8J1J
Cryo-EM structure of the AsCas12f-YHAM-sgRNAS3-5v7-target DNA
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0004518 | molecular_function | nuclease activity |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 125 |
| Details | Region: {"description":"Recognition domain (REC)","evidences":[{"source":"UniProtKB","id":"A0A482D308","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 168 |
| Details | Region: {"description":"Wedge domain (WED)","evidences":[{"source":"UniProtKB","id":"A0A482D308","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | Region: {"description":"Linker","evidences":[{"source":"UniProtKB","id":"A0A482D308","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 149 |
| Details | Region: {"description":"RuvC-I","evidences":[{"source":"UniProtKB","id":"A0A482D308","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 28 |
| Details | Region: {"description":"Target nucleic acid-binding (TNB)","evidences":[{"source":"UniProtKB","id":"A0A482D308","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 40 |
| Details | Region: {"description":"RuvC-II","evidences":[{"source":"UniProtKB","id":"A0A482D308","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | Active site: {"evidences":[{"source":"UniProtKB","id":"A0A482D308","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"A0A482D308","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
