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8D2Q

Structure of Acidothermus cellulolyticus Cas9 ternary complex (Post-cleavage 1)

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
CRISPR-associated endonuclease, Csn1 familypolymer1138127498.11UniProt (A0LWB3)
Pfam (PF18470)
Pfam (PF01844)
Pfam (PF18541)
Pfam (PF17893)
Pfam (PF17894)
Pfam (PF18525)
Acidothermus cellulolyticus 11B
2B
(B)
Single Guide RNA (86-MER)polymer10634390.31Acidothermus cellulolyticus 11B
3C
(T)
DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*G)-3')polymer113349.21synthetic construct
4D
(X)
DNA target strand (5'-D(*AP*GP*CP*TP*TP*GP*GP*TP*GP*TP*AP*TP*A)-3')polymer134021.61synthetic construct
5E
(D)
DNA non-target strand (5'-D(P*AP*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3')polymer123615.41synthetic construct
6F, G, H, I, J
(A, B)
MAGNESIUM IONnon-polymer24.35Chemie (MG)
7K
(A)
waterwater18.02Chemie (HOH)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains5
Total formula weight172874.6
Non-Polymers*Number of molecules5
Total formula weight121.5
All*Total formula weight172996.1
*Water molecules are not included.

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