5THG

Engineered variant of I-OnuI meganuclease targeting the HIV CCR5 gene; harbors 43 point mutations relative to wild-type I-OnuI

Summary for 5THG

• Entry DOI: 10.2210/pdb5thg/pdb
• Descriptor: I-OnuI_e-hCCR5, DNA (29-MER), CALCIUM ION, ... (6 entities in total)
• Functional Keywords: meganuclease, engineered protein, dna complex, hydrolase-dna complex, hydrolase/dna
• Biological source: synthetic construct; More
• Total number of polymer chains: 6
• Total formula weight: 106294.69

Authors

Hallinan, J.P.,Stoddard, B.L. (deposition date: 2016-09-29, release date: 2017-05-03, Last modification date: 2024-03-06)

• Primary citation: Werther, R.,Hallinan, J.P.,Lambert, A.,Haven, K.,Jarjor, J.,Stoddard, B.L.; The structural basis of altered gene specificity resulting from meganuclease and MegaTAL engineering; to be published,

Experimental method

X-RAY DIFFRACTION (3.106 Å)