8UQL
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![BU of 8uql by Molmil](/molmil-images/mine/8uql) | Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome | Descriptor: | 16S rRNA, 23S rRNA, 30S ribosomal protein S1, ... | Authors: | Molodtsov, V, Wang, C, Ebright, R.H. | Deposit date: | 2023-10-24 | Release date: | 2024-05-29 | Last modified: | 2024-08-14 | Method: | ELECTRON MICROSCOPY (3.2 Å) | Cite: | Structural basis of RfaH-mediated transcription-translation coupling Nat.Struct.Mol.Biol., 2024
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8U3B
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![BU of 8u3b by Molmil](/molmil-images/mine/8u3b) | Cryo-EM structure of E. coli NarL-transcription activation complex at 3.2A | Descriptor: | DNA (69-MER), DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | Authors: | Liu, B, Kompaniiets, D, Wang, D. | Deposit date: | 2023-09-07 | Release date: | 2024-01-17 | Last modified: | 2024-02-21 | Method: | ELECTRON MICROSCOPY (3.23 Å) | Cite: | Structural basis for transcription activation by the nitrate-responsive regulator NarL. Nucleic Acids Res., 52, 2024
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6N61
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![BU of 6n61 by Molmil](/molmil-images/mine/6n61) | Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA and Capistruin | Descriptor: | 1,2-ETHANEDIOL, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, Capistruin, ... | Authors: | Braffman, N, Hauver, J, Campbell, E.A, Darst, S.A. | Deposit date: | 2018-11-24 | Release date: | 2019-01-09 | Last modified: | 2023-10-11 | Method: | X-RAY DIFFRACTION (3.253 Å) | Cite: | Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin. Proc. Natl. Acad. Sci. U.S.A., 116, 2019
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6OMF
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![BU of 6omf by Molmil](/molmil-images/mine/6omf) | CryoEM structure of SigmaS-transcription initiation complex with activator Crl | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | Authors: | Jaramillo Cartagena, A, Darst, S.A, Campbell, E.A. | Deposit date: | 2019-04-18 | Release date: | 2019-08-28 | Last modified: | 2024-03-13 | Method: | ELECTRON MICROSCOPY (3.26 Å) | Cite: | Structural basis for transcription activation by Crl through tethering of sigmaSand RNA polymerase. Proc.Natl.Acad.Sci.USA, 116, 2019
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8SY6
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![BU of 8sy6 by Molmil](/molmil-images/mine/8sy6) | E. coli DNA-directed RNA polymerase transcription elongation complex bound the unnatural dB-UTP base pair in the active site | Descriptor: | 2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | Authors: | Shan, Z, Lyumkis, D, Oh, J, Wang, D. | Deposit date: | 2023-05-24 | Release date: | 2023-12-27 | Last modified: | 2024-05-29 | Method: | ELECTRON MICROSCOPY (3.28 Å) | Cite: | A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase. Nat Commun, 14, 2023
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6X50
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![BU of 6x50 by Molmil](/molmil-images/mine/6x50) | Mfd-bound E.coli RNA polymerase elongation complex - V state | Descriptor: | ADENOSINE-5'-TRIPHOSPHATE, DNA (64-MER), DNA-directed RNA polymerase subunit alpha, ... | Authors: | Llewelyn, E, Chen, J, Kang, J.Y, Darst, S.A. | Deposit date: | 2020-05-24 | Release date: | 2021-02-03 | Last modified: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (3.3 Å) | Cite: | Structural basis for transcription complex disruption by the Mfd translocase. Elife, 10, 2021
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8EHF
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![BU of 8ehf by Molmil](/molmil-images/mine/8ehf) | Cryo-EM structure of his-elemental paused elongation complex with an unfolded TL (1) | Descriptor: | (3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-dimethyl-17-[(2R)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | Deposit date: | 2022-09-14 | Release date: | 2023-03-01 | Last modified: | 2024-06-19 | Method: | ELECTRON MICROSCOPY (3.3 Å) | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options. Proc.Natl.Acad.Sci.USA, 120, 2023
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8EG8
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![BU of 8eg8 by Molmil](/molmil-images/mine/8eg8) | Cryo-EM structure of consensus elemental paused elongation complex with a folded TL | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | Deposit date: | 2022-09-12 | Release date: | 2023-03-01 | Last modified: | 2024-06-19 | Method: | ELECTRON MICROSCOPY (3.3 Å) | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options. Proc.Natl.Acad.Sci.USA, 120, 2023
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8K5A
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![BU of 8k5a by Molmil](/molmil-images/mine/8k5a) | The cryo-EM map of open TIEA-TIC complex | Descriptor: | 15 kDa RNA polymerase-binding protein, DNA (29-MER), DNA-directed RNA polymerase subunit alpha, ... | Authors: | Zhang, K.N, Liu, Y, Chen, M, Wang, Y, Lin, W, Li, M, Zhang, X, Gao, Y, Gong, Q, Chen, H, Steve, M, Li, S, Zhang, K, Liu, B. | Deposit date: | 2023-07-21 | Release date: | 2024-07-24 | Method: | ELECTRON MICROSCOPY (3.3 Å) | Cite: | TIEA inhibits sigma70-dependent transcriptions, accelerates elongation speed and elevates transcription error To Be Published
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7MKN
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![BU of 7mkn by Molmil](/molmil-images/mine/7mkn) | Escherichia coli RNA polymerase and RapA elongation complex | Descriptor: | 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine, DNA (29-MER), DNA-directed RNA polymerase subunit alpha, ... | Authors: | Qayyum, M.Z, Murakami, K.S. | Deposit date: | 2021-04-26 | Release date: | 2021-06-02 | Last modified: | 2024-05-29 | Method: | ELECTRON MICROSCOPY (3.3 Å) | Cite: | Structural basis of RNA polymerase recycling by the Swi2/Snf2 family of ATPase RapA in Escherichia coli. J.Biol.Chem., 297, 2021
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7UBN
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![BU of 7ubn by Molmil](/molmil-images/mine/7ubn) | |
5UAG
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![BU of 5uag by Molmil](/molmil-images/mine/5uag) | Escherichia coli RNA polymerase mutant - RpoB D516V | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | Authors: | Molodtsov, V, Scharf, N.T, Stefan, M.A, Garcia, G.A, Murakami, K.S. | Deposit date: | 2016-12-19 | Release date: | 2017-02-08 | Last modified: | 2023-10-04 | Method: | X-RAY DIFFRACTION (3.399 Å) | Cite: | Structural basis for rifamycin resistance of bacterial RNA polymerase by the three most clinically important RpoB mutations found in Mycobacterium tuberculosis. Mol. Microbiol., 103, 2017
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8EH8
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![BU of 8eh8 by Molmil](/molmil-images/mine/8eh8) | Cryo-EM structure of his-elemental paused elongation complex with a folded TL and a rotated RH-FL (1) | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | Deposit date: | 2022-09-13 | Release date: | 2023-03-01 | Last modified: | 2024-06-19 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options. Proc.Natl.Acad.Sci.USA, 120, 2023
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8F3C
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![BU of 8f3c by Molmil](/molmil-images/mine/8f3c) | Cryo-EM consensus structure of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase minus preQ1 ligand | Descriptor: | DNA (38-MER), DNA (39-MER), DNA-directed RNA polymerase subunit alpha, ... | Authors: | Porta, J.C, Chauvier, A, Deb, I, Ellinger, E, Frank, A.T, Meze, K, Ohi, M.D, Walter, N.G. | Deposit date: | 2022-11-09 | Release date: | 2023-06-21 | Last modified: | 2024-06-19 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Structural basis for control of bacterial RNA polymerase pausing by a riboswitch and its ligand. Nat.Struct.Mol.Biol., 30, 2023
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6RIP
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![BU of 6rip by Molmil](/molmil-images/mine/6rip) | Cryo-EM structure of E. coli RNA polymerase backtracked elongation complex in swiveled state | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | Authors: | Abdelkareem, M, Saint-Andre, C, Takacs, M, Papai, G, Crucifix, C, Guo, X, Ortiz, J, Weixlbaumer, A. | Deposit date: | 2019-04-24 | Release date: | 2019-07-03 | Last modified: | 2024-05-22 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Structural Basis of Transcription: RNA Polymerase Backtracking and Its Reactivation. Mol.Cell, 75, 2019
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8PFJ
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![BU of 8pfj by Molmil](/molmil-images/mine/8pfj) | fully recruited RfaH bound to E. coli transcription complex paused at ops site (not fully complementary scaffold; alternative state of RfaH) | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | Authors: | Zuber, P.K, Said, N, Hilal, T, Loll, B, Wahl, M.C, Knauer, S.H. | Deposit date: | 2023-06-16 | Release date: | 2024-04-24 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Concerted transformation of a hyper-paused transcription complex and its reinforcing protein. Nat Commun, 15, 2024
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7W5X
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![BU of 7w5x by Molmil](/molmil-images/mine/7w5x) | Cryo-EM structure of SoxS-dependent transcription activation complex with zwf promoter DNA | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | Authors: | Lin, W, Feng, Y, Shi, J. | Deposit date: | 2021-11-30 | Release date: | 2022-10-26 | Last modified: | 2024-06-26 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Structural basis of three different transcription activation strategies adopted by a single regulator SoxS. Nucleic Acids Res., 50, 2022
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7QXI
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6PSR
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![BU of 6psr by Molmil](/molmil-images/mine/6psr) | Escherichia coli RNA polymerase promoter unwinding intermediate (TRPi1) with TraR and rpsT P2 promoter | Descriptor: | CHAPSO, DNA (85-MER), DNA-directed RNA polymerase subunit alpha, ... | Authors: | Chen, J, Chiu, C.E, Campbell, E.A, Darst, S.A. | Deposit date: | 2019-07-13 | Release date: | 2020-03-25 | Last modified: | 2024-03-20 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Stepwise Promoter Melting by Bacterial RNA Polymerase. Mol.Cell, 78, 2020
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7QWP
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![BU of 7qwp by Molmil](/molmil-images/mine/7qwp) | CryoEM structure of bacterial transcription close complex (RPc) | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | Authors: | Ye, F.Z, Zhang, X.D. | Deposit date: | 2022-01-25 | Release date: | 2022-11-09 | Last modified: | 2024-07-17 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Mechanisms of DNA opening revealed in AAA+ transcription complex structures. Sci Adv, 8, 2022
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6ZTJ
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![BU of 6ztj by Molmil](/molmil-images/mine/6ztj) | E. coli 70S-RNAP expressome complex in NusG-coupled state (38 nt intervening mRNA) | Descriptor: | 16S ribosomal RNA, 23S ribosomal RNA, 30S ribosomal protein S1, ... | Authors: | Webster, M.W, Takacs, M, Weixlbaumer, A. | Deposit date: | 2020-07-20 | Release date: | 2020-09-16 | Last modified: | 2024-04-24 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Structural basis of transcription-translation coupling and collision in bacteria. Science, 369, 2020
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8PIM
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![BU of 8pim by Molmil](/molmil-images/mine/8pim) | fully recruited RfaH bound to E. coli transcription complex paused at ops site (not complementary scaffold) | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | Authors: | Zuber, P.K, Said, N, Hilal, T, Loll, B, Wahl, M.C, Knauer, S.H. | Deposit date: | 2023-06-22 | Release date: | 2024-04-24 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Concerted transformation of a hyper-paused transcription complex and its reinforcing protein. Nat Commun, 15, 2024
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8REB
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![BU of 8reb by Molmil](/molmil-images/mine/8reb) | |
8REA
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![BU of 8rea by Molmil](/molmil-images/mine/8rea) | |
6OUL
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![BU of 6oul by Molmil](/molmil-images/mine/6oul) | Cryo-EM structure of Escherichia coli RNAP polymerase bound to rpsTP2 promoter DNA | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | Authors: | Chen, J, Chiu, C.E, Campbell, E.A, Darst, S.A. | Deposit date: | 2019-05-04 | Release date: | 2020-02-26 | Last modified: | 2024-03-20 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | E. coliTraR allosterically regulates transcription initiation by altering RNA polymerase conformation. Elife, 8, 2019
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