8J1J

Cryo-EM structure of the AsCas12f-YHAM-sgRNAS3-5v7-target DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, B)	Transposase IS605 OrfB C-terminal domain-containing protein	polymer	432	49966.1	2	UniProt (A0A2U3D0N8) Pfam (PF07282)	Sulfoacidibacillus thermotolerans	AsCas12f-YHAM
2	B (D)	DNA (38-MER)	polymer	38	11694.6	1		Sulfoacidibacillus thermotolerans
3	C (E)	DNA (38-MER)	polymer	38	11689.5	1		Sulfoacidibacillus thermotolerans
4	D (C)	RNA (118-MER)	polymer	118	38288.8	1		Sulfoacidibacillus thermotolerans
5	F, I (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	G, H (C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 1 - 422 (UniProt: A0A2U3D0N8)

PDB	External Database	Details
Met -9	-	initiating methionine
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Tyr 48	Phe 48	engineered mutation
His 188	Ser 188	engineered mutation
Ala 232	Val 232	engineered mutation
Met 316	Glu 316	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	161605.1
Non-Polymers*	Number of molecules	4
	Total formula weight	179.4
All*	Total formula weight	161784.6

*Water molecules are not included.