8KAL

Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0003677	molecular_function	DNA binding
B	0003723	molecular_function	RNA binding
B	0004519	molecular_function	endonuclease activity
B	0004520	molecular_function	DNA endonuclease activity
B	0004527	molecular_function	exonuclease activity
B	0008408	molecular_function	3'-5' exonuclease activity
B	0043571	biological_process	maintenance of CRISPR repeat elements
B	0046872	molecular_function	metal ion binding
B	0051607	biological_process	defense response to virus
G	0003677	molecular_function	DNA binding
G	0003723	molecular_function	RNA binding
G	0004519	molecular_function	endonuclease activity
G	0004520	molecular_function	DNA endonuclease activity
G	0004527	molecular_function	exonuclease activity
G	0008408	molecular_function	3'-5' exonuclease activity
G	0043571	biological_process	maintenance of CRISPR repeat elements
G	0046872	molecular_function	metal ion binding
G	0051607	biological_process	defense response to virus

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: For RuvC-like nuclease domain => ECO:0000305|PubMed:24529477

Chain	Residue	Details
B	ALA10
G	ALA10

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site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:24529477

Chain	Residue	Details
B	ALA840
G	ALA840

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site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:24505130

Chain	Residue	Details
B	ALA10
G	HIS983
G	HIS1297
G	ASP1328
B	GLU762
B	GLU766
B	HIS983
B	HIS1297
B	ASP1328
G	ALA10
G	GLU762
G	GLU766

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