mg/mL
BufferpH: 7.5
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 45.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3j9z

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: ANGULAR RECONSTITUTION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 80500

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-5t7v:
Methicillin Resistant, Linezolid resistant Staphylococcus aureus 70S ribosome (delta S145 uL3)

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