7RCF

Fourth stage reengineered variant of I-OnuI with stability enhancing substitutions

Summary for 7RCF

• Entry DOI: 10.2210/pdb7rcf/pdb
• Descriptor: I-OnuI_e-hPD1-e, DNA (26-MER), 1,2-ETHANEDIOL, ... (8 entities in total)
• Functional Keywords: meganuclease, gene editing, protein engineering, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Synthetic construct; More
• Total number of polymer chains: 3
• Total formula weight: 51372.55

Authors

Ubilla-Rodriguez, N.C.,Werther, R.A.,Stoddard, B.L. (deposition date: 2021-07-07, release date: 2022-06-29, Last modification date: 2023-10-18)

• Primary citation: Werther, R.A.,Ubilla-Rodriguez, N.C.,Smiley, A.T.,Havens, K.,Lambert, A.R.,Stoddard, B.L.; Characterization of the stepwise engineering and optimization of a retargeted DNA binding protein and gene-editing meganuclease; To Be Published,

Experimental method

X-RAY DIFFRACTION (2.233 Å)