8D2P

Structure of Acidothermus cellulolyticus Cas9 ternary complex (Target bound)

Summary for 8D2P

• Entry DOI: 10.2210/pdb8d2p/pdb
• Related: 8D2O
• EMDB information: 27144 27145
• Descriptor: CRISPR-associated endonuclease, Csn1 family, Single guide RNA (102-MER), DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*GP*CP*CP*AP*TP*CP*CP*TP*AP*CP*CP*TP*CP*T)-3'), ... (5 entities in total)
• Functional Keywords: cas9, acecas9, crispr, target bound, rna binding protein, rna binding protein-dna-rna complex, rna binding protein/dna/rna
• Biological source: Acidothermus cellulolyticus 11B; More
• Total number of polymer chains: 3
• Total formula weight: 169348.09

Authors

Rai, J.,Das, A.,Li, H. (deposition date: 2022-05-30, release date: 2023-12-20, Last modification date: 2024-05-01)

• Primary citation: Das, A.,Rai, J.,Roth, M.O.,Shu, Y.,Medina, M.L.,Barakat, M.R.,Li, H.; Coupled catalytic states and the role of metal coordination in Cas9.; Nat Catal, 6:969-977, 2023; PubMed: 38348449; DOI: 10.1038/s41929-023-01031-1

Experimental method

ELECTRON MICROSCOPY (2.78 Å)