5THG
Engineered variant of I-OnuI meganuclease targeting the HIV CCR5 gene; harbors 43 point mutations relative to wild-type I-OnuI
Summary for 5THG
Entry DOI | 10.2210/pdb5thg/pdb |
Descriptor | I-OnuI_e-hCCR5, DNA (29-MER), CALCIUM ION, ... (6 entities in total) |
Functional Keywords | meganuclease, engineered protein, dna complex, hydrolase-dna complex, hydrolase/dna |
Biological source | synthetic construct More |
Total number of polymer chains | 6 |
Total formula weight | 106294.69 |
Authors | Hallinan, J.P.,Stoddard, B.L. (deposition date: 2016-09-29, release date: 2017-05-03, Last modification date: 2024-03-06) |
Primary citation | Werther, R.,Hallinan, J.P.,Lambert, A.,Haven, K.,Jarjor, J.,Stoddard, B.L. The structural basis of altered gene specificity resulting from meganuclease and MegaTAL engineering to be published, |
Experimental method | X-RAY DIFFRACTION (3.106 Å) |
Structure validation
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