7PFP

Full-length cryo-EM structure of the native human uromodulin (UMOD)/Tamm-Horsfall protein (THP) filament

7PFP の概要

• エントリーDOI: 10.2210/pdb7pfp/pdb
• 関連するPDBエントリー: 4WRN 6TQK 6TQL 7P6R 7P6S 7P6T
• EMDBエントリー: 10553 10554 13378
• 分子名称: Uromodulin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: egf domain, decoy module, beta-hairpin, d10c domain, zp module, zp domain, zp-n domain, zp-c domain, interdomain linker, extracellular matrix, glycoprotein, n-glycan, structural protein, protein filament, protein polymerization, antimicrobial protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 224801.05

構造登録者

Jovine, L.,Xu, C.,Stsiapanava, A.,Carroni, M.,Tunyasuvunakool, K.,Jumper, J.,Wu, B. (登録日: 2021-08-11, 公開日: 2022-03-16, 最終更新日: 2024-10-16)

主引用文献

Stsiapanava, A.,Xu, C.,Nishio, S.,Han, L.,Yamakawa, N.,Carroni, M.,Tunyasuvunakool, K.,Jumper, J.,de Sanctis, D.,Wu, B.,Jovine, L.
Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH.
Nat.Struct.Mol.Biol., 29:190-193, 2022

PubMed Abstract: Glycoprotein 2 (GP2) and uromodulin (UMOD) filaments protect against gastrointestinal and urinary tract infections by acting as decoys for bacterial fimbrial lectin FimH. By combining AlphaFold2 predictions with X-ray crystallography and cryo-EM, we show that these proteins contain a bipartite decoy module whose new fold presents the high-mannose glycan recognized by FimH. The structure rationalizes UMOD mutations associated with kidney diseases and visualizes a key epitope implicated in cast nephropathy.

PubMed: 35273390
DOI: 10.1038/s41594-022-00729-3

実験手法

ELECTRON MICROSCOPY (6.1 Å)