1DT0

CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS

Summary for 1DT0

• Entry DOI: 10.2210/pdb1dt0/pdb
• Related: 1ar4 1ids 1isb 1isc
• Descriptor: SUPEROXIDE DISMUTASE, FE (III) ION (3 entities in total)
• Functional Keywords: superoxide dismutase, pseudomonas ovalis, oxidoreductase
• Biological source: Pseudomonas putida
• Total number of polymer chains: 3
• Total formula weight: 65901.92

Authors

Bond, C.J.,Huang, J.,Hajduk, R.,Flick, K.,Heath, P.,Stoddard, B.L. (deposition date: 2000-01-10, release date: 2000-12-20, Last modification date: 2024-02-07)

Primary citation

Bond, C.J.,Huang, J.,Hajduk, R.,Flick, K.E.,Heath, P.J.,Stoddard, B.L.
Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis.
Acta Crystallogr.,Sect.D, 56:1359-1366, 2000

PubMed Abstract: The gene encoding the iron-dependent superoxide dismutase from Pseudomonas ovalis was cloned from a genomic library and sequenced. The ORF differs from the previously published protein sequence, which was used for the original structure determination, at 16 positions. The differences include three additional inserted residues, one deleted residue and 12 point substitutions. The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution.

PubMed: 11053832
DOI: 10.1107/S0907444900009537

Experimental method

X-RAY DIFFRACTION (2.1 Å)