1BP7

GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA

Summary for 1BP7

• Entry DOI: 10.2210/pdb1bp7/pdb
• Descriptor: DNA (5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP* CP*G)-3'), DNA (5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP* GP*C)-3'), PROTEIN (I-CREI), ... (5 entities in total)
• Functional Keywords: endonuclease, group i mobile intron, intron homing, chloroplast dna, laglidadg motif, dna complex, transcription-dna complex, transcription/dna
• Biological source: Chlamydomonas reinhardtii
• Cellular location: Plastid, chloroplast: P05725
• Total number of polymer chains: 8
• Total formula weight: 99703.86

Authors

Jurica, M.S.,Monnat Junior, R.J.,Stoddard, B.L. (deposition date: 1998-08-13, release date: 1999-01-06, Last modification date: 2023-08-02)

Primary citation

Jurica, M.S.,Monnat Jr., R.J.,Stoddard, B.L.
DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI.
Mol.Cell, 2:469-476, 1998

PubMed Abstract: The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation.

PubMed: 9809068
DOI: 10.1016/S1097-2765(00)80146-X

Experimental method

X-RAY DIFFRACTION (3 Å)