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8W2Q
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BU of 8w2q by Molmil
BsaXI-DNA complex II
Descriptor: DNA (41-MER), RM.BsaXI, S-ADENOSYL-L-HOMOCYSTEINE, ...
Authors:Shen, B.W, Stoddard, B.L, Xu, S.
Deposit date:2024-02-21
Release date:2024-10-30
Method:ELECTRON MICROSCOPY (3.06 Å)
Cite:Structural and biochemical analysis of subunit assembly, DNA recognition and cleavage by a Type IIB restriction-modification enzyme: BsaXI
To Be Published
8W2P
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BU of 8w2p by Molmil
BsaXI-DNA complex I
Descriptor: CALCIUM ION, DNA (41-MER) Complementary strand of BsaXI DNA substrate, DNA (41-MER) Top strand of BsaXI cognate DNA substrate, ...
Authors:Shen, B.W, Stoddard, B.L, Xu, S.
Deposit date:2024-02-20
Release date:2024-10-30
Method:ELECTRON MICROSCOPY (3.14 Å)
Cite:Structural and biochemical analysis of subunit assembly, DNA recognition and cleavage by a Type IIB restriction-modification enzyme: BsaXI
To Be Published
1EVX
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BU of 1evx by Molmil
APO CRYSTAL STRUCTURE OF THE HOMING ENDONUCLEASE, I-PPOI
Descriptor: INTRON-ENCODED HOMING ENDONUCLEASE I-PPOI, SULFATE ION, ZINC ION
Authors:Galburt, E.A, Jurica, M.S, Chevalier, B.S, Erho, D, Stoddard, B.L.
Deposit date:2000-04-20
Release date:2000-08-02
Last modified:2024-02-07
Method:X-RAY DIFFRACTION (2 Å)
Cite:Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site.
J.Mol.Biol., 300, 2000
1AF5
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BU of 1af5 by Molmil
GROUP I MOBILE INTRON ENDONUCLEASE
Descriptor: I-CREI
Authors:Heath, P.J, Stephens, K.M, Monnat Junior, R.J, Stoddard, B.L.
Deposit date:1997-03-21
Release date:1997-07-23
Last modified:2024-02-07
Method:X-RAY DIFFRACTION (3 Å)
Cite:The structure of I-Crel, a group I intron-encoded homing endonuclease.
Nat.Struct.Biol., 4, 1997
1DT0
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BU of 1dt0 by Molmil
CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS
Descriptor: FE (III) ION, SUPEROXIDE DISMUTASE
Authors:Bond, C.J, Huang, J, Hajduk, R, Flick, K, Heath, P, Stoddard, B.L.
Deposit date:2000-01-10
Release date:2000-12-20
Last modified:2024-02-07
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis.
Acta Crystallogr.,Sect.D, 56, 2000
5TH3
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BU of 5th3 by Molmil
Restriction/modification system-Type II R.SwaI cleaved DNA complex
Descriptor: 1,2-ETHANEDIOL, ACETATE ION, DNA (cleaved 25-MER, ...
Authors:Shen, B.W, Stoddard, B.L.
Deposit date:2016-09-29
Release date:2016-12-21
Last modified:2024-10-30
Method:X-RAY DIFFRACTION (2.33 Å)
Cite:DNA recognition by the SwaI restriction endonuclease involves unusual distortion of an 8 base pair A:T-rich target.
Nucleic Acids Res., 45, 2017
5THG
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BU of 5thg by Molmil
Engineered variant of I-OnuI meganuclease targeting the HIV CCR5 gene; harbors 43 point mutations relative to wild-type I-OnuI
Descriptor: CALCIUM ION, DNA (29-MER), GLYCEROL, ...
Authors:Hallinan, J.P, Stoddard, B.L.
Deposit date:2016-09-29
Release date:2017-05-03
Last modified:2024-03-06
Method:X-RAY DIFFRACTION (3.106 Å)
Cite:The structural basis of altered gene specificity resulting from meganuclease and MegaTAL engineering
to be published
1CYQ
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BU of 1cyq by Molmil
INTRON ENCODED HOMING ENDONUCLEASE I-PPOI (H98A)/DNA HOMING SITE COMPLEX
Descriptor: 5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3', INTRON-ENCODED HOMING ENDONUCLEASE I-PPOI, MAGNESIUM ION, ...
Authors:Galburt, E.A, Chevalier, B, Jurica, M.S, Flick, K.E, Stoddard, B.L.
Deposit date:1999-08-31
Release date:1999-11-19
Last modified:2024-02-07
Method:X-RAY DIFFRACTION (1.93 Å)
Cite:A novel endonuclease mechanism directly visualized for I-PpoI.
Nat.Struct.Biol., 6, 1999
1BP7
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BU of 1bp7 by Molmil
GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA
Descriptor: CALCIUM ION, DNA (5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP* GP*C)-3'), DNA (5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP* CP*G)-3'), ...
Authors:Jurica, M.S, Monnat Junior, R.J, Stoddard, B.L.
Deposit date:1998-08-13
Release date:1999-01-06
Last modified:2023-08-02
Method:X-RAY DIFFRACTION (3 Å)
Cite:DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI.
Mol.Cell, 2, 1998
1D7P
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BU of 1d7p by Molmil
Crystal structure of the c2 domain of human factor viii at 1.5 a resolution at 1.5 A
Descriptor: COAGULATION FACTOR VIII PRECURSOR, CYSTEINE, GLYCEROL, ...
Authors:Pratt, K.P, Shen, B.W, Stoddard, B.L.
Deposit date:1999-10-19
Release date:1999-12-01
Last modified:2024-10-30
Method:X-RAY DIFFRACTION (1.5 Å)
Cite:Structure of the C2 domain of human factor VIII at 1.5 A resolution.
Nature, 402, 1999
1G9Y
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BU of 1g9y by Molmil
HOMING ENDONUCLEASE I-CREI / DNA SUBSTRATE COMPLEX WITH CALCIUM
Descriptor: 5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP*GP*C)-3', 5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP*CP*G)-3', CALCIUM ION, ...
Authors:Chevalier, B, Monnat, R.J, Stoddard, B.L.
Deposit date:2000-11-28
Release date:2001-04-02
Last modified:2023-08-09
Method:X-RAY DIFFRACTION (2.05 Å)
Cite:The homing endonuclease I-CreI uses three metals, one of which is shared between the two active sites.
Nat.Struct.Biol., 8, 2001
1A3W
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BU of 1a3w by Molmil
PYRUVATE KINASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH FBP, PG, MN2+ AND K+
Descriptor: 1,6-di-O-phosphono-beta-D-fructofuranose, 2-PHOSPHOGLYCOLIC ACID, MANGANESE (II) ION, ...
Authors:Jurica, M.S, Mesecar, A, Heath, P.J, Shi, W, Nowak, T, Stoddard, B.L.
Deposit date:1998-01-26
Release date:1998-05-27
Last modified:2024-05-22
Method:X-RAY DIFFRACTION (3 Å)
Cite:The allosteric regulation of pyruvate kinase by fructose-1,6-bisphosphate.
Structure, 6, 1998
1B0A
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BU of 1b0a by Molmil
5,10, METHYLENE-TETRAHYDROPHOLATE DEHYDROGENASE/CYCLOHYDROLASE FROM E COLI.
Descriptor: PROTEIN (FOLD BIFUNCTIONAL PROTEIN)
Authors:Shen, B.W, Dyer, D, Huang, J.-Y, D'Ari, L, Rabinowitz, J, Stoddard, B.L.
Deposit date:1998-11-06
Release date:1999-06-29
Last modified:2023-12-27
Method:X-RAY DIFFRACTION (2.56 Å)
Cite:The crystal structure of a bacterial, bifunctional 5,10 methylene-tetrahydrofolate dehydrogenase/cyclohydrolase.
Protein Sci., 8, 1999
1G9Z
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BU of 1g9z by Molmil
LAGLIDADG HOMING ENDONUCLEASE I-CREI / DNA PRODUCT COMPLEX WITH MAGNESIUM
Descriptor: 5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*C)-3', 5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*A)-3', 5'-D(P*GP*AP*CP*AP*GP*TP*TP*TP*CP*G)-3', ...
Authors:Chevalier, B, Monnat, R.J, Stoddard, B.L.
Deposit date:2000-11-28
Release date:2001-04-02
Last modified:2023-08-09
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:The homing endonuclease I-CreI uses three metals, one of which is shared between the two active sites.
Nat.Struct.Biol., 8, 2001
1A3X
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BU of 1a3x by Molmil
PYRUVATE KINASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH PG, MN2+ AND K+
Descriptor: 2-PHOSPHOGLYCOLIC ACID, MANGANESE (II) ION, POTASSIUM ION, ...
Authors:Jurica, M.S, Mesecar, A, Heath, P.J, Shi, W, Nowak, T, Stoddard, B.L.
Deposit date:1998-01-26
Release date:1998-05-27
Last modified:2024-07-10
Method:X-RAY DIFFRACTION (3 Å)
Cite:The allosteric regulation of pyruvate kinase by fructose-1,6-bisphosphate.
Structure, 6, 1998
8E0N
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BU of 8e0n by Molmil
Homotrimeric variant of tcTRP9, BGL18
Descriptor: BGL18
Authors:Kibler, R, Stoddard, B.L, Kennedy, M.A.
Deposit date:2022-08-09
Release date:2023-04-12
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (3 Å)
Cite:Homotrimeric variant of tcTRP9, BGL18
To Be Published
8E0M
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BU of 8e0m by Molmil
Homotrimeric variant of tcTRP9, BGL15
Descriptor: BGL15
Authors:Kibler, R, Stoddard, B.L, Kennedy, M.A.
Deposit date:2022-08-09
Release date:2023-04-12
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (4 Å)
Cite:Homotrimeric variant of tcTRP9, BGL15
To Be Published
8E0O
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BU of 8e0o by Molmil
Heterotrimeric variant of tcTRP9, hetBGL03-15-18
Descriptor: hetBGL03-15-18a, hetBGL03-15-18b, hetBGL03-15-18c
Authors:Kibler, R, Stoddard, B.L, Kennedy, M.A.
Deposit date:2022-08-09
Release date:2023-04-12
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Heterotrimeric variant of tcTRP9, hetBGL03-15-18
To Be Published
8E12
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BU of 8e12 by Molmil
Homotrimeric variant of tcTRP9, BGL14
Descriptor: BGL14
Authors:Kibler, R, Stoddard, B.L, Kennedy, M.A.
Deposit date:2022-08-09
Release date:2023-04-12
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (3 Å)
Cite:Homotrimeric variant of tcTRP9, BGL14
To Be Published
8E0L
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BU of 8e0l by Molmil
Homotrimeric variant of tcTRP9, BGL06
Descriptor: BGL06
Authors:Kennedy, M.A, Kibler, R, Stoddard, B.L.
Deposit date:2022-08-09
Release date:2023-04-12
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Homotrimeric variant of tcTRP9, BGL06
To Be Published
7RCF
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BU of 7rcf by Molmil
Fourth stage reengineered variant of I-OnuI with stability enhancing substitutions
Descriptor: 1,2-ETHANEDIOL, CALCIUM ION, CHLORIDE ION, ...
Authors:Ubilla-Rodriguez, N.C, Werther, R.A, Stoddard, B.L.
Deposit date:2021-07-07
Release date:2022-06-29
Last modified:2023-10-18
Method:X-RAY DIFFRACTION (2.233 Å)
Cite:Characterization of the stepwise engineering and optimization of a retargeted DNA binding protein and gene-editing meganuclease
To Be Published
7RCG
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BU of 7rcg by Molmil
I-OnuI_e-hPD1-f final stage reengineered variant of I-OnuI
Descriptor: CALCIUM ION, CHLORIDE ION, DNA (26-MER), ...
Authors:Smiley, A.T, Werther, R.A, Stoddard, B.L.
Deposit date:2021-07-07
Release date:2022-06-29
Last modified:2023-10-18
Method:X-RAY DIFFRACTION (2.368 Å)
Cite:Characterization of the stepwise engineering and optimization of a retargeted DNA binding protein and gene-editing meganuclease
To Be Published
7RCD
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BU of 7rcd by Molmil
Second stage reengineered variant of I-OnuI targeting human PD1 gene with activity enhancing substitutions
Descriptor: CALCIUM ION, DNA (26-MER), I-OnuI_e-hPD1-c
Authors:Ubilla-Rodriguez, N.C, Werther, R.A, Stoddard, B.L.
Deposit date:2021-07-07
Release date:2022-06-29
Last modified:2023-10-18
Method:X-RAY DIFFRACTION (2.448 Å)
Cite:Characterization of the stepwise engineering and optimization of a retargeted DNA binding protein and gene-editing meganuclease
To Be Published
7RCE
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BU of 7rce by Molmil
Third stage reengineered variant of I-OnuI with specificity enhancing substitutions
Descriptor: CALCIUM ION, DNA (26-MER), I-OnuI_e-hPD1-d, ...
Authors:Ubilla-Rodriguez, N.C, Werther, R.A, Stoddard, B.L.
Deposit date:2021-07-07
Release date:2022-06-29
Last modified:2023-10-18
Method:X-RAY DIFFRACTION (2.42 Å)
Cite:Characterization of the stepwise engineering and optimization of a retargeted DNA binding protein and gene-editing meganuclease
To Be Published
7RCC
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BU of 7rcc by Molmil
First stage engineered variant of I-OnuI after initial reassembly
Descriptor: CALCIUM ION, DNA (26-MER), I-OnuI_e-hPD1-b
Authors:Ubilla-Rodriguez, N.C, Werther, R.A, Stoddard, B.L.
Deposit date:2021-07-07
Release date:2022-06-29
Last modified:2023-10-18
Method:X-RAY DIFFRACTION (2.45 Å)
Cite:Characterization of the stepwise engineering and optimization of a retargeted DNA binding protein and gene-editing meganuclease
To Be Published

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