4J89

Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence

Summary for 4J89

• Entry DOI: 10.2210/pdb4j89/pdb
• Related: 4J88 4J8A
• Descriptor: Green fluorescent protein, SULFATE ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• Functional Keywords: beta-barrel, fluorescent protein, chromophore by cyclisation, p-azido-l-phenylalanine, cytosol
• Biological source: Aequorea victoria (Jellyfish)
• Total number of polymer chains: 2
• Total formula weight: 57770.73

Authors

Reddington, S.C.,Jones, D.D.,Rizkallah, P.J.,Tippmann, E.M. (deposition date: 2013-02-14, release date: 2013-06-26, Last modification date: 2023-12-06)

Primary citation

Reddington, S.C.,Rizkallah, P.J.,Watson, P.D.,Pearson, R.,Tippmann, E.M.,Jones, D.D.
Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence.
Angew.Chem.Int.Ed.Engl., 52:5974-5977, 2013

PubMed Abstract: Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.

PubMed: 23620472
DOI: 10.1002/anie.201301490

Experimental method

X-RAY DIFFRACTION (2.1 Å)