5BT0
Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Green fluorescent protein | polymer | 233 | 26157.4 | 2 | UniProt (A0A059PIQ0) Pfam (PF01353) | Aequorea victoria (Jellyfish) | |
| 2 | C, D, E, F, G... (A, B) | SULFATE ION | non-polymer | 96.1 | 6 | Chemie (SO4) | |||
| 3 | I, J (A, B) | water | water | 18.0 | 345 | Chemie (HOH) |
Sequence modifications
A, B: 3 - 234 (UniProt: A0A059PIQ0)
| PDB | External Database | Details |
|---|---|---|
| Ser 2 | - | expression tag |
| Arg 30 | Ser 30 | conflict |
| Cro | Thr 65 | chromophore |
| Cro | Tyr 66 | chromophore |
| Cro 66 | Gly 67 | chromophore |
| Ser 72 | Ala 72 | conflict |
| Arg 80 | Gln 80 | conflict |
| Hox 148 | His 148 | conflict |
| Val 206 | Ala 206 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 52314.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 576.4 | |
| All* | Total formula weight | 52891.2 |
