4MGM
Crystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly
Summary for 4MGM
| Entry DOI | 10.2210/pdb4mgm/pdb |
| Related | 4MGN |
| Descriptor | tRNA glycine, MAGNESIUM ION (2 entities in total) |
| Functional Keywords | rna-rna complex, base stacking, t-loop, rna binding, rna |
| Total number of polymer chains | 2 |
| Total formula weight | 48684.94 |
| Authors | Grigg, J.C.,Ke, A. (deposition date: 2013-08-28, release date: 2013-10-23, Last modification date: 2023-09-20) |
| Primary citation | Grigg, J.C.,Ke, A. Structural Determinants for Geometry and Information Decoding of tRNA by T Box Leader RNA. Structure, 21:2025-2032, 2013 Cited by PubMed Abstract: T box riboswitches are cis-acting RNA elements that bind to tRNA and sense its aminoacylation state to influence gene expression. Here, we present the 3.2 Å resolution X-ray crystal structures of the T box Stem I-tRNA complex and tRNA, in isolation. T box Stem I forms an arched conformation with extensive intermolecular contacts to two key points of tRNA, the anticodon and D/T-loops. Free and complexed tRNA exist in significantly different conformations, with the contacts stabilizing flexible D/T-loops and a rearrangement of the D-loop. Using a designed T box RNA/tRNA system, we demonstrate that the T box riboswitch monitors the length and orientation of two essential contacts. Length or orientation mismatches engineered into the T box riboswitch and tRNA disrupt the complex, whereas simultaneous insertion of full helical turns realigns the interfaces and restores interaction between artificially elongated T box riboswitch and tRNA molecules. PubMed: 24095061DOI: 10.1016/j.str.2013.09.001 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
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