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7ZNL

Structure of the human TREX core THO-UAP56 complex

Summary for 7ZNL
Entry DOI10.2210/pdb7znl/pdb
Related7ZNJ 7ZNK
EMDB information11853 11854 11857 13809 14808 17215
DescriptorTHO complex subunit 1, THO complex subunit 2, THO complex subunit 3, ... (7 entities in total)
Functional Keywordstranscription and export complex, trex, rna export, rna packaging, rna binding protein, gene expression, gene regulation
Biological sourceHomo sapiens (human)
More
Total number of polymer chains28
Total formula weight1946793.99
Authors
Pacheco-Fiallos, F.B.,Vorlaender, M.K.,Plaschka, C. (deposition date: 2022-04-21, release date: 2023-05-17, Last modification date: 2024-07-24)
Primary citationPacheco-Fiallos, B.,Vorlander, M.K.,Riabov-Bassat, D.,Fin, L.,O'Reilly, F.J.,Ayala, F.I.,Schellhaas, U.,Rappsilber, J.,Plaschka, C.
mRNA recognition and packaging by the human transcription-export complex.
Nature, 616:828-835, 2023
Cited by
PubMed Abstract: Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the mechanisms of mRNP recognition and three-dimensional mRNP organization are poorly understood. Here we report cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNPs bound to the 2-MDa TREX complex. We show that mRNPs are recognized through multivalent interactions between the TREX subunit ALYREF and mRNP-bound exon junction complexes. Exon junction complexes can multimerize through ALYREF, which suggests a mechanism for mRNP organization. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes. These results reveal how TREX may simultaneously recognize, compact and protect mRNAs to promote their packaging for nuclear export. The organization of mRNP globules provides a framework to understand how mRNP architecture facilitates mRNA biogenesis and export.
PubMed: 37020021
DOI: 10.1038/s41586-023-05904-0
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (3.45 Å)
Structure validation

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