8KAL
Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA
Summary for 8KAL
Entry DOI | 10.2210/pdb8kal/pdb |
Descriptor | RNA (98-MER), CRISPR-associated endonuclease Cas9/Csn1, DNA (25-MER), ... (6 entities in total) |
Functional Keywords | nuclease, rna binding protein/rna/dna, rna binding protein-rna-dna complex |
Biological source | Streptococcus pyogenes serotype M1 More |
Total number of polymer chains | 8 |
Total formula weight | 402601.53 |
Authors | |
Primary citation | Chen, J.,Chen, Y.,Huang, L.,Lin, X.,Chen, H.,Xiang, W.,Liu, L. Trans-nuclease activity of Cas9 activated by DNA or RNA target binding. Nat.Biotechnol., 2024 Cited by PubMed: 38811761DOI: 10.1038/s41587-024-02255-7 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.16 Å) |
Structure validation
Download full validation report