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- EMDB-0177: Cryo-EM structure of the ABCF protein VmlR bound to the Bacillus ... -

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Basic information

Entry
Database: EMDB / ID: EMD-0177
TitleCryo-EM structure of the ABCF protein VmlR bound to the Bacillus subtilis ribosome
Map dataCryo-EM map of 70S Bacillus subtilis ribosome in complex with the ABCF protein VmlR. Sharpened by B-factor.
Sample
  • Complex: Complex of translating 70S ribosome with ABCF antibiotic resistance protein vmlR
    • Complex: Ribosome
      • RNA: x 5 types
      • Protein or peptide: x 47 types
    • Complex: Nucleotide-binding protein ExpZ
      • Protein or peptide: x 1 types
    • Complex: tRNATransfer RNA
  • Ligand: x 2 types
Function / homology
Function and homology information


positive regulation of rRNA processing / nucleoid / rescue of stalled ribosome / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / rRNA processing / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit ...positive regulation of rRNA processing / nucleoid / rescue of stalled ribosome / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / rRNA processing / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / regulation of translation / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / transferase activity / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / response to antibiotic / mRNA binding / DNA binding / RNA binding / zinc ion binding / ATP binding / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Ribosome protection protein VmlR / ABC-transporter extension domain / ABC transporter / Ribosomal protein L1, bacterial-type / Ribosomal protein S14, type Z / Ribosomal protein L1, conserved site / Ribosomal protein L1 / Ribosomal protein L1 signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L1-like ...Ribosome protection protein VmlR / ABC-transporter extension domain / ABC transporter / Ribosomal protein L1, bacterial-type / Ribosomal protein S14, type Z / Ribosomal protein L1, conserved site / Ribosomal protein L1 / Ribosomal protein L1 signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L31 type A / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein S14/S29 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / ABC transporter / ABC transporter-like, ATP-binding domain / Ribosomal protein S13, bacterial-type / ATP-binding cassette, ABC transporter-type domain profile. / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily
Similarity search - Domain/homology
Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL5 ...Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL28 / Ribosome protection protein VmlR / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein uL1
Similarity search - Component
Biological speciesBacillus subtilis subsp. subtilis str. 168 (bacteria) / Escherichia coli (E. coli) / Bacillus subtilis (strain 168) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsCrowe-McAuliffe C / Graf M / Huter P / Abdelshahid M / Novacek J / Wilson DN
Funding support Germany, Czech Republic, 4 items
OrganizationGrant numberCountry
German Research FoundationFOR-1805 Germany
Ministry of Education (Czech Republic)CIISB project number LM2015043 Czech Republic
German Research FoundationWI3285/6-1 Germany
European UnioniNext project number 2992 Czech Republic
CitationJournal: Proc Natl Acad Sci U S A / Year: 2018
Title: Structural basis for antibiotic resistance mediated by the ABCF ATPase VmlR.
Authors: Caillan Crowe-McAuliffe / Michael Graf / Paul Huter / Hiraku Takada / Maha Abdelshahid / Jiří Nováček / Victoriia Murina / Gemma C Atkinson / Vasili Hauryliuk / Daniel N Wilson /
Abstract: Many Gram-positive pathogenic bacteria employ ribosomal protection proteins (RPPs) to confer resistance to clinically important antibiotics. In , the RPP VmlR confers resistance to lincomycin (Lnc) ...Many Gram-positive pathogenic bacteria employ ribosomal protection proteins (RPPs) to confer resistance to clinically important antibiotics. In , the RPP VmlR confers resistance to lincomycin (Lnc) and the streptogramin A (S) antibiotic virginiamycin M (VgM). VmlR is an ATP-binding cassette (ABC) protein of the F type, which, like other antibiotic resistance (ARE) ABCF proteins, is thought to bind to antibiotic-stalled ribosomes and promote dissociation of the drug from its binding site. To investigate the molecular mechanism by which VmlR confers antibiotic resistance, we have determined a cryo-electron microscopy (cryo-EM) structure of an ATPase-deficient VmlR-EQ mutant in complex with a ErmDL-stalled ribosomal complex (SRC). The structure reveals that VmlR binds within the E site of the ribosome, with the antibiotic resistance domain (ARD) reaching into the peptidyltransferase center (PTC) of the ribosome and a C-terminal extension (CTE) making contact with the small subunit (SSU). To access the PTC, VmlR induces a conformational change in the P-site tRNA, shifting the acceptor arm out of the PTC and relocating the CCA end of the P-site tRNA toward the A site. Together with microbiological analyses, our study indicates that VmlR allosterically dissociates the drug from its ribosomal binding site and exhibits specificity to dislodge VgM, Lnc, and the pleuromutilin tiamulin (Tia), but not chloramphenicol (Cam), linezolid (Lnz), nor the macrolide erythromycin (Ery).
History
Header (metadata) releaseDec 21, 2016-
DepositionAug 7, 2018-
Map releaseAug 29, 2018-
UpdateJan 27, 2021-
Current statusJan 27, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.11
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.11
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6ha8
  • Surface level: 0.11
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_0177.png

Downloads & links

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Map

FileDownload / File: emd_0177.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM map of 70S Bacillus subtilis ribosome in complex with the ABCF protein VmlR. Sharpened by B-factor.
Voxel sizeX=Y=Z: 1.061 Å
Density
Contour LevelBy AUTHOR: 0.11 / Movie #1: 0.11
Minimum - Maximum-0.37885925 - 0.6340249
Average (Standard dev.)0.002984229 (±0.027424876)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 381.96 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0611.0611.061
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z381.960381.960381.960
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-0.3790.6340.003

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Supplemental data

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Additional map: Cryo-EM map of 70S Bacillus subtilis ribosome in...

Fileemd_0177_additional.map
AnnotationCryo-EM map of 70S Bacillus subtilis ribosome in complex with the ABCF protein VmlR. Filtered by local resolution.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Complex of translating 70S ribosome with ABCF antibiotic resistan...

EntireName: Complex of translating 70S ribosome with ABCF antibiotic resistance protein vmlR
Components
  • Complex: Complex of translating 70S ribosome with ABCF antibiotic resistance protein vmlR
    • Complex: Ribosome
      • RNA: 23S rRNA23S ribosomal RNA
      • RNA: 5S rRNA5S ribosomal RNA
      • Protein or peptide: 50S ribosomal protein L2
      • Protein or peptide: 50S ribosomal protein L3
      • Protein or peptide: 50S ribosomal protein L4
      • Protein or peptide: 50S ribosomal protein L5
      • Protein or peptide: 50S ribosomal protein L6
      • Protein or peptide: 50S ribosomal protein L13
      • Protein or peptide: 50S ribosomal protein L14
      • Protein or peptide: 50S ribosomal protein L15
      • Protein or peptide: 50S ribosomal protein L16
      • Protein or peptide: 50S ribosomal protein L17
      • Protein or peptide: 50S ribosomal protein L18
      • Protein or peptide: 50S ribosomal protein L19
      • Protein or peptide: 50S ribosomal protein L20
      • Protein or peptide: 50S ribosomal protein L21
      • Protein or peptide: 50S ribosomal protein L22
      • Protein or peptide: 50S ribosomal protein L23
      • Protein or peptide: 50S ribosomal protein L24
      • Protein or peptide: 50S ribosomal protein L27
      • Protein or peptide: 50S ribosomal protein L28
      • Protein or peptide: 50S ribosomal protein L29
      • Protein or peptide: 50S ribosomal protein L30
      • Protein or peptide: 50S ribosomal protein L32
      • Protein or peptide: 50S ribosomal protein L33 1Ribosome
      • Protein or peptide: 50S ribosomal protein L34
      • Protein or peptide: 50S ribosomal protein L35
      • Protein or peptide: 50S ribosomal protein L36
      • Protein or peptide: 50S ribosomal protein L31
      • RNA: mRNAMessenger RNA
      • Protein or peptide: 50S ribosomal protein L1
      • RNA: 16S rRNA
      • Protein or peptide: 30S ribosomal protein S2
      • Protein or peptide: 30S ribosomal protein S3
      • Protein or peptide: 30S ribosomal protein S4
      • Protein or peptide: 30S ribosomal protein S5
      • Protein or peptide: 30S ribosomal protein S6
      • Protein or peptide: 30S ribosomal protein S7
      • Protein or peptide: 30S ribosomal protein S8
      • Protein or peptide: 30S ribosomal protein S9
      • Protein or peptide: 30S ribosomal protein S10
      • Protein or peptide: 30S ribosomal protein S11
      • Protein or peptide: 30S ribosomal protein S12
      • Protein or peptide: 30S ribosomal protein S13
      • Protein or peptide: 30S ribosomal protein S14
      • Protein or peptide: 30S ribosomal protein S15
      • Protein or peptide: 30S ribosomal protein S16
      • Protein or peptide: 30S ribosomal protein S17
      • Protein or peptide: 30S ribosomal protein S18
      • Protein or peptide: 30S ribosomal protein S19
      • Protein or peptide: 30S ribosomal protein S20
      • RNA: P-tRNA(Leu)
    • Complex: Nucleotide-binding protein ExpZ
      • Protein or peptide: Nucleotide-binding protein ExpZ
    • Complex: tRNATransfer RNA
  • Ligand: TELITHROMYCIN
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE

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Supramolecule #1: Complex of translating 70S ribosome with ABCF antibiotic resistan...

SupramoleculeName: Complex of translating 70S ribosome with ABCF antibiotic resistance protein vmlR
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53

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Supramolecule #2: Ribosome

SupramoleculeName: Ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#19, #21-#53
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)

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Supramolecule #3: Nucleotide-binding protein ExpZ

SupramoleculeName: Nucleotide-binding protein ExpZ / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #20
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)

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Supramolecule #4: tRNA

SupramoleculeName: tRNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #53
Source (natural)Organism: Escherichia coli (E. coli)

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Macromolecule #1: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 949.646312 KDa
SequenceString: GGUUAAGUUA GAAAGGGCGC ACGGUGGAUG CCUUGGCACU AGGAGCCGAU GAAGGACGGG ACGAACACCG AUAUGCUUCG GGGAGCUGU AAGCAAGCUU UGAUCCGGAG AUUUCCGAAU GGGGAAACCC ACCACUCGUA AUGGAGUGGU AUCCAUAUCU G AAUUCAUA ...String:
GGUUAAGUUA GAAAGGGCGC ACGGUGGAUG CCUUGGCACU AGGAGCCGAU GAAGGACGGG ACGAACACCG AUAUGCUUCG GGGAGCUGU AAGCAAGCUU UGAUCCGGAG AUUUCCGAAU GGGGAAACCC ACCACUCGUA AUGGAGUGGU AUCCAUAUCU G AAUUCAUA GGAUAUGAGA AGGCAGACCC GGGGAACUGA AACAUCUAAG UACCCGGAGG AAGAGAAAGC AAAUGCGAUU CC CUGAGUA GCGGCGAGCG AAACGGGAUC AGCCCAAACC AAGAGGCUUG CCUCUUGGGG UUGUAGGACA CUCUGUACGG AGU UACAAA GGAACGAGGU AGAUGAAGAG GUCUGGAAAG GCCCGCCAUA GGAGGUAACA GCCCUGUAGU CAAAACUUCG UUCU CUCCU GAGUGGAUCC UGAGUACGGC GGAACACGUG AAAUUCCGUC GGAAUCCGGG AGGACCAUCU CCCAAGGCUA AAUAC UCCC UAGUGACCGA UAGUGAACCA GUACCGUGAG GGAAAGGUGA AAAGCACCCC GGAAGGGGAG UGAAAGAGAU CCUGAA ACC GUGUGCCUAC AAGUAGUCAG AGCCCGUUAA CGGGUGAUGG CGUGCCUUUU GUAGAAUGAA CCGGCGAGUU ACGAUCU CG UGCAAGGUUA AGCAGAAGAU GCGGAGCCGC AGCGAAAGCG AGUCUGAAUA GGGCGCAUGA GUACGUGGUC GUAGACCC G AAACCAGGUG AUCUACCCAU GUCCAGGGUG AAGUUCAGGU AACACUGAAU GGAGGCCCGA ACCCACGCAC GUUGAAAAG UGCGGGGAUG AGGUGUGGGU AGGGGUGAAA UGCCAAUCGA ACCUGGAGAU AGCUGGUUCU CUCCGAAAUA GCUUUAGGGC UAGCCUCAA GGUAAGAGUC UUGGAGGUAG AGCACUGAUU GGACUAGGGG CCCCUACCGG GUUACCGAAU UCAGUCAAAC U CCGAAUGC CAAUGACUUA UCCUUGGGAG UCAGACUGCG AGUGAUAAGA UCCGUAGUCG AAAGGGAAAC AGCCCAGACC GC CAGCUAA GGUCCCAAAG UAUACGUUAA GUGGAAAAGG AUGUGGAGUU GCUUAGACAA CCAGGAUGUU GGCUUAGAAG CAG CCACCA UUUAAAGAGU GCGUAAUAGC UCACUGGUCG AGUGACUCUG CGCCGAAAAU GUACCGGGGC UAAACGUAUC ACCG AAGCU GCGGACUGUU CUUCGAACAG UGGUAGGAGA GCGUUCUAAG GGCUGUGAAG CCAGACCGGA AGGACUGGUG GAGCG CUUA GAAGUGAGAA UGCCGGUAUG AGUAGCGAAA GAGGGGUGAG AAUCCCCUCC ACCGAAUGCC UAAGGUUUCC UGAGGA AGG CUCGUCCGCU CAGGGUUAGU CGGGACCUAA GCCGAGGCCG AAAGGCGUAG GCGAUGGACA ACAGGUUGAU AUUCCUG UA CCACCUCCUC ACCAUUUGAG CAAUGGGGGG ACGCAGGAGG AUAGGGUAAG CGCGGUAUUG GAUAUCCGCG UCCAAGCA G UUAGGCUGGG AAAUAGGCAA AUCCGUUUCC CAUAAGGCUG AGCUGUGAUG GCGAGCGAAA UAUAGUAGCG AAGUUCCUG AUUCCACACU GCCAAGAAAA GCCUCUAGCG AGGUGAGAGG UGCCCGUACC GCAAACCGAC ACAGGUAGGC GAGGAGAGAA UCCUAAGGU GAUCGAGAGA ACUCUCGUUA AGGAACUCGG CAAAAUGACC CCGUAACUUC GGGAGAAGGG GUGCUCUGUU A GGGUGCAA GCCCGAGAGA GCCGCAGUGA AUAGGCCCAG GCGACUGUUU AGCAAAAACA CAGGUCUCUG CGAAGCCGUA AG GCGAAGU AUAGGGGCUG ACGCCUGCCC GGUGCUGGAA GGUUAAGAGG AGCGCUUAGC GUAAGCGAAG GUGCGAAUUG AAG CCCCAG UAAACGGCGG CCGUAACUAU AACGGUCCUA AGGUAGCGAA AUUCCUUGUC GGGUAAGUUC CGACCCGCAC GAAA GGCGC AACGAUCUGG GCACUGUCUC AACGAGAGAC UCGGUGAAAU UAUAGUACCU GUGAAGAUGC AGGUUACCCG CGACA GGAC GGAAAGACCC CGUGGAGCUU UACUGCAGCC UGAUAUUGAA UGUUGGUACA GCUUGUACAG GAUAGGUAGG AGCCUU GGA AACCGGAGCG CCAGCUUCGG UGGAGGCAUC GGUGGGAUAC UACCCUGGCU GUAUUGACCU UCUAACCCGC CGCCCUU AU CGGGCGGGGA GACAGUGUCA GGUGGGCAGU UUGACUGGGG CGGUCGCCUC CUAAAAGGUA ACGGAGGCGC CCAAAGGU U CCCUCAGAAU GGUUGGAAAU CAUUCGCAGA GUGUAAAGGC ACAAGGGAGC UUGACUGCGA GACCUACAAG UCGAGCAGG GACGAAAGUC GGGCUUAGUG AUCCGGUGGU UCCGCAUGGA AGGGCCAUCG CUCAACGGAU AAAAGCUACC CCGGGGAUAA CAGGCUUAU CUCCCCCAAG AGUCCACAUC GACGGGGAGG UUUGGCACCU CGAUGUCGGC UCAUCGCAUC CUGGGGCUGU A GUCGGUCC CAAGGGUUGG GCUGUUCGCC CAUUAAAGCG GUACGCGAGC UGGGUUCAGA ACGUCGUGAG ACAGUUCGGU CC CUAUCCG UCGCGGGCGC AGGAAAUUUG AGAGGAGCUG UCCUUAGUAC GAGAGGACCG GGAUGGACGC ACCGCUGGUG UAC CAGUUG UUCUGCCAAG GGCAUCGCUG GGUAGCUAUG UGCGGACGGG AUAAGUGCUG AAAGCAUCUA AGCAUGAAGC CCCC CUCAA GAUGAGAUUU CCCAUUCCGC AAGGAAGUAA GAUCCCUGAA AGAUGAUCAG GUUGAUAGGU CUGAGGUGGA AGUGU GGCG ACACAUGGAG CUGACAGAUA CUAAUCGAUC GAGGACUUAA CCAU

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Macromolecule #2: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 36.15752 KDa
SequenceString:
UGGUGGCGAU AGCGAAGAGG UCACACCCGU UCCCAUACCG AACACGGAAG UUAAGCUCUU CAGCGCCGAU GGUAGUCGGG GGUUUCCCC CUGUGAGAGU AGGACGCCGC CAA

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Macromolecule #31: mRNA

MacromoleculeName: mRNA / type: rna / ID: 31 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 872.556 Da
SequenceString:
CUU

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Macromolecule #33: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 33 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 503.369125 KDa
SequenceString: UUUAUCGGAG AGUUUGAUCC UGGCUCAGGA CGAACGCUGG CGGCGUGCCU AAUACAUGCA AGUCGAGCGG ACAGAUGGGA GCUUGCUCC CUGAUGUUAG CGGCGGACGG GUGAGUAACA CGUGGGUAAC CUGCCUGUAA GACUGGGAUA ACUCCGGGAA A CCGGGGCU ...String:
UUUAUCGGAG AGUUUGAUCC UGGCUCAGGA CGAACGCUGG CGGCGUGCCU AAUACAUGCA AGUCGAGCGG ACAGAUGGGA GCUUGCUCC CUGAUGUUAG CGGCGGACGG GUGAGUAACA CGUGGGUAAC CUGCCUGUAA GACUGGGAUA ACUCCGGGAA A CCGGGGCU AAUACCGGAU GGUUGUUUGA ACCGCAUGGU UCAAACAUAA AAGGUGGCUU CGGCUACCAC UUACAGAUGG AC CCGCGGC GCAUUAGCUA GUUGGUGAGG UAACGGCUCA CCAAGGCGAC GAUGCGUAGC CGACCUGAGA GGGUGAUCGG CCA CACUGG GACUGAGACA CGGCCCAGAC UCCUACGGGA GGCAGCAGUA GGGAAUCUUC CGCAAUGGAC GAAAGUCUGA CGGA GCAAC GCCGCGUGAG UGAUGAAGGU UUUCGGAUCG UAAAGCUCUG UUGUUAGGGA AGAACAAGUG CCGUUCGAAU AGGGC GGUA CCUUGACGGU ACCUAACCAG AAAGCCACGG CUAACUACGU GCCAGCAGCC GCGGUAAUAC GUAGGUGGCA AGCGUU GUC CGGAAUUAUU GGGCGUAAAG GGCUCGCAGG CGGUUUCUUA AGUCUGAUGU GAAAGCCCCC GGCUCAACCG GGGAGGG UC AUUGGAAACU GGGGAACUUG AGUGCAGAAG AGGAGAGUGG AAUUCCACGU GUAGCGGUGA AAUGCGUAGA GAUGUGGA G GAACACCAGU GGCGAAGGCG ACUCUCUGGU CUGUAACUGA CGCUGAGGAG CGAAAGCGUG GGGAGCGAAC AGGAUUAGA UACCCUGGUA GUCCACGCCG UAAACGAUGA GUGCUAAGUG UUAGGGGGUU UCCGCCCCUU AGUGCUGCAG CUAACGCAUU AAGCACUCC GCCUGGGGAG UACGGUCGCA AGACUGAAAC UCAAAGGAAU UGACGGGGGC CCGCACAAGC GGUGGAGCAU G UGGUUUAA UUCGAAGCAA CGCGAAGAAC CUUACCAGGU CUUGACAUCC UCUGACAAUC CUAGAGAUAG GACGUCCCCU UC GGGGGCA GAGUGACAGG UGGUGCAUGG UUGUCGUCAG CUCGUGUCGU GAGAUGUUGG GUUAAGUCCC GCAACGAGCG CAA CCCUUG AUCUUAGUUG CCAGCAUUCA GUUGGGCACU CUAAGGUGAC UGCCGGUGAC AAACCGGAGG AAGGUGGGGA UGAC GUCAA AUCAUCAUGC CCCUUAUGAC CUGGGCUACA CACGUGCUAC AAUGGACAGA ACAAAGGGCA GCGAAACCGC GAGGU UAAG CCAAUCCCAC AAAUCUGUUC UCAGUUCGGA UCGCAGUCUG CAACUCGACU GCGUGAAGCU GGAAUCGCUA GUAAUC GCG GAUCAGCAUG CCGCGGUGAA UACGUUCCCG GGCCUUGUAC ACACCGCCCG UCACACCACG AGAGUUUGUA ACACCCG AA GUCGGUGAGG UAACCUUUUA GGAGCCAGCC GCCGAAGGUG GGACAGAUGA UUGGGGUGAA GUCGUAACAA GGUAGCCG U AUCGGAAGGU GCGGCUGGAU CACCUCCUUU CUA

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Macromolecule #53: P-tRNA(Leu)

MacromoleculeName: P-tRNA(Leu) / type: rna / ID: 53 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.199311 KDa
SequenceString:
GCCGAGGUGG UGGAAUUGGU AGACACGCUA CCUUGAGGUG GUAGUUUACG GGUUCAAGUC CCGUCCUCGG UACCA

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Macromolecule #3: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 30.335125 KDa
SequenceString: MAIKKYKPTS NGRRGMTTSD FAEITTDKPE KSLLAPLHKK GGRNNQGKLT VRHQGGGHKR QYRVIDFKRD KDGIPGRVAT VEYDPNRSA NIALINYADG EKRYILAPKG IQVGTEIMSG PEADIKVGNA LPLINIPVGT VVHNIELKPG KGGQLVRSAG T SAQVLGKE ...String:
MAIKKYKPTS NGRRGMTTSD FAEITTDKPE KSLLAPLHKK GGRNNQGKLT VRHQGGGHKR QYRVIDFKRD KDGIPGRVAT VEYDPNRSA NIALINYADG EKRYILAPKG IQVGTEIMSG PEADIKVGNA LPLINIPVGT VVHNIELKPG KGGQLVRSAG T SAQVLGKE GKYVLVRLNS GEVRMILSAC RASIGQVGNE QHELINIGKA GRSRWKGIRP TVRGSVMNPN DHPHGGGEGR AP IGRKSPM SPWGKPTLGF KTRKKKNKSD KFIVRRRKNK

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Macromolecule #4: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 22.723348 KDa
SequenceString: MTKGILGRKI GMTQVFAENG DLIPVTVIEA APNVVLQKKT AENDGYEAIQ LGFDDKREKL SNKPEKGHVA KAETAPKRFV KELRGVEMD AYEVGQEVKV EIFSAGEIVD VTGVSKGKGF QGAIKRHGQS RGPMSHGSRY HRRPGSMGPV DPNRVFKGKL L PGRMGGEQ ...String:
MTKGILGRKI GMTQVFAENG DLIPVTVIEA APNVVLQKKT AENDGYEAIQ LGFDDKREKL SNKPEKGHVA KAETAPKRFV KELRGVEMD AYEVGQEVKV EIFSAGEIVD VTGVSKGKGF QGAIKRHGQS RGPMSHGSRY HRRPGSMGPV DPNRVFKGKL L PGRMGGEQ ITVQNLEIVK VDAERNLLLI KGNVPGAKKS LITVKSAVKS K

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Macromolecule #5: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 22.424951 KDa
SequenceString: MPKVALYNQN GSTAGDIELN ASVFGIEPNE SVVFDAILMQ RASLRQGTHK VKNRSEVRGG GRKPWRQKGT GRARQGSIRS PQWRGGGVV FGPTPRSYSY KLPKKVRRLA IKSVLSSKVI DNNIIVLEDL TLDTAKTKEM AAILKGLSVE KKALIVTADA N EAVALSAR ...String:
MPKVALYNQN GSTAGDIELN ASVFGIEPNE SVVFDAILMQ RASLRQGTHK VKNRSEVRGG GRKPWRQKGT GRARQGSIRS PQWRGGGVV FGPTPRSYSY KLPKKVRRLA IKSVLSSKVI DNNIIVLEDL TLDTAKTKEM AAILKGLSVE KKALIVTADA N EAVALSAR NIPGVTVVEA NGINVLDVVN HEKLLITKAA VEKVEEVLA

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Macromolecule #6: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 20.177564 KDa
SequenceString:
MNRLKEKYNK EIAPALMTKF NYDSVMQVPK IEKIVINMGV GDAVQNAKAI DSAVEELTFI AGQKPVVTRA KKSIAGFRLR EGMPIGAKV TLRGERMYDF LDKLISVSLP RVRDFRGVSK KSFDGRGNYT LGIKEQLIFP EIDYDKVTKV RGMDIVIVTT A NTDEEARE LLTQVGMPFQ K

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Macromolecule #7: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 19.543389 KDa
SequenceString:
MSRVGKKLLE IPSDVTVTLN DNNTVAVKGP KGELTRTFHP DMEIKVEDNV LTVARPSDQK EHRALHGTTR SLLGNMVEGV SKGFERGLE LVGVGYRASK SGNKLVLNVG YSHPVEIVPE EGIEIEVPSQ TKVVVKGTDK ERVGAIAANI RAVRSPEPYK G KGIRYEGE VVRRKEGKSA K

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Macromolecule #8: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 16.407104 KDa
SequenceString:
MRTTPMANAS TIERKWLVVD AAGKTLGRLS SEVAAILRGK HKPTYTPHVD TGDHVIIINA EKIELTGKKL TDKIYYRHTQ HPGGLKSRT ALEMRTNYPE KMLELAIKGM LPKGSLGRQM FKKLNVYRGS EHPHEAQKPE VYELRG

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Macromolecule #9: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 13.175288 KDa
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKQATPGG VVKKGEVVKA VIVRTKSGAR RSDGSYISFD ENACVIIRD DKSPRGTRIF GPVARELREN NFMKIVSLAP EVI

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Macromolecule #10: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 15.410694 KDa
SequenceString:
MKLHELKPSE GSRKTRNRVG RGIGSGNGKT AGKGHKGQNA RSGGGVRPGF EGGQMPLFQR LPKRGFTNIN RKEYAVVNLD KLNGFAEGT EVTPELLLET GVISKLNAGV KILGNGKLEK KLTVKANKFS ASAKEAVEAA GGTAEVI

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Macromolecule #11: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 16.223049 KDa
SequenceString:
MLLPKRVKYR REHRGKMRGR AKGGTEVHFG EFGIQALEAS WITNRQIEAA RIAMTRYMKR GGKVWIKIFP SKPYTAKPLE VRMGSGKGA PEGWVAVVKP GKVLFEISGV SEEVAREALR LASHKLPIKT KFVKREEIGG ESNES

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Macromolecule #12: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 13.774806 KDa
SequenceString:
MSYRKLGRTS AQRKAMLRDL TTDLIINERI ETTETRAKEL RSVVEKMITL GKRGDLHARR QAAAYIRNEV ANEENNQDAL QKLFSDIAT RYEERQGGYT RIMKLGPRRG DGAPMAIIEL V

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Macromolecule #13: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 12.993829 KDa
SequenceString:
MITKTSKNAA RLKRHARVRA KLSGTAERPR LNVFRSNKHI YAQIIDDVNG VTLASASTLD KDLNVESTGD TSAATKVGEL VAKRAAEKG ISDVVFDRGG YLYHGRVKAL ADAAREAGLK F

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Macromolecule #14: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 13.416853 KDa
SequenceString:
MQKLIEDITK EQLRTDLPAF RPGDTLRVHV KVVEGNRERI QIFEGVVIKR RGGGISETFT VRKISYGVGV ERTFPVHTPK IAKIEVVRY GKVRRAKLYY LRELRGKAAR IKEIRR

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Macromolecule #15: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 13.669189 KDa
SequenceString:
MPRVKGGTVT RKRRKKVLKL AKGYFGSKHT LYKVANQQVM KSGNYAFRDR RQKKRDFRKL WITRINAAAR MNGLSYSRLM HGLKLSGIE VNRKMLADLA VNDLTAFNQL ADAAKAQLNK

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Macromolecule #16: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 11.296081 KDa
SequenceString:
MYAIIKTGGK QIKVEEGQTV YIEKLAAEAG ETVTFEDVLF VGGDNVKVGN PTVEGATVTA KVEKQGRAKK ITVFRYKPKK NVHKKQGHR QPYTKVTIEK INA

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Macromolecule #17: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 12.481608 KDa
SequenceString:
MQAKAVARTV RIAPRKARLV MDLIRGKQVG EAVSILNLTP RAASPIIEKV LKSAIANAEH NYEMDANNLV ISQAFVDEGP TLKRFRPRA MGRASQINKR TSHITIVVSE KKEG

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Macromolecule #18: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 10.978813 KDa
SequenceString:
MKDPRDVLKR PVITERSADL MTEKKYTFEV DVRANKTEVK DAVESIFGVK VDKVNIMNYK GKSKRVGRYT GMTSRRRKAI VKLTADSKE IEIFEA

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Macromolecule #19: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 11.16612 KDa
SequenceString:
MHVKKGDKVM VISGKDKGKQ GTILAAFPKK DRVLVEGVNM VKKHSKPTQA NPQGGISNQE APIHVSNVMP LDPKTGEVTR VGYKVEDGK KVRVAKKSGQ VLDK

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Macromolecule #20: Nucleotide-binding protein ExpZ

MacromoleculeName: Nucleotide-binding protein ExpZ / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 60.272344 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MHHHHHHKEI VTLTNVSYEV KDQTVFKHVN ASVQQGDIIG IIGKNGAGKS TLLHLIHNDL APAQGQILRK DIKLALVEQE TAAYSFADQ TPAEKKLLEK WHVPLRDFHQ LSGGEKLKAR LAKGLSEDAD LLLLDQPTNH LDEKSLQFLI QQLKHYNGTV I LVSHDRYF ...String:
MHHHHHHKEI VTLTNVSYEV KDQTVFKHVN ASVQQGDIIG IIGKNGAGKS TLLHLIHNDL APAQGQILRK DIKLALVEQE TAAYSFADQ TPAEKKLLEK WHVPLRDFHQ LSGGEKLKAR LAKGLSEDAD LLLLDQPTNH LDEKSLQFLI QQLKHYNGTV I LVSHDRYF LDEAATKIWS LEDQTLIEFK GNYSGYMKFR EKKRLTQQRE YEKQQKMVER IEAQMNGLAS WSEKAHAQST KK EGFKEYH RVKAKRTDAQ IKSKQKRLEK ELEKAKAEPV TPEYTVRFSI DTTHKTGKRF LEVQNVTKAF GERTLFKNAN FTI QHGEKV AIIGPNGSGK TTLLNIILGQ ETAEGSVWVS PSANIGYLTQ EVFDLPLEQT PEELFENETF KARGHVQNLM RHLG FTAAQ WTEPIKHMSM GERVKIKLMA YILEEKDVLI LDQPTNHLDL PSREQLEETL SQYSGTLLAV SHDRYFLEKT TNSKL VISN NGIEKQLAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAA

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Macromolecule #21: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 10.391855 KDa
SequenceString:
MLRLDLQFFA SKKGVGSTKN GRDSEAKRLG AKRADGQFVT GGSILYRQRG TKIYPGENVG RGGDDTLFAK IDGTVKFERF GRDRKKVSV YPVAQ

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Macromolecule #22: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 6.826144 KDa
SequenceString:
MARKCVITGK KTTAGNNRSH AMNASKRTWG ANLQKVRILV NGKPKKVYVS ARALKSGKVE RV

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Macromolecule #23: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 7.728029 KDa
SequenceString:
MKANEIRDLT TAEIEQKVKS LKEELFNLRF QLATGQLENT ARIREVRKAI ARMKTVIRER EIAANK

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Macromolecule #24: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 6.650795 KDa
SequenceString:
MAKLEITLKR SVIGRPEDQR VTVRTLGLKK TNQTVVHEDN AAIRGMINKV SHLVSVKEQ

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Macromolecule #25: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 6.745073 KDa
SequenceString:
MAVPFRRTSK MKKRLRRTHF KLNVPGMTEC PSCGEMKLSH RVCKACGSYN GKDINVKSN

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Macromolecule #26: 50S ribosomal protein L33 1

MacromoleculeName: 50S ribosomal protein L33 1 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 5.915875 KDa
SequenceString:
MRVNITLACT ECGERNYISK KNKRNNPDRV EFKKYCPRDK KSTLHRETK

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Macromolecule #27: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 5.271332 KDa
SequenceString:
MKRTFQPNNR KRSKVHGFRS RMSSKNGRLV LARRRRKGRK VLSA

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Macromolecule #28: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 7.581128 KDa
SequenceString:
MPKMKTHRGS AKRFKKTGSG KLKRSHAYTS HLFANKSQKQ KRKLRKSAVV SAGDFKRIKQ MLANIK

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Macromolecule #29: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 4.318422 KDa
SequenceString:
MKVRPSVKPI CEKCKVIRRK GKVMVICENP KHKQKQG

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Macromolecule #30: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 7.458582 KDa
SequenceString:
MKAGIHPNFK KATVKCACGN EFETGSVKEE VRVEICSECH PFYTGRQKFA SADGRVDRFN KKYGLK

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Macromolecule #32: 50S ribosomal protein L1

MacromoleculeName: 50S ribosomal protein L1 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 25.026887 KDa
SequenceString: MAKKGKKYVE AAKLVDRSKA YDVSEAVALV KKTNTAKFDA TVEVAFRLGV DPRKNDQQIR GAVVLPNGTG KTQRVLVFAK GEKAKEAEA AGADFVGDTD YINKIQQGWF DFDVIVATPD MMGEVGKIGR VLGPKGLMPN PKTGTVTFEV EKAIGEIKAG K VEYRVDKA ...String:
MAKKGKKYVE AAKLVDRSKA YDVSEAVALV KKTNTAKFDA TVEVAFRLGV DPRKNDQQIR GAVVLPNGTG KTQRVLVFAK GEKAKEAEA AGADFVGDTD YINKIQQGWF DFDVIVATPD MMGEVGKIGR VLGPKGLMPN PKTGTVTFEV EKAIGEIKAG K VEYRVDKA GNIHVPIGKV SFEDEKLVEN FTTMYDTILK AKPAAAKGVY VKNVAVTSTM GPGVKVDSST FNVK

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Macromolecule #34: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 28.009297 KDa
SequenceString: MSVISMKQLL EAGVHFGHQT RRWNPKMKRY IFTERNGIYI IDLQKTVKKV EEAYNFTKNL AAEGGKILFV GTKKQAQDSV KEEAQRSGM YYVNQRWLGG TLTNFETIQK RIKRLKDIEK MQENGTFDVL PKKEVVQLKK ELERLEKFLG GIKDMKDLPD A LFIIDPRK ...String:
MSVISMKQLL EAGVHFGHQT RRWNPKMKRY IFTERNGIYI IDLQKTVKKV EEAYNFTKNL AAEGGKILFV GTKKQAQDSV KEEAQRSGM YYVNQRWLGG TLTNFETIQK RIKRLKDIEK MQENGTFDVL PKKEVVQLKK ELERLEKFLG GIKDMKDLPD A LFIIDPRK ERIAVAEARK LNIPIIGIVD TNCDPDEIDV VIPANDDAIR AVKLLTSKMA DAILEAKQGE EEAEVAEETA PE TETTTA

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Macromolecule #35: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 24.364887 KDa
SequenceString: MGQKVNPVGL RIGVIRDWES KWYAGKDYAD FLHEDLKIRE YISKRLSDAS VSKVEIERAA NRVNITIHTA KPGMVIGKGG SEVEALRKA LNSLTGKRVH INILEIKRAD LDAQLVADNI ARQLENRVSF RRAQKQQIQR TMRAGAQGVK TMVSGRLGGA D IARSEYYS ...String:
MGQKVNPVGL RIGVIRDWES KWYAGKDYAD FLHEDLKIRE YISKRLSDAS VSKVEIERAA NRVNITIHTA KPGMVIGKGG SEVEALRKA LNSLTGKRVH INILEIKRAD LDAQLVADNI ARQLENRVSF RRAQKQQIQR TMRAGAQGVK TMVSGRLGGA D IARSEYYS EGTVPLHTLR ADIDYATSEA DTTYGKLGVK VWIYRGEVLP TKKKNEEGGK

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Macromolecule #36: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 22.874271 KDa
SequenceString: MARYTGPSWK LSRRLGISLS GTGKELEKRP YAPGPHGPGQ RKKLSEYGLQ LQEKQKLRHM YGVNERQFRT LFDKAGKLAG KHGENFMIL LDSRLDNVVY KLGLARTRRQ ARQLVNHGHI LVDGSRVDIP SYLVKPGQTI GVREKSRNLS IIKESVEVNN F VPEYLTFD ...String:
MARYTGPSWK LSRRLGISLS GTGKELEKRP YAPGPHGPGQ RKKLSEYGLQ LQEKQKLRHM YGVNERQFRT LFDKAGKLAG KHGENFMIL LDSRLDNVVY KLGLARTRRQ ARQLVNHGHI LVDGSRVDIP SYLVKPGQTI GVREKSRNLS IIKESVEVNN F VPEYLTFD AEKLEGTFTR LPERSELAPE INEALIVEFY SR

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Macromolecule #37: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 17.650625 KDa
SequenceString:
MRRIDPSKLE LEERLVTVNR VAKVVKGGRR FRFAALVVVG DKNGHVGFGT GKAQEVPEAI RKAVEDAKKN LIEVPMVGTT IPHEIIGRF GAGNILLKPA SEGTGVIAGG PVRAVLELAG VADILSKSLG SNTPINMIRA TLQGLSELKR AEDVAKLRGK S VEELLG

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Macromolecule #38: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 11.140548 KDa
SequenceString:
MRKYEVMYII RPNIDEESKK AVIERFNNVL TSNGAEITGT KDWGKRRLAY EINDFRDGFY QIVNVQSDAA AVQEFDRLAK ISDDIIRHI VVKEEE

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Macromolecule #39: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 17.915879 KDa
SequenceString:
MPRKGPVAKR DVLPDPIYNS KLVSRLINKM MIDGKKGKSQ TILYKSFDII KERTGNDAME VFEQALKNIM PVLEVKARRV GGANYQVPV EVRPERRTTL GLRWLVNYAR LRGEKTMEER LANEILDAAN NTGAAVKKRE DTHKMAEANK AFAHYRW

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Macromolecule #40: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 14.901427 KDa
SequenceString:
MVMTDPIADM LTRIRNANMV RHEKLEIPAS KLKREIAEIL KREGFIRDVE FVEDSKQGII RVFLKYGQNN ERVITGLKRI SKPGLRVYA KSNEVPRVLN GLGIAIISTS QGVLTDKEAR AKQAGGEVLA YVW

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Macromolecule #41: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 14.335504 KDa
SequenceString:
MAQVQYYGTG RRKSSVARVR LVPGEGRIVV NNREISEHIP SAALIEDIKQ PLTLTETAGT YDVLVNVHGG GLSGQAGAIR HGIARALLE ADPEYRTTLK RAGLLTRDAR MKERKKYGLK GARRAPQFSK R

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Macromolecule #42: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 11.687661 KDa
SequenceString:
MAKQKIRIRL KAYDHRILDQ SAEKIVETAK RSGASVSGPI PLPTEKSVYT ILRAVHKYKD SREQFEMRTH KRLIDIVNPT PQTVDALMR LDLPSGVDIE IKL

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Macromolecule #43: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 13.952 KDa
SequenceString:
MAAARKSNTR KRRVKKNIES GIAHIRSTFN NTIVTITDTH GNAISWSSAG ALGFRGSRKS TPFAAQMAAE TAAKGSIEHG LKTLEVTVK GPGSGREAAI RALQAAGLEV TAIRDVTPVP HNGCRPPKRR RV

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Macromolecule #44: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 15.248736 KDa
SequenceString:
MPTINQLIRK GRVSKVENSK SPALNKGYNS FKKEHTNVSS PQKRGVCTRV GTMTPKKPNS ALRKYARVRL TNGIEVTAYI PGIGHNLQE HSVVLIRGGR VKDLPGVRYH IVRGALDTAG VENRAQGRSK YGTKKPKAK

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Macromolecule #45: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 13.818085 KDa
SequenceString:
MARIAGVDIP RDKRVVISLT YIFGIGRTTA QQVLKEAGVS EDTRVRDLTE EELGKIRDII DKLKVEGDLR REVSLNIKRL IEIGSYRGI RHRRGLPVRG QNSKNNARTR KGPRRTVANK KK

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Macromolecule #46: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 7.263803 KDa
SequenceString:
MAKKSMIAKQ QRTPKFKVQE YTRCERCGRP HSVIRKFKLC RICFRELAYK GQIPGVKKAS W

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Macromolecule #47: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 10.597224 KDa
SequenceString:
MAITQERKNQ LINEFKTHES DTGSPEVQIA ILTDSINNLN EHLRTHKKDH HSRRGLLKMV GKRRNLLTYL RNKDVTRYRE LINKLGLRR

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Macromolecule #48: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 10.153833 KDa
SequenceString:
MAVKIRLKRM GAKKSPFYRI VVADSRSPRD GRFIETVGTY NPVAKPAEVK IDEELALKWL QTGAKPSDTV RNLFSSQGIM EKFHNAKQG K

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Macromolecule #49: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 10.220979 KDa
SequenceString:
MSERNQRKVY QGRVVSDKMD KTITVVVETY KKHTLYGKRV KYSKKFKAHD ENNQAKIGDI VKIMETRPLS ATKRFRLVEV VEEAVII

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Macromolecule #50: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 8.990613 KDa
SequenceString:
MAGGRRGGRA KRRKVCYFTS NGITHIDYKD VDLLKKFVSE RGKILPRRVT GTNAKYQRKL TAAIKRARQM ALLPYVSGE

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Macromolecule #51: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 10.607309 KDa
SequenceString:
MARSLKKGPF VDGHLMTKIE KLNETDKKQV VKTWSRRSTI FPQFIGHTIA VYDGRKHVPV FISEDMVGHK LGEFAPTRTY KGHASDDKK TRR

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Macromolecule #52: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria)
Molecular weightTheoretical: 9.622217 KDa
SequenceString:
MPNIKSAIKR TKTNNERRVH NATIKSAMRT AIKQVEASVA NNEADKAKTA LTEAAKRIDK AVKTGLVHKN TAARYKSRLA KKVNGLSA

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Macromolecule #54: TELITHROMYCIN

MacromoleculeName: TELITHROMYCIN / type: ligand / ID: 54 / Number of copies: 1 / Formula: TEL
Molecular weightTheoretical: 812.004 Da
Chemical component information

ChemComp-TEL:
TELITHROMYCIN / antibiotic*YM / Telithromycin

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Macromolecule #55: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 55 / Number of copies: 2 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 1.425 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: Gctf (ver. 1.06)
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3j9w

Initial angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 2.1)
Final 3D classificationSoftware - Name: RELION (ver. 2.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1) / Number images used: 28972

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-6ha8:
Cryo-EM structure of the ABCF protein VmlR bound to the Bacillus subtilis ribosome

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