4MGM
Crystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 161.233, 28.409, 132.939 |
Unit cell angles | 90.00, 110.69, 90.00 |
Refinement procedure
Resolution | 33.169 - 3.200 |
R-factor | 0.2485 |
Rwork | 0.248 |
R-free | 0.26140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l0u |
RMSD bond length | 0.001 |
RMSD bond angle | 0.403 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 3.260 |
High resolution limit [Å] | 3.200 | 8.640 | 3.200 |
Rmerge | 0.158 | 0.063 | 0.324 |
Number of reflections | 8419 | ||
<I/σ(I)> | 5.5 | 24.7 | 3.14 |
Completeness [%] | 82.8 | 99.8 | 46.2 |
Redundancy | 5.6 | 6.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 75mM NaCl, 2mM CoCl2, 50mM sodium cacodylate, 0.5mM spermine, 30% w/v 1,6-Hexanediol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |