8G9U
| Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications | Descriptor: | CRISPR-associated protein, Csd1 family, Csd2 family, ... | Authors: | Hu, C, Nam, K.H, Ke, A. | Deposit date: | 2023-02-22 | Release date: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (3 Å) | Cite: | Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Mol.Cell, 84, 2024
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6C27
| SAM-III riboswitch ON-state | Descriptor: | COBALT HEXAMMINE(III), SAM-III riboswitch | Authors: | Grigg, J.C, Price, I.R, Ke, A. | Deposit date: | 2018-01-07 | Release date: | 2019-01-09 | Last modified: | 2024-05-22 | Method: | X-RAY DIFFRACTION (3.601 Å) | Cite: | Evidence for two-tiered conformation selection in the SAM-III riboswitch To Be Published
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8GAM
| Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications | Descriptor: | Cas11, Cas5, Cas7, ... | Authors: | Hu, C, Nam, K.H, Ke, A. | Deposit date: | 2023-02-23 | Release date: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (3.46 Å) | Cite: | Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Mol.Cell, 84, 2024
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8GAN
| Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications | Descriptor: | Cas11, Cas5, Cas7, ... | Authors: | Hu, C, Nam, K.H, Ke, A. | Deposit date: | 2023-02-23 | Release date: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (3.26 Å) | Cite: | Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Mol.Cell, 84, 2024
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4JRC
| Distal Stem I region from G. kaustophilus glyQS T box RNA | Descriptor: | Distal Stem I region of the glyQS T box leader RNA, MAGNESIUM ION | Authors: | Grigg, J.C, Ke, A. | Deposit date: | 2013-03-21 | Release date: | 2013-04-24 | Last modified: | 2024-02-28 | Method: | X-RAY DIFFRACTION (2.673 Å) | Cite: | T box RNA decodes both the information content and geometry of tRNA to affect gene expression. Proc.Natl.Acad.Sci.USA, 110, 2013
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4MGM
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7UTN
| IscB and wRNA bound to Target DNA | Descriptor: | DNA non-target strand, DNA target strand, IscB, ... | Authors: | Schuler, G.A, Hu, C, Ke, A. | Deposit date: | 2022-04-27 | Release date: | 2022-06-15 | Last modified: | 2024-02-14 | Method: | ELECTRON MICROSCOPY (2.74 Å) | Cite: | Structural basis for RNA-guided DNA cleavage by IscB-omega RNA and mechanistic comparison with Cas9. Science, 376, 2022
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8GAF
| Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications | Descriptor: | Cas11, Cas5, Cas7, ... | Authors: | Hu, C, Nam, K.H, Ke, A. | Deposit date: | 2023-02-22 | Release date: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (3.64 Å) | Cite: | Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Mol.Cell, 84, 2024
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4Y1J
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8G9S
| Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications | Descriptor: | AcrIC8, Cas11, Cas5, ... | Authors: | Hu, C, Nam, K.H, Ke, A. | Deposit date: | 2023-02-22 | Release date: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Mol.Cell, 84, 2024
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8G9T
| Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications | Descriptor: | AcrIC9, Cas11, Cas5, ... | Authors: | Hu, C, Nam, K.H, Ke, A. | Deposit date: | 2023-02-22 | Release date: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (3.6 Å) | Cite: | Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Mol.Cell, 84, 2024
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4Y1I
| Lactococcus lactis yybP-ykoY Mn riboswitch bound to Mn2+ | Descriptor: | BARIUM ION, GUANOSINE-5'-TRIPHOSPHATE, Lactococcus lactis yybP-ykoY riboswitch, ... | Authors: | Price, I.R, Ke, A. | Deposit date: | 2015-02-07 | Release date: | 2015-04-08 | Last modified: | 2024-02-28 | Method: | X-RAY DIFFRACTION (2.85 Å) | Cite: | Mn(2+)-Sensing Mechanisms of yybP-ykoY Orphan Riboswitches. Mol.Cell, 57, 2015
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7UZ0
| AntiT-tRNA flip UCCA | Descriptor: | AntiT-tRNA flip UCCA, MAGNESIUM ION, STRONTIUM ION | Authors: | Price, I.R, Grigg, J.C, Ke, A. | Deposit date: | 2022-05-07 | Release date: | 2022-06-08 | Last modified: | 2023-10-25 | Method: | X-RAY DIFFRACTION (3.03 Å) | Cite: | tRNA Fusion to Streamline RNA Structure Determination: Case Studies in Probing Aminoacyl-tRNA Sensing Mechanisms by the T-Box Riboswitch Crystals, 12, 2022
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6C66
| CRISPR RNA-guided surveillance complex, pre-nicking | Descriptor: | CRISPR-associated helicase, Cas3 family, CRISPR-associated protein, ... | Authors: | Xiao, Y, Luo, M, Liao, M, Ke, A. | Deposit date: | 2018-01-17 | Release date: | 2018-06-27 | Last modified: | 2023-11-29 | Method: | ELECTRON MICROSCOPY (3.66 Å) | Cite: | Structure basis for RNA-guided DNA degradation by Cascade and Cas3. Science, 361, 2018
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5H9E
| Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target (32-nt spacer) at 3.20 angstrom resolution. | Descriptor: | CRISPR system Cascade subunit CasA, CRISPR system Cascade subunit CasB, CRISPR system Cascade subunit CasC, ... | Authors: | Hayes, R.P, Xiao, Y, Ding, F, van Erp, P.B.G, Rajashankar, K, Bailey, S, Wiedenheft, B, Ke, A. | Deposit date: | 2015-12-28 | Release date: | 2016-02-17 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (3.21 Å) | Cite: | Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli. Nature, 530, 2016
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5H9F
| Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target at 2.45 angstrom resolution. | Descriptor: | CRISPR system Cascade subunit CasA, CRISPR system Cascade subunit CasB, CRISPR system Cascade subunit CasC, ... | Authors: | Hayes, R.P, Xiao, Y, Ding, F, van Erp, P.B.G, Rajashankar, K, Bailey, S, Wiedenheft, B, Ke, A. | Deposit date: | 2015-12-28 | Release date: | 2016-02-17 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (2.45 Å) | Cite: | Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli. Nature, 530, 2016
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3L7Z
| Crystal structure of the S. solfataricus archaeal exosome | Descriptor: | Probable exosome complex RNA-binding protein 1, Probable exosome complex exonuclease 1, Probable exosome complex exonuclease 2, ... | Authors: | Lu, C, Ding, F, Ke, A. | Deposit date: | 2009-12-29 | Release date: | 2010-06-23 | Last modified: | 2021-10-13 | Method: | X-RAY DIFFRACTION (2.41 Å) | Cite: | Crystal structure of the S. solfataricus archaeal exosome reveals conformational flexibility in the RNA-binding ring. Plos One, 5, 2010
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7TRA
| Cascade complex from type I-A CRISPR-Cas system | Descriptor: | CRISPR-associated endonuclease Cas3-HD, CRISPR-associated helicase Cas3, Cas11a, ... | Authors: | Hu, C, Ni, D, Nam, K.H, Majumdar, S, McLean, J, Stahlberg, H, Terns, M, Ke, A. | Deposit date: | 2022-01-28 | Release date: | 2022-08-10 | Last modified: | 2022-08-17 | Method: | ELECTRON MICROSCOPY (3.3 Å) | Cite: | Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools. Mol.Cell, 82, 2022
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7TR9
| Cascade complex from type I-A CRISPR-Cas system | Descriptor: | CRISPR-associated endonuclease Cas3-HD, CRISPR-associated helicase Cas3, Cas11a, ... | Authors: | Hu, C, Ni, D, Nam, K.H, Majumdar, S, McLean, J, Stahlberg, H, Terns, M, Ke, A. | Deposit date: | 2022-01-28 | Release date: | 2022-08-10 | Last modified: | 2022-08-24 | Method: | ELECTRON MICROSCOPY (3.9 Å) | Cite: | Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools. Mol.Cell, 82, 2022
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7TR8
| Cascade complex from type I-A CRISPR-Cas system | Descriptor: | CRISPR-associated endonuclease Cas3-HD, CRISPR-associated helicase Cas3, Cas11a, ... | Authors: | Hu, C, Ni, D, Nam, K.H, Majumdar, S, McLean, J, Stahlberg, H, Terns, M, Ke, A. | Deposit date: | 2022-01-28 | Release date: | 2022-08-10 | Last modified: | 2022-08-17 | Method: | ELECTRON MICROSCOPY (3.6 Å) | Cite: | Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools. Mol.Cell, 82, 2022
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5XVN
| E. far Cas1-Cas2/prespacer binary complex | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (28-MER), ... | Authors: | Xiao, Y, Ng, S, Nam, K.H, Ke, A. | Deposit date: | 2017-06-28 | Release date: | 2017-10-04 | Last modified: | 2024-05-22 | Method: | X-RAY DIFFRACTION (3.25 Å) | Cite: | How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration. Nature, 550, 2017
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5XVO
| E. fae Cas1-Cas2/prespacer/target ternary complex revealing DNA sampling and half-integration states | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (28-MER), ... | Authors: | Xiao, Y, Ng, S, Nam, K.H, Ke, A. | Deposit date: | 2017-06-28 | Release date: | 2017-10-04 | Last modified: | 2024-05-22 | Method: | X-RAY DIFFRACTION (3.1 Å) | Cite: | How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration. Nature, 550, 2017
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5XVP
| E. fae Cas1-Cas2/prespacer/target ternary complex revealing the fully integrated states | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (5'-D(P*TP*TP*CP*TP*CP*CP*GP*AP*G)-3'), ... | Authors: | Xiao, Y, Ng, S, Nam, K.H, Ke, A. | Deposit date: | 2017-06-28 | Release date: | 2017-10-04 | Last modified: | 2024-05-22 | Method: | X-RAY DIFFRACTION (3 Å) | Cite: | How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration. Nature, 550, 2017
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7TR6
| Cascade complex from type I-A CRISPR-Cas system | Descriptor: | Cas11a, Cas5a, Cas7a, ... | Authors: | Hu, C, Ni, D, Nam, K.H, Majumdar, S, McLean, J, Stahlberg, H, Terns, M, Ke, A. | Deposit date: | 2022-01-28 | Release date: | 2022-08-10 | Last modified: | 2022-08-17 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools. Mol.Cell, 82, 2022
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6N2V
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