1BP7
GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.556, 89.259, 177.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 3.000 |
| R-factor | 0.242 * |
| Rwork | 0.234 |
| R-free | 0.28000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1AF5 DIMER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.322 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.042 * | 0.297 * |
| Total number of observations | 86360 * | |
| Number of reflections | 20643 * | |
| <I/σ(I)> | 7.4 | 3.1 |
| Completeness [%] | 96.6 | 79.6 |
| Redundancy | 4.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | CACL2 | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | 1-3-PROPANEDIOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | DNA | 2:1 molar ratio of DNA:protein | |
| 3 | 1 | reservoir | PEG3350 | 14 (%) | |
| 4 | 1 | reservoir | MES | 100 (mM) | |
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | 1-3 propanediol | 0.2 (%) |
