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- EMDB-3852: Elongation factor G-ribosome complex captures in the absence of i... -

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Basic information

Entry
Database: EMDB / ID: EMD-3852
TitleElongation factor G-ribosome complex captures in the absence of inhibitors.
Map dataElongation factor G-ribosome complex captured in the absence of inhibitors
Sample
  • Complex: EF-G bound to 70S ribosome
    • Complex: 70S ribosomeRibosome
      • RNA: x 3 types
      • Protein or peptide: x 53 types
    • Complex: tRNA PHE
      • RNA: x 1 types
    • Complex: Elongation factor GEF-G
      • Protein or peptide: x 1 types
    • Complex: mRNAMessenger RNA
      • RNA: x 1 types
  • Ligand: x 3 types
Function / homology
Function and homology information


translation elongation factor activity / large ribosomal subunit / regulation of translation / small ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome ...translation elongation factor activity / large ribosomal subunit / regulation of translation / small ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / GTPase activity / GTP binding / zinc ion binding / metal ion binding / cytoplasm
Similarity search - Function
: / Translation elongation factor EFG/EF2 / Elongation factor G, domain III / EFG, domain V / Elongation Factor G, domain II / Elongation Factor G, domain III / Ribosomal protein L1, bacterial-type / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV ...: / Translation elongation factor EFG/EF2 / Elongation factor G, domain III / EFG, domain V / Elongation Factor G, domain II / Elongation Factor G, domain III / Ribosomal protein L1, bacterial-type / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / 30S ribosomal protein Thx / 30S ribosomal protein Thx / Elongation factor G C-terminus / 30S ribosomal protein / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / EF-G domain III/V-like / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / Ribosomal protein S14, type Z / Ribosomal protein L1, conserved site / Ribosomal protein L1 / Ribosomal protein L1 signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Translation elongation factor EFTu-like, domain 2 / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Elongation factor Tu domain 2 / Ribosomal protein L1p/L10e family / Ribosomal protein L31 type A / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein S14/S29 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein S6, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L14P, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family
Similarity search - Domain/homology
Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL19 ...Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS12 / Elongation factor G / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bTHX / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL31 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein uL1 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL17
Similarity search - Component
Biological speciesThermus thermophilus HB8 (bacteria) / Escherichia coli (E. coli) / Thermus thermophilus (bacteria) / Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsMace K / Giudice E / Chat S / Gillet R
CitationJournal: Nucleic Acids Res / Year: 2018
Title: The structure of an elongation factor G-ribosome complex captured in the absence of inhibitors.
Authors: Kevin Macé / Emmanuel Giudice / Sophie Chat / Reynald Gillet /
Abstract: During translation's elongation cycle, elongation factor G (EF-G) promotes messenger and transfer RNA translocation through the ribosome. Until now, the structures reported for EF-G-ribosome ...During translation's elongation cycle, elongation factor G (EF-G) promotes messenger and transfer RNA translocation through the ribosome. Until now, the structures reported for EF-G-ribosome complexes have been obtained by trapping EF-G in the ribosome. These results were based on use of non-hydrolyzable guanosine 5'-triphosphate (GTP) analogs, specific inhibitors or a mutated EF-G form. Here, we present the first cryo-electron microscopy structure of EF-G bound to ribosome in the absence of an inhibitor. The structure reveals a natural conformation of EF-G·GDP in the ribosome, with a previously unseen conformation of its third domain. These data show how EF-G must affect translocation, and suggest the molecular mechanism by which fusidic acid antibiotic prevents the release of EF-G after GTP hydrolysis.
History
DepositionAug 21, 2017-
Header (metadata) releaseFeb 14, 2018-
Map releaseFeb 14, 2018-
UpdateApr 11, 2018-
Current statusApr 11, 2018Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5ot7
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_3852.png

Downloads & links

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Map

FileDownload / File: emd_3852.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationElongation factor G-ribosome complex captured in the absence of inhibitors
Voxel sizeX=Y=Z: 1.1025 Å
Density
Contour LevelBy AUTHOR: 0.04 / Movie #1: 0.05
Minimum - Maximum-0.27181298 - 0.45327133
Average (Standard dev.)0.0014783972 (±0.014340088)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 463.05 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.10251.10251.1025
M x/y/z420420420
origin x/y/z0.0000.0000.000
length x/y/z463.050463.050463.050
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS420420420
D min/max/mean-0.2720.4530.001

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Supplemental data

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Half map: unfiltered, unmasked half map 2

Fileemd_3852_half_map_1.map
Annotationunfiltered, unmasked half map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: unfiltered, unmasked half map 1

Fileemd_3852_half_map_2.map
Annotationunfiltered, unmasked half map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : EF-G bound to 70S ribosome

EntireName: EF-G bound to 70S ribosome
Components
  • Complex: EF-G bound to 70S ribosome
    • Complex: 70S ribosomeRibosome
      • RNA: 16S Ribosomal RNA
      • RNA: 23S Ribosomal RNA
      • RNA: 5S Ribosomal RNA
      • Protein or peptide: 50S ribosomal protein L25
      • Protein or peptide: 50S ribosomal protein L9
      • Protein or peptide: 30S ribosomal protein S2
      • Protein or peptide: 30S ribosomal protein S3
      • Protein or peptide: 30S ribosomal protein S4
      • Protein or peptide: 30S ribosomal protein S5
      • Protein or peptide: 30S ribosomal protein S6
      • Protein or peptide: 30S ribosomal protein S7
      • Protein or peptide: 30S ribosomal protein S8
      • Protein or peptide: 30S ribosomal protein S9
      • Protein or peptide: 30S ribosomal protein S10
      • Protein or peptide: 30S ribosomal protein S11
      • Protein or peptide: 30S ribosomal protein S12
      • Protein or peptide: 30S ribosomal protein S13
      • Protein or peptide: 30S ribosomal protein S14 type ZRibosome
      • Protein or peptide: 30S ribosomal protein S15
      • Protein or peptide: 30S ribosomal protein S16
      • Protein or peptide: 30S ribosomal protein S17
      • Protein or peptide: 30S ribosomal protein S18
      • Protein or peptide: 30S ribosomal protein S19
      • Protein or peptide: 30S ribosomal protein S20
      • Protein or peptide: 30S ribosomal protein ThxRibosome
      • Protein or peptide: 50S ribosomal protein L27
      • Protein or peptide: 50S ribosomal protein L28
      • Protein or peptide: 50S ribosomal protein L29
      • Protein or peptide: 50S ribosomal protein L30
      • Protein or peptide: 50S ribosomal protein L31
      • Protein or peptide: 50S ribosomal protein L32
      • Protein or peptide: 50S ribosomal protein L33
      • Protein or peptide: 50S ribosomal protein L34
      • Protein or peptide: 50S ribosomal protein L35
      • Protein or peptide: 50S ribosomal protein L36
      • Protein or peptide: 50S ribosomal protein L1
      • Protein or peptide: 50S ribosomal protein L2
      • Protein or peptide: 50S ribosomal protein L3
      • Protein or peptide: 50S ribosomal protein L4
      • Protein or peptide: 50S ribosomal protein L5
      • Protein or peptide: 50S ribosomal protein L6
      • Protein or peptide: Ribosomal protein uL10
      • Protein or peptide: Ribosomal protein uL11
      • Protein or peptide: Ribosomal protein bL12
      • Protein or peptide: 50S ribosomal protein L13
      • Protein or peptide: 50S ribosomal protein L14
      • Protein or peptide: 50S ribosomal protein L15
      • Protein or peptide: 50S ribosomal protein L16
      • Protein or peptide: 50S ribosomal protein L17
      • Protein or peptide: 50S ribosomal protein L18
      • Protein or peptide: 50S ribosomal protein L19
      • Protein or peptide: 50S ribosomal protein L20
      • Protein or peptide: 50S ribosomal protein L21
      • Protein or peptide: 50S ribosomal protein L22
      • Protein or peptide: 50S ribosomal protein L23
      • Protein or peptide: 50S ribosomal protein L24
    • Complex: tRNA PHE
      • RNA: tRNA-Phe
    • Complex: Elongation factor GEF-G
      • Protein or peptide: Elongation factor GEF-G
    • Complex: mRNAMessenger RNA
      • RNA: mRNAMessenger RNA
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: GUANOSINE-5'-DIPHOSPHATE

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Supramolecule #1: EF-G bound to 70S ribosome

SupramoleculeName: EF-G bound to 70S ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#59

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Supramolecule #2: 70S ribosome

SupramoleculeName: 70S ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1, #4-#27, #29-#59
Source (natural)Organism: Thermus thermophilus HB8 (bacteria)

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Supramolecule #3: tRNA PHE

SupramoleculeName: tRNA PHE / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #4: Elongation factor G

SupramoleculeName: Elongation factor G / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #28
Source (natural)Organism: Thermus thermophilus (bacteria)
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)

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Supramolecule #5: mRNA

SupramoleculeName: mRNA / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #3
Source (natural)Organism: Escherichia coli (E. coli)
Recombinant expressionOrganism: synthetic construct (others)

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Macromolecule #1: 16S Ribosomal RNA

MacromoleculeName: 16S Ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 489.331719 KDa
SequenceString: UGGAGAGUUU GAUCCUGGCU CAGGGUGAAC GCUGGCGGCG UGCCUAAGAC AUGCAAGUCG UGCGGGCCGC GGGGUUUUAC UCCGUGGUC AGCGGCGGAC GGGUGAGUAA CGCGUGGGUG ACCUACCCGG AAGAGGGGGA CAACCCGGGG AAACUCGGGC U AAUCCCCC ...String:
UGGAGAGUUU GAUCCUGGCU CAGGGUGAAC GCUGGCGGCG UGCCUAAGAC AUGCAAGUCG UGCGGGCCGC GGGGUUUUAC UCCGUGGUC AGCGGCGGAC GGGUGAGUAA CGCGUGGGUG ACCUACCCGG AAGAGGGGGA CAACCCGGGG AAACUCGGGC U AAUCCCCC AUGUGGACCC GCCCCUUGGG GUGUGUCCAA AGGGCUUUGC CCGCUUCCGG AUGGGCCCGC GUCCCAUCAG CU AGUUGGU GGGGUAAUGG CCCACCAAGG CGACGACGGG UAGCCGGUCU GAGAGGAUGG CCGGCCACAG GGGCACUGAG ACA CGGGCC CCACUCCUAC GGGAGGCAGC AGUUAGGAAU CUUCCGCAAU GGGCGCAAGC CUGACGGAGC GACGCCGCUU GGAG GAAGA AGCCCUUCGG GGUGUAAACU CCUGAACCCG GGACGAAACC CCCGACGAGG GGACUGACGG UACCGGGGUA AUAGC GCCG GCCAACUCCG UGCCAGCAGC CGCGGUAAUA CGGAGGGCGC GAGCGUUACC CGGAUUCACU GGGCGUAAAG GGCGUG UAG GCGGCCUGGG GCGUCCCAUG UGAAAGACCA CGGCUCAACC GUGGGGGAGC GUGGGAUACG CUCAGGCUAG ACGGUGG GA GAGGGUGGUG GAAUUCCCGG AGUAGCGGUG AAAUGCGCAG AUACCGGGAG GAACGCCGAU GGCGAAGGCA GCCACCUG G UCCACCCGUG ACGCUGAGGC GCGAAAGCGU GGGGAGCAAA CCGGAUUAGA UACCCGGGUA GUCCACGCCC UAAACGAUG CGCGCUAGGU CUCUGGGUCU CCUGGGGGCC GAAGCUAACG CGUUAAGCGC GCCGCCUGGG GAGUACGGCC GCAAGGCUGA AACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGUGGA GCAUGUGGUU UAAUUCGAAG CAACGCGAAG AACCUUACCA G GCCUUGAC AUGCUAGGGA ACCCGGGUGA AAGCCUGGGG UGCCCCGCGA GGGGAGCCCU AGCACAGGUG CUGCAUGGCC GU CGUCAGC UCGUGCCGUG AGGUGUUGGG UUAAGUCCCG CAACGAGCGC AACCCCCGCC GUUAGUUGCC AGCGGUUCGG CCG GGCACU CUAACGGGAC UGCCCGCGAA AGCGGGAGGA AGGAGGGGAC GACGUCUGGU CAGCAUGGCC CUUACGGCCU GGGC GACAC ACGUGCUACA AUGCCCACUA CAAAGCGAUG CCACCCGGCA ACGGGGAGCU AAUCGCAAAA AGGUGGGCCC AGUUC GGAU UGGGGUCUGC AACCCGACCC CAUGAAGCCG GAAUCGCUAG UAAUCGCGGA UCAGCCAUGC CGCGGUGAAU ACGUUC CCG GGCCUUGUAC ACACCGCCCG UCACGCCAUG GGAGCGGGCU CUACCCGAAG UCGCCGGGAG CCUACGGGCA GGCGCCG AG GGUAGGGCCC GUGACUGGGG CGAAGUCGUA ACAAGGUAGC UGUACCGGAA GGUGCGGCUG GAUCA

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Macromolecule #2: tRNA-Phe

MacromoleculeName: tRNA-Phe / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.485539 KDa
SequenceString:
GCCCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUGAAAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA

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Macromolecule #3: mRNA

MacromoleculeName: mRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 4.88993 KDa
SequenceString:
AGGAGGUGAG GUUUU

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Macromolecule #4: 23S Ribosomal RNA

MacromoleculeName: 23S Ribosomal RNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 943.51575 KDa
SequenceString: AGAUGGUAAG GGCCCACGGU GGAUGCCUCG GCACCCGAGC CGAUGAAGGA CGUGGCUACC UGCGAUAAGC CAGGGGGAGC CGGUAGCGG GCGUGGAUCC CUGGAUGUCC GAAUGGGGGA ACCCGGCCGG CGGGAACGCC GGUCACCGCG CUUUUGCGCG G GGGGAACC ...String:
AGAUGGUAAG GGCCCACGGU GGAUGCCUCG GCACCCGAGC CGAUGAAGGA CGUGGCUACC UGCGAUAAGC CAGGGGGAGC CGGUAGCGG GCGUGGAUCC CUGGAUGUCC GAAUGGGGGA ACCCGGCCGG CGGGAACGCC GGUCACCGCG CUUUUGCGCG G GGGGAACC UGGGGAACUG AAACAUCUCA GUACCCAGAG GAGAGGAAAG AGAAAUCGAC UCCCUGAGUA GCGGCGAGCG AA AGGGGAC CAGCCUAAAC CGUCCGGCUU GUCCGGGCGG GGUCGUGGGG CCCUCGGACA CCGAAUCCCC AGCCUAGCCG AAG CUGUUG GGAAGCAGCG CCAGAGAGGG UGAAAGCCCC GUAGGCGAAA GGUGGGGGGA UAGGUGAGGG UACCCGAGUA CCCC GUGGU UCGUGGAGCC AUGGGGGAAU CUGGGCGGAC CACCGCCUAA GGCUAAGUAC UCCGGGUGAC CGAUAGCGCA CCAGU ACCG UGAGGGAAAG GUGAAAAGAA CCCCGGGAGG GGAGUGAAAU AGAGCCUGAA ACCGUGGGCU UACAAGCAGU CACGGC CCC GCAAGGGGUU GUGGCGUGCC UAUUGAAGCA UGAGCCGGCG ACUCACGGUC GUGGGCGAGC UUAAGCCGUU GAGGCGG AG GCGUAGGGAA ACCGAGUCCG AACAGGGCGC AAGCGGGCCG CACGCGGCCC GCAAAGUCCG CGGCCGUGGA CCCGAAAC C GGGCGAGCUA GCCCUGGCCA GGGUGAAGCU GGGGUGAGAC CCAGUGGAGG CCCGAACCGG UGGGGGAUGC AAACCCCUC GGAUGAGCUG GGGCUAGGAG UGAAAAGCUA ACCGAGCCCG GAGAUAGCUG GUUCUCCCCG AAAUGACUUU AGGGUCAGCC UCAGGCGCU GACUGGGGCC UGUAGAGCAC UGAUAGGGCU AGGGGGCCCA CCAGCCUACC AAACCCUGUC AAACUCCGAA G GGUCCCAG GUGGAGCCUG GGAGUGAGGG CGCGAGCGAU AACGUCCGCG UCCGAGCGCG GGAACAACCG AGACCGCCAG CU AAGGCCC CCAAGUCUGG GCUAAGUGGU AAAGGAUGUG GCGCGGCGAA GACAGCCAGG AGGUUGGCUU AGAAGCAGCC AUC CUUUAA AGAGUGCGUA AUAGCUCACU GGUCGAGUGG CGCCGCGCCG AAAAUGAUCG GGGCUUAAGC CCAGCGCCGA AGCU GCGGG UCUGGGGGAU GACCCCAGGC GGUAGGGGAG CGUUCCCGAU GCCGAUGAAG GCCGACCCGC GAGGGCGGCU GGAGG UAAG GGAAGUGCGA AUGCCGGCAU GAGUAACGAU AAAGAGGGUG AGAAUCCCUC UCGCCGUAAG CCCAAGGGUU CCUACG CAA UGGUCGUCAG CGUAGGGUUA GGCGGGACCU AAGGUGAAGC CGAAAGGCGU AGCCGAAGGG CAGCCGGUUA AUAUUCC GG CCCUUCCCGC AGGUGCGAUG GGGGGACGCU CUAGGCUAGG GGGACCGGAG CCAUGGACGA GCCCGGCCAG AAGCGCAG G GUGGGAGGUA GGCAAAUCCG CCUCCCAACA AGCUCUGCGU GGUGGGGAAG CCCGUACGGG UGACAACCCC CCGAAGCCA GGGAGCCAAG AAAAGCCUCU AAGCACAACC UGCGGGAACC CGUACCGCAA ACCGACACAG GUGGGCGGGU GCAAGAGCAC UCAGGCGCG CGGGAGAACC CUCGCCAAGG AACUCUGCAA GUUGGCCCCG UAACUUCGGG AGAAGGGGUG CUCCCUGGGG U GAUGAGCC CCGGGGAGCC GCAGUGAACA GGCUCUGGCG ACUGUUUACC AAAAACACAG CUCUCUGCGA ACUCGUAAGA GG AGGUAUA GGGAGCGACG CUUGCCCGGU GCCGGAAGGU CAAGGGGAGG GGUGCAAGCC CCGAACCGAA GCCCCGGUGA ACG GCGGCC GUAACUAUAA CGGUCCUAAG GUAGCGAAAU UCCUUGUCGG GUAAGUUCCG ACCUGCACGA AAAGCGUAAC GACC GGAGC GCUGUCUCGG CGAGGGACCC GGUGAAAUUG AACUGGCCGU GAAGAUGCGG CCUACCCGUG GCAGGACGAA AAGAC CCCG UGGAGCUUUA CUGCAGCCUG GUGUUGGCUC UUGGUCGCGC CUGCGUAGGA UAGGUGGGAG CCUGUAAACC CCCGCC UCC GGGUGGGGGG GAGGCGCCGG UGAAAUACCA CCCUGGCGCG GCUGGGGGCC UAACCCUCGG AUGGGGGGAC AGCGCUU GG CGGGCAGUUU GACUGGGGCG GUCGCCUCCU AAAAGGUAAC GGAGGCGCCC AAAGGUCCCC UCAGGCGGGA CGGAAAUC C GCCGGAGAGC GCAAGGGUAG AAGGGGGCCU GACUGCGAGG CCUGCAAGCC GAGCAGGGGC GAAAGCCGGG CCUAGUGAA CCGGUGGUCC CGUGUGGAAG GGCCAUCGAU CAACGGAUAA AAGUUACCCC GGGGAUAACA GGCUGAUCUC CCCCGAGCGU CCACAGCGG CGGGGAGGUU UGGCACCUCG AUGUCGGCUC GUCGCAUCCU GGGGCUGAAG AAGGUCCCAA GGGUUGGGCU G UUCGCCCA UUAAAGCGGC ACGCGAGCUG GGUUCAGAAC GUCGUGAGAC AGUUCGGUCU CUAUCCGCCA CGGGCGCAGG AG GCUUGAG GGGGGCUCUU CCUAGUACGA GAGGACCGGA AGGGACGCAC CUCUGGUUUC CCAGCUGUCC CUCCAGGGGC AUA AGCUGG GUAGCCAUGU GCGGAAGGGA UAACCGCUGA AAGCAUCUAA GCGGGAAGCC CGCCCCAAGA UGAGGCCUCC CACG GCGUC AAGCCGGUAA GGACCCGGGA AGACCACCCG GUGGAUGGGC CGGGGGUGUA AGCGCCGCGA GGCGUUGAGC CGACC GGUC CCAAUCGUCC GAGGUCU

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Macromolecule #5: 5S Ribosomal RNA

MacromoleculeName: 5S Ribosomal RNA / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 38.553 KDa
SequenceString:
UCCCCCGUGC CCAUAGCGGC GUGGAACCAC CCGUUCCCAU UCCGAACACG GAAGUGAAAC GCGCCAGCGC CGAUGGUACU GGGCGGGCG ACCGCCUGGG AGAGUAGGUC GGUGCGGGGG

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Macromolecule #6: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 20.10715 KDa
SequenceString:
YRLKAYYREG EKPSALRRAG KLPGVMYNRH LNRKVYVDLV EFDKVFRQAS IHHVIVLELP DGQSLPTLVR QVNLDKRRRR PEHVDFFVL SDEPVEMYVP LRFVGTPAGV RAGGVLQEIH RDILVKVSPR NIPEFIEVDV SGLEIGDSLH ASDLKLPPGV E LAVSPEET IAAVVPPEDV

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Macromolecule #7: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 16.164937 KDa
SequenceString:
MKVILLEPLE NLGDVGQVVD VKPGYARNYL LPRGLAVLAT ESNLKALEAR IRAQAKRLAE RKAEAERLKE ILENLTLTIP VRAGETKIY GSVTAKDIAE ALSRQHGVTI DPKRLALEKP IKELGEYVLT YKPHPEVPIQ LKVSVVA

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Macromolecule #8: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 27.116385 KDa
SequenceString: VKELLEAGVH FGHERKRWNP KFARYIYAER NGIHIIDLQK TMEELERTFR FIEDLAMRGG TILFVGTKKQ AQDIVRMEAE RAGMPYVNQ RWLGGMLTNF KTISQRVHRL EELEALFASP EIEERPKKEQ VRLKHELERL QKYLSGFRLL KRLPDAIFVV D PTKEAIAV ...String:
VKELLEAGVH FGHERKRWNP KFARYIYAER NGIHIIDLQK TMEELERTFR FIEDLAMRGG TILFVGTKKQ AQDIVRMEAE RAGMPYVNQ RWLGGMLTNF KTISQRVHRL EELEALFASP EIEERPKKEQ VRLKHELERL QKYLSGFRLL KRLPDAIFVV D PTKEAIAV REARKLFIPV IALADTDSDP DLVDYIIPGN DDAIRSIQLI LSRAVDLIIQ ARGGVVEPSP SYALVQE

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Macromolecule #9: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 22.975588 KDa
SequenceString: GNKIHPIGFR LGITRDWESR WYAGKKQYRH LLLEDQRIRG LLEKELYSAG LARVDIERAA DNVAVTVHVA KPGVVIGRGG ERIRVLREE LAKLTGKNVA LNVQEVQNPN LSAPLVAQRV AEQIERRFAV RRAIKQAVQR VMESGAKGAK VIVSGRIGGA E QARTEWAA ...String:
GNKIHPIGFR LGITRDWESR WYAGKKQYRH LLLEDQRIRG LLEKELYSAG LARVDIERAA DNVAVTVHVA KPGVVIGRGG ERIRVLREE LAKLTGKNVA LNVQEVQNPN LSAPLVAQRV AEQIERRFAV RRAIKQAVQR VMESGAKGAK VIVSGRIGGA E QARTEWAA QGRVPLHTLR ANIDYGFALA RTTYGVLGVK AYIFLGEVI

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Macromolecule #10: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 24.242254 KDa
SequenceString: GRYIGPVCRL CRREGVKLYL KGERCYSPKC AMERRPYPPG QHGQKRARRP SDYAVRLREK QKLRRIYGIS ERQFRNLFEE ASKKKGVTG SVFLGLLESR LDNVVYRLGF AVSRRQARQL VRHGHITVNG RRVDLPSYRV RPGDEIAVAE KSRNLELIRQ N LEAMKGRK ...String:
GRYIGPVCRL CRREGVKLYL KGERCYSPKC AMERRPYPPG QHGQKRARRP SDYAVRLREK QKLRRIYGIS ERQFRNLFEE ASKKKGVTG SVFLGLLESR LDNVVYRLGF AVSRRQARQL VRHGHITVNG RRVDLPSYRV RPGDEIAVAE KSRNLELIRQ N LEAMKGRK VGPWLSLDVE GMKGKFLRLP DREDLALPVN EQLVIEFYSR

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Macromolecule #11: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 16.460193 KDa
SequenceString:
DFEEKMILIR RTARMQAGGR RFRFGALVVV GDRQGRVGLG FGKAPEVPLA VQKAGYYARR NMVEVPLQNG TIPHEIEVEF GASKIVLKP AAPGTGVIAG AVPRAILELA GVTDILTKEL GSRNPINIAY ATMEALRQLR TKADVERLRK GE

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Macromolecule #12: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.988753 KDa
SequenceString:
MRRYEVNIVL NPNLDQSQLA LEKEIIQRAL ENYGARVEKV EELGLRRLAY PIAKDPQGYF LWYQVEMPED RVNDLARELR IRDNVRRVM VVKSQEPFLA NA

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Macromolecule #13: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 17.919775 KDa
SequenceString:
ARRRRAEVRQ LQPDLVYGDV LVTAFINKIM RDGKKNLAAR IFYDACKIIQ EKTGQEPLKV FKQAVENVKP RMEVRSRRVG GANYQVPME VSPRRQQSLA LRWLVQAANQ RPERRAAVRI AHELMDAAEG KGGAVKKKED VERMAEANRA YAHYRW

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Macromolecule #14: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 15.86857 KDa
SequenceString:
MLTDPIADML TRIRNATRVY KESTDVPASR FKEEILRILA REGFIKGYER VDVDGKPYLR VYLKYGPRRQ GPDPRPEQVI HHIRRISKP GRRVYVGVKE IPRVRRGLGI AILSTSKGVL TDREARKLGV GGELICEVW

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Macromolecule #15: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 14.279419 KDa
SequenceString:
EQYYGTGRRK EAVARVFLRP GNGKVTVNGQ DFNEYFQGLV RAVAALEPLR AVDALGHFDA YITVRGGGKS GQIDAIKLGI ARALVQYNP DYRAKLKPLG FLTRDARVVE RKKYGKHKAR RAPQYSKR

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Macromolecule #16: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.398308 KDa
SequenceString:
KIRIKLRGFD HKTLDASAQK IVEAARRSGA QVSGPIPLPT RVRRFTVIRG PFKHKDSREH FELRTHNRLV DIINPNRKTI EQLMTLDLP TGVEIEIKTV

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Macromolecule #17: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 12.606369 KDa
SequenceString:
KRQVASGRAY IHASYNNTIV TITDPDGNPI TWSSGGVIGY KGSRKGTPYA AQLAALDAAK KAMAYGMQSV DVIVRGTGAG REQAIRALQ ASGLQVKSIV DDTPVPHNGC RPKKKFRKAS

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Macromolecule #18: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 13.875388 KDa
SequenceString:
PTINQLVRKG REKVRKKSKV PALKGAPFRR GVCTVVRTVT PKKPNSALRK VAKVRLTSGY EVTAYIPGEG HNLQEHSVVL IRGGRVKDL PGVRYHIVRG VYDAAGVKDR KKSRSKYGTK KPKEAA

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Macromolecule #19: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 13.494739 KDa
SequenceString:
ARIAGVEIPR NKRVDVALTY IYGIGKARAK EALEKTGINP ATRVKDLTEA EVVRLREYVE NTWKLEGELR AEVAANIKRL MDIGCYRGL RHRRGLPVRG QRTRTNARTR KGPRKTVAGK

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Macromolecule #20: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 7.027529 KDa
SequenceString:
ARKALIEKAK RTPKFKVRAY TRCVRCGRAR SVYRFFGLCR ICLRELAHKG QLPGVRKASW

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Macromolecule #21: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 10.447213 KDa
SequenceString:
PITKEEKQKV IQEFARFPGD TGSTEVQVAL LTLRINRLSE HLKVHKKDHH SHRGLLMMVG QRRRLLRYLQ REDPERYRAL IEKLGIRG

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Macromolecule #22: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 9.995546 KDa
SequenceString:
MVKIRLARFG SKHNPHYRIV VTDARRKRDG KYIEKIGYYD PRKTTPDWLK VDVERARYWL SVGAQPTDTA RRLLRQAGVF RQEA

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Macromolecule #23: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.880098 KDa
SequenceString:
PKKVLTGVVV SDKMQKTVTV LVERQFPHPL YGKVIKRSKK YLAHDPEEKY KLGDVVEIIE SRPISKRKRF RVLRLVESGR MDLVEKYLI RRQNYESLSK R

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Macromolecule #24: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 8.155812 KDa
SequenceString:
KAKVKATLGE FDLRDYRNVE VLKRFLSETG KILPRRRTGL SAKEQRILAK TIKRARILGL LPFTEKLVRK

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Macromolecule #25: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 9.961599 KDa
SequenceString:
SLKKGVFVDD HLLEKVLELN AKGEKRLIKT WSRRSTIVPE MVGHTIAVYN GKQHVPVYIT ENMVGHKLGE FAPTRTYRGH GKEAKATK

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Macromolecule #26: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 10.921086 KDa
SequenceString:
RNLSALKRHR QSLKRRLRNK AKKSAIKTLS KKAIQLAQEG KAEEALKIMR KAESLIDKAA KGSTLHKNAA ARRKSRLMRK VRQLLEAAG APLIGGGLSA

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Macromolecule #27: 30S ribosomal protein Thx

MacromoleculeName: 30S ribosomal protein Thx / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 3.089655 KDa
SequenceString:
GKGDRRTRRG KIWRGTYGKY RPRKK

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Macromolecule #28: Elongation factor G

MacromoleculeName: Elongation factor G / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579
Molecular weightTheoretical: 76.54757 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: KVEYDLKRLR NIGIAAHIDA GKTTTTERIL YYTGRIHKIG EVHEGAATMD FMEQERERGI TITAAVTTCF WKDHRINIID TPGHVDFTI EVERSMRVLD GAIVVFDSSQ GVEPQSETVW RQAEKYKVPR IAFANKMDKT GADLWLVIRT MQERLGARPV V MQLPIGRE ...String:
KVEYDLKRLR NIGIAAHIDA GKTTTTERIL YYTGRIHKIG EVHEGAATMD FMEQERERGI TITAAVTTCF WKDHRINIID TPGHVDFTI EVERSMRVLD GAIVVFDSSQ GVEPQSETVW RQAEKYKVPR IAFANKMDKT GADLWLVIRT MQERLGARPV V MQLPIGRE DTFSGIIDVL RMKAYTYGND LGTDIREIPI PEEYLDQARE YHEKLVEVAA DFDENIMLKY LEGEEPTEEE LV AAIRKGT IDLKITPVFL GSALKNKGVQ LLLDAVVDYL PSPLDIPPIK GTTPEGEVVE IHPDPNGPLA ALAFKIMADP YVG RLTFIR VYSGTLTSGS YVYNTTKGRK ERVARLLRMH ANHREEVEEL KAGDLGAVVG LKETITGDTL VGEDAPRVIL ESIE VPEPV IDVAIEPKTK ADQEKLSQAL ARLAEEDPTF RVSTHPETGQ TIISGMGELH LEIIVDRLKR EFKVDANVGK PQVAY RETI TKPVDVEGKF IRQTGGRGQY GHVKIKVEPL PRGSGFEFVN AIVGGVIPKE YIPAVQKGIE EAMQSGPLIG FPVVDI KVT LYDGSYHEVD SSEMAFKIAG SMAIKEAVQK GDPVILEPIM RVEVTTPEEY MGDVIGDLNA RRGQILGMEP RGNAQVI RA FVPLAEMFGY ATDLRSKTQG RGSFVMFFDH YQEVPKQVQE KLIKG

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Macromolecule #29: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 8.760084 KDa
SequenceString:
GSTRNGRDSQ AKRLGVKRYE GQVVRAGNIL VRQRGTRFKP GKNVGMGRDF TLFALVDGVV EFQDRGRLGR YVHVRPLA

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Macromolecule #30: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 10.559571 KDa
SequenceString:
KVCEISGKRP IVANSIQRRG KAKREGGVGK KTTGISKRRQ YPNLQKVRVR VAGQEITFRV AASHIPKVYE LVERAKGLKL EGLSPKEIK KELLK

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Macromolecule #31: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 8.539062 KDa
SequenceString:
KLSEVRKQLE EARKLSPVEL EKLVREKKRE LMELRFQASI GQLSQNHKIR DLKRQIARLL TVLNEKRRQN A

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Macromolecule #32: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 6.799126 KDa
SequenceString:
MPRLKVKLVK SPIGYPKDQK AALKALGLRR LQQERVLEDT PAIRGNVEKV AHLVRVEVVE

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Macromolecule #33: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 8.300543 KDa
SequenceString:
MKEGIHPKLV PARIICGCGN VIETYSTKPE IYVEVCSKCH PFYTGQQRFV DTEGRVERFQ RRYGDSYRKG R

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Macromolecule #34: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 6.590854 KDa
SequenceString:
AKHPVPKKKT SKARRDARRS HHALTPPTLV PCPECKAMKP PHTVCPECGY YAGRKVLEV

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Macromolecule #35: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 6.214431 KDa
SequenceString:
VRIKLLLECT ECKRRNYATE KNKRNTPNKL ELRKYCPWCR KHTVHREVKI

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Macromolecule #36: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 5.975255 KDa
SequenceString:
MKRTWQPNRR KRAKTHGFRA RMRTPGGRKV LKRRRQKGRW RLTPAVRK

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Macromolecule #37: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 7.374981 KDa
SequenceString:
PKMKTHKGAK KRVKITASGK VVAMKTGKRH LNWQKSGKEI RQKGRKFVLA KPEAERIKLL LPYE

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Macromolecule #38: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 4.435411 KDa
SequenceString:
MKVRASVKRI CDKCKVIRRH GRVYVICENP KHKQRQG

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Macromolecule #39: 50S ribosomal protein L1

MacromoleculeName: 50S ribosomal protein L1 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 24.644408 KDa
SequenceString: KHGKRYRALL EKVDPNKVYT IDEAARLVKE LATAKFDETV EVHAKLGIDP RRSDQNVRGT VSLPHGLGKQ VRVLAIAKGE KIKEAEEAG ADYVGGEEII QKILDGWMDF DAVVATPDVM GAVGSKLGRI LGPRGLLPNP KAGTVGFNIG EIIREIKAGR I EFRNDKTG ...String:
KHGKRYRALL EKVDPNKVYT IDEAARLVKE LATAKFDETV EVHAKLGIDP RRSDQNVRGT VSLPHGLGKQ VRVLAIAKGE KIKEAEEAG ADYVGGEEII QKILDGWMDF DAVVATPDVM GAVGSKLGRI LGPRGLLPNP KAGTVGFNIG EIIREIKAGR I EFRNDKTG AIHAPVGKAS FPPEKLADNI RAFIRALEAH KPEGAKGTFL RSVYVTTTMG PSVRINPHS

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Macromolecule #40: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 30.401355 KDa
SequenceString: AVKKFKPYTP SRRFMTVADF SEITKTEPEK SLVKPLKKTG GRNNQGRITV RFRGGGHKRL YRIIDFKRWD KVGIPAKVAA IEYDPNRSA RIALLHYVDG EKRYIIAPDG LQVGQQVVAG PDAPIQVGNA LPLRFIPVGT VVHAVELEPK KGAKLARAAG T SAQIQGRE ...String:
AVKKFKPYTP SRRFMTVADF SEITKTEPEK SLVKPLKKTG GRNNQGRITV RFRGGGHKRL YRIIDFKRWD KVGIPAKVAA IEYDPNRSA RIALLHYVDG EKRYIIAPDG LQVGQQVVAG PDAPIQVGNA LPLRFIPVGT VVHAVELEPK KGAKLARAAG T SAQIQGRE GDYVILRLPS GELRKVHGEC YATVGAVGNA DHKNIVLGKA GRSRWLGRRP HVRGAAMNPV DHPHGGGEGR AP RGRPPAS PWGWQTKGLK TRKRRKPSSR FIIARRKK

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Macromolecule #41: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 22.321031 KDa
SequenceString: MKGILGVKVG MTRIFRDDRA VPVTVILAGP CPVVQRRTPE KDGYTAVQLG FLPQNPKRVN RPLKGHFAKA GVEPVRILRE IRDFNPEGD TVTVEIFKPG ERVDVTGTSK GRGFAGVMKR WNFAGGPDSH GAHKIHRHPG SIGNRKTPGR VYKGKKMAGH Y GAERVTVM ...String:
MKGILGVKVG MTRIFRDDRA VPVTVILAGP CPVVQRRTPE KDGYTAVQLG FLPQNPKRVN RPLKGHFAKA GVEPVRILRE IRDFNPEGD TVTVEIFKPG ERVDVTGTSK GRGFAGVMKR WNFAGGPDSH GAHKIHRHPG SIGNRKTPGR VYKGKKMAGH Y GAERVTVM NLEVVDVIPE ENLLLVKGAV PGPNGGLVIV RETKKAA

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Macromolecule #42: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 23.071721 KDa
SequenceString: MKEVAVYQIP VLSPSGRREL AADLPAEINP HLLWEVVRWQ LAKRRRGTAS TKTRGEVAYS GRKIWPQKHT GRARHGDIGA PIFVGGGVV FGPKPRDYSY TLPKKVRKKG LAMAVADRAR EGKLLLVEAF AGVNGKTKEF LAWAKEAGLD GSESVLLVTG N ELVRRAAR ...String:
MKEVAVYQIP VLSPSGRREL AADLPAEINP HLLWEVVRWQ LAKRRRGTAS TKTRGEVAYS GRKIWPQKHT GRARHGDIGA PIFVGGGVV FGPKPRDYSY TLPKKVRKKG LAMAVADRAR EGKLLLVEAF AGVNGKTKEF LAWAKEAGLD GSESVLLVTG N ELVRRAAR NLPWVVTLAP EGLNVYDIVR TERLVMDLDA WEVFQNRIGG

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Macromolecule #43: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 20.720127 KDa
SequenceString:
DVALKRKYYE EVRPELIRRF GYQNVWEVPR LEKVVINQGL GEAKEDARIL EKAAQELALI TGQKPAVTRA KKSISNFKLR KGMPIGLRV TLRRDRMWIF LEKLLNVALP RIRDFRGLNP NSFDGRGNYN LGLREQLIFP EITYDMVDAL RGMDIAVVTT A ETDEEARA LLELLGFPFR K

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Macromolecule #44: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 19.182436 KDa
SequenceString:
MSRIGRLPIP VPKGVSVEVA PGRVKVKGPK GELEVPVSPE MRVVVEEGVV RVERPSDERR HKSLHGLTRT LIANAVKGVS EGYSKELLI KGIGYRARLV GRALELTVGF SHPVVVEPPE GITFEVPEPT RVRVSGIDKQ KVGQVAANIR AIRKPSAYHE K GIYYAGEP VRLKPGKA

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Macromolecule #45: Ribosomal protein uL10

MacromoleculeName: Ribosomal protein uL10 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.109704 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)L(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

+
Macromolecule #46: Ribosomal protein uL11

MacromoleculeName: Ribosomal protein uL11 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.932701 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #47: Ribosomal protein bL12

MacromoleculeName: Ribosomal protein bL12 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 6.060462 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #48: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 15.828771 KDa
SequenceString:
MKTYVPKQVE PRWVLIDAEG KTLGRLATKI ATLLRGKHRP DWTPNVAMGD FVVVVNADKI RVTGKKLEQK IYTRYSGYPG GLKKIPLEK MLATHPERVL EHAVKGMLPK GPLGRRLFKR LKVYAGPDHP HQAQRPEKLE

+
Macromolecule #49: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 13.323612 KDa
SequenceString:
MIQPQTYLEV ADNTGARKIM CIRVLKGSNA KYATVGDVIV ASVKEAIPRG AVKEGDVVKA VVVRTKKEIK RPDGSAIRFD DNAAVIINN QLEPRGTRVF GPVARELREK GFMKIVSLAP EVL

+
Macromolecule #50: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 16.319142 KDa
SequenceString:
MKLSDLRPNP GANKRRKRVG RGPGSGHGKT ATRGHKGQKS RSGGLKDPRR FEGGRSTTLM RLPKRGMQGQ VPGEIKRPRY QGVNLKDLA RFEGEVTPEL LVRAGLLKKG YRLKILGEGE AKPLKVVAHA FSKSALEKLK AAGGEPVLLE A

+
Macromolecule #51: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 15.993909 KDa
SequenceString:
MLMPRRMKYR KQQRGRLKGA TKGGDYVAFG DYGLVALEPA WITAQQIEAA RVAMVRHFRR GGKIFIRIFP DKPYTKKPLE VRMGKGKGN VEGYVAVVKP GRVMFEVAGV TEEQAMEALR IAGHKLPIKT KIVRRDAYDE AQ

+
Macromolecule #52: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 13.750148 KDa
SequenceString:
MRHLKSGRKL NRHSSHRLAL YRNQAKSLLT HGRITTTVPK AKELRGFVDH LIHLAKRGDL HARRLVLRDL QDVKLVRKLF DEIAPRYRD RQGGYTRVLK LAERRRGDGA PLALVELVE

+
Macromolecule #53: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 10.998918 KDa
SequenceString:
KFRVRNRIKR TGRLRLSVFR SLKHIYAQII DDEKGVTLVS ASSLALKLKG NKTEVARQVG RALAEKALAL GIKQVAFDRG PYKYHGRVK ALAEGAREGG

+
Macromolecule #54: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 16.289891 KDa
SequenceString:
MNRGALIKLV ESRYVRTDLP EFRPGDTVRV SYKVKEGNRT RIQDFEGIVI RIRRNGFNTT FTVRKVSYGV GVERIFPLHS PLIQKIDIV QRGRARRAKL YFIRNLSDRE IRRKLRADRK RIDQDRAAER AAKEEAQKA

+
Macromolecule #55: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 13.648081 KDa
SequenceString:
PRAKTGVVRR RKHKKILKLA KGYWGLRSKS FRKARETLFA AGNYAYAHRK RRKRDFRRLW IVRINAACRQ HGLNYSTFIH GLKKAGIEV DRKNLADLAV REPQVFAELV ERAKAAQG

+
Macromolecule #56: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.069153 KDa
SequenceString:
MFAIVKTGGK QYRVEPGLKL RVEKLDAEPG ATVELPVLLL GGEKTVVGTP VVEGASVVAE VLGHGRGKKI LVSKFKAKVQ YRRKKGHRQ PYTELLIKEI RG

+
Macromolecule #57: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 12.808055 KDa
SequenceString:
MEAKAIARYV RISPRKVRLV VDLIRGKSLE EARNILRYTN KRGAYFVAKV LESAAANAVN NHDMLEDRLY VKAAYVDEGP ALKRVLPRA RGRADIIKKR TSHITVILGE KHGK

+
Macromolecule #58: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 10.386274 KDa
SequenceString:
TAYDVILAPV LSEKAYAGFA EGKYTFWVHP KATKTEIKNA VETAFKVKVV KVNTLHVRGK KKRLGRYLGK RPDRKKAIVQ VAPGQKIEA LEGL

+
Macromolecule #59: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.181658 KDa
SequenceString:
RVKMHVKKGD TVLVASGKYK GRVGKVKEVL PKKYAVIVEG VNIVKKAVRV SPKYPQGGFI EKEAPLHASK VRPICPACGK PTRVRKKFL ENGKKIRVCA KC

+
Macromolecule #60: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 60 / Number of copies: 462 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #61: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 61 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #62: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 62 / Number of copies: 1 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
Component:
ConcentrationNameFormula
5.0 mMHEPES-KOH (pH 7.5)
10.0 mMAmmonium chlorideNH4CL
10.0 mMMgOAc
50.0 mMpotassium chlorideKCl
0.1 mMEthylenediaminetetraacetic acid (EDTA)C10H16N2O8
6.0 mMBeta-mercaptoethanol2-Mercaptoethanol
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.04 kPa
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 303 K / Instrument: FEI VITROBOT MARK II

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Calibrated defocus max: 2.7 µm / Calibrated defocus min: 0.4 µm / Calibrated magnification: 120443 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 0.0023 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 59000
Specialist opticsSpherical aberration corrector: CS-corrected
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Digitization - Frames/image: 1-7 / Number grids imaged: 1 / Number real images: 949 / Average exposure time: 1.0 sec. / Average electron dose: 45.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 96509
CTF correctionSoftware:
Namedetails
CTFFIND (ver. 4)used for phase calculation
RELION (ver. 1.4)used for phase flipping and amplitude correction
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

4v5f


Details: filtered at 60A
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
Final 3D classificationNumber classes: 4 / Software - Name: RELION (ver. 1.4)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4)
Details: Resolution : 5.36 Resolution (masked and sharpened) : 3.8
Number images used: 32383
FSC plot (resolution estimation)

-
Atomic model buiding 1

Detailseach ribosomal rna and protein was first rigid body fitted in the map using UCSF chimera. The missing componant (mRNA and bL9) were then added and fitted. the quality of the fitting was then assesed and adjust manually, residue per residue using coot. the RNA confomation were ajusted with the ERRASER modul (in Phenix). The final model was refined using phenix real space refinement procedure.
RefinementSpace: REAL / Protocol: RIGID BODY FIT / Target criteria: Cross-correlation coefficient
Output model

PDB-5ot7:
Elongation factor G-ribosome complex captures in the absence of inhibitors.

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