7ZNK

Structure of an endogenous human TREX complex bound to mRNA

7ZNK の概要

• エントリーDOI: 10.2210/pdb7znk/pdb
• 関連するPDBエントリー: 7ZNJ
• EMDBエントリー: 14804 14805 14806 14807 14808 14809
• 分子名称: RNA, THO complex subunit 1, THO complex subunit 2, ... (9 entities in total)
• 機能のキーワード: transcription and export complex, trex, rna export, rna packaging, rna binding protein, gene expression, gene regulation
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 2002521.26

構造登録者

Pacheco-Fiallos, F.B.,Vorlaender, M.K.,Plaschka, C. (登録日: 2022-04-21, 公開日: 2023-05-03, 最終更新日: 2024-07-24)

主引用文献

Pacheco-Fiallos, B.,Vorlander, M.K.,Riabov-Bassat, D.,Fin, L.,O'Reilly, F.J.,Ayala, F.I.,Schellhaas, U.,Rappsilber, J.,Plaschka, C.
mRNA recognition and packaging by the human transcription-export complex.
Nature, 616:828-835, 2023

PubMed Abstract: Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the mechanisms of mRNP recognition and three-dimensional mRNP organization are poorly understood. Here we report cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNPs bound to the 2-MDa TREX complex. We show that mRNPs are recognized through multivalent interactions between the TREX subunit ALYREF and mRNP-bound exon junction complexes. Exon junction complexes can multimerize through ALYREF, which suggests a mechanism for mRNP organization. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes. These results reveal how TREX may simultaneously recognize, compact and protect mRNAs to promote their packaging for nuclear export. The organization of mRNP globules provides a framework to understand how mRNP architecture facilitates mRNA biogenesis and export.

PubMed: 37020021
DOI: 10.1038/s41586-023-05904-0

実験手法

ELECTRON MICROSCOPY (3.9 Å)