8KAL
Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | RNA (98-MER) | polymer | 98 | 31610.7 | 2 | Streptococcus pyogenes serotype M1 | ||
| 2 | B, F (B, G) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158588.8 | 2 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes serotype M1 | SpCas9,SpyCas9 |
| 3 | C, G (C, H) | DNA (25-MER) | polymer | 25 | 7611.0 | 2 | Streptococcus pyogenes serotype M1 | ||
| 4 | D, H (D, J) | DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3') | polymer | 11 | 3394.2 | 2 | Streptococcus pyogenes serotype M1 | ||
| 5 | I, J (B, G) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 6 | K, L, M, N, O... (A, B, C, E, G...) | water | water | 18.0 | 63 | Chemie (HOH) |
Sequence modifications
B, G: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 402409.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 192.1 | |
| All* | Total formula weight | 402601.5 |
