8KAL
Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 362.049, 70.956, 200.100 |
| Unit cell angles | 90.00, 101.52, 90.00 |
Refinement procedure
| Resolution | 50.170 - 3.160 |
| R-factor | 0.2526 |
| Rwork | 0.251 |
| R-free | 0.28840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.557 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.170 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rpim | 0.187 | 0.540 |
| Number of reflections | 83075 | 4098 |
| <I/σ(I)> | 3 | |
| Completeness [%] | 99.1 | 98 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Ammonium sulfate, 0.1 M MES pH 6.0, 12.625% PEG8000 |
