8J1J

Cryo-EM structure of the AsCas12f-YHAM-sgRNAS3-5v7-target DNA

Summary for 8J1J

• Entry DOI: 10.2210/pdb8j1j/pdb
• EMDB information: 35926
• Descriptor: Transposase IS605 OrfB C-terminal domain-containing protein, DNA (38-MER), RNA (118-MER), ... (6 entities in total)
• Functional Keywords: crispr-cas, rna binding protein-dna complex, rna binding protein, rna binding protein-dna-rna complex, rna binding protein/dna/rna
• Biological source: Sulfoacidibacillus thermotolerans; More
• Total number of polymer chains: 5
• Total formula weight: 161784.55

Authors

Hino, T.,Omura, N.S.,Nakagawa, R.,Togashi, T.,Takeda, N.S.,Hiramoto, T.,Tasaka, S.,Hirano, H.,Tokuyama, T.,Uosaki, H.,Ishiguro, H.,Yamano, H.,Ozaki, Y.,Motooka, D.,Mori, H.,Kirita, Y.,Kise, Y.,Itoh, Y.,Matoba, S.,Aburatani, H.,Yachie, N.,Siksnys, V.,Ohmori, T.,Hoshino, A.,Nureki, O. (deposition date: 2023-04-13, release date: 2023-09-27)

• Primary citation: Hino, T.,Omura, N.S.,Nakagawa, R.,Togashi, T.,Takeda, N.S.,Hiramoto, T.,Tasaka, S.,Hirano, H.,Tokuyama, T.,Uosaki, H.,Ishiguro, H.,Yamano, H.,Ozaki, Y.,Motooka, D.,Mori, H.,Kirita, Y.,Kise, Y.,Itoh, Y.,Matoba, S.,Aburatani, H.,Yachie, N.,Siksnys, V.,Ohmori, T.,Hoshino, A.,Nureki, O.; Minimal and most efficient genome editing Cas enzyme; To Be Published,

Experimental method

ELECTRON MICROSCOPY (2.91 Å)