8D2Q
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Post-cleavage 1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease, Csn1 family | polymer | 1138 | 127498.1 | 1 | UniProt (A0LWB3) Pfam (PF18470) Pfam (PF01844) Pfam (PF18541) Pfam (PF17893) Pfam (PF17894) Pfam (PF18525) | Acidothermus cellulolyticus 11B | |
| 2 | B (B) | Single Guide RNA (86-MER) | polymer | 106 | 34390.3 | 1 | Acidothermus cellulolyticus 11B | ||
| 3 | C (T) | DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*G)-3') | polymer | 11 | 3349.2 | 1 | synthetic construct | ||
| 4 | D (X) | DNA target strand (5'-D(*AP*GP*CP*TP*TP*GP*GP*TP*GP*TP*AP*TP*A)-3') | polymer | 13 | 4021.6 | 1 | synthetic construct | ||
| 5 | E (D) | DNA non-target strand (5'-D(P*AP*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3') | polymer | 12 | 3615.4 | 1 | synthetic construct | ||
| 6 | F, G, H, I, J (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 7 | K (A) | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 172874.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 121.5 | |
| All* | Total formula weight | 172996.1 |
