8D2Q

Structure of Acidothermus cellulolyticus Cas9 ternary complex (Post-cleavage 1)

Summary for 8D2Q

• Entry DOI: 10.2210/pdb8d2q/pdb
• Related: 8D2P
• EMDB information: 27145 27146
• Descriptor: CRISPR-associated endonuclease, Csn1 family, Single Guide RNA (86-MER), DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*G)-3'), ... (7 entities in total)
• Functional Keywords: cas9, acecas9, crispr, post-cleavage 1, rna binding protein, rna binding protein-dna-rna complex, rna binding protein/dna/rna
• Biological source: Acidothermus cellulolyticus 11B; More
• Total number of polymer chains: 5
• Total formula weight: 172996.13

Authors

Rai, J.,Das, A.,Li, H. (deposition date: 2022-05-30, release date: 2023-12-20)

• Primary citation: Das, A.,Rai, J.,Roth, M.O.,Shu, Y.,Medina, M.L.,Barakat, M.R.,Li, H.; Coupled catalytic states and the role of metal coordination in Cas9; Nat Catal, 6:969-977, 2023; DOI: 10.1038/s41929-023-01031-1

Experimental method

ELECTRON MICROSCOPY (2.58 Å)