1QJO
 
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5DY6
 | | Enhanced superfolder GFP with DBCO at 148 | | Descriptor: | Green fluorescent protein, SULFATE ION | | Authors: | Jones, D.D, Rizkallah, P.J, Worthy, H.L. | | Deposit date: | 2015-09-24 | | Release date: | 2016-07-13 | | Last modified: | 2024-10-16 | | Method: | X-RAY DIFFRACTION (2.66 Å) | | Cite: | Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle.
Chem Sci, 7, 2016
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4EUL
 | | Crystal structure of enhanced Green Fluorescent Protein to 1.35A resolution reveals alternative conformations for Glu222 | | Descriptor: | CALCIUM ION, DI(HYDROXYETHYL)ETHER, Green fluorescent protein, ... | | Authors: | Jones, D.D, Arpino, J.A.J, Rizkallah, P.J. | | Deposit date: | 2012-04-25 | | Release date: | 2012-10-03 | | Last modified: | 2024-11-27 | | Method: | X-RAY DIFFRACTION (1.35 Å) | | Cite: | Crystal structure of enhanced green fluorescent protein to 1.35 a resolution reveals alternative conformations for glu222.
Plos One, 7, 2012
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9F03
 | | mRhubarb720-WT | | Descriptor: | 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid, mRhubarb720 fluorescent protein | | Authors: | Aksakal, O, Rizkallah, P.J, Warren, A, Lipka-Lloyd, M, Jones, D.D. | | Deposit date: | 2024-04-15 | | Release date: | 2025-10-29 | | Last modified: | 2025-11-19 | | Method: | X-RAY DIFFRACTION (3.3 Å) | | Cite: | Monomeric rhubarb fluorescent protein
To Be Published
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8C1X
 | | sfGFP C148 F206 mutant | | Descriptor: | Green fluorescent protein | | Authors: | Ahmed, R.D, Rizkallah, P.J, Jones, D.D. | | Deposit date: | 2022-12-21 | | Release date: | 2024-01-10 | | Last modified: | 2025-11-12 | | Method: | X-RAY DIFFRACTION (1.89 Å) | | Cite: | Chromophore charge-state switching through copper-dependent homodimerisation of an engineered green fluorescent protein.
Chem Sci, 2025
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7B4Q
 | | Structure of a cold active HSL family esterase reveals mechanisms of low temperature adaptation and substrate specificity | | Descriptor: | 1,2-ETHANEDIOL, DI(HYDROXYETHYL)ETHER, GLYCEROL, ... | | Authors: | Rizkallah, P.J, Jones, D.D, Noby, N, Auhim, H. | | Deposit date: | 2020-12-02 | | Release date: | 2021-12-15 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (1.61 Å) | | Cite: | Structure and in silico simulations of a cold-active esterase reveals its prime cold-adaptation mechanism.
Open Biology, 11, 2021
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8BXP
 | | SfGFP C148 F206 mutant | | Descriptor: | Green fluorescent protein | | Authors: | Ahmed, R.D, Rizkallah, P.J, Jones, D.D. | | Deposit date: | 2022-12-09 | | Release date: | 2023-12-20 | | Last modified: | 2025-11-12 | | Method: | X-RAY DIFFRACTION (1.79 Å) | | Cite: | Chromophore charge-state switching through copper-dependent homodimerisation of an engineered green fluorescent protein.
Chem Sci, 2025
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3U8P
 | | Cytochrome b562 integral fusion with EGFP | | Descriptor: | Cytochrome b562 integral fusion with enhanced green fluorescent protein, PROTOPORPHYRIN IX CONTAINING FE | | Authors: | Arpino, J, Czapinska, H, Piasecka, A, Edwards, W.R, Barker, P, Gajda, M, Bochtler, M, Jones, D.D. | | Deposit date: | 2011-10-17 | | Release date: | 2012-08-29 | | Last modified: | 2024-11-27 | | Method: | X-RAY DIFFRACTION (2.75 Å) | | Cite: | Structural basis for efficient chromophore communication and energy transfer in a constructed didomain protein scaffold.
J.Am.Chem.Soc., 134, 2012
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4ZIO
 | | Irradiated state of mCherry143azF | | Descriptor: | SULFATE ION, mCherry | | Authors: | Reddington, S.C, Driezis, S, Hartley, A.M, Watson, P.D, Rizkallah, P.J, Jones, D.D. | | Deposit date: | 2015-04-28 | | Release date: | 2015-09-16 | | Last modified: | 2025-04-09 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein
Rsc Adv, 5, 2015
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6SM0
 | | Venus 66 p-Azido-L-Phenylalanin (azF) variant, dark grown | | Descriptor: | Green fluorescent protein, OXYGEN MOLECULE, SULFATE ION, ... | | Authors: | Rizkallah, P.J, Al Maslookhi, H.S, Jones, D.D. | | Deposit date: | 2019-08-21 | | Release date: | 2021-02-17 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (1.91 Å) | | Cite: | Stalling chromophore synthesis of the fluorescent protein Venus reveals the molecular basis of the final oxidation step.
Chem Sci, 12, 2021
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2K7V
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4J89
 | | Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence | | Descriptor: | 1,2-ETHANEDIOL, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, Green fluorescent protein, ... | | Authors: | Reddington, S.C, Jones, D.D, Rizkallah, P.J, Tippmann, E.M. | | Deposit date: | 2013-02-14 | | Release date: | 2013-06-26 | | Last modified: | 2023-12-06 | | Method: | X-RAY DIFFRACTION (2.1 Å) | | Cite: | Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence.
Angew.Chem.Int.Ed.Engl., 52, 2013
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4J8A
 | | Irradiated-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 145 | | Descriptor: | 1,2-ETHANEDIOL, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, Green fluorescent protein, ... | | Authors: | Reddington, S.C, Jones, D.D, Rizkallah, P.J, Tippmann, E.M. | | Deposit date: | 2013-02-14 | | Release date: | 2013-05-15 | | Last modified: | 2023-12-06 | | Method: | X-RAY DIFFRACTION (1.26 Å) | | Cite: | Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence.
Angew.Chem.Int.Ed.Engl., 52, 2013
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4J88
 | | Dark-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 66 | | Descriptor: | 1,2-ETHANEDIOL, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, Green fluorescent protein, ... | | Authors: | Reddington, S.C, Jones, D.D, Rizkallah, P.J, Tippmann, E.M. | | Deposit date: | 2013-02-14 | | Release date: | 2013-06-26 | | Last modified: | 2023-11-15 | | Method: | X-RAY DIFFRACTION (2.08 Å) | | Cite: | Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence.
Angew.Chem.Int.Ed.Engl., 52, 2013
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2WWT
 | | Intracellular subtilisin precursor from B. clausii | | Descriptor: | INTRACELLULAR SUBTILISIN PROTEASE, SODIUM ION | | Authors: | Vevodova, J, Gamble, M, Ariza, A, Dodson, E, Jones, D.D, Wilson, K.S. | | Deposit date: | 2009-10-27 | | Release date: | 2010-09-08 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (2.68 Å) | | Cite: | Crystal Structure of an Intracellular Subtilisin Reveals Novel Structural Features Unique to This Subtilisin Family.
Structure, 18, 2010
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2WV7
 | | Intracellular subtilisin precursor from B. clausii | | Descriptor: | INTRACELLULAR SUBTILISIN PROTEASE, SODIUM ION | | Authors: | Vevodova, J, Gamble, M, Ariza, A, Dodson, E, Jones, D.D, Wilson, K.S. | | Deposit date: | 2009-10-15 | | Release date: | 2010-09-08 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (2.45 Å) | | Cite: | Crystal Structure of an Intracellular Subtilisin Reveals Novel Structural Features Unique to This Subtilisin Family.
Structure, 18, 2010
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2XRM
 | | Processed Intracellular subtilisin from B. clausii | | Descriptor: | CALCIUM ION, INTRACELLULAR SUBTILISIN PROTEASE, PENTAETHYLENE GLYCOL, ... | | Authors: | Gamble, M, Kunze, G, Dodson, E.J, Jones, D.D, Wilson, K.S. | | Deposit date: | 2010-09-20 | | Release date: | 2011-03-16 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (2.6 Å) | | Cite: | Regulation of an Intracellular Subtilisin Protease Activity by a Short Propeptide Sequence Through an Original Combined Dual Mechanism.
Proc.Natl.Acad.Sci.USA, 108, 2011
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2X8J
 | | Intracellular subtilisin precursor from B. clausii | | Descriptor: | GLYCEROL, INTRACELLULAR SUBTILISIN PROTEASE, PENTAETHYLENE GLYCOL, ... | | Authors: | Vedodova, J, Gamble, M, Ariza, A, Dodson, E, Jones, D.D, Wilson, K.S. | | Deposit date: | 2010-03-09 | | Release date: | 2010-08-11 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (1.56 Å) | | Cite: | Crystal structure of an intracellular subtilisin reveals novel structural features unique to this subtilisin family.
Structure, 18, 2010
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4ZIN
 | | Genetically encoded Phenyl Azide Photochemistry Drive Positive and Negative Functional Modulation of a Red Fluorescent Protein | | Descriptor: | 1,2-ETHANEDIOL, MCherry fluorescent protein, SULFATE ION | | Authors: | Reddington, S.C, Driezis, S, Hartley, A.M, Watson, P.D, Rizkallah, P.J, Jones, D.D. | | Deposit date: | 2015-04-28 | | Release date: | 2015-09-16 | | Last modified: | 2024-05-29 | | Method: | X-RAY DIFFRACTION (1.67 Å) | | Cite: | Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein
Rsc Adv, 5, 2015
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5BTT
 | | Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position. | | Descriptor: | GLYCEROL, Green fluorescent protein, SULFATE ION | | Authors: | Hartley, A.M, Worthy, H.L, Reddington, S.C, Rizkallah, P.J, Jones, D.D. | | Deposit date: | 2015-06-03 | | Release date: | 2016-07-13 | | Last modified: | 2025-04-09 | | Method: | X-RAY DIFFRACTION (2.14 Å) | | Cite: | Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle.
Chem Sci, 7, 2016
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5BT0
 | | Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position. | | Descriptor: | Green fluorescent protein, SULFATE ION | | Authors: | Hartley, A.M, Worthy, H.L, Reddington, S.C, Rizkallah, P.J, Jones, D.D. | | Deposit date: | 2015-06-02 | | Release date: | 2016-07-13 | | Last modified: | 2025-04-09 | | Method: | X-RAY DIFFRACTION (2.03 Å) | | Cite: | Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle.
Chem Sci, 7, 2016
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5NHN
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5NI3
 | | sfGFP 204-204 mutant dimer | | Descriptor: | CHLORIDE ION, GLYCEROL, Green fluorescent protein, ... | | Authors: | Worthy, H.L, Rizkallah, P.J. | | Deposit date: | 2017-03-23 | | Release date: | 2018-09-12 | | Last modified: | 2024-11-20 | | Method: | X-RAY DIFFRACTION (1.28 Å) | | Cite: | Association of Fluorescent Protein Pairs and Its Significant Impact on Fluorescence and Energy Transfer
Adv Sci, 2020
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4KAG
 | | Crystal structure analysis of a single amino acid deletion mutation in EGFP | | Descriptor: | 1,2-ETHANEDIOL, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, Green fluorescent protein, ... | | Authors: | Arpino, J.A.J, Rizkallah, P.J. | | Deposit date: | 2013-04-22 | | Release date: | 2014-08-06 | | Last modified: | 2024-10-16 | | Method: | X-RAY DIFFRACTION (1.12 Å) | | Cite: | Structural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent protein.
Acta Crystallogr.,Sect.D, 70, 2014
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4KA9
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