7RCE
Third stage reengineered variant of I-OnuI with specificity enhancing substitutions
Summary for 7RCE
Entry DOI | 10.2210/pdb7rce/pdb |
Descriptor | I-OnuI_e-hPD1-d, DNA (26-MER), CALCIUM ION, ... (6 entities in total) |
Functional Keywords | meganuclease, gene editing, protein engineering, dna binding protein-dna complex, dna binding protein/dna |
Biological source | Synthetic construct More |
Total number of polymer chains | 3 |
Total formula weight | 51107.81 |
Authors | Ubilla-Rodriguez, N.C.,Werther, R.A.,Stoddard, B.L. (deposition date: 2021-07-07, release date: 2022-06-29, Last modification date: 2023-10-18) |
Primary citation | Werther, R.A.,Ubilla-Rodriguez, N.C.,Smiley, A.T.,Havens, K.,Lambert, A.R.,Stoddard, B.L. Characterization of the stepwise engineering and optimization of a retargeted DNA binding protein and gene-editing meganuclease To Be Published, |
Experimental method | X-RAY DIFFRACTION (2.42 Å) |
Structure validation
Download full validation report