3L0U

The crystal structure of unmodified tRNAPhe from Escherichia coli

Summary for 3L0U

Entry DOI	10.2210/pdb3l0u/pdb
Descriptor	Unmodified tRNAPhe, MAGNESIUM ION, POTASSIUM ION, ... (4 entities in total)
Functional Keywords	transfer rna, trna, transcript, unmodified, rna
Biological source	Escherichia coli K12
Total number of polymer chains	1
Total formula weight	24573.25
Authors	Byrne, R.T.,Konevega, A.L.,Rodnina, M.V.,Antson, A.A. (deposition date: 2009-12-10, release date: 2010-03-16, Last modification date: 2023-11-01)
Primary citation	Byrne, R.T.,Konevega, A.L.,Rodnina, M.V.,Antson, A.A. The crystal structure of unmodified tRNAPhe from Escherichia coli Nucleic Acids Res., 38:4154-4162, 2010 Cited by PubMed Abstract: Post-transcriptional nucleoside modifications fine-tune the biophysical and biochemical properties of transfer RNA (tRNA) so that it is optimized for participation in cellular processes. Here we report the crystal structure of unmodified tRNA(Phe) from Escherichia coli at a resolution of 3 A. We show that in the absence of modifications the overall fold of the tRNA is essentially the same as that of mature tRNA. However, there are a number of significant structural differences, such as rearrangements in a triplet base pair and a widened angle between the acceptor and anticodon stems. Contrary to previous observations, the anticodon adopts the same conformation as seen in mature tRNA. PubMed: 20203084 DOI: 10.1093/nar/gkq133 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3 Å)

Structure validation