Author results

6OMF
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CRYOEM STRUCTURE OF SIGMAS-TRANSCRIPTION INITIATION COMPLEX WITH ACTIVATOR CRL
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Jaramillo Cartagena, A., Darst, S.A., Campbell, E.A.
Deposit date:2019-04-18
Release date:2019-08-28
Last modified:2019-10-02
Method:ELECTRON MICROSCOPY (3.26 Å)
Cite:Structural basis for transcription activation by Crl through tethering of sigmaSand RNA polymerase.
Proc.Natl.Acad.Sci.USA, 116, 2019
6N62
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ESCHERICHIA COLI RNA POLYMERASE SIGMA70-HOLOENZYME BOUND TO UPSTREAM FORK PROMOTER DNA
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Braffman, N., Hauver, J., Campbell, E.A., Darst, S.A.
Deposit date:2018-11-24
Release date:2019-01-09
Last modified:2019-02-06
Method:X-RAY DIFFRACTION (3.803 Å)
Cite:Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin.
Proc. Natl. Acad. Sci. U.S.A., 116, 2019
6N61
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ESCHERICHIA COLI RNA POLYMERASE SIGMA70-HOLOENZYME BOUND TO UPSTREAM FORK PROMOTER DNA AND CAPISTRUIN
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Braffman, N., Hauver, J., Campbell, E.A., Darst, S.A.
Deposit date:2018-11-24
Release date:2019-01-09
Last modified:2019-02-06
Method:X-RAY DIFFRACTION (3.253 Å)
Cite:Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin.
Proc. Natl. Acad. Sci. U.S.A., 116, 2019
6N60
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ESCHERICHIA COLI RNA POLYMERASE SIGMA70-HOLOENZYME BOUND TO UPSTREAM FORK PROMOTER DNA AND MICROCIN J25 (MCCJ25)
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Braffman, N., Hauver, J., Campbell, E.A., Darst, S.A.
Deposit date:2018-11-23
Release date:2019-01-09
Last modified:2019-02-27
Method:X-RAY DIFFRACTION (3.68 Å)
Cite:Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin.
Proc. Natl. Acad. Sci. U.S.A., 116, 2019
6M7J
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MYCOBACTERIUM TUBERCULOSIS RNAP WITH RBPA/US FORK AND CORALLOPYRONIN
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J.
Deposit date:2018-08-20
Release date:2018-11-21
Last modified:2019-01-30
Method:ELECTRON MICROSCOPY (4.4 Å)
Cite:Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.
Nature, 565, 2019
6EEC
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MYCOBACTERIUM TUBERCULOSIS RNAP PROMOTER UNWINDING INTERMEDIATE COMPLEX WITH RBPA/CARD AND AP3 PROMOTER CAPTURED BY CORALLOPYRONIN
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J.
Deposit date:2018-08-13
Release date:2018-11-21
Last modified:2019-01-30
Method:ELECTRON MICROSCOPY (3.55 Å)
Cite:Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.
Nature, 565, 2019
6EE8
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MYCOBACTERIUM TUBERCULOSIS RNAP PROMOTER UNWINDING INTERMEDIATE COMPLEX WITH RBPA/CARD AND AP3 PROMOTER
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J.
Deposit date:2018-08-13
Release date:2018-11-21
Last modified:2019-01-30
Method:ELECTRON MICROSCOPY (3.92 Å)
Cite:Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.
Nature, 565, 2019
6EDT
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MYCOBACTERIUM TUBERCULOSIS RNAP OPEN PROMOTER COMPLEX WITH RBPA/CARD AND AP3 PROMOTER
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J.
Deposit date:2018-08-10
Release date:2018-11-21
Last modified:2019-01-30
Method:ELECTRON MICROSCOPY
Cite:Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.
Nature, 565, 2019
6DCF
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CRYSTAL STRUCTURE OF A MYCOBACTERIUM SMEGMATIS TRANSCRIPTION INITIATION COMPLEX WITH RIFAMPICIN-RESISTANT RNA POLYMERASE AND BOUND TO KANGLEMYCIN A
Descriptor:RNA polymerase-binding protein RbpA, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ...
Authors:Lilic, M., Darst, S.A., Campbell, E.A.
Deposit date:2018-05-06
Release date:2018-09-05
Last modified:2019-03-20
Method:X-RAY DIFFRACTION (3.45 Å)
Cite:Rifamycin congeners kanglemycins are active against rifampicin-resistant bacteria via a distinct mechanism.
Nat Commun, 9, 2018
6CCV
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CRYSTAL STRUCTURE OF A MYCOBACTERIUM SMEGMATIS RNA POLYMERASE TRANSCRIPTION INITIATION COMPLEX WITH INHIBITOR RIFAMPICIN
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Lilic, M., Darst, S.A., Campbell, E.A.
Deposit date:2018-02-07
Release date:2018-08-15
Last modified:2019-05-15
Method:X-RAY DIFFRACTION (3.05 Å)
Cite:Rifamycin congeners kanglemycins are active against rifampicin-resistant bacteria via a distinct mechanism.
Nat Commun, 9, 2018
6CCE
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CRYSTAL STRUCTURE OF A MYCOBACTERIUM SMEGMATIS RNA POLYMERASE TRANSCRIPTION INITIATION COMPLEX WITH INHIBITOR KANGLEMYCIN A
Descriptor:RNA polymerase-binding protein RbpA, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ...
Authors:Lilic, M., Darst, S.A., Campbell, E.A.
Deposit date:2018-02-07
Release date:2018-08-15
Last modified:2019-04-24
Method:X-RAY DIFFRACTION (3.05 Å)
Cite:Rifamycin congeners kanglemycins are active against rifampicin-resistant bacteria via a distinct mechanism.
Nat Commun, 9, 2018
6C06
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MYCOBACTERIUM TUBERCULOSIS RNAP HOLO/RBPA/FIDAXOMICIN
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J., Lilic, M.
Deposit date:2017-12-27
Release date:2018-03-28
Method:ELECTRON MICROSCOPY (5.15 Å)
Cite:Fidaxomicin jamsMycobacterium tuberculosisRNA polymerase motions needed for initiation via RbpA contacts.
Elife, 7, 2018
6C05
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MYCOBACTERIUM TUBERCULOSIS RNAP HOLO/RBPA IN RELAXED STATE
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J., Lilic, M.
Deposit date:2017-12-27
Release date:2018-03-28
Method:ELECTRON MICROSCOPY (5.15 Å)
Cite:Fidaxomicin jamsMycobacterium tuberculosisRNA polymerase motions needed for initiation via RbpA contacts.
Elife, 7, 2018
6C04
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MTB RNAP HOLO/RBPA/DOUBLE FORK DNA -CLOSED CLAMP
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J., Lilic, M.
Deposit date:2017-12-27
Release date:2018-03-28
Method:ELECTRON MICROSCOPY (3.27 Å)
Cite:Fidaxomicin jamsMycobacterium tuberculosisRNA polymerase motions needed for initiation via RbpA contacts.
Elife, 7, 2018
6BZO
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MTB RNAP HOLO/RBPA/FIDAXOMICIN/UPSTREAM FORK DNA
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J.
Deposit date:2017-12-25
Release date:2018-03-28
Last modified:2019-04-24
Method:ELECTRON MICROSCOPY (3.38 Å)
Cite:Fidaxomicin jamsMycobacterium tuberculosisRNA polymerase motions needed for initiation via RbpA contacts.
Elife, 7, 2018
6ALH
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CRYOEM STRUCTURE OF E.COLI RNA POLYMERASE ELONGATION COMPLEX
Descriptor:DNA (5'-D(*GP*GP*GP*CP*TP*AP*AP*TP*GP*AP*CP*GP*GP*CP*GP*AP*AP*TP*AP*CP*CP*C)-3'), DNA (29-MER), RNA (5'-R(P*CP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3'), ...
Authors:Kang, J.Y., Darst, S.A.
Deposit date:2017-08-07
Release date:2017-08-16
Last modified:2018-07-18
Method:ELECTRON MICROSCOPY (4.4 Å)
Cite:Structural basis of transcription arrest by coliphage HK022 Nun in anEscherichia coliRNA polymerase elongation complex.
Elife, 6, 2017
6ALG
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CRYOEM STRUCTURE OF HK022 NUN - E.COLI RNA POLYMERASE ELONGATION COMPLEX
Descriptor:DNA (29-MER), RNA (5'-R(P*CP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3'), DNA-directed RNA polymerase subunit alpha, ...
Authors:Kang, J.Y., Darst, S.A.
Deposit date:2017-08-07
Release date:2017-08-16
Last modified:2018-07-18
Method:ELECTRON MICROSCOPY (3.7 Å)
Cite:Structural basis of transcription arrest by coliphage HK022 Nun in anEscherichia coliRNA polymerase elongation complex.
Elife, 6, 2017
6ALF
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CRYOEM STRUCTURE OF CROSSLINKED E.COLI RNA POLYMERASE ELONGATION COMPLEX
Descriptor:DNA (5'-D(*GP*GP*GP*CP*TP*AP*AP*TP*GP*AP*CP*GP*GP*CP*GP*AP*AP*TP*AP*CP*CP*C)-3'), DNA (29-MER), RNA (5'-R(P*AP*GP*CP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3'), ...
Authors:Kang, J.Y., Darst, S.A.
Deposit date:2017-08-07
Release date:2017-08-16
Last modified:2018-07-18
Method:ELECTRON MICROSCOPY (4.1 Å)
Cite:Structural basis of transcription arrest by coliphage HK022 Nun in anEscherichia coliRNA polymerase elongation complex.
Elife, 6, 2017
5VT0
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ESCHERICHIA COLI 6S RNA DERIVATIVE IN COMPLEX WITH ESCHERICHIA COLI RNA POLYMERASE SIGMA70-HOLOENZYME
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Chen, J., Darst, S.A.
Deposit date:2017-05-15
Release date:2017-11-01
Last modified:2017-12-06
Method:ELECTRON MICROSCOPY (3.78 Å)
Cite:6S RNA Mimics B-Form DNA to Regulate Escherichia coli RNA Polymerase.
Mol. Cell, 68, 2017
5VI8
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STRUCTURE OF A MYCOBACTERIUM SMEGMATIS TRANSCRIPTION INITIATION COMPLEX WITH AN UPSTREAM-FORK PROMOTER FRAGMENT
Descriptor:RNA polymerase-binding protein RbpA, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ...
Authors:Hubin, E.A., Campbell, E.A., Darst, S.A.
Deposit date:2017-04-14
Release date:2017-04-26
Last modified:2019-01-30
Method:X-RAY DIFFRACTION (2.76 Å)
Cite:Structural insights into the mycobacteria transcription initiation complex from analysis of X-ray crystal structures.
Nat Commun, 8, 2017
5VI5
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STRUCTURE OF MYCOBACTERIUM SMEGMATIS TRANSCRIPTION INITIATION COMPLEX WITH A FULL TRANSCRIPTION BUBBLE
Descriptor:DNA (49-MER), DNA (44-MER), RNA (5'-R(*UP*CP*GP*A)-3'), ...
Authors:Darst, S.A., Campbell, E.A., Lilic, M.
Deposit date:2017-04-14
Release date:2017-07-26
Method:X-RAY DIFFRACTION (3.196 Å)
Cite:Structural insights into the mycobacteria transcription initiation complex from analysis of X-ray crystal structures.
Nat Commun, 8, 2017
5UI8
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STRUCTURE OF SIGMAN-HOLOENZYME
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Darst, S.A., Campbell, E.A.
Deposit date:2017-01-13
Release date:2017-02-22
Last modified:2017-09-13
Method:X-RAY DIFFRACTION (3.76 Å)
Cite:Crystal structure of Aquifex aeolicus sigma (N) bound to promoter DNA and the structure of sigma (N)-holoenzyme.
Proc. Natl. Acad. Sci. U.S.A., 114, 2017
5UI5
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CRYSTAL STRUCTURE OF AQUIFEX AEOLICUS SIGMAN BOUND TO PROMOTER DNA
Descriptor:DNA (31-MER), DNA (30-MER), RNA polymerase sigma factor RpoN
Authors:Darst, S.A., Campbell, E.A., Rajashankar, K.
Deposit date:2017-01-12
Release date:2017-02-22
Last modified:2017-09-13
Method:X-RAY DIFFRACTION (3.4 Å)
Cite:Crystal structure of Aquifex aeolicus sigma (N) bound to promoter DNA and the structure of sigma (N)-holoenzyme.
Proc. Natl. Acad. Sci. U.S.A., 114, 2017
5TW1
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CRYSTAL STRUCTURE OF A MYCOBACTERIUM SMEGMATIS TRANSCRIPTION INITIATION COMPLEX WITH RBPA
Descriptor:RNA polymerase-binding protein RbpA, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ...
Authors:Hubin, E.A., Darst, S.A., Campbell, E.A.
Deposit date:2016-11-10
Release date:2017-01-18
Last modified:2017-09-20
Method:X-RAY DIFFRACTION (2.76 Å)
Cite:Structure and function of the mycobacterial transcription initiation complex with the essential regulator RbpA.
Elife, 6, 2017
4XLS
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CRYSTAL STRUCTURE OF T. AQUATICUS TRANSCRIPTION INITIATION COMPLEX WITH CARD CONTAINING UPSTREAM FORK PROMOTER.
Descriptor:DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Bae, B., Darst, S.A.
Deposit date:2015-01-13
Release date:2015-09-23
Last modified:2017-11-22
Method:X-RAY DIFFRACTION (4.01 Å)
Cite:CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex.
Elife, 4, 2015
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