1DT0

CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	1998-04-28
Detector	RIGAKU RAXIS II
Spacegroup name	P 32 2 1
Unit cell lengths	115.030, 115.030, 82.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.22
Rwork	0.220
R-free	0.29000
RMSD bond length	0.009 *
RMSD bond angle	1.300 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.048	0.230
Number of reflections	36255
<I/σ(I)>	14.4
Completeness [%]	97.6	93.7
Redundancy	6.3	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	2.6M Ammonium Sulfate 100mM Tris, pH 8.0, vapor diffusion, hanging drop, temperature 25K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	drop	Tris-HCl	100 (mM)
3	1	reservoir	ammonium sulfate	2.0-2.6 (M)
4	1	reservoir	Tris-HCl	100 (mM)