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- EMDB-6780: Small subunit of Toxoplasma gondii ribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-6780
TitleSmall subunit of Toxoplasma gondii ribosome
Map data
Sample
  • Complex: Small subunit of Toxoplasma gondii ribosome
    • RNA: x 1 types
    • Protein or peptide: x 33 types
Function / homology
Function and homology information


chloroplast / ribosomal small subunit assembly / cytosolic small ribosomal subunit / small ribosomal subunit / membrane => GO:0016020 / rRNA binding / ribosome / structural constituent of ribosome / translation / RNA binding ...chloroplast / ribosomal small subunit assembly / cytosolic small ribosomal subunit / small ribosomal subunit / membrane => GO:0016020 / rRNA binding / ribosome / structural constituent of ribosome / translation / RNA binding / zinc ion binding / metal ion binding
Similarity search - Function
: / Ribosomal protein S12e / S27a-like superfamily / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / Ribosomal protein S17e, conserved site / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a ...: / Ribosomal protein S12e / S27a-like superfamily / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / Ribosomal protein S17e, conserved site / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S3, eukaryotic/archaeal / S25 ribosomal protein / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S19A/S15e / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S30 / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein S2, eukaryotic / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S19e / 40S Ribosomal protein S10 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal_S17 N-terminal / Plectin/S10, N-terminal / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal protein S7e / Plectin/S10 domain / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S8e / Ribosomal protein S4, KOW domain / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S4/S9, eukaryotic/archaeal / RS4NT (NUC023) domain / Ribosomal protein S27 / Ribosomal S3Ae family / Ribosomal protein S17e signature. / Ribosomal protein S28e / Ribosomal family S4e / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S4e signature. / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S24e / Ribosomal protein S24e / Ribosomal protein S6e signature. / Ribosomal protein S28e signature. / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein S14/S29 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / 50S ribosomal protein L30e-like / Ubiquitin domain / Ribosomal protein S2 signature 2. / Ribosomal protein S14, conserved site / K Homology domain, type 2 / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S15/S19, conserved site / KH domain / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily
Similarity search - Domain/homology
40S ribosomal protein S3, putative / 40S ribosomal protein S29, putative / Ribosomal protein RPS28 / Ribosomal protein RPS15A / 40S ribosomal protein S24 / Ribosomal protein RPS19 / 40S ribosomal protein S21 / 40S ribosomal protein S13, putative / Ribosomal protein RPS27 / 40s ribosomal protein S14, putative ...40S ribosomal protein S3, putative / 40S ribosomal protein S29, putative / Ribosomal protein RPS28 / Ribosomal protein RPS15A / 40S ribosomal protein S24 / Ribosomal protein RPS19 / 40S ribosomal protein S21 / 40S ribosomal protein S13, putative / Ribosomal protein RPS27 / 40s ribosomal protein S14, putative / Ribosomal protein RPS16 / 40S ribosomal protein S8 / 40s ribosomal protein S20, putative / 40S ribosomal protein S3a / Ribosomal protein RPS23 / 40S ribosomal protein S18, putative / 40S ribosomal protein S11, putative / 40S ribosomal protein S7 / 40S ribosomal protein S26 / 40S ribosomal protein S5, putative / Ribosomal-ubiquitin protein RPS27A / 40S ribosomal protein S9, putative / 40S ribosomal protein S15, putative / 40S ribosomal protein S30 / 40S ribosomal protein S6 / Ribosomal protein RPS10 / 40s ribosomal protein s17, putative / 40S ribosomal protein S2, putative / 40S ribosomal protein S12 / Uncharacterized protein / 40S ribosomal protein S4 / 40S ribosomal protein SA / 40S ribosomal protein S25
Similarity search - Component
Biological speciesToxoplasma gondii (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.35 Å
AuthorsLI Z / Guo Q / Zheng L / Ji Y / Xie Y / Lai D / Lun Z / Suo X / Gao N
CitationJournal: Cell Res / Year: 2017
Title: Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii.
Authors: Zhifei Li / Qiang Guo / Lvqin Zheng / Yongsheng Ji / Yi-Ting Xie / De-Hua Lai / Zhao-Rong Lun / Xun Suo / Ning Gao /
Abstract: As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High- ...As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. With cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, Trichomonas vaginalis and Toxoplasma gondii, at resolution of 3.2-3.4 Å. Although the ribosomal proteins from both pathogens are typical members of eukaryotic families, with a co-evolution pattern between certain species-specific insertions/extensions and neighboring ribosomal RNA (rRNA) expansion segments, the sizes of their rRNAs are sharply different. Very interestingly, rRNAs of T. vaginalis are in size comparable to prokaryotic counterparts, with nearly all the eukaryote-specific rRNA expansion segments missing. These structures facilitate the dissection of evolution path for ribosomal proteins and RNAs, and may aid in design of novel translation inhibitors.
History
DepositionJul 5, 2017-
Header (metadata) releaseAug 30, 2017-
Map releaseAug 30, 2017-
UpdateOct 24, 2018-
Current statusOct 24, 2018Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5xxu
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_6780.png

Downloads & links

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Map

FileDownload / File: emd_6780.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.32 Å
Density
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum-0.11514145 - 0.20913129
Average (Standard dev.)0.000327257 (±0.0049943044)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 422.40002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.321.321.32
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z422.400422.400422.400
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.1150.2090.000

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Supplemental data

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Mask #1

Fileemd_6780_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Small subunit of Toxoplasma gondii ribosome

EntireName: Small subunit of Toxoplasma gondii ribosome
Components
  • Complex: Small subunit of Toxoplasma gondii ribosome
    • RNA: 18S RNA
    • Protein or peptide: Ribosomal protein uS2
    • Protein or peptide: Ribosomal protein eS1
    • Protein or peptide: Ribosomal protein uS5
    • Protein or peptide: Ribosomal protein uS3
    • Protein or peptide: Ribosomal protein eS4
    • Protein or peptide: Ribosomal protein uS7
    • Protein or peptide: Ribosomal protein eS6
    • Protein or peptide: Ribosomal protein eS7
    • Protein or peptide: Ribosomal protein eS8
    • Protein or peptide: Ribosomal protein uS4
    • Protein or peptide: Ribosomal protein eS10
    • Protein or peptide: Ribosomal protein uS17
    • Protein or peptide: Ribosomal protein eS12
    • Protein or peptide: Ribosomal protein uS15
    • Protein or peptide: Ribosomal protein uS11
    • Protein or peptide: Ribosomal protein uS19
    • Protein or peptide: Ribosomal protein uS9
    • Protein or peptide: Ribosomal protein eS17
    • Protein or peptide: Ribosomal protein uS13
    • Protein or peptide: Ribosomal protein eS19
    • Protein or peptide: Ribosomal protein uS10
    • Protein or peptide: Ribosomal protein eS21
    • Protein or peptide: Ribosomal protein uS8
    • Protein or peptide: Ribosomal protein uS12
    • Protein or peptide: Ribosomal protein eS24
    • Protein or peptide: Ribosomal protein eS25
    • Protein or peptide: Ribosomal protein eS26
    • Protein or peptide: Ribosomal protein eS27
    • Protein or peptide: Ribosomal protein eS28
    • Protein or peptide: Ribosomal protein uS14
    • Protein or peptide: Ribosomal protein eS30
    • Protein or peptide: Ribosomal protein eS31
    • Protein or peptide: Ribosomal protein eL41

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Supramolecule #1: Small subunit of Toxoplasma gondii ribosome

SupramoleculeName: Small subunit of Toxoplasma gondii ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Toxoplasma gondii (eukaryote)

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Macromolecule #1: 18S RNA

MacromoleculeName: 18S RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 576.630812 KDa
SequenceString: AACCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUUAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC UUUUAUACGG CUAAACUGC GAAUGGCUCA UUAAAACAGU UAUAGUUUAU UUGAUGGUCU UUACUACAUG GAUAACCGUG GUAAUUCUAU G GCUAAUAC ...String:
AACCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUUAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC UUUUAUACGG CUAAACUGC GAAUGGCUCA UUAAAACAGU UAUAGUUUAU UUGAUGGUCU UUACUACAUG GAUAACCGUG GUAAUUCUAU G GCUAAUAC CUGCGCACAU GCCUCUUCCC CUGGAAGGCA GUGUUUAUUA GAUACAGAAC CAACCCACCU UCCGGUGGUC CU CAGGUGA UUCAUAGUAA CCGAACGGAU CGCGUUGACU UCGGUCUGCG ACGGAUCAUU CAAGUUUCUG ACCUAUCAGC UUU CGACGG UACUGUAAUG GACUACCGUG GCAGUGACGG GUAACGGGGA AUUAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCU AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUGAUUC AGGGAGGUAG UGACAAGAAA UAACA ACAC UGGAAAUUUC AUUUCUAGUG AUUGGAAUGA UAGGAAUCCA AACCCCUUUC AGAGUAACAA UUGGAGGGCA AGUCUG GUG CCAGCAGCCG CGGUAAUUCC AGCUCCAAUA GCGUAUAUUA AAGUUGUUGC AGUUAAAAAG CUCGUAGUUG GAUUUCU GC UGGAAGGCCA GUCCGCCCUC AGGGGUGUGC ACUUGGUGAA UUCUAGCAUC CUUCUGGAUU UCUCCACACU UCAUUGUG U GGAGUUUUUU CCAGGACUUU UACUUUGAGA AAAUUAGAGU GUUUCAAGCA GGCUUGUCGC CUUGAAUACU GCAGCAUGG AAUAAUAAGA UAGGAUUUCG GCCCUAUUUU GUUGGUUUCU AGGACUGAAG UAAUGAUUAA UAGGGACGGU UGGGGGCAUU CGUAUUUAA CUGUCAGAGG UGAAAUUCUU AGAUUUGUUA AAGACGAACU ACUGCGAAAG CAUUUGCCAA AGAUGUUUUC A UUAAUCAA GAACGAAAGU UAGGGGCUCG AAGACGAUCA GAUACCGUCG UAGUCUUAAC CAUAAACUAU GCCGACUAGA GA UAGGAAA ACGUCAUGCU UGACUUCUCC UGCACCUUAU GAGAAACCAA AGUCUUUGGG UUCUGGGGGG AGUAUGGUCG CAA GGCUGA AACUUAAAGG AAUUGACGGA AGGGCACCAC CAGGCGUGGA GCCUGCGGCU UAAUUUGACU CAACACGGGG AAAC UCACC AGGUCCAGAC AUAGGAAGGA UUGACAGAUU GAUAGCUCUU UCUUGAUUCU AUGGGUGGUG GUGCAUGGCC GUUCU UAGU UGGUGGAGUG AUUUGUCUGG UUAAUUCCGU UAACGAACGA GACCUUAACC UGCUAAAUAG GAUCAGGAAC UUCGUG UUC UUGUAUCACU UCUUAGAGGG ACUUUGCGUG UCUAACGCAA GGAAGUUUGA GGCAAUAACA GGUCUGUGAU GCCCUUA GA UGUUCUGGGC UGCACGCGCG CUACACUGAU GCAUCCAACG AGUUUAUAAC CUUGGCCGAU AGGUCUAGGU AAUCUUGU G AGUAUGCAUC GUGAUGGGGA UAGAUUAUUG CAAUUAUUAA UCUUCAACGA GGAAUGCCUA GUAGGCGCAA GUCAGCACG UUGCGCCGAU UACGUCCCUG CCCUUUGUAC ACACCGCCCG UCGCUCCUAC CGAUUGAGUG UUCCGGUGAA UUAUUCGGAC CGUUUUGUG GCGCGUUCGU GCCCGAAAUG GGAAGUUUUG UGAACCUUAA CACUUAGAGG AAGGAGAAGU CGUAACAAGG U UUCCGUAG GUGAACCUGC GGAAGGAUCA UUC

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Macromolecule #2: Ribosomal protein uS2

MacromoleculeName: Ribosomal protein uS2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 31.546902 KDa
SequenceString: MAADKQAKLS LQEDSIAKML ICKAHIGTKN VEHKMRPYVF KSTSEGIHLI NLAKTWEKIL MAARVIVAIE NPADVLVISA RPYGSRAVL KFSQYVGAQA IAGRWTPGML TNQITQKFME PRLLIVTDPR TDAQAVRESA YANVPVIALC DTDSPLEHVD I CIPCNNKG ...String:
MAADKQAKLS LQEDSIAKML ICKAHIGTKN VEHKMRPYVF KSTSEGIHLI NLAKTWEKIL MAARVIVAIE NPADVLVISA RPYGSRAVL KFSQYVGAQA IAGRWTPGML TNQITQKFME PRLLIVTDPR TDAQAVRESA YANVPVIALC DTDSPLEHVD I CIPCNNKG KESIALMYWL LAREVLYLRG ELPSRSVPWD VMVDMFLWRD PEEFERKERL EDEEAAPHTA QADATQWGAD AA AADWKQA GADWTGPAAG GAGGEWDQSA VAAEGEWGGG RPGVAVGEPW

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Macromolecule #3: Ribosomal protein eS1

MacromoleculeName: Ribosomal protein eS1 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 29.425625 KDa
SequenceString: MAIGKNKRMS KGKKGGKKKI GDPFLKKEWY DLKAPTLFNV RNFGKTLVTK SQGTKLAVDG LRGRVYEVNL ADLNNDEDQG FRKIKLCCE DIQGRNCLTD FHGMDMTRDK ICSLVRKCHS LIEAFVDVTT LDGYTLRMFC IAFTKRRPEQ VKSTCYAQTS Q IRAIRKKM ...String:
MAIGKNKRMS KGKKGGKKKI GDPFLKKEWY DLKAPTLFNV RNFGKTLVTK SQGTKLAVDG LRGRVYEVNL ADLNNDEDQG FRKIKLCCE DIQGRNCLTD FHGMDMTRDK ICSLVRKCHS LIEAFVDVTT LDGYTLRMFC IAFTKRRPEQ VKSTCYAQTS Q IRAIRKKM MTIMSAEASK CQLRDLVKKF IPESIGKDIE NACKGIFPLQ NVFIRKVKML KKPKFDLTKL MELHGEAEDV GR KVQAESG EAQNTLTAET KA

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Macromolecule #4: Ribosomal protein uS5

MacromoleculeName: Ribosomal protein uS5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 29.385004 KDa
SequenceString: MAERGSFGRG FGRGGGRGRG RGRGRGRGGA EDDLKDWVPI TKLGRLVRDG KISSIEEIYL FSLPIKEYQI IDHFFQPGNC AAPLKDDVM KIMPVQKQTR AGQRTRFKAF VAVGDSNGHC GLGVKCAKEV ATAIRGAIIA AKLSLVPVRR GYWGNKIGEP H TVPMKVSG ...String:
MAERGSFGRG FGRGGGRGRG RGRGRGRGGA EDDLKDWVPI TKLGRLVRDG KISSIEEIYL FSLPIKEYQI IDHFFQPGNC AAPLKDDVM KIMPVQKQTR AGQRTRFKAF VAVGDSNGHC GLGVKCAKEV ATAIRGAIIA AKLSLVPVRR GYWGNKIGEP H TVPMKVSG RCGSVRVRLI PAPRGTHIVG APTTKKILGF AGIKDCFSNS KGSTKTRGNF MKALFDALSQ TYGYLTPELW TP TVYTKSP YQEWSDYLAR TTKDSAVSRP LA

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Macromolecule #5: Ribosomal protein uS3

MacromoleculeName: Ribosomal protein uS3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 26.117539 KDa
SequenceString: MASTGNTCKK RKFVKDGVFQ AELNEFLSCT LSEDGYSGVE VRVTPIRTEI IIRATRTREV LGDKGRRIRE LTSVVQKRFG FAPDSVELF AERVENRGLC AMAQAESLRY KLLKGLAVRR ACYGVLRHIM ESGAKGCEVV VSGKLRAQRA KSMKFKDGYL I STGEPSKM ...String:
MASTGNTCKK RKFVKDGVFQ AELNEFLSCT LSEDGYSGVE VRVTPIRTEI IIRATRTREV LGDKGRRIRE LTSVVQKRFG FAPDSVELF AERVENRGLC AMAQAESLRY KLLKGLAVRR ACYGVLRHIM ESGAKGCEVV VSGKLRAQRA KSMKFKDGYL I STGEPSKM FVDQAIRSVQ LRQGVLGVRV KIMLPHDPEG KRGPANPLPD TIIVMDPKPE IPVVQPEEMD EGVLGPM

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Macromolecule #6: Ribosomal protein eS4

MacromoleculeName: Ribosomal protein eS4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 30.204713 KDa
SequenceString: MARGPRKHLK RIAAPHHWML DKLTGHYAPR PSPGPHKLRE SAPLVVLLRN RLRYALTYRE VMMIVMQRLI KVDNKVRTDQ CYPAGFMDV ISIEKTKENF RMLFDTKGRF VPHPIREEEA SYKLCRVKKV VVGPKGVPAL ITHDGRTMRY PHPSIKAHDC I RLDLNTGK ...String:
MARGPRKHLK RIAAPHHWML DKLTGHYAPR PSPGPHKLRE SAPLVVLLRN RLRYALTYRE VMMIVMQRLI KVDNKVRTDQ CYPAGFMDV ISIEKTKENF RMLFDTKGRF VPHPIREEEA SYKLCRVKKV VVGPKGVPAL ITHDGRTMRY PHPSIKAHDC I RLDLNTGK IVDTLKFEAG NMAMVTGGHN VGRVGVIVHR ERHLGGFDII HLRDAKNNEF ATRISNVFVI GKGEKAWISL PK EKGIRLS IMENRQVLLK KQQMNN

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Macromolecule #7: Ribosomal protein uS7

MacromoleculeName: Ribosomal protein uS7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 21.650238 KDa
SequenceString: MATEAKLFGR WSYDDVNVSD LSLVDYIAVK DKACVFVPHT AGRYQKKRFR KAMCPIVERL VNSMMMHGRN NGKKTLSVRI VRHAFEIIH LMTDKNPIQV FVNAVENGGP REDSTRIGSA GVVRRQAVDV SPLRRVNQAI YLICTGARLA AFRNIKTIAE C LADEIMNC ...String:
MATEAKLFGR WSYDDVNVSD LSLVDYIAVK DKACVFVPHT AGRYQKKRFR KAMCPIVERL VNSMMMHGRN NGKKTLSVRI VRHAFEIIH LMTDKNPIQV FVNAVENGGP REDSTRIGSA GVVRRQAVDV SPLRRVNQAI YLICTGARLA AFRNIKTIAE C LADEIMNC AKESSNAYAI KKKDEIERVA KANR

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Macromolecule #8: Ribosomal protein eS6

MacromoleculeName: Ribosomal protein eS6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 29.027504 KDa
SequenceString: MKLNLANPQA GMQKTVEVDD EKKLLPFFEK RMGAEVAGDS IGDEFKGYIF RISGGNDKQG FPMMQGVLVN HRVRLLFRKG MKCYRQRRT GEKKRKSVRG SIVGPDLAVL NLVLVKKGPE TIPGLTDAER PRRLGPKRAN NIRKLFNLSK DQDVRKYVVR R QIEGKKKT ...String:
MKLNLANPQA GMQKTVEVDD EKKLLPFFEK RMGAEVAGDS IGDEFKGYIF RISGGNDKQG FPMMQGVLVN HRVRLLFRKG MKCYRQRRT GEKKRKSVRG SIVGPDLAVL NLVLVKKGPE TIPGLTDAER PRRLGPKRAN NIRKLFNLSK DQDVRKYVVR R QIEGKKKT KAPKIQRLIT KTRVQRKRAY LAKQRRSVIK SREEAKAYKD VLSAYKHELR KKRADVVAKK KAQAAAAPKP AE VKVVKAK AATAKKGGK

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Macromolecule #9: Ribosomal protein eS7

MacromoleculeName: Ribosomal protein eS7 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 22.631727 KDa
SequenceString: MMNFKKKIVK PQGVEPTEIE NEVAKCLFDI ETSSQSDLKG DVRHLVISSV KEVEVPQGRK KAFVVFVPHA VYQKTVKKIQ GRLVQELEK KLKKHVVLVA QRTMLPLDFK RKGLKVRPRS RTLTAVQDGM LEDIVSPTEI VGKRMRVRVD NTKMLKVFLD P RDKQKDNI ...String:
MMNFKKKIVK PQGVEPTEIE NEVAKCLFDI ETSSQSDLKG DVRHLVISSV KEVEVPQGRK KAFVVFVPHA VYQKTVKKIQ GRLVQELEK KLKKHVVLVA QRTMLPLDFK RKGLKVRPRS RTLTAVQDGM LEDIVSPTEI VGKRMRVRVD NTKMLKVFLD P RDKQKDNI EDKLETFAAV YKKLTNKDAV FSFPQHVY

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Macromolecule #10: Ribosomal protein eS8

MacromoleculeName: Ribosomal protein eS8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 23.615604 KDa
SequenceString: MGISRDRRHK HRRTGGRMPV HQKKRKFELG RPPAMTKLGP KRVRPVRCRG GNMKYRALRL DTGNYSWSSQ KIAKKTRLLG VVYNASNNE LVRTNTLVKN SIVAIDATPF KAWFRSFYGL DLGKKKAGQE EEGSRKVSKH TLCLQNKHRQ QGVPKLDPLL E EQFNTGRL ...String:
MGISRDRRHK HRRTGGRMPV HQKKRKFELG RPPAMTKLGP KRVRPVRCRG GNMKYRALRL DTGNYSWSSQ KIAKKTRLLG VVYNASNNE LVRTNTLVKN SIVAIDATPF KAWFRSFYGL DLGKKKAGQE EEGSRKVSKH TLCLQNKHRQ QGVPKLDPLL E EQFNTGRL LACISSRPGQ SGRADGYILE GEELMFYKRK MDKKKHK

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Macromolecule #11: Ribosomal protein uS4

MacromoleculeName: Ribosomal protein uS4 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 21.731396 KDa
SequenceString:
MTKSYRTYSK TARSPKRPFE KERLDQEMKL LGEYGLKNKR EVWRVQYALA KIRSAARELL TLEEKDPRRV FQGTALLRRM VRLGLLGES EQKLDYVLGL TVAKFLERRL QTKVFKLGLA KSIHHARVLI RQRHIRVGKH IVDIPSFMVR VDSEKHVDYA I TSPFGGGR PGRVKRRSLK GGNAEGGDDE

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Macromolecule #12: Ribosomal protein eS10

MacromoleculeName: Ribosomal protein eS10 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 17.428098 KDa
SequenceString:
MTVPGMKFSL IPKANRKAIY EYLFKEGVIV VQKNGKIERH PEVPVPNLHV MMTLRSLQTK KFVEEKFNWQ HNYYFLTNEG IEFLRTYLH LPPTVFPSTL TKKSTGRPTR GAGEGMGEGG DMNGDWRPRG GFGRGRGRMG DRPDRAERAP RYA

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Macromolecule #13: Ribosomal protein uS17

MacromoleculeName: Ribosomal protein uS17 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 18.715984 KDa
SequenceString:
MATADVQTER AFQKQDGVSS LGRLHLTGQK KRTQRYWKDV GLGFQTPRLA KESKYVDKKC PFTGNVSIRG RVIKGMVIST KMKRAVVIR RNYLHFVPKY SRFEKRHKNV TCHLSPCFEQ VKEGDIVTAG QCRPLSKTIR FNVLKVEKNQ VFGNVRKQFR L F

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Macromolecule #14: Ribosomal protein eS12

MacromoleculeName: Ribosomal protein eS12 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 15.419675 KDa
SequenceString:
MSDVESVADE VVAPVEEEEE VKDLMTAIRK VLKNALIHDG LVRGLHEVAK ALDAKKAQVC FLSESCSEPA YKKLVQGLCK EHGIPLLDV TDSKELGEWA GLCKVDKDGT ARKVVGASCV CVTDFGEESE ALTFLQNHIK TLS

+
Macromolecule #15: Ribosomal protein uS15

MacromoleculeName: Ribosomal protein uS15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 17.211363 KDa
SequenceString:
MGRMYGPGKG MSASALPWRR KPPTWLKIKP SDVEEHIAKL AKKGQTPSQI GVTLRDSFGV PQVKSVTGNK ILRILKLQGL APELPEDLY YLIKKAVSVR KHLERNRKDK DAKFRLILVE SRIHRLARYY KRTKQLPATW KYQSATASAL VA

+
Macromolecule #16: Ribosomal protein uS11

MacromoleculeName: Ribosomal protein uS11 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 16.330598 KDa
SequenceString:
MAPKKSAKAA TGEEGEAQGS GLGPATRDGE IVFGVAHIYA SFNDTFIHVT DLSGRETLVR VTGGMKVKAD RDESSPYAAM MAAADVAAR CKELGISAVH VKVRAAGGTR SKTPGPGAQS ALRALARSGL RIGRIEDVTP IPTDSTRRKS GRRGRRL

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Macromolecule #17: Ribosomal protein uS19

MacromoleculeName: Ribosomal protein uS19 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 17.190223 KDa
SequenceString:
MADAGDAAAN QPKRRTFRTF SYRGVELDKL LDMKMEDLLE LFRARQRRKF QRGIKRKATT LMKKLRISKK NCAFGEKPEP VNTHLRDLV VVPEMIGSVV GVYNGKQFIN VEIKPEMVGY YLGEFSISYK PVRHGKPGIG ATHSSRFIPL K

+
Macromolecule #18: Ribosomal protein uS9

MacromoleculeName: Ribosomal protein uS9 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 16.587488 KDa
SequenceString:
MADIGKRPKK RVQTFGRKKN AVAVALCTQG KGLLRVNGCP LEHLQPEALK VKAFEPLLLL GKERFQDVDI RVRVSGGGYV AQIYAIRQA IAKAVVAFNQ KYVDEATKKE VRDILVAYDR SLIVADPRRC EPKKFGGPGA RARYQKSYR

+
Macromolecule #19: Ribosomal protein eS17

MacromoleculeName: Ribosomal protein eS17 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 15.256675 KDa
SequenceString:
MGRVRTRTVK RAARQIVEKY YAKLTLDFQI NKKISEEVAV IPSKRMRNRV AGFVTHLMKR IQKGPVRGIS LKLQEEERER RMDFVPERS EVDVATITVD QDTADMLRAL DINLPNVVVP SAGRPSNNQN RRN

+
Macromolecule #20: Ribosomal protein uS13

MacromoleculeName: Ribosomal protein uS13 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 17.761662 KDa
SequenceString:
MSIQVSNNQD FQHILRILNT NVDGREKVVV ALTAIRGIGR RVSNLICKKA DIDTNKRAGE LTADEISKVV AIASTPQQFK IPTWFLNRQ KDVKDGKHKH VVANGLDTVW REDLERLKKM RLHRGLRHYW GLKVRGQHTK TTGRHGRTVG VAKKKGC

+
Macromolecule #21: Ribosomal protein eS19

MacromoleculeName: Ribosomal protein eS19 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 18.414312 KDa
SequenceString:
MSNPAYLYET PLETRVHTVK DVKADAFIAL YANHLKKRGR FQLPKWIDYV KTAHAKELAP YDPDWLYIRA AAILRHLYIR PDCGVGGFR KVFSCRQRRG VRPNHTSKAS GKIIRYILQQ FEEMGLVESD PEKPGRRLTK NGQRELDVIA RQCAKPAVAS D

+
Macromolecule #22: Ribosomal protein uS10

MacromoleculeName: Ribosomal protein uS10 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 25.943293 KDa
SequenceString: MHVFSDVCAR PGSPEKTDRR RIFCAKVPTK RRKKCVFCVF EASPELCAFS ALPPHVTRDN RCLSVSLSVS RLPSSSRALP PAPASSSHS SCPRSQPLPG LLLFLKSSEG EPQKAKMSKL MKGGLEGEEQ RLHRIRITLT SKDLKSIERV CTELINGAKE K NLQVAGPV ...String:
MHVFSDVCAR PGSPEKTDRR RIFCAKVPTK RRKKCVFCVF EASPELCAFS ALPPHVTRDN RCLSVSLSVS RLPSSSRALP PAPASSSHS SCPRSQPLPG LLLFLKSSEG EPQKAKMSKL MKGGLEGEEQ RLHRIRITLT SKDLKSIERV CTELINGAKE K NLQVAGPV RLPVKTLRIT TRKSPCGEGT NTWDRFELRI YKRLIDLHSP SDVVKQITSI NIDPGVEVEV TIIDM

+
Macromolecule #23: Ribosomal protein eS21

MacromoleculeName: Ribosomal protein eS21 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 9.049265 KDa
SequenceString:
MQNDEGRIVD LYIPRKCSAT GRLIPAKEHG AVQINVGQVD EHGRYTGEYH AVAISGAVRQ RGESDACLNR LMHEKGLLSF AK

+
Macromolecule #24: Ribosomal protein uS8

MacromoleculeName: Ribosomal protein uS8 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 14.973676 KDa
SequenceString:
MVRMNVLADC LKTLVNAEKR GRRQVLIRPS SKVIIKFLQC MQRRGYIGDF EIVDDHRAGK IVIELLGRIN KCGVISPRFD IQVDKIEQF CSNLLPSRQF GHLVLTTAYG IMDHEEARRK HTGGKILGYF F

+
Macromolecule #25: Ribosomal protein uS12

MacromoleculeName: Ribosomal protein uS12 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 15.837605 KDa
SequenceString:
MGKPNGIRTA RKLRSRRRVQ KWADKTYKKA HLGTRWKANP FGGSSHAKGI VVEKLGIEAK QPNSAIRKCV RVQLIKNGKK ITAFVPRDG SLNYIDENDE VLVAGFGRSG HAVGDIPGVR FKVVKVAGAS LLALFKEKKE KPRT

+
Macromolecule #26: Ribosomal protein eS24

MacromoleculeName: Ribosomal protein eS24 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 15.23091 KDa
SequenceString:
MADSGAFSLR FRKLKTNPLL QRKQFGVDIL HPSRGSVSKK ELVEKIAKQF RVSDSQSIVV FGLKTAFGGG RSSGFAMIYD NPNAAKKFE NRFRLVRLGY VEAKPTKGRR AYKELKNRRK KVRGKEKAKC SGAAKK

+
Macromolecule #27: Ribosomal protein eS25

MacromoleculeName: Ribosomal protein eS25 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 18.707014 KDa
SequenceString:
MRELRRLYGY MFRKDRQFFE IARFLIRVNS RVPVPAYGFQ HVSLCFVPVC RFTMAPKEKK TKEQIAAAAA AGSRKNKKKW SKGKSKDKL NHSVLFDKAT YDKLLADIPK ARLITPHTVC ERLKVNASLA RQAIRYLKDQ GLIKIVGEHH HSQYIYTRNT S A

+
Macromolecule #28: Ribosomal protein eS26

MacromoleculeName: Ribosomal protein eS26 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 12.75363 KDa
SequenceString:
MPTKRRNGGR AKHGRGHSAV VRCSNCGRSC PKDKAIKRFN VRNIVDASSQ RDLREASVYN TYTLPKLYIK QCYCVSCAIH SRVVRVRSV EQRKVRENPQ RRPQGGQQGQ GNN

+
Macromolecule #29: Ribosomal protein eS27

MacromoleculeName: Ribosomal protein eS27 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 9.10585 KDa
SequenceString:
MEIDLLHPDP KVEASKHKLK RLIPNPNSFF MDVKCPGCLQ ITTVFSHAQT VVLCGSCNIM LCQPTGGKCK LTEGCSFRKK VE

+
Macromolecule #30: Ribosomal protein eS28

MacromoleculeName: Ribosomal protein eS28 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 7.601858 KDa
SequenceString:
MEQPKLAKVE KVLGRTGSRG GVLQVRVTFM ADETDLAGRS LIRNVKGPVR EGDILALLET EREARRLR

+
Macromolecule #31: Ribosomal protein uS14

MacromoleculeName: Ribosomal protein uS14 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 6.533824 KDa
SequenceString:
MTNLFNTRPK KFGPGSRQCR VCFNRHGMVR KYGLMMCRQC FRERANQIGF VKYR

+
Macromolecule #32: Ribosomal protein eS30

MacromoleculeName: Ribosomal protein eS30 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 6.6198 KDa
SequenceString:
MGKVHGSLAR AGKVKNQTPK VAKKEKKKPP TGRAKKRQQF NRRFTTSVGR KRGPNAQTQ

+
Macromolecule #33: Ribosomal protein eS31

MacromoleculeName: Ribosomal protein eS31 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 17.353152 KDa
SequenceString:
MQIFVLSVGG DTLALEVAPT STIRDVKEQL QARQGIEADD QRLCFGLHAL EDDETLGELG VEEECTLYQS LELLGAGKKR KKKTYTKPK KQKHKKKKVK LAVLKFYKVD GNDKVTRLRK ECPRETCGAG VFMAAHKNRT YCGRCGLTYI LNAEE

+
Macromolecule #34: Ribosomal protein eL41

MacromoleculeName: Ribosomal protein eL41 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 5.120324 KDa
SequenceString:
MAHGASRYKK ARAKMRWKWK KKRTRRLQRK RRKMRQRSR

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 2.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: ANGULAR RECONSTITUTION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.35 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 108162
FSC plot (resolution estimation)

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