7ZNJ

Structure of an ALYREF-exon junction complex hexamer

Summary for 7ZNJ

• Entry DOI: 10.2210/pdb7znj/pdb
• Related: 7ZNK
• EMDB information: 14803 16633
• Descriptor: Eukaryotic initiation factor 4A-III, N-terminally processed, Protein mago nashi homolog, RNA-binding protein 8A, ... (7 entities in total)
• Functional Keywords: transcription-exort complex, trex, splicing, exon junction complex, ejc, rna export, rna binding proteins, gene regulation
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 30
• Total formula weight: 787805.46

Authors

Pacheco-Fiallos, F.B.,Vorlaender, M.K.,Plaschka, C. (deposition date: 2022-04-21, release date: 2023-04-12, Last modification date: 2024-07-24)

Primary citation

Pacheco-Fiallos, B.,Vorlander, M.K.,Riabov-Bassat, D.,Fin, L.,O'Reilly, F.J.,Ayala, F.I.,Schellhaas, U.,Rappsilber, J.,Plaschka, C.
mRNA recognition and packaging by the human transcription-export complex.
Nature, 616:828-835, 2023

PubMed Abstract: Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the mechanisms of mRNP recognition and three-dimensional mRNP organization are poorly understood. Here we report cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNPs bound to the 2-MDa TREX complex. We show that mRNPs are recognized through multivalent interactions between the TREX subunit ALYREF and mRNP-bound exon junction complexes. Exon junction complexes can multimerize through ALYREF, which suggests a mechanism for mRNP organization. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes. These results reveal how TREX may simultaneously recognize, compact and protect mRNAs to promote their packaging for nuclear export. The organization of mRNP globules provides a framework to understand how mRNP architecture facilitates mRNA biogenesis and export.

PubMed: 37020021
DOI: 10.1038/s41586-023-05904-0

Experimental method

ELECTRON MICROSCOPY (2.4 Å)