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- EMDB-0284: Mycobacterium smegmatis alternative protein ribosome (tRNA class 1) -
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Open data
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Basic information
Entry | Database: EMDB / ID: 0284 |
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Title | Mycobacterium smegmatis alternative protein ribosome (tRNA class 1) |
![]() | Class 1 from focused classification with signal subtraction masking the tRNA binding sites |
![]() | M.smegmatis cytosolic ribosome from Zn depleted cells binding alternative ribosomal proteins.: |
Source | ![]() ![]() |
Method | ![]() ![]() |
![]() | Tobiasson V / Amunts A |
![]() | Journal: To Be Published Title: Cryo-EM structure of Mycobacterium smegmatis ribosome from zinc depleted cells. ![]() |
Date | Deposition: Oct 10, 2018 / Header (metadata) release: Jan 23, 2019 / Map release: Jan 23, 2019 / Last update: Jan 23, 2019 |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
![]() | ![]() |
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Header (meta data in XML format) | ![]() ![]() ![]() |
Images | ![]() |
Others | ![]() ![]() |
FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-0284 |
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Links
-Related structure data
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire M.smegmatis cytosolic ribosome from Zn depleted cells binding alt...
Entire | Name: M.smegmatis cytosolic ribosome from Zn depleted cells binding alternative ribosomal proteins. Number of components: 1 |
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-Component #1: protein, M.smegmatis cytosolic ribosome from Zn depleted cells bi...
Protein | Name: M.smegmatis cytosolic ribosome from Zn depleted cells binding alternative ribosomal proteins. Recombinant expression: No |
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Source | Species: ![]() ![]() |
Source (natural) | Location in cell: Cytoplasm |
-Experimental details
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Sample preparation
Specimen | Specimen state: cell / Method: ![]() |
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Sample solution | pH: 7.4 |
Vitrification | Instrument: FEI VITROBOT MARK II / Cryogen name: ETHANE / Temperature: 277 K / Humidity: 100 % |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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![]() | Microscope: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Cs: 2.7 mm / Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: FEI TITAN KRIOS AUTOGRID HOLDER |
Camera | Detector: GATAN K2 BASE (4k x 4k) |
-Image acquisition
Image acquisition | Number of digital images: 2724 |
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Image processing
![]() | Method: ![]() |
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3D reconstruction | Resolution: 4.5 Å / Resolution method: FSC 0.143 CUT-OFF |