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5BT0

Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position.

Summary for 5BT0
Entry DOI10.2210/pdb5bt0/pdb
DescriptorGreen fluorescent protein, SULFATE ION (3 entities in total)
Functional Keywordssynthetic biology, photocontrol, optogenetics, unnatural amino acids, protein fluorescence, sfgfp, fluorescent protein
Biological sourceAequorea victoria (Jellyfish)
Total number of polymer chains2
Total formula weight52891.21
Authors
Hartley, A.M.,Worthy, H.L.,Reddington, S.C.,Rizkallah, P.J.,Jones, D.D. (deposition date: 2015-06-02, release date: 2016-07-13, Last modification date: 2017-05-10)
Primary citationHartley, A.M.,Worthy, H.L.,Reddington, S.C.,Rizkallah, P.J.,Jones, D.D.
Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle.
Chem Sci, 7:6484-6491, 2016
Cited by
PubMed: 28451106
DOI: 10.1039/c6sc00944a
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.03 Å)
Structure validation

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